Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04740 and MMJJ_RS04745 are separated by 77 nucleotides MMJJ_RS04745 and MMJJ_RS04750 are separated by 305 nucleotides MMJJ_RS04750 and MMJJ_RS04755 are separated by 59 nucleotides
MMJJ_RS04740: MMJJ_RS04740 - histidinol phosphate phosphatase domain-containing protein, at 889,321 to 889,977
_RS04740
MMJJ_RS04745: MMJJ_RS04745 - cyclase family protein, at 890,055 to 890,624
_RS04745
MMJJ_RS04750: MMJJ_RS04750 - 5,10-methenyltetrahydromethanopterin hydrogenase, at 890,930 to 891,994
_RS04750
MMJJ_RS04755: MMJJ_RS04755 - 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB, at 892,054 to 893,079
_RS04755
Position (kb) 890
891
892 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 890.014 kb on - strand at 890.015 kb on + strand at 890.015 kb on + strand at 890.016 kb on - strand at 890.016 kb on - strand at 890.017 kb on + strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.021 kb on + strand at 890.023 kb on - strand at 890.026 kb on - strand at 890.026 kb on - strand at 890.031 kb on + strand at 890.034 kb on - strand at 890.034 kb on - strand at 890.042 kb on + strand at 890.071 kb on - strand at 890.072 kb on + strand at 890.104 kb on - strand at 890.104 kb on - strand at 890.155 kb on + strand, within MMJJ_RS04745 at 890.226 kb on + strand, within MMJJ_RS04745 at 890.268 kb on + strand, within MMJJ_RS04745 at 890.353 kb on + strand, within MMJJ_RS04745 at 890.363 kb on - strand, within MMJJ_RS04745 at 890.364 kb on + strand, within MMJJ_RS04745 at 890.364 kb on + strand, within MMJJ_RS04745 at 890.370 kb on - strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.414 kb on + strand, within MMJJ_RS04745 at 890.421 kb on + strand, within MMJJ_RS04745 at 890.489 kb on + strand, within MMJJ_RS04745 at 890.507 kb on + strand, within MMJJ_RS04745 at 890.516 kb on - strand, within MMJJ_RS04745 at 890.517 kb on + strand, within MMJJ_RS04745 at 890.604 kb on + strand at 890.643 kb on + strand at 890.668 kb on + strand at 890.710 kb on + strand at 890.713 kb on + strand at 890.726 kb on - strand at 890.756 kb on + strand at 890.763 kb on + strand at 890.765 kb on - strand at 890.811 kb on + strand at 890.813 kb on + strand at 890.816 kb on - strand at 890.822 kb on - strand at 890.858 kb on - strand at 890.858 kb on - strand at 890.859 kb on + strand at 890.886 kb on - strand at 890.887 kb on + strand at 890.887 kb on + strand at 890.895 kb on - strand at 890.896 kb on + strand at 890.901 kb on + strand at 890.901 kb on + strand at 890.904 kb on + strand at 890.905 kb on - strand at 890.905 kb on - strand at 890.905 kb on - strand at 890.906 kb on + strand at 890.906 kb on + strand at 890.906 kb on + strand at 890.910 kb on - strand at 890.910 kb on - strand at 890.910 kb on - strand at 890.914 kb on - strand at 890.915 kb