Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03290 and MMJJ_RS03295 are separated by 28 nucleotides MMJJ_RS03295 and MMJJ_RS03300 are separated by 5 nucleotides
MMJJ_RS03290: MMJJ_RS03290 - amidohydrolase, at 622,964 to 624,244
_RS03290
MMJJ_RS03295: MMJJ_RS03295 - PHP domain-containing protein, at 624,273 to 624,917
_RS03295
MMJJ_RS03300: MMJJ_RS03300 - RNA ligase, at 624,923 to 626,107
_RS03300
Position (kb) 624
625
626
627 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 623.940 kb on - strand, within MMJJ_RS03290 at 623.940 kb on - strand, within MMJJ_RS03290 at 623.944 kb on - strand, within MMJJ_RS03290 at 623.945 kb on + strand, within MMJJ_RS03290 at 624.010 kb on - strand, within MMJJ_RS03290 at 624.038 kb on + strand, within MMJJ_RS03290 at 624.040 kb on - strand, within MMJJ_RS03290 at 624.070 kb on + strand, within MMJJ_RS03290 at 624.082 kb on - strand, within MMJJ_RS03290 at 624.085 kb on - strand, within MMJJ_RS03290 at 624.087 kb on - strand, within MMJJ_RS03290 at 624.102 kb on - strand, within MMJJ_RS03290 at 624.102 kb on - strand, within MMJJ_RS03290 at 624.102 kb on - strand, within MMJJ_RS03290 at 624.103 kb on + strand, within MMJJ_RS03290 at 624.103 kb on + strand, within MMJJ_RS03290 at 624.103 kb on + strand, within MMJJ_RS03290 at 624.135 kb on - strand at 624.138 kb on - strand at 624.151 kb on - strand at 624.151 kb on - strand at 624.151 kb on - strand at 624.152 kb on + strand at 624.195 kb on - strand at 624.195 kb on - strand at 624.195 kb on - strand at 624.196 kb on + strand at 624.196 kb on + strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.200 kb on + strand at 624.202 kb on - strand at 624.214 kb on - strand at 624.214 kb on - strand at 624.214 kb on - strand at 624.215 kb on + strand at 624.295 kb on - strand at 624.295 kb on - strand at 624.295 kb on - strand at 624.295 kb on - strand at 624.296 kb on + strand at 624.419 kb on + strand, within MMJJ_RS03295 at 624.496 kb on - strand, within MMJJ_RS03295 at 624.540 kb on - strand, within MMJJ_RS03295 at 624.541 kb on + strand, within MMJJ_RS03295 at 624.584 kb on - strand, within MMJJ_RS03295 at 624.584 kb on - strand, within MMJJ_RS03295 at 624.616 kb on - strand, within MMJJ_RS03295 at 624.617 kb on + strand, within MMJJ_RS03295 at 624.631 kb on - strand, within MMJJ_RS03295 at 624.631 kb on - strand, within MMJJ_RS03295 at 624.631 kb on - strand, within MMJJ_RS03295 at 624.632 kb on + strand, within MMJJ_RS03295 at 624.632 kb on + strand, within MMJJ_RS03295 at 624.632 kb on + strand, within MMJJ_RS03295 at 624.640 kb on - strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.641 kb on + strand, within MMJJ_RS03295 at 624.642 kb on - strand, within MMJJ_RS03295 at 624.642 kb on - strand, within MMJJ_RS03295 at 624.643 kb on + strand, within MMJJ_RS03295 at 624.643 kb on + strand, within MMJJ_RS03295 