Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04720 and MMJJ_RS04725 are separated by 72 nucleotides MMJJ_RS04725 and MMJJ_RS04730 are separated by 49 nucleotides MMJJ_RS04730 and MMJJ_RS04735 are separated by 144 nucleotides
MMJJ_RS04720: MMJJ_RS04720 - IMP dehydrogenase, at 884,673 to 886,175
_RS04720
MMJJ_RS04725: MMJJ_RS04725 - branched-chain-amino-acid transaminase, at 886,248 to 887,111
_RS04725
MMJJ_RS04730: MMJJ_RS04730 - tyrosine decarboxylase MfnA, at 887,161 to 888,315
_RS04730
MMJJ_RS04735: MMJJ_RS04735 - fumarate hydratase, at 888,460 to 889,305
_RS04735
Position (kb) 887
888
889 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 886.173 kb on - strand at 886.187 kb on + strand at 886.194 kb on + strand at 886.246 kb on - strand at 886.247 kb on + strand at 886.247 kb on + strand at 886.249 kb on - strand at 886.249 kb on - strand at 886.254 kb on - strand at 886.254 kb on - strand at 886.255 kb on + strand at 886.255 kb on + strand at 886.255 kb on + strand at 886.258 kb on - strand at 886.259 kb on + strand at 886.259 kb on + strand at 886.281 kb on - strand at 886.282 kb on + strand at 886.282 kb on + strand at 886.295 kb on - strand at 886.302 kb on - strand at 886.302 kb on - strand at 886.302 kb on - strand at 886.303 kb on + strand at 886.323 kb on - strand at 886.338 kb on + strand, within MMJJ_RS04725 at 886.338 kb on + strand, within MMJJ_RS04725 at 886.372 kb on + strand, within MMJJ_RS04725 at 886.395 kb on + strand, within MMJJ_RS04725 at 886.417 kb on + strand, within MMJJ_RS04725 at 886.443 kb on - strand, within MMJJ_RS04725 at 886.446 kb on + strand, within MMJJ_RS04725 at 886.451 kb on - strand, within MMJJ_RS04725 at 886.451 kb on - strand, within MMJJ_RS04725 at 886.452 kb on + strand, within MMJJ_RS04725 at 886.476 kb on - strand, within MMJJ_RS04725 at 886.477 kb on + strand, within MMJJ_RS04725 at 886.494 kb on + strand, within MMJJ_RS04725 at 886.557 kb on - strand, within MMJJ_RS04725 at 886.557 kb on - strand, within MMJJ_RS04725 at 886.557 kb on - strand, within MMJJ_RS04725 at 886.557 kb on - strand, within MMJJ_RS04725 at 886.627 kb on - strand, within MMJJ_RS04725 at 886.659 kb on - strand, within MMJJ_RS04725 at 886.660 kb on + strand, within MMJJ_RS04725 at 886.660 kb on + strand, within MMJJ_RS04725 at 886.689 kb on - strand, within MMJJ_RS04725 at 886.698 kb on - strand, within MMJJ_RS04725 at 886.699 kb on + strand, within MMJJ_RS04725 at 886.704 kb on - strand, within MMJJ_RS04725 at 886.726 kb on + strand, within MMJJ_RS04725 at 886.726 kb on + strand, within MMJJ_RS04725 at 886.728 kb on + strand, within MMJJ_RS04725 at 886.728 kb on + strand, within MMJJ_RS04725 at 886.838 kb on - strand, within MMJJ_RS04725 at 886.838 kb on - strand, within MMJJ_RS04725 at 886.838 kb on - strand, within MMJJ_RS04725 at 886.842 kb on - strand, within MMJJ_RS04725 at 886.842 kb on - strand, within MMJJ_RS04725 at 886.843 kb on + strand, within MMJJ_RS04725 at 886.843 kb on + strand, within MMJJ_RS04725 at 886.843 kb on + strand, within MMJJ_RS04725 at 886.851 kb on - strand, within MMJJ_RS04725 at 886.851 kb on - strand, within MMJJ_RS04725 at 886.851 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.856 kb on + strand, within MMJJ_RS04725 at 886.866 kb on - strand, within MMJJ_RS04725 at 886.867 kb on + strand, within MMJJ_RS04725 at 886.970 kb on - strand, within MMJJ_RS04725 at 887.138 kb on - strand at 887.139 kb on + strand at 887.147 kb on - strand