Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04465 and MMJJ_RS04470 are separated by 108 nucleotides MMJJ_RS04470 and MMJJ_RS04475 are separated by 120 nucleotides MMJJ_RS04475 and MMJJ_RS04480 are separated by 170 nucleotides MMJJ_RS04480 and MMJJ_RS04485 are separated by 143 nucleotides
MMJJ_RS04465: MMJJ_RS04465 - MTAP family purine nucleoside phosphorylase, at 831,868 to 832,629
_RS04465
MMJJ_RS04470: MMJJ_RS04470 - CTP-dependent riboflavin kinase, at 832,738 to 833,121
_RS04470
MMJJ_RS04475: MMJJ_RS04475 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 833,242 to 833,985
_RS04475
MMJJ_RS04480: MMJJ_RS04480 - hypothetical protein, at 834,156 to 834,368
_RS04480
MMJJ_RS04485: MMJJ_RS04485 - riboflavin synthase, at 834,512 to 834,982
_RS04485
Position (kb) 833
834 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 832.243 kb on - strand, within MMJJ_RS04465 at 832.243 kb on - strand, within MMJJ_RS04465 at 832.244 kb on + strand, within MMJJ_RS04465 at 832.256 kb on - strand, within MMJJ_RS04465 at 832.268 kb on + strand, within MMJJ_RS04465 at 832.268 kb on + strand, within MMJJ_RS04465 at 832.305 kb on + strand, within MMJJ_RS04465 at 832.329 kb on - strand, within MMJJ_RS04465 at 832.330 kb on + strand, within MMJJ_RS04465 at 832.372 kb on - strand, within MMJJ_RS04465 at 832.372 kb on - strand, within MMJJ_RS04465 at 832.372 kb on - strand, within MMJJ_RS04465 at 832.372 kb on - strand, within MMJJ_RS04465 at 832.373 kb on + strand, within MMJJ_RS04465 at 832.373 kb on + strand, within MMJJ_RS04465 at 832.384 kb on - strand, within MMJJ_RS04465 at 832.385 kb on + strand, within MMJJ_RS04465 at 832.385 kb on + strand, within MMJJ_RS04465 at 832.402 kb on - strand, within MMJJ_RS04465 at 832.403 kb on + strand, within MMJJ_RS04465 at 832.403 kb on + strand, within MMJJ_RS04465 at 832.412 kb on + strand, within MMJJ_RS04465 at 832.435 kb on - strand, within MMJJ_RS04465 at 832.462 kb on - strand, within MMJJ_RS04465 at 832.462 kb on - strand, within MMJJ_RS04465 at 832.462 kb on - strand, within MMJJ_RS04465 at 832.504 kb on - strand, within MMJJ_RS04465 at 832.504 kb on - strand, within MMJJ_RS04465 at 832.513 kb on - strand, within MMJJ_RS04465 at 832.513 kb on - strand, within MMJJ_RS04465 at 832.513 kb on - strand, within MMJJ_RS04465 at 832.513 kb on - strand, within MMJJ_RS04465 at 832.514 kb on + strand, within MMJJ_RS04465 at 832.535 kb on + strand, within MMJJ_RS04465 at 832.543 kb on + strand, within MMJJ_RS04465 at 832.568 kb on + strand at 832.588 kb on - strand at 832.589 kb on + strand at 832.589 kb on + strand at 832.589 kb on + strand at 832.591 kb on - strand at 832.632 kb on + strand at 832.652 kb on - strand at 832.652 kb on - strand at 832.652 kb on - strand at 832.656 kb on - strand at 832.657 kb on + strand at 832.677 kb on - strand at 832.677 kb on - strand at 832.682 kb on + strand at 832.694 kb on - strand at 832.717 kb on - strand at 832.718 kb on + strand at 832.719 kb on - strand at 832.719 kb on - strand at 832.719 kb on - strand at 832.719 kb on - strand at 832.719 kb on - strand at 832.720 kb on + strand at 832.720 kb on + strand at 832.720 kb on + strand at 832.721 kb on - strand at 833.084 kb on - strand at 833.119 kb on - strand at 833.120 kb on + strand at 833.125 kb on + strand at 833.128 kb on - strand at 833.129 kb on + strand at 833.129 kb on + strand at 833.162 