at 624.644 kb on - strand, within MMJJ_RS03295 at 624.684 kb on - strand, within MMJJ_RS03295 at 624.685 kb on + strand, within MMJJ_RS03295 at 624.701 kb on - strand, within MMJJ_RS03295 at 624.702 kb on + strand, within MMJJ_RS03295 at 624.752 kb on + strand, within MMJJ_RS03295 at 624.762 kb on - strand, within MMJJ_RS03295 at 624.787 kb on - strand, within MMJJ_RS03295 at 624.788 kb on + strand, within MMJJ_RS03295 at 624.792 kb on + strand, within MMJJ_RS03295 at 624.844 kb on + strand, within MMJJ_RS03295 at 624.844 kb on + strand, within MMJJ_RS03295 at 624.877 kb on - strand at 624.877 kb on - strand at 624.877 kb on - strand at 624.877 kb on - strand at 624.877 kb on - strand at 624.878 kb on + strand at 624.878 kb on + strand at 624.878 kb on + strand at 624.878 kb on + strand at 624.878 kb on + strand at 624.884 kb on - strand at 624.885 kb on + strand at 624.910 kb on - strand at 624.936 kb on + strand at 624.936 kb on + strand at 624.949 kb on - strand at 624.975 kb on - strand at 624.976 kb on + strand at 625.046 kb on + strand, within MMJJ_RS03300 at 625.144 kb on - strand, within MMJJ_RS03300 at 625.147 kb on - strand, within MMJJ_RS03300 at 625.203 kb on + strand, within MMJJ_RS03300 at 625.244 kb on - strand, within MMJJ_RS03300 at 625.251 kb on - strand, within MMJJ_RS03300 at 625.321 kb on - strand, within MMJJ_RS03300 at 625.322 kb on + strand, within MMJJ_RS03300 at 625.343 kb on + strand, within MMJJ_RS03300 at 625.346 kb on + strand, within MMJJ_RS03300 at 625.375 kb on + strand, within MMJJ_RS03300 at 625.402 kb on + strand, within MMJJ_RS03300 at 625.413 kb on + strand, within MMJJ_RS03300 at 625.424 kb on - strand, within MMJJ_RS03300 at 625.424 kb on - strand, within MMJJ_RS03300 at 625.424 kb on - strand, within MMJJ_RS03300 at 625.427 kb on - strand, within MMJJ_RS03300 at 625.448 kb on - strand, within MMJJ_RS03300 at 625.448 kb on - strand, within MMJJ_RS03300 at 625.449 kb on + strand, within MMJJ_RS03300 at 625.487 kb on + strand, within MMJJ_RS03300 at 625.495 kb on - strand, within MMJJ_RS03300 at 625.512 kb on - strand, within MMJJ_RS03300 at 625.514 kb on - strand, within MMJJ_RS03300 at 625.514 kb on - strand, within MMJJ_RS03300 at 625.514 kb on - strand, within MMJJ_RS03300 at 625.515 kb on + strand, within MMJJ_RS03300 at 625.515 kb on + strand, within MMJJ_RS03300 at 625.547 kb on + strand, within MMJJ_RS03300 at 625.558 kb on + strand, within MMJJ_RS03300 at 625.582 kb on - strand, within MMJJ_RS03300 at 625.620 kb on - strand, within MMJJ_RS03300 at 625.644 kb on - strand, within MMJJ_RS03300 at 625.644 kb on - strand, within MMJJ_RS03300 at 625.686 kb on + strand, within MMJJ_RS03300 at 625.733 kb on - strand, within MMJJ_RS03300 at 625.776 kb on - strand, within MMJJ_RS03300 at 625.882 kb on + strand, within MMJJ_RS03300 at 625.960 kb on - strand, within MMJJ_RS03300 