Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05060 and MMJJ_RS05065 are separated by 132 nucleotides MMJJ_RS05065 and MMJJ_RS05070 are separated by 114 nucleotides
MMJJ_RS05060: MMJJ_RS05060 - argininosuccinate synthase, at 939,506 to 940,699
_RS05060
MMJJ_RS05065: MMJJ_RS05065 - (4-{4-[2-(gamma-L- glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase, at 940,832 to 941,812
_RS05065
MMJJ_RS05070: MMJJ_RS05070 - DNA primase catalytic subunit PriS, at 941,927 to 943,000
_RS05070
Position (kb)
940
941
942 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 940.703 kb on - strand at 940.745 kb on - strand at 940.773 kb on - strand at 940.776 kb on - strand at 940.784 kb on - strand at 940.788 kb on - strand at 940.789 kb on + strand at 940.790 kb on - strand at 940.803 kb on + strand at 940.803 kb on + strand at 940.830 kb on - strand at 940.834 kb on + strand at 940.973 kb on - strand, within MMJJ_RS05065 at 941.027 kb on - strand, within MMJJ_RS05065 at 941.046 kb on - strand, within MMJJ_RS05065 at 941.063 kb on + strand, within MMJJ_RS05065 at 941.213 kb on + strand, within MMJJ_RS05065 at 941.222 kb on - strand, within MMJJ_RS05065 at 941.497 kb on + strand, within MMJJ_RS05065 at 941.696 kb on - strand, within MMJJ_RS05065 at 941.744 kb on + strand at 941.823 kb on - strand at 941.824 kb on + strand at 941.824 kb on + strand at 941.824 kb on + strand at 941.832 kb on + strand at 941.858 kb on - strand at 941.858 kb on - strand at 941.859 kb on + strand at 941.864 kb on - strand at 941.864 kb on - strand at 941.865 kb on + strand at 941.865 kb on + strand at 941.870 kb on - strand at 941.871 kb on + strand at 941.871 kb on + strand at 941.874 kb on - strand at 941.874 kb on - strand at 941.890 kb on - strand at 941.891 kb on + strand at 942.158 kb on + strand, within MMJJ_RS05070 at 942.254 kb on + strand, within MMJJ_RS05070 at 942.268 kb on - strand, within MMJJ_RS05070 at 942.390 kb on - strand, within MMJJ_RS05070 at 942.390 kb on - strand, within MMJJ_RS05070 at 942.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 940,703 - +1.3 940,745 - -1.7 940,773 - -0.5 940,776 - -2.0 940,784 - -0.2 940,788 - -1.2 940,789 + -1.8 940,790 - +1.3 940,803 + -1.4 940,803 + -2.2 940,830 - +2.2 940,834 + -2.2 940,973 - MMJJ_RS05065 0.14 -2.4 941,027 - MMJJ_RS05065 0.20 -0.7 941,046 - MMJJ_RS05065 0.22 -1.0 941,063 + MMJJ_RS05065 0.24 -0.7 941,213 + MMJJ_RS05065 0.39 -2.0 941,222 - MMJJ_RS05065 0.40 -0.2 941,497 + MMJJ_RS05065 0.68 -2.5 941,696 - MMJJ_RS05065 0.88 -0.8 941,744 + +0.3 941,823 - -1.6 941,824 + -0.8 941,824 + -3.2 941,824 + -0.7 941,832 + -2.0 941,858 - -1.1 941,858 - -2.7 941,859 + -2.9 941,864 - -1.5 941,864 - +0.3 941,865 + -0.7 941,865 + -2.9 941,870 - +0.3 941,871 + -1.6 941,871 + -1.0 941,874 - +2.6 941,874 - -1.7 941,890 - -1.4 941,891 + +1.7 942,158 + MMJJ_RS05070 0.22 -0.2 942,254 + MMJJ_RS05070 0.30 +1.3 942,268 - MMJJ_RS05070 0.32 -2.1 942,390 - MMJJ_RS05070 0.43 -0.2 942,390 - MMJJ_RS05070 0.43 -0.7 942,417 - +1.3
Or see this region's nucleotide sequence