Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04435 and MMJJ_RS04440 are separated by 5 nucleotides MMJJ_RS04440 and MMJJ_RS04445 are separated by 334 nucleotides MMJJ_RS04445 and MMJJ_RS04450 are separated by 84 nucleotides MMJJ_RS04450 and MMJJ_RS04455 are separated by 89 nucleotides
MMJJ_RS04435: MMJJ_RS04435 - phosphotransferase, at 827,051 to 827,977
_RS04435
MMJJ_RS04440: MMJJ_RS04440 - hypothetical protein, at 827,983 to 828,360
_RS04440
MMJJ_RS04445: MMJJ_RS04445 - tRNA-Leu, at 828,695 to 828,779
_RS04445
MMJJ_RS04450: MMJJ_RS04450 - diphthamide biosynthesis enzyme Dph2, at 828,864 to 829,793
_RS04450
MMJJ_RS04455: MMJJ_RS04455 - phosphomethylpyrimidine synthase, at 829,883 to 831,163
_RS04455
Position (kb) 828
829
830 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 827.887 kb on - strand at 827.900 kb on - strand at 827.900 kb on - strand at 827.928 kb on + strand at 827.929 kb on - strand at 827.929 kb on - strand at 827.929 kb on - strand at 827.968 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.980 kb on - strand at 827.981 kb on + strand at 827.981 kb on + strand at 827.985 kb on + strand at 827.985 kb on + strand at 827.986 kb on - strand at 827.987 kb on + strand at 827.992 kb on + strand at 828.010 kb on + strand at 828.222 kb on - strand, within MMJJ_RS04440 at 828.223 kb on + strand, within MMJJ_RS04440 at 828.223 kb on + strand, within MMJJ_RS04440 at 828.225 kb on - strand, within MMJJ_RS04440 at 828.225 kb on - strand, within MMJJ_RS04440 at 828.226 kb on + strand, within MMJJ_RS04440 at 828.226 kb on + strand, within MMJJ_RS04440 at 828.226 kb on + strand, within MMJJ_RS04440 at 828.229 kb on - strand, within MMJJ_RS04440 at 828.230 kb on + strand, within MMJJ_RS04440 at 828.248 kb on - strand, within MMJJ_RS04440 at 828.289 kb on - strand, within MMJJ_RS04440 at 828.306 kb on + strand, within MMJJ_RS04440 at 828.349 kb on - strand at 828.350 kb on + strand at 828.376 kb on - strand at 828.389 kb on - strand at 828.393 kb on + strand at 828.487 kb on + strand at 828.496 kb on + strand at 828.535 kb on - strand at 828.866 kb on + strand at 828.868 kb on - strand at 828.869 kb on + strand at 828.922 kb on - strand at 829.004 kb on - strand, within MMJJ_RS04450 at 829.009 kb on - strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.010 kb on + strand, within MMJJ_RS04450 at 829.030 kb on + strand, within MMJJ_RS04450 at 829.044 kb on - strand, within MMJJ_RS04450 at 829.044 kb on - strand, within MMJJ_RS04450 at 829.045 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.068 kb on + strand, within MMJJ_RS04450 at 829.070 kb on + strand, within MMJJ_RS04450 at 829.079 kb on - strand, within MMJJ_RS04450 at 829.090 kb on + strand, within MMJJ_RS04450 at 829.180 kb on + strand, within MMJJ_RS04450 at 829.182 kb on - strand, within MMJJ_RS04450 at 829.183 kb on + strand, within MMJJ_RS04450 at 829.259 kb on - strand, within MMJJ_RS04450 at 829.260 kb on + strand, within MMJJ_RS04450 at 829.290 kb on - strand, within MMJJ_RS04450 at 829.296 kb on + strand, within MMJJ_RS04450 at 829.297 kb on - strand, within MMJJ_RS04450 at 829.297 kb on - strand, within MMJJ_RS04450 at 829.298 kb on + strand, within MMJJ_RS04450 at 829.307 kb on - strand, within MMJJ_RS04450 at 