at 625.967 kb on - strand, within MMJJ_RS03300 at 625.967 kb on - strand, within MMJJ_RS03300 at 625.978 kb on - strand, within MMJJ_RS03300 at 625.978 kb on - strand, within MMJJ_RS03300 at 625.978 kb on - strand, within MMJJ_RS03300 at 625.978 kb on - strand, within MMJJ_RS03300 at 625.979 kb on + strand, within MMJJ_RS03300 at 625.984 kb on - strand, within MMJJ_RS03300 at 625.984 kb on - strand, within MMJJ_RS03300 at 625.984 kb on - strand, within MMJJ_RS03300 at 625.985 kb on + strand, within MMJJ_RS03300 at 625.985 kb on + strand, within MMJJ_RS03300 at 626.013 kb on + strand at 626.015 kb on + strand at 626.018 kb on + strand at 626.026 kb on - strand at 626.028 kb on - strand at 626.028 kb on - strand at 626.028 kb on - strand at 626.029 kb on + strand at 626.029 kb on + strand at 626.029 kb on + strand at 626.033 kb on + strand at 626.033 kb on + strand at 626.033 kb on + strand at 626.033 kb on + strand at 626.048 kb on + strand at 626.055 kb on + strand at 626.055 kb on + strand at 626.072 kb on + strand at 626.105 kb on - strand at 626.122 kb on + strand at 626.133 kb on - strand at 626.147 kb on + strand at 626.150 kb on - strand at 626.150 kb on - strand at 626.150 kb on - strand at 626.151 kb on + strand at 626.151 kb on + strand at 626.152 kb on - strand at 626.152 kb on - strand at 626.153 kb on + strand at 626.154 kb on - strand at 626.154 kb on - strand at 626.154 kb on - strand at 626.154 kb on - strand at 626.154 kb on - strand at 626.155 kb on + strand at 626.155 kb on + strand at 626.155 kb on + strand at 626.155 kb on + strand at 626.155 kb on + strand at 626.155 kb on + strand at 626.155 kb on + strand at 626.159 kb on + strand at 626.167 kb on - strand at 626.167 kb on - strand at 626.167 kb on - strand at 626.167 kb on - strand at 626.167 kb on - strand at 626.168 kb on + strand at 626.168 kb on + strand at 626.168 kb on + strand at 626.168 kb on + strand at 626.168 kb on + strand at 626.168 kb on + strand at 626.168 kb on + strand at 626.168 kb on + strand at 626.171 kb on - strand at 626.171 kb on - strand at 626.172 kb on + strand at 626.172 kb on + strand at 626.172 kb on + strand at 626.172 kb on + strand at 626.172 kb on + strand at 626.198 kb on - strand at 626.226 kb on + strand at 626.226 kb on + strand at 626.252 kb on - strand at 626.252 kb on - strand at 626.254 kb on - strand at 626.254 kb on - strand at 626.254 kb on - strand at 626.254 kb on - strand at 626.284 kb on - strand at 626.314 kb on - strand at 626.315 kb on + strand at 626.315 kb on + strand at 626.333 kb on - strand at 626.334 kb on + strand at 626.369 kb on - strand at 626.373 kb on - strand at 626.386 kb on - strand at 626.397 kb on + strand at 626.408 kb on - strand at 626.431 kb on - strand at 626.431 kb on - strand at 626.443 kb on - strand at 626.449 kb on - strand at 626.450 kb on + strand at 626.450 kb