on + strand at 890.924 kb on - strand at 890.924 kb on - strand at 890.925 kb on + strand at 890.925 kb on + strand at 890.930 kb on + strand at 890.943 kb on - strand at 890.947 kb on + strand at 890.947 kb on + strand at 890.947 kb on + strand at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.088 kb on + strand, within MMJJ_RS04750 at 891.100 kb on - strand, within MMJJ_RS04750 at 891.151 kb on + strand, within MMJJ_RS04750 at 891.153 kb on - strand, within MMJJ_RS04750 at 891.154 kb on + strand, within MMJJ_RS04750 at 891.182 kb on + strand, within MMJJ_RS04750 at 891.196 kb on + strand, within MMJJ_RS04750 at 891.241 kb on + strand, within MMJJ_RS04750 at 891.253 kb on - strand, within MMJJ_RS04750 at 891.295 kb on + strand, within MMJJ_RS04750 at 891.295 kb on + strand, within MMJJ_RS04750 at 891.364 kb on + strand, within MMJJ_RS04750 at 891.367 kb on - strand, within MMJJ_RS04750 at 891.367 kb on - strand, within MMJJ_RS04750 at 891.368 kb on + strand, within MMJJ_RS04750 at 891.368 kb on + strand, within MMJJ_RS04750 at 891.402 kb on - strand, within MMJJ_RS04750 at 891.445 kb on + strand, within MMJJ_RS04750 at 891.458 kb on + strand, within MMJJ_RS04750 at 891.514 kb on + strand, within MMJJ_RS04750 at 891.527 kb on - strand, within MMJJ_RS04750 at 891.583 kb on + strand, within MMJJ_RS04750 at 891.611 kb on - strand, within MMJJ_RS04750 at 891.611 kb on - strand, within MMJJ_RS04750 at 891.616 kb on - strand, within MMJJ_RS04750 at 891.663 kb on - strand, within MMJJ_RS04750 at 891.697 kb on - strand, within MMJJ_RS04750 at 891.754 kb on + strand, within MMJJ_RS04750 at 891.862 kb on - strand, within MMJJ_RS04750 at 891.913 kb on - strand at 891.914 kb on + strand at 891.914 kb on + strand at 891.992 kb on - strand at 891.997 kb on + strand at 891.997 kb on + strand at 892.000 kb on + strand at 892.002 kb on - strand at 892.003 kb on + strand at 892.009 kb on - strand at 892.010 kb on + strand at 892.038 kb on + strand at 892.059 kb on + strand at 892.070 kb on - strand at 892.107 kb on + strand at 892.112 kb on - strand at 892.112 kb on - strand at 892.125 kb on + strand at 892.152 kb on + strand at 892.155 kb on - strand at 892.156 kb on + strand at 892.181 kb on - strand, within MMJJ_RS04755 at 892.182 kb on + strand, within MMJJ_RS04755 at 892.210 kb on + strand at 892.210 kb on + strand, within MMJJ_RS04755 at 892.256 kb on - strand, within MMJJ_RS04755 at 892.256 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.264 kb on + strand, within MMJJ_RS04755 at 892.291 kb on - strand, within MMJJ_RS04755 at 892.292 kb on + strand, within MMJJ_RS04755 at 892.328 kb on + strand, within MMJJ_RS04755 at 892.331 kb on - strand, within MMJJ_RS04755 at 892.331 kb on - strand, within MMJJ_RS04755 at 892.332 kb on + strand, within MMJJ_RS04755 at 892.332 kb on + strand, within MMJJ_RS04755 at 892.445 kb on - strand, within MMJJ_RS04755 at 892.457 kb on - strand, within MMJJ_RS04755 at 892.484 kb on - strand, within MMJJ_RS04755 at 892.514 kb on - strand, within MMJJ_RS04755 at 892.546 kb on - strand, within MMJJ_RS04755 at 892.548 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand at 892.557 kb on - strand, within MMJJ_RS04755 at 892.558 kb on + strand, within MMJJ_RS04755 at 892.558 kb on + strand, within MMJJ_RS04755 at 892.563 kb on + strand, within MMJJ_RS04755 at 892.637 kb on - strand, within MMJJ_RS04755 at 892.671 kb on + strand, within MMJJ_RS04755 at 892.676 kb on - strand, within MMJJ_RS04755 at 892.694 kb on - strand, within MMJJ_RS04755 at 892.699 kb on + strand, within MMJJ_RS04755 at 892.719 kb on - strand, within MMJJ_RS04755 at 892.720 kb on + strand, within MMJJ_RS04755 at 892.773 kb on + strand, within MMJJ_RS04755 at 892.790 kb on + strand, within MMJJ_RS04755 at 892.860 kb on + strand, within MMJJ_RS04755 at 892.888 kb on + strand, within MMJJ_RS04755 at 892.964 kb on - strand, within MMJJ_RS04755 at 892.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 890,014 - +1.3 890,015 + -2.1 890,015 + +0.2 890,016 - +2.0 890,016 - -1.7 890,017 + +0.4 890,020 - +1.0 890,020 - -1.5 890,020 - -1.4 890,020 - +2.3 890,020 - -2.0 890,020 - -2.4 890,021 + -1.7 890,023 - -1.2 890,026 - +0.8 890,026 - -1.8 890,031 + -2.3 890,034 - +1.4 890,034 - +0.7 890,042 + -0.2 890,071 - +1.8 890,072 + -1.0 890,104 - +0.5 890,104 - -1.5 890,155 + MMJJ_RS04745 0.18 -1.0 890,226 + MMJJ_RS04745 0.30 +1.1 890,268 + MMJJ_RS04745 0.37 -2.1 890,353 + MMJJ_RS04745 0.52 -2.0 890,363 - MMJJ_RS04745 0.54 +2.5 890,364 + MMJJ_RS04745 0.54 +1.9 890,364 + MMJJ_RS04745 0.54 +0.2 890,370 - MMJJ_RS04745 0.55 +1.3 890,371 + MMJJ_RS04745 0.55 -0.9 890,371 + MMJJ_RS04745 0.55 -2.4 890,371 + MMJJ_RS04745 0.55 -0.9 890,414 + MMJJ_RS04745 0.63 +1.2 890,421 + MMJJ_RS04745 0.64 -2.0 890,489 + MMJJ_RS04745 0.76 +0.5 890,507 + MMJJ_RS04745 0.79 +2.1 890,516 - MMJJ_RS04745 0.81 +2.1 890,517 + MMJJ_RS04745 0.81 +2.0 890,604 + +0.3 890,643 + -3.2 890,668 + -3.1 890,710 + +0.1 890,713 + +0.0 890,726 - +0.4 890,756 + +3.3 890,763 + -0.2 890,765 - -1.1 890,811 + -2.7 890,813 + +1.7 890,816 - -0.2 890,822 - -1.6 890,858 - -1.8 890,858 - -0.6 890,859 + -1.7 890,886 - -1.1 890,887 + -1.7 890,887 + -2.1 890,895 - -2.1 890,896 + -2.6 890,901 + -2.7 890,901 + -2.6 890,904 + -1.9 890,905 - -0.2 890,905 - -2.7 890,905 - -2.8 890,906 + -2.5 890,906 + +0.6 890,906 + +1.9 890,910 - -1.0 890,910 - -2.6 890,910 - -1.3 890,914 - -2.1 890,915 + -2.3 890,924 - -2.1 890,924 - -1.6 890,925 + -2.3 890,925 + -3.2 890,930 + -2.3 890,943 - -1.7 890,947 + -3.4 890,947 + -0.6 890,947 + -3.9 891,087 - MMJJ_RS04750 0.15 -1.6 891,087 - MMJJ_RS04750 0.15 -2.6 891,087 - MMJJ_RS04750 0.15 -1.4 891,087 - MMJJ_RS04750 0.15 -2.3 891,088 + MMJJ_RS04750 0.15 -3.1 891,100 - MMJJ_RS04750 0.16 +0.6 891,151 + MMJJ_RS04750 0.21 -0.6 891,153 - MMJJ_RS04750 0.21 -2.8 891,154 + MMJJ_RS04750 0.21 -1.9 891,182 + MMJJ_RS04750 0.24 -2.7 891,196 + MMJJ_RS04750 0.25 -2.2 891,241 + MMJJ_RS04750 0.29 -1.9 891,253 - MMJJ_RS04750 0.30 -1.9 891,295 + MMJJ_RS04750 0.34 -3.8 891,295 + MMJJ_RS04750 0.34 -3.3 891,364 + MMJJ_RS04750 0.41 -1.5 891,367 - MMJJ_RS04750 0.41 -0.6 891,367 - MMJJ_RS04750 0.41 -0.9 891,368 + MMJJ_RS04750 0.41 -1.6 891,368 + MMJJ_RS04750 0.41 -1.8 891,402 - MMJJ_RS04750 0.44 -2.7 891,445 + MMJJ_RS04750 0.48 -1.8 891,458 + MMJJ_RS04750 0.50 -1.6 891,514 + MMJJ_RS04750 0.55 -2.5 891,527 - MMJJ_RS04750 0.56 -1.6 891,583 + MMJJ_RS04750 0.61 -0.6 891,611 - MMJJ_RS04750 0.64 -0.1 891,611 - MMJJ_RS04750 0.64 -3.2 891,616 - MMJJ_RS04750 0.64 -0.2 891,663 - MMJJ_RS04750 0.69 -0.6 891,697 - MMJJ_RS04750 0.72 -1.4 891,754 + MMJJ_RS04750 0.77 -0.6 891,862 - MMJJ_RS04750 0.88 -2.5 891,913 - -2.7 891,914 + -1.9 891,914 + -1.7 891,992 - -1.2 891,997 + -2.4 891,997 + -0.8 892,000 + -2.6 892,002 - -0.6 892,003 + -2.5 892,009 - -1.8 892,010 + -2.3 892,038 + -0.6 892,059 + -3.5 892,070 - -2.3 892,107 + -2.4 892,112 - -1.3 892,112 - -2.2 892,125 + -1.6 892,152 + -4.2 892,155 - -1.9 892,156 + -2.5 892,181 - MMJJ_RS04755 0.12 -1.4 892,182 + MMJJ_RS04755 0.12 -3.4 892,210 + -0.6 892,210 + MMJJ_RS04755 0.15 -3.3 892,256 - MMJJ_RS04755 0.20 -1.5 892,256 - MMJJ_RS04755 0.20 -1.2 892,260 - MMJJ_RS04755 0.20 -0.8 892,260 - MMJJ_RS04755 0.20 -2.4 892,260 - MMJJ_RS04755 0.20 -3.4 892,260 - MMJJ_RS04755 0.20 -0.9 892,261 + MMJJ_RS04755 0.20 -2.4 892,261 + MMJJ_RS04755 0.20 +2.7 892,261 + MMJJ_RS04755 0.20 -2.3 892,264 + MMJJ_RS04755 0.20 -2.7 892,291 - MMJJ_RS04755 0.23 -0.9 892,292 + MMJJ_RS04755 0.23 -0.1 892,328 + MMJJ_RS04755 0.27 -3.0 892,331 - MMJJ_RS04755 0.27 -1.8 892,331 - MMJJ_RS04755 0.27 -1.4 892,332 + MMJJ_RS04755 0.27 -2.4 892,332 + MMJJ_RS04755 0.27 -1.9 892,445 - MMJJ_RS04755 0.38 -0.5 892,457 - MMJJ_RS04755 0.39 -2.6 892,484 - MMJJ_RS04755 0.42 -2.1 892,514 - MMJJ_RS04755 0.45 -3.1 892,546 - MMJJ_RS04755 0.48 -0.2 892,548 - MMJJ_RS04755 0.48 -1.8 892,553 - MMJJ_RS04755 0.49 -2.2 892,553 - MMJJ_RS04755 0.49 -2.2 892,553 - +2.4 892,557 - MMJJ_RS04755 0.49 -2.1 892,558 + MMJJ_RS04755 0.49 +0.5 892,558 + MMJJ_RS04755 0.49 -1.7 892,563 + MMJJ_RS04755 0.50 -0.2 892,637 - MMJJ_RS04755 0.57 +1.4 892,671 + MMJJ_RS04755 0.60 -1.7 892,676 - MMJJ_RS04755 0.61 -1.7 892,694 - MMJJ_RS04755 0.62 -1.8 892,699 + MMJJ_RS04755 0.63 -1.7 892,719 - MMJJ_RS04755 0.65 -2.0 892,720 + MMJJ_RS04755 0.65 -3.1 892,773 + MMJJ_RS04755 0.70 -1.9 892,790 + MMJJ_RS04755 0.72 -3.7 892,860 + MMJJ_RS04755 0.79 -1.2 892,888 + MMJJ_RS04755 0.81 -2.2 892,964 - MMJJ_RS04755 0.89 -2.6 892,992 - -2.5
Or see this region's nucleotide sequence