Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03650 and MMJJ_RS03655 are separated by 9 nucleotides MMJJ_RS03655 and MMJJ_RS03660 are separated by 0 nucleotides MMJJ_RS03660 and MMJJ_RS03665 are separated by 132 nucleotides
MMJJ_RS03650: MMJJ_RS03650 - class I SAM-dependent methyltransferase family protein, at 680,985 to 682,013
_RS03650
MMJJ_RS03655: MMJJ_RS03655 - replication factor C large subunit, at 682,023 to 683,483
_RS03655
MMJJ_RS03660: MMJJ_RS03660 - DUF447 family protein, at 683,484 to 684,029
_RS03660
MMJJ_RS03665: MMJJ_RS03665 - shikimate kinase, at 684,162 to 685,013
_RS03665
Position (kb) 682
683
684 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 681.055 kb on - strand at 681.056 kb on + strand at 681.059 kb on - strand at 681.062 kb on - strand at 681.099 kb on - strand, within MMJJ_RS03650 at 681.286 kb on + strand, within MMJJ_RS03650 at 681.476 kb on - strand, within MMJJ_RS03650 at 681.477 kb on + strand, within MMJJ_RS03650 at 681.483 kb on - strand, within MMJJ_RS03650 at 681.483 kb on - strand, within MMJJ_RS03650 at 681.484 kb on + strand, within MMJJ_RS03650 at 681.484 kb on + strand, within MMJJ_RS03650 at 681.528 kb on - strand, within MMJJ_RS03650 at 681.577 kb on - strand, within MMJJ_RS03650 at 681.578 kb on + strand, within MMJJ_RS03650 at 681.595 kb on - strand, within MMJJ_RS03650 at 681.595 kb on - strand, within MMJJ_RS03650 at 681.596 kb on + strand, within MMJJ_RS03650 at 681.629 kb on - strand, within MMJJ_RS03650 at 681.630 kb on + strand, within MMJJ_RS03650 at 681.712 kb on - strand, within MMJJ_RS03650 at 681.713 kb on + strand, within MMJJ_RS03650 at 681.788 kb on + strand, within MMJJ_RS03650 at 681.865 kb on + strand, within MMJJ_RS03650 at 681.868 kb on + strand, within MMJJ_RS03650 at 681.958 kb on + strand at 681.976 kb on - strand at 681.976 kb on - strand at 681.976 kb on - strand at 681.977 kb on + strand at 681.977 kb on + strand at 681.983 kb on + strand at 681.997 kb on - strand at 681.998 kb on + strand at 682.001 kb on + strand at 682.012 kb on + strand at 682.017 kb on + strand at 682.161 kb on + strand at 682.186 kb on + strand, within MMJJ_RS03655 at 682.186 kb on + strand, within MMJJ_RS03655 at 682.242 kb on + strand, within MMJJ_RS03655 at 682.260 kb on - strand, within MMJJ_RS03655 at 682.311 kb on + strand, within MMJJ_RS03655 at 682.512 kb on + strand, within MMJJ_RS03655 at 682.732 kb on + strand, within MMJJ_RS03655 at 683.067 kb on - strand at 683.297 kb on + strand at 683.485 kb on - strand at 683.494 kb on + strand at 683.608 kb on + strand, within MMJJ_RS03660 at 683.647 kb on - strand, within MMJJ_RS03660 at 683.712 kb on + strand, within MMJJ_RS03660 at 683.869 kb on - strand, within MMJJ_RS03660 at 683.882 kb on - strand, within MMJJ_RS03660 at 683.887 kb on + strand, within MMJJ_RS03660 at 683.893 kb on - strand, within MMJJ_RS03660 at 683.893 kb on - strand, within MMJJ_RS03660 at 683.894 kb on + strand at 683.938 kb on - strand, within MMJJ_RS03660 at 684.030 kb