Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05125 and MMJJ_RS05130 are separated by 36 nucleotides MMJJ_RS05130 and MMJJ_RS05135 are separated by 18 nucleotides MMJJ_RS05135 and MMJJ_RS05140 are separated by 108 nucleotides MMJJ_RS05140 and MMJJ_RS05145 are separated by 62 nucleotides
MMJJ_RS05125: MMJJ_RS05125 - 50S ribosomal protein L31e, at 950,278 to 950,529
_RS05125
MMJJ_RS05130: MMJJ_RS05130 - translation initiation factor IF-6, at 950,566 to 951,249
_RS05130
MMJJ_RS05135: MMJJ_RS05135 - 50S ribosomal protein L18Ae, at 951,268 to 951,498
_RS05135
MMJJ_RS05140: MMJJ_RS05140 - DUF2666 domain-containing protein, at 951,607 to 952,029
_RS05140
MMJJ_RS05145: MMJJ_RS05145 - 5,10-methylenetetrahydromethanopterin reductase, at 952,092 to 953,054
_RS05145
Position (kb) 951
952 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 950.889 kb on + strand, within MMJJ_RS05130 at 950.960 kb on - strand, within MMJJ_RS05130 at 951.243 kb on + strand at 951.248 kb on + strand at 951.264 kb on - strand at 951.265 kb on + strand at 951.449 kb on - strand, within MMJJ_RS05135 at 951.496 kb on - strand at 951.496 kb on - strand at 951.505 kb on - strand at 951.514 kb on - strand at 951.515 kb on + strand at 951.540 kb on - strand at 951.545 kb on - strand at 951.546 kb on + strand at 951.547 kb on - strand at 951.547 kb on - strand at 951.550 kb on + strand at 951.554 kb on - strand at 951.563 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.577 kb on - strand at 951.579 kb on - strand at 951.579 kb on - strand at 951.580 kb on + strand at 951.583 kb on + strand at 951.606 kb on - strand at 951.623 kb on - strand at 951.653 kb on + strand, within MMJJ_RS05140 at 951.684 kb on - strand, within MMJJ_RS05140 at 951.801 kb on + strand, within MMJJ_RS05140 at 951.825 kb on - strand, within MMJJ_RS05140 at 951.825 kb on - strand, within MMJJ_RS05140 at 951.838 kb on + strand, within MMJJ_RS05140 at 951.877 kb on + strand, within MMJJ_RS05140 at 951.903 kb on - strand, within MMJJ_RS05140 at 951.954 kb on + strand, within MMJJ_RS05140 at 951.987 kb on - strand at 951.988 kb on + strand at 952.004 kb on + strand at 952.090 kb on + strand at 952.390 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 950,889 + MMJJ_RS05130 0.47 -0.2 950,960 - MMJJ_RS05130 0.58 +0.4 951,243 + -0.0 951,248 + +0.4 951,264 - +0.5 951,265 + -1.3 951,449 - MMJJ_RS05135 0.78 -1.7 951,496 - -0.9 951,496 - -1.7 951,505 - +4.8 951,514 - -0.6 951,515 + -0.8 951,540 - -0.6 951,545 - -2.4 951,546 + +0.6 951,547 - -0.4 951,547 - -1.2 951,550 + +0.0 951,554 - -1.9 951,563 - +0.2 951,565 - +1.4 951,565 - +1.0 951,565 - -0.9 951,565 - -0.9 951,577 - +1.4 951,579 - +0.3 951,579 - +0.8 951,580 + +0.4 951,583 + -1.3 951,606 - -3.5 951,623 - -0.1 951,653 + MMJJ_RS05140 0.11 -0.7 951,684 - MMJJ_RS05140 0.18 +1.1 951,801 + MMJJ_RS05140 0.46 +2.5 951,825 - MMJJ_RS05140 0.52 -2.4 951,825 - MMJJ_RS05140 0.52 +0.8 951,838 + MMJJ_RS05140 0.55 -2.8 951,877 + MMJJ_RS05140 0.64 -0.2 951,903 - MMJJ_RS05140 0.70 -1.5 951,954 + MMJJ_RS05140 0.82 -0.9 951,987 - -1.4 951,988 + -1.8 952,004 + -1.3 952,090 + -0.9 952,390 + -0.2
Or see this region's nucleotide sequence