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.193 kb on - strand at 833.193 kb on - strand at 833.193 kb on - strand at 833.193 kb on - strand at 833.193 kb on - strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.200 kb on + strand at 833.227 kb on - strand at 833.228 kb on + strand at 833.230 kb on - strand at 833.230 kb on - strand at 833.231 kb on + strand at 833.247 kb on - strand at 833.286 kb on + strand at 833.416 kb on + strand, within MMJJ_RS04475 at 833.416 kb on + strand, within MMJJ_RS04475 at 833.425 kb on - strand, within MMJJ_RS04475 at 833.511 kb on - strand at 833.565 kb on + strand, within MMJJ_RS04475 at 833.565 kb on + strand, within MMJJ_RS04475 at 833.566 kb on - strand, within MMJJ_RS04475 at 833.649 kb on - strand, within MMJJ_RS04475 at 833.650 kb on + strand, within MMJJ_RS04475 at 833.760 kb on - strand, within MMJJ_RS04475 at 833.841 kb on - strand at 833.929 kb on + strand at 834.068 kb on - strand at 834.069 kb on + strand at 834.070 kb on - strand at 834.070 kb on - strand at 834.070 kb on - strand at 834.071 kb on + strand at 834.071 kb on + strand at 834.104 kb on + strand at 834.135 kb on + strand at 834.135 kb on + strand at 834.139 kb on + strand at 834.155 kb on - strand at 834.164 kb on - strand at 834.179 kb on - strand, within MMJJ_RS04480 at 834.179 kb on - strand, within MMJJ_RS04480 at 834.181 kb on - strand, within MMJJ_RS04480 at 834.276 kb on - strand, within MMJJ_RS04480 at 834.278 kb on - strand, within MMJJ_RS04480 at 834.350 kb on - strand at 834.395 kb on + strand at 834.395 kb on + strand at 834.397 kb on - strand at 834.397 kb on - strand at 834.414 kb on - strand at 834.462 kb on + strand at 834.467 kb on + strand at 834.470 kb on - strand at 834.470 kb on - strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.485 kb on - strand at 834.486 kb on + strand at 834.486 kb on + strand at 834.525 kb on - strand at 834.525 kb on - strand at 834.605 kb on + strand, within MMJJ_RS04485 at 834.613 kb on + strand, within MMJJ_RS04485 at 834.621 kb on + strand, within MMJJ_RS04485 at 834.691 kb on - strand, within MMJJ_RS04485 at 834.691 kb on - strand, within MMJJ_RS04485 at 834.794 kb on + strand, within MMJJ_RS04485 at 834.794 kb on + strand, within MMJJ_RS04485 at 834.843 kb on - strand, within MMJJ_RS04485 at 834.888 kb on - strand, within MMJJ_RS04485 at 834.888 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.904 kb on - strand, within MMJJ_RS04485
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 832,243 - MMJJ_RS04465 0.49 -1.4 832,243 - MMJJ_RS04465 0.49 -1.7 832,244 + MMJJ_RS04465 0.49 -1.9 832,256 - MMJJ_RS04465 0.51 +2.0 832,268 + MMJJ_RS04465 0.52 -0.2 832,268 + MMJJ_RS04465 0.52 -2.1 832,305 + MMJJ_RS04465 0.57 +3.4 832,329 - MMJJ_RS04465 0.60 +0.5 832,330 + MMJJ_RS04465 0.61 +0.7 832,372 - MMJJ_RS04465 0.66 +2.1 832,372 - MMJJ_RS04465 0.66 +0.7 832,372 - MMJJ_RS04465 0.66 +1.6 832,372 - MMJJ_RS04465 0.66 +2.0 832,373 + MMJJ_RS04465 0.66 -1.9 832,373 + MMJJ_RS04465 0.66 -1.7 832,384 - MMJJ_RS04465 0.68 +0.6 832,385 + MMJJ_RS04465 0.68 -1.3 832,385 + MMJJ_RS04465 0.68 -0.3 832,402 - MMJJ_RS04465 0.70 -0.2 832,403 + MMJJ_RS04465 0.70 +2.4 832,403 + MMJJ_RS04465 0.70 +3.0 832,412 + MMJJ_RS04465 0.71 -0.1 832,435 - MMJJ_RS04465 0.74 +2.4 832,462 - MMJJ_RS04465 0.78 -1.7 832,462 - MMJJ_RS04465 0.78 -1.7 832,462 - MMJJ_RS04465 0.78 +0.5 832,504 - MMJJ_RS04465 0.83 -1.9 832,504 - MMJJ_RS04465 0.83 -0.6 832,513 - MMJJ_RS04465 0.85 +0.7 832,513 - MMJJ_RS04465 0.85 -1.2 832,513 - MMJJ_RS04465 0.85 +2.4 832,513 - MMJJ_RS04465 0.85 +1.7 832,514 + MMJJ_RS04465 0.85 +1.4 832,535 + MMJJ_RS04465 0.88 -0.5 832,543 + MMJJ_RS04465 0.89 +0.2 832,568 + -1.0 832,588 - -0.3 832,589 + -0.1 832,589 + -0.3 832,589 + -1.6 832,591 - +0.9 832,632 + +0.8 832,652 - -2.2 832,652 - -0.6 832,652 - +2.0 832,656 - -1.9 832,657 + -1.8 832,677 - -1.9 832,677 - +0.5 832,682 + -2.9 832,694 - +1.5 832,717 - +0.4 832,718 + -1.6 832,719 - +0.4 832,719 - -0.6 832,719 - -1.6 832,719 - -0.6 832,719 - -2.5 832,720 + -0.6 832,720 + +0.4 832,720 + -0.4 832,721 - -1.2 833,084 - -0.9 833,119 - +5.1 833,120 + -0.5 833,125 + +2.1 833,128 - +3.8 833,129 + -0.9 833,129 + -0.1 833,162 - -3.3 833,189 - +0.9 833,189 - -0.8 833,189 - +0.4 833,189 - +3.0 833,189 - -3.0 833,189 - -0.6 833,189 - +0.2 833,189 - +3.1 833,189 - +1.7 833,189 - +1.4 833,190 + +0.9 833,190 + -0.2 833,190 + -1.6 833,190 + +1.0 833,190 + +1.1 833,190 + -1.4 833,190 + +3.3 833,190 + +2.1 833,191 - +1.4 833,191 - -1.6 833,191 - -2.1 833,191 - +2.7 833,191 - +2.1 833,191 - -1.1 833,191 - -1.9 833,192 + +1.5 833,192 + -2.5 833,192 + -0.2 833,192 + +1.1 833,192 + +4.4 833,192 + +1.9 833,192 + -2.1 833,192 + +1.4 833,192 + -1.4 833,192 + -0.9 833,192 + -1.7 833,193 - +0.6 833,193 - -1.0 833,193 - -0.2 833,193 - -1.3 833,193 - -0.4 833,194 + +1.4 833,194 + +0.7 833,194 + +2.8 833,194 + +0.9 833,194 + -0.8 833,194 + +1.2 833,194 + +2.3 833,194 + -1.1 833,194 + +1.7 833,200 + -2.0 833,227 - -1.8 833,228 + -1.5 833,230 - -2.3 833,230 - -0.6 833,231 + -2.3 833,247 - -3.4 833,286 + -2.6 833,416 + MMJJ_RS04475 0.23 -2.6 833,416 + MMJJ_RS04475 0.23 -1.8 833,425 - MMJJ_RS04475 0.25 -1.8 833,511 - +0.4 833,565 + MMJJ_RS04475 0.43 -0.2 833,565 + MMJJ_RS04475 0.43 -0.9 833,566 - MMJJ_RS04475 0.44 -0.8 833,649 - MMJJ_RS04475 0.55 +0.4 833,650 + MMJJ_RS04475 0.55 -2.1 833,760 - MMJJ_RS04475 0.70 -0.9 833,841 - +1.4 833,929 + -2.6 834,068 - -2.8 834,069 + +1.1 834,070 - -1.7 834,070 - -1.1 834,070 - -1.2 834,071 + -2.5 834,071 + +0.7 834,104 + +0.7 834,135 + +1.1 834,135 + +2.3 834,139 + +0.3 834,155 - +1.4 834,164 - -0.2 834,179 - MMJJ_RS04480 0.11 -0.6 834,179 - MMJJ_RS04480 0.11 -1.5 834,181 - MMJJ_RS04480 0.12 -1.6 834,276 - MMJJ_RS04480 0.56 +2.1 834,278 - MMJJ_RS04480 0.57 +2.5 834,350 - -1.1 834,395 + -1.8 834,395 + +0.2 834,397 - -2.3 834,397 - +0.5 834,414 - +0.9 834,462 + -2.4 834,467 + -2.2 834,470 - -0.9 834,470 - -2.5 834,476 + +1.0 834,476 + -0.7 834,476 + -0.6 834,476 + -2.1 834,476 + -1.9 834,485 - -0.2 834,486 + -1.1 834,486 + -1.6 834,525 - +1.4 834,525 - -0.2 834,605 + MMJJ_RS04485 0.20 -3.3 834,613 + MMJJ_RS04485 0.21 -2.8 834,621 + MMJJ_RS04485 0.23 -1.5 834,691 - MMJJ_RS04485 0.38 -0.8 834,691 - MMJJ_RS04485 0.38 -2.5 834,794 + MMJJ_RS04485 0.60 -1.2 834,794 + MMJJ_RS04485 0.60 -0.6 834,843 - MMJJ_RS04485 0.70 -3.0 834,888 - MMJJ_RS04485 0.80 +0.4 834,888 - MMJJ_RS04485 0.80 -0.9 834,893 - MMJJ_RS04485 0.81 -0.9 834,893 - MMJJ_RS04485 0.81 -1.4 834,893 - MMJJ_RS04485 0.81 -1.2 834,893 - MMJJ_RS04485 0.81 -0.4 834,894 + MMJJ_RS04485 0.81 -0.9 834,894 + MMJJ_RS04485 0.81 -1.6 834,894 + MMJJ_RS04485 0.81 -1.2 834,894 + MMJJ_RS04485 0.81 -3.1 834,894 + MMJJ_RS04485 0.81 -1.9 834,904 - MMJJ_RS04485 0.83 -2.5
Or see this region's nucleotide sequence