on + strand at 626.454 kb on + strand at 626.455 kb on - strand at 626.455 kb on - strand at 626.456 kb on + strand at 626.456 kb on + strand at 626.460 kb on - strand at 626.460 kb on - strand at 626.460 kb on - strand at 626.460 kb on - strand at 626.461 kb on + strand at 626.461 kb on + strand at 626.466 kb on + strand at 626.466 kb on + strand at 626.468 kb on - strand at 626.475 kb on + strand at 626.513 kb on - strand at 626.517 kb on - strand at 626.530 kb on - strand at 626.530 kb on - strand at 626.531 kb on + strand at 626.626 kb on - strand at 626.688 kb on + strand at 626.688 kb on + strand at 626.688 kb on + strand at 626.688 kb on + strand at 626.713 kb on - strand at 626.714 kb on + strand at 626.714 kb on + strand at 626.720 kb on - strand at 626.721 kb on + strand at 626.750 kb on - strand at 626.751 kb on + strand at 626.754 kb on + strand at 626.754 kb on + strand at 626.756 kb on - strand at 626.756 kb on - strand at 626.758 kb on - strand at 626.758 kb on - strand at 626.758 kb on - strand at 626.759 kb on + strand at 626.759 kb on + strand at 626.759 kb on + strand at 626.811 kb on - strand at 626.812 kb on + strand at 626.836 kb on - strand at 626.924 kb on + strand at 626.929 kb on + strand at 627.025 kb on + strand at 627.054 kb on + strand at 627.083 kb on - strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.085 kb on - strand at 627.086 kb on + strand at 627.086 kb on + strand at 627.086 kb on + strand at 627.088 kb on + strand at 627.102 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 623,940 - MMJJ_RS03290 0.76 -1.5 623,940 - MMJJ_RS03290 0.76 -2.4 623,944 - MMJJ_RS03290 0.77 -2.6 623,945 + MMJJ_RS03290 0.77 +0.6 624,010 - MMJJ_RS03290 0.82 +1.0 624,038 + MMJJ_RS03290 0.84 +1.2 624,040 - MMJJ_RS03290 0.84 -1.9 624,070 + MMJJ_RS03290 0.86 +0.7 624,082 - MMJJ_RS03290 0.87 -0.2 624,085 - MMJJ_RS03290 0.88 +1.1 624,087 - MMJJ_RS03290 0.88 +2.4 624,102 - MMJJ_RS03290 0.89 +2.6 624,102 - MMJJ_RS03290 0.89 +0.3 624,102 - MMJJ_RS03290 0.89 -0.7 624,103 + MMJJ_RS03290 0.89 +0.1 624,103 + MMJJ_RS03290 0.89 +0.3 624,103 + MMJJ_RS03290 0.89 -0.7 624,135 - -1.4 624,138 - +2.5 624,151 - +1.1 624,151 - +1.8 624,151 - -0.3 624,152 + -1.4 624,195 - -1.2 624,195 - -2.6 624,195 - -1.7 624,196 + +0.8 624,196 + +3.3 624,199 - -0.6 624,199 - -1.5 624,199 - -2.1 624,200 + +1.5 624,202 - +1.3 624,214 - -1.1 624,214 - -1.2 624,214 - -0.1 624,215 + -1.8 624,295 - +3.4 624,295 - +0.0 624,295 - +0.3 624,295 - -2.1 624,296 + -1.0 624,419 + MMJJ_RS03295 0.23 +1.3 624,496 - MMJJ_RS03295 0.35 +1.3 624,540 - MMJJ_RS03295 0.41 -0.2 624,541 + MMJJ_RS03295 0.42 -0.3 624,584 - MMJJ_RS03295 0.48 +0.8 624,584 - MMJJ_RS03295 0.48 -2.0 624,616 - MMJJ_RS03295 0.53 -1.9 624,617 + MMJJ_RS03295 0.53 +0.3 624,631 - MMJJ_RS03295 0.56 +0.8 624,631 - MMJJ_RS03295 0.56 -3.0 624,631 - MMJJ_RS03295 0.56 -0.2 624,632 + MMJJ_RS03295 