at 887.147 kb on - strand at 887.148 kb on + strand at 887.156 kb on + strand at 887.337 kb on - strand, within MMJJ_RS04730 at 887.697 kb on - strand, within MMJJ_RS04730 at 887.716 kb on - strand, within MMJJ_RS04730 at 887.755 kb on + strand, within MMJJ_RS04730 at 887.830 kb on - strand, within MMJJ_RS04730 at 887.878 kb on + strand, within MMJJ_RS04730 at 888.006 kb on - strand, within MMJJ_RS04730 at 888.258 kb on + strand at 888.355 kb on - strand at 888.355 kb on - strand at 888.355 kb on - strand at 888.366 kb on + strand at 888.396 kb on - strand at 888.397 kb on + strand at 888.442 kb on + strand at 888.442 kb on + strand at 888.469 kb on - strand at 888.507 kb on - strand at 888.511 kb on - strand at 888.782 kb on + strand, within MMJJ_RS04735 at 889.051 kb on + strand, within MMJJ_RS04735
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 886,173 - +4.0 886,187 + -0.2 886,194 + -0.9 886,246 - +0.1 886,247 + +0.3 886,247 + +1.8 886,249 - -0.2 886,249 - -0.7 886,254 - -1.9 886,254 - +0.4 886,255 + +0.0 886,255 + +2.8 886,255 + +3.0 886,258 - +1.3 886,259 + +2.2 886,259 + -0.2 886,281 - -1.6 886,282 + -1.2 886,282 + +0.8 886,295 - +0.5 886,302 - +1.9 886,302 - +0.4 886,302 - -1.3 886,303 + +2.0 886,323 - -1.3 886,338 + MMJJ_RS04725 0.10 -1.6 886,338 + MMJJ_RS04725 0.10 -0.2 886,372 + MMJJ_RS04725 0.14 -2.1 886,395 + MMJJ_RS04725 0.17 +1.7 886,417 + MMJJ_RS04725 0.20 +2.2 886,443 - MMJJ_RS04725 0.23 +3.0 886,446 + MMJJ_RS04725 0.23 +2.9 886,451 - MMJJ_RS04725 0.23 +1.0 886,451 - MMJJ_RS04725 0.23 +3.4 886,452 + MMJJ_RS04725 0.24 +0.3 886,476 - MMJJ_RS04725 0.26 -1.4 886,477 + MMJJ_RS04725 0.27 +1.1 886,494 + MMJJ_RS04725 0.28 +2.6 886,557 - MMJJ_RS04725 0.36 -1.7 886,557 - MMJJ_RS04725 0.36 -2.5 886,557 - MMJJ_RS04725 0.36 -1.3 886,557 - MMJJ_RS04725 0.36 -1.8 886,627 - MMJJ_RS04725 0.44 +2.3 886,659 - MMJJ_RS04725 0.48 +0.4 886,660 + MMJJ_RS04725 0.48 +0.5 886,660 + MMJJ_RS04725 0.48 +1.3 886,689 - MMJJ_RS04725 0.51 +1.5 886,698 - MMJJ_RS04725 0.52 -1.7 886,699 + MMJJ_RS04725 0.52 -2.7 886,704 - MMJJ_RS04725 0.53 +2.0 886,726 + MMJJ_RS04725 0.55 -1.1 886,726 + MMJJ_RS04725 0.55 -1.1 886,728 + MMJJ_RS04725 0.56 -2.0 886,728 + MMJJ_RS04725 0.56 +1.2 886,838 - MMJJ_RS04725 0.68 +6.6 886,838 - MMJJ_RS04725 0.68 -1.1 886,838 - MMJJ_RS04725 0.68 +2.8 886,842 - MMJJ_RS04725 0.69 +2.1 886,842 - MMJJ_RS04725 0.69 +0.2 886,843 + MMJJ_RS04725 0.69 +0.7 886,843 + MMJJ_RS04725 0.69 -1.0 886,843 + MMJJ_RS04725 0.69 +3.8 886,851 - MMJJ_RS04725 0.70 -1.2 886,851 - MMJJ_RS04725 0.70 +2.1 886,851 - MMJJ_RS04725 0.70 +1.1 886,855 - MMJJ_RS04725 0.70 +2.1 886,855 - MMJJ_RS04725 0.70 +2.5 886,855 - MMJJ_RS04725 0.70 +1.6 886,855 - MMJJ_RS04725 0.70 -1.7 886,855 - MMJJ_RS04725 0.70 -2.2 886,856 + MMJJ_RS04725 0.70 -2.9 886,866 - MMJJ_RS04725 0.72 +3.8 886,867 + MMJJ_RS04725 0.72 +1.3 886,970 - MMJJ_RS04725 0.84 -1.4 887,138 - +2.6 887,139 + +0.4 887,147 - +4.2 887,147 - +4.0 887,148 + +1.3 887,156 + +0.8 887,337 - MMJJ_RS04730 0.15 -1.2 887,697 - MMJJ_RS04730 0.46 -1.9 887,716 - MMJJ_RS04730 0.48 +1.4 887,755 + MMJJ_RS04730 0.51 -1.9 887,830 - MMJJ_RS04730 0.58 -0.2 887,878 + MMJJ_RS04730 0.62 -1.2 888,006 - MMJJ_RS04730 0.73 -0.9 888,258 + +0.4 888,355 - +0.4 888,355 - -0.2 888,355 - -1.3 888,366 + -2.8 888,396 - +3.4 888,397 + -1.3 888,442 + +0.3 888,442 + -1.2 888,469 - -0.7 888,507 - +2.4 888,511 - +1.4 888,782 + MMJJ_RS04735 0.38 +1.4 889,051 + MMJJ_RS04735 0.70 -1.2
Or see this region's nucleotide sequence