829.307 kb on - strand at 829.308 kb on + strand, within MMJJ_RS04450 at 829.308 kb on + strand, within MMJJ_RS04450 at 829.384 kb on + strand, within MMJJ_RS04450 at 829.572 kb on + strand, within MMJJ_RS04450 at 829.765 kb on + strand at 829.772 kb on - strand at 829.773 kb on + strand at 829.794 kb on + strand at 829.794 kb on + strand at 829.796 kb on - strand at 829.797 kb on + strand at 829.813 kb on + strand at 829.816 kb on - strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.828 kb on + strand at 829.832 kb on + strand at 829.832 kb on + strand at 829.834 kb on - strand at 829.834 kb on - strand at 829.835 kb on + strand at 829.838 kb on + strand at 829.849 kb on - strand at 829.869 kb on + strand at 829.871 kb on + strand at 830.174 kb on + strand at 830.249 kb on - strand, within MMJJ_RS04455
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 827,887 - +0.2 827,900 - -3.5 827,900 - +2.7 827,928 + -0.9 827,929 - -0.0 827,929 - +2.9 827,929 - -2.2 827,968 - +0.7 827,980 - +3.0 827,980 - -0.4 827,980 - -2.2 827,980 - -1.8 827,980 - +0.9 827,980 - -2.6 827,980 - -1.2 827,981 + +2.7 827,981 + +1.7 827,985 + -0.9 827,985 + -0.1 827,986 - -0.5 827,987 + +2.8 827,992 + -0.9 828,010 + -0.6 828,222 - MMJJ_RS04440 0.63 -0.8 828,223 + MMJJ_RS04440 0.63 +2.8 828,223 + MMJJ_RS04440 0.63 -3.4 828,225 - MMJJ_RS04440 0.64 -1.3 828,225 - MMJJ_RS04440 0.64 -0.9 828,226 + MMJJ_RS04440 0.64 +0.6 828,226 + MMJJ_RS04440 0.64 -0.6 828,226 + MMJJ_RS04440 0.64 -1.9 828,229 - MMJJ_RS04440 0.65 +0.8 828,230 + MMJJ_RS04440 0.65 -0.8 828,248 - MMJJ_RS04440 0.70 -0.7 828,289 - MMJJ_RS04440 0.81 -2.1 828,306 + MMJJ_RS04440 0.85 -0.4 828,349 - -0.6 828,350 + +2.2 828,376 - +2.6 828,389 - +0.9 828,393 + -1.2 828,487 + +1.2 828,496 + +3.3 828,535 - +0.9 828,866 + -1.2 828,868 - +1.7 828,869 + +0.8 828,922 - +0.5 829,004 - MMJJ_RS04450 0.15 -1.3 829,009 - MMJJ_RS04450 0.16 +3.4 829,010 + MMJJ_RS04450 0.16 -0.4 829,010 + MMJJ_RS04450 0.16 -0.9 829,010 + MMJJ_RS04450 0.16 +2.0 829,030 + MMJJ_RS04450 0.18 +2.0 829,044 - MMJJ_RS04450 0.19 -0.9 829,044 - MMJJ_RS04450 0.19 +1.8 829,045 + MMJJ_RS04450 0.19 +1.1 829,068 + MMJJ_RS04450 0.22 +2.3 829,068 + MMJJ_RS04450 0.22 +0.9 829,068 + MMJJ_RS04450 0.22 +0.2 829,070 + MMJJ_RS04450 0.22 +1.4 829,079 - MMJJ_RS04450 0.23 +2.3 829,090 + MMJJ_RS04450 0.24 -3.1 829,180 + MMJJ_RS04450 0.34 +2.2 829,182 - MMJJ_RS04450 0.34 +2.4 829,183 + MMJJ_RS04450 0.34 -2.1 829,259 - MMJJ_RS04450 0.42 +1.4 829,260 + MMJJ_RS04450 0.43 +0.6 829,290 - MMJJ_RS04450 0.46 -1.2 829,296 + MMJJ_RS04450 0.46 -1.6 829,297 - MMJJ_RS04450 0.47 -0.4 829,297 - MMJJ_RS04450 0.47 +2.6 829,298 + MMJJ_RS04450 0.47 -0.9 829,307 - MMJJ_RS04450 0.48 +2.4 829,307 - -0.2 829,308 + MMJJ_RS04450 0.48 +3.6 829,308 + MMJJ_RS04450 0.48 -1.9 829,384 + MMJJ_RS04450 0.56 +1.3 829,572 + MMJJ_RS04450 0.76 +2.4 829,765 + -1.4 829,772 - +3.2 829,773 + -1.8 829,794 + -2.7 829,794 + -1.2 829,796 - -2.3 829,797 + +2.1 829,813 + +3.5 829,816 - -0.2 829,828 + +0.1 829,828 + +1.1 829,828 + -0.1 829,828 + -1.8 829,828 + +1.1 829,832 + +0.8 829,832 + -1.6 829,834 - -1.2 829,834 - -1.6 829,835 + -0.2 829,838 + -0.6 829,849 - +0.4 829,869 + +0.4 829,871 + -1.6 830,174 + +1.4 830,249 - MMJJ_RS04455 0.29 -0.2
Or see this region's nucleotide sequence