Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03580 and MMJJ_RS03585 are separated by 29 nucleotides MMJJ_RS03585 and MMJJ_RS03590 are separated by 16 nucleotides MMJJ_RS03590 and MMJJ_RS03595 are separated by 47 nucleotides MMJJ_RS03595 and MMJJ_RS03600 are separated by 70 nucleotides MMJJ_RS03600 and MMJJ_RS03605 are separated by 161 nucleotides
MMJJ_RS03580: MMJJ_RS03580 - TIGR00303 family protein, at 668,823 to 669,875
_RS03580
MMJJ_RS03585: MMJJ_RS03585 - methanogenesis marker 6 protein, at 669,905 to 670,351
_RS03585
MMJJ_RS03590: MMJJ_RS03590 - restriction endonuclease, at 670,368 to 670,775
_RS03590
MMJJ_RS03595: MMJJ_RS03595 - hypothetical protein, at 670,823 to 671,236
_RS03595
MMJJ_RS03600: MMJJ_RS03600 - TrmJ/YjtD family RNA methyltransferase, at 671,307 to 672,011
_RS03600
MMJJ_RS03605: MMJJ_RS03605 - TIGR00297 family protein, at 672,173 to 672,886
_RS03605
Position (kb) 670
671
672 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 669.829 kb on + strand at 669.850 kb on - strand at 669.851 kb on + strand at 669.968 kb on - strand, within MMJJ_RS03585 at 670.291 kb on - strand, within MMJJ_RS03585 at 670.336 kb on + strand at 670.336 kb on + strand at 670.336 kb on + strand at 670.349 kb on - strand at 670.350 kb on + strand at 670.376 kb on - strand at 670.377 kb on + strand at 670.381 kb on + strand at 670.394 kb on - strand at 670.394 kb on - strand at 670.477 kb on - strand, within MMJJ_RS03590 at 670.508 kb on - strand, within MMJJ_RS03590 at 670.545 kb on - strand, within MMJJ_RS03590 at 670.546 kb on + strand, within MMJJ_RS03590 at 670.626 kb on + strand, within MMJJ_RS03590 at 670.639 kb on - strand, within MMJJ_RS03590 at 670.639 kb on - strand, within MMJJ_RS03590 at 670.644 kb on + strand, within MMJJ_RS03590 at 670.645 kb on - strand, within MMJJ_RS03590 at 670.646 kb on + strand, within MMJJ_RS03590 at 670.745 kb on - strand at 670.773 kb on - strand at 670.773 kb on - strand at 670.774 kb on + strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.777 kb on + strand at 670.778 kb on - strand at 670.778 kb on - strand at 670.778 kb on - strand at 670.779 kb on + strand at 670.779 kb on + strand at 670.792 kb on + strand at 670.839 kb on + strand at 670.866 kb on + strand, within MMJJ_RS03595 at 670.866 kb on + strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.870 kb on + strand, within MMJJ_RS03595 at 670.870 kb on + strand, within MMJJ_RS03595 at 670.889 kb on - strand, within MMJJ_RS03595 at 670.890 kb on + strand, within MMJJ_RS03595 at 670.968 kb on + strand, within MMJJ_RS03595 at 670.984 kb on - strand, within MMJJ_RS03595 at 671.019 kb on + strand, within MMJJ_RS03595 at 671.166 kb on + strand, within MMJJ_RS03595 at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.230 kb on - strand at 671.230 kb on - strand at 671.230 