on + strand at 684.030 kb on + strand at 684.043 kb on + strand at 684.056 kb on - strand at 684.056 kb on - strand at 684.057 kb on + strand at 684.057 kb on + strand at 684.060 kb on + strand at 684.070 kb on + strand at 684.070 kb on + strand at 684.071 kb on - strand at 684.071 kb on - strand at 684.071 kb on - strand at 684.074 kb on - strand at 684.075 kb on + strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.078 kb on + strand at 684.078 kb on + strand at 684.079 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.083 kb on + strand at 684.085 kb on - strand at 684.085 kb on - strand at 684.085 kb on - strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.098 kb on - strand at 684.099 kb on + strand at 684.099 kb on + strand at 684.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 681,055 - +0.7 681,056 + -1.4 681,059 - -0.6 681,062 - +0.4 681,099 - MMJJ_RS03650 0.11 +0.8 681,286 + MMJJ_RS03650 0.29 +3.0 681,476 - MMJJ_RS03650 0.48 +2.2 681,477 + MMJJ_RS03650 0.48 +1.2 681,483 - MMJJ_RS03650 0.48 -0.6 681,483 - MMJJ_RS03650 0.48 +2.8 681,484 + MMJJ_RS03650 0.48 +0.8 681,484 + MMJJ_RS03650 0.48 -0.2 681,528 - MMJJ_RS03650 0.53 +2.0 681,577 - MMJJ_RS03650 0.58 +1.6 681,578 + MMJJ_RS03650 0.58 +0.8 681,595 - MMJJ_RS03650 0.59 +0.6 681,595 - MMJJ_RS03650 0.59 -0.2 681,596 + MMJJ_RS03650 0.59 -1.9 681,629 - MMJJ_RS03650 0.63 +0.4 681,630 + MMJJ_RS03650 0.63 +3.4 681,712 - MMJJ_RS03650 0.71 +1.1 681,713 + MMJJ_RS03650 0.71 +4.5 681,788 + MMJJ_RS03650 0.78 +1.9 681,865 + MMJJ_RS03650 0.86 +2.1 681,868 + MMJJ_RS03650 0.86 +1.2 681,958 + +0.4 681,976 - +3.2 681,976 - -0.2 681,976 - -1.8 681,977 + +0.4 681,977 + -2.4 681,983 + +0.4 681,997 - -1.8 681,998 + -2.2 682,001 + -1.8 682,012 + -1.9 682,017 + -1.3 682,161 + +1.0 682,186 + MMJJ_RS03655 0.11 -3.8 682,186 + MMJJ_RS03655 0.11 -0.0 682,242 + MMJJ_RS03655 0.15 -1.8 682,260 - MMJJ_RS03655 0.16 -1.0 682,311 + MMJJ_RS03655 0.20 -1.2 682,512 + MMJJ_RS03655 0.33 +0.7 682,732 + MMJJ_RS03655 0.49 -1.2 683,067 - +0.4 683,297 + -0.6 683,485 - +1.4 683,494 + -1.3 683,608 + MMJJ_RS03660 0.23 -0.2 683,647 - MMJJ_RS03660 0.30 -2.2 683,712 + MMJJ_RS03660 0.42 -0.9 683,869 - MMJJ_RS03660 0.71 -0.6 683,882 - MMJJ_RS03660 0.73 -0.2 683,887 + MMJJ_RS03660 0.74 -0.2 683,893 - MMJJ_RS03660 0.75 -0.2 683,893 - MMJJ_RS03660 0.75 +1.4 683,894 + +1.4 683,938 - MMJJ_RS03660 0.83 -0.2 684,030 + -0.9 684,030 + +0.4 684,043 + -1.7 684,056 - +1.4 684,056 - +1.4 684,057 + -0.9 684,057 + -2.4 684,060 + -2.2 684,070 + -2.1 684,070 + -0.8 684,071 - -2.9 684,071 - -2.7 684,071 - -1.9 684,074 - -0.6 684,075 + -2.1 684,077 - -2.1 684,077 - +0.8 684,077 - -2.7 684,077 - -1.3 684,078 + -0.7 684,078 + -2.8 684,079 - -1.1 684,082 - -1.1 684,082 - -2.5 684,082 - +1.5 684,082 - +2.2 684,083 + -1.1 684,085 - -1.1 684,085 - -3.4 684,085 - -0.8 684,086 + +0.2 684,086 + -2.9 684,086 + -0.9 684,086 + -1.6 684,086 + +1.2 684,086 + +0.1 684,098 - -0.1 684,099 + +2.0 684,099 + +2.9 684,103 - -0.6
Or see this region's nucleotide sequence