kb on - strand at 671.257 kb on - strand at 671.257 kb on - strand at 671.258 kb on + strand at 671.261 kb on + strand at 671.266 kb on - strand at 671.270 kb on + strand at 671.276 kb on + strand at 671.276 kb on + strand at 671.309 kb on + strand at 671.321 kb on - strand at 671.322 kb on + strand at 671.328 kb on + strand at 671.337 kb on - strand at 671.338 kb on + strand at 671.341 kb on + strand at 671.391 kb on + strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.407 kb on + strand, within MMJJ_RS03600 at 671.407 kb on + strand, within MMJJ_RS03600 at 671.417 kb on - strand, within MMJJ_RS03600 at 671.556 kb on - strand, within MMJJ_RS03600 at 671.640 kb on - strand, within MMJJ_RS03600 at 671.640 kb on - strand, within MMJJ_RS03600 at 671.641 kb on + strand, within MMJJ_RS03600 at 671.682 kb on - strand, within MMJJ_RS03600 at 671.735 kb on - strand, within MMJJ_RS03600 at 671.751 kb on - strand, within MMJJ_RS03600 at 671.792 kb on - strand, within MMJJ_RS03600 at 671.793 kb on + strand, within MMJJ_RS03600 at 671.852 kb on - strand, within MMJJ_RS03600 at 671.876 kb on - strand, within MMJJ_RS03600 at 671.876 kb on - strand, within MMJJ_RS03600 at 671.877 kb on + strand, within MMJJ_RS03600 at 671.877 kb on + strand, within MMJJ_RS03600 at 671.878 kb on - strand, within MMJJ_RS03600 at 671.878 kb on - strand, within MMJJ_RS03600 at 671.879 kb on + strand, within MMJJ_RS03600 at 671.879 kb on + strand, within MMJJ_RS03600 at 671.879 kb on + strand, within MMJJ_RS03600 at 671.879 kb on + strand, within MMJJ_RS03600 at 671.913 kb on - strand, within MMJJ_RS03600 at 671.978 kb on + strand at 671.996 kb on - strand at 671.996 kb on - strand at 671.997 kb on + strand at 672.057 kb on + strand at 672.062 kb on - strand at 672.062 kb on - strand at 672.062 kb on - strand at 672.063 kb on + strand at 672.067 kb on - strand at 672.067 kb on - strand at 672.067 kb on - strand at 672.068 kb on + strand at 672.079 kb on + strand at 672.079 kb on + strand at 672.082 kb on - strand at 672.082 kb on - strand at 672.083 kb on + strand at 672.085 kb on - strand at 672.089 kb on - strand at 672.090 kb on + strand at 672.121 kb on + strand at 672.169 kb on - strand at 672.174 kb on - strand at 672.174 kb on - strand at 672.180 kb on + strand at 672.180 kb on + strand at 672.189 kb on - strand at 672.190 kb on + strand at 672.218 kb on - strand at 672.219 kb on + strand at 672.219 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 669,829 + +0.4 669,850 - -0.6 669,851 + +4.4 669,968 - MMJJ_RS03585 0.14 -0.2 670,291 - MMJJ_RS03585 0.86 -1.8 670,336 + -0.9 670,336 + +1.9 670,336 + +3.1 670,349 - +4.4 670,350 + -1.2 670,376 - -0.0 670,377 + -1.4 670,381 + -3.4 670,394 - -0.1 670,394 - -3.6 670,477 - MMJJ_RS03590 0.27 +1.2 670,508 - MMJJ_RS03590 0.34 -0.8 670,545 - MMJJ_RS03590 0.43 +1.8 670,546 + MMJJ_RS03590 0.44 -0.4 670,626 + MMJJ_RS03590 0.63 -1.4 670,639 - MMJJ_RS03590 0.66 -0.1 670,639 - MMJJ_RS03590 0.66 +0.0 670,644 + MMJJ_RS03590 0.68 -0.9 670,645 - MMJJ_RS03590 0.68 +1.5 670,646 + MMJJ_RS03590 0.68 -1.0 670,745 - -0.2 670,773 - +0.4 670,773 - +0.1 670,774 + -2.8 670,776 - -0.3 670,776 - -1.9 670,776 - +1.3 670,776 - -0.5 670,777 + -2.5 670,778 - -0.6 670,778 - -1.1 670,778 - +1.5 670,779 + +2.4 670,779 + -4.5 670,792 + +2.1 670,839 + +1.6 670,866 + MMJJ_RS03595 0.10 -0.9 670,866 + MMJJ_RS03595 0.10 +2.4 670,869 - MMJJ_RS03595 0.11 +0.0 670,869 - MMJJ_RS03595 0.11 +1.2 670,869 - MMJJ_RS03595 0.11 -0.9 670,870 + MMJJ_RS03595 0.11 +1.7 670,870 + MMJJ_RS03595 0.11 +0.9 670,889 - MMJJ_RS03595 0.16 +2.1 670,890 + MMJJ_RS03595 0.16 -1.2 670,968 + MMJJ_RS03595 0.35 +1.3 670,984 - MMJJ_RS03595 0.39 +1.2 671,019 + MMJJ_RS03595 0.47 +0.5 671,166 + MMJJ_RS03595 0.83 +1.4 671,229 + -0.5 671,229 + +0.3 671,229 + +2.8 671,229 + +0.1 671,229 + -1.4 671,230 - +0.7 671,230 - -2.0 671,230 - -0.5 671,257 - +0.9 671,257 - +3.2 671,258 + -0.4 671,261 + +0.8 671,266 - -0.4 671,270 + +2.9 671,276 + +1.7 671,276 + +2.7 671,309 + +1.6 671,321 - -1.8 671,322 + -3.5 671,328 + -2.8 671,337 - +0.6 671,338 + -0.8 671,341 + -0.2 671,391 + MMJJ_RS03600 0.12 -2.5 671,406 - MMJJ_RS03600 0.14 -0.3 671,406 - MMJJ_RS03600 0.14 -1.4 671,406 - MMJJ_RS03600 0.14 +2.4 671,406 - MMJJ_RS03600 0.14 +0.9 671,406 - MMJJ_RS03600 0.14 +1.4 671,406 - MMJJ_RS03600 0.14 +1.2 671,406 - MMJJ_RS03600 0.14 -2.0 671,407 + MMJJ_RS03600 0.14 -0.0 671,407 + MMJJ_RS03600 0.14 -2.5 671,417 - MMJJ_RS03600 0.16 +1.7 671,556 - MMJJ_RS03600 0.35 +2.3 671,640 - MMJJ_RS03600 0.47 -0.9 671,640 - MMJJ_RS03600 0.47 -1.4 671,641 + MMJJ_RS03600 0.47 +0.8 671,682 - MMJJ_RS03600 0.53 +2.4 671,735 - MMJJ_RS03600 0.61 +2.1 671,751 - MMJJ_RS03600 0.63 -1.3 671,792 - MMJJ_RS03600 0.69 -0.5 671,793 + MMJJ_RS03600 0.69 +0.6 671,852 - MMJJ_RS03600 0.77 -0.2 671,876 - MMJJ_RS03600 0.81 -1.7 671,876 - MMJJ_RS03600 0.81 -1.4 671,877 + MMJJ_RS03600 0.81 +1.6 671,877 + MMJJ_RS03600 0.81 +0.1 671,878 - MMJJ_RS03600 0.81 -2.0 671,878 - MMJJ_RS03600 0.81 +0.2 671,879 + MMJJ_RS03600 0.81 +0.5 671,879 + MMJJ_RS03600 0.81 -1.4 671,879 + MMJJ_RS03600 0.81 -0.9 671,879 + MMJJ_RS03600 0.81 +1.5 671,913 - MMJJ_RS03600 0.86 +0.1 671,978 + -2.7 671,996 - -2.5 671,996 - -1.9 671,997 + -0.4 672,057 + +0.4 672,062 - +1.8 672,062 - +1.1 672,062 - -0.4 672,063 + +3.9 672,067 - +0.1 672,067 - +2.5 672,067 - -2.7 672,068 + -2.7 672,079 + -2.7 672,079 + +4.6 672,082 - +0.8 672,082 - -1.4 672,083 + +4.0 672,085 - +1.6 672,089 - -1.3 672,090 + -0.2 672,121 + -2.2 672,169 - -1.2 672,174 - -0.9 672,174 - -1.2 672,180 + -0.2 672,180 + -0.6 672,189 - -0.8 672,190 + -1.4 672,218 - +1.5 672,219 + +2.6 672,219 + +5.5
Or see this region's nucleotide sequence