0.56 -1.9 624,632 + MMJJ_RS03295 0.56 -1.0 624,632 + MMJJ_RS03295 0.56 -0.2 624,640 - MMJJ_RS03295 0.57 -0.6 624,641 + MMJJ_RS03295 0.57 -1.7 624,641 + MMJJ_RS03295 0.57 +0.8 624,641 + MMJJ_RS03295 0.57 -3.1 624,641 + MMJJ_RS03295 0.57 +0.1 624,641 + MMJJ_RS03295 0.57 -1.7 624,642 - MMJJ_RS03295 0.57 -3.1 624,642 - MMJJ_RS03295 0.57 -0.4 624,643 + MMJJ_RS03295 0.57 -0.5 624,643 + MMJJ_RS03295 0.57 -0.5 624,644 - MMJJ_RS03295 0.58 +0.1 624,684 - MMJJ_RS03295 0.64 -2.7 624,685 + MMJJ_RS03295 0.64 -2.2 624,701 - MMJJ_RS03295 0.66 -0.0 624,702 + MMJJ_RS03295 0.67 +0.3 624,752 + MMJJ_RS03295 0.74 -0.9 624,762 - MMJJ_RS03295 0.76 -0.8 624,787 - MMJJ_RS03295 0.80 -1.0 624,788 + MMJJ_RS03295 0.80 -1.7 624,792 + MMJJ_RS03295 0.80 -1.8 624,844 + MMJJ_RS03295 0.89 -0.6 624,844 + MMJJ_RS03295 0.89 -3.1 624,877 - +0.9 624,877 - -0.4 624,877 - +0.2 624,877 - +0.3 624,877 - -2.0 624,878 + -1.3 624,878 + -1.2 624,878 + -2.3 624,878 + -1.5 624,878 + -1.7 624,884 - -1.0 624,885 + -1.6 624,910 - +2.3 624,936 + +3.6 624,936 + +1.3 624,949 - +3.5 624,975 - -1.8 624,976 + +3.4 625,046 + MMJJ_RS03300 0.10 +1.4 625,144 - MMJJ_RS03300 0.19 -1.2 625,147 - MMJJ_RS03300 0.19 -2.0 625,203 + MMJJ_RS03300 0.24 +0.3 625,244 - MMJJ_RS03300 0.27 -1.8 625,251 - MMJJ_RS03300 0.28 -2.2 625,321 - MMJJ_RS03300 0.34 +2.6 625,322 + MMJJ_RS03300 0.34 +3.7 625,343 + MMJJ_RS03300 0.35 +0.2 625,346 + MMJJ_RS03300 0.36 +0.6 625,375 + MMJJ_RS03300 0.38 -0.6 625,402 + MMJJ_RS03300 0.40 +0.5 625,413 + MMJJ_RS03300 0.41 -1.1 625,424 - MMJJ_RS03300 0.42 -4.1 625,424 - MMJJ_RS03300 0.42 -2.0 625,424 - MMJJ_RS03300 0.42 +1.1 625,427 - MMJJ_RS03300 0.43 +1.8 625,448 - MMJJ_RS03300 0.44 -0.4 625,448 - MMJJ_RS03300 0.44 +3.4 625,449 + MMJJ_RS03300 0.44 +1.1 625,487 + MMJJ_RS03300 0.48 -1.9 625,495 - MMJJ_RS03300 0.48 +0.6 625,512 - MMJJ_RS03300 0.50 +0.8 625,514 - MMJJ_RS03300 0.50 +0.7 625,514 - MMJJ_RS03300 0.50 +1.3 625,514 - MMJJ_RS03300 0.50 -1.1 625,515 + MMJJ_RS03300 0.50 +2.8 625,515 + MMJJ_RS03300 0.50 +2.6 625,547 + MMJJ_RS03300 0.53 +1.5 625,558 + MMJJ_RS03300 0.54 -3.1 625,582 - MMJJ_RS03300 0.56 -2.5 625,620 - MMJJ_RS03300 0.59 +1.2 625,644 - MMJJ_RS03300 0.61 -3.2 625,644 - MMJJ_RS03300 0.61 -2.2 625,686 + MMJJ_RS03300 0.64 -2.2 625,733 - MMJJ_RS03300 0.68 -1.1 625,776 - MMJJ_RS03300 0.72 -0.6 625,882 + MMJJ_RS03300 0.81 -2.0 625,960 - MMJJ_RS03300 0.88 -1.0 625,967 - MMJJ_RS03300 0.88 +0.3 625,967 - MMJJ_RS03300 0.88 -0.2 625,978 - MMJJ_RS03300 0.89 +0.6 625,978 - MMJJ_RS03300 0.89 +3.3 625,978 - MMJJ_RS03300 0.89 -1.0 625,978 - MMJJ_RS03300 0.89 -3.2 625,979 + MMJJ_RS03300 0.89 -1.7 625,984 - MMJJ_RS03300 0.90 +2.3 625,984 - MMJJ_RS03300 0.90 -1.2 625,984 - MMJJ_RS03300 0.90 -0.7 625,985 + MMJJ_RS03300 0.90 -1.1 625,985 + MMJJ_RS03300 0.90 -1.5 626,013 + -1.7 626,015 + +2.3 626,018 + -3.1 626,026 - -2.7 626,028 - +1.1 626,028 - +2.9 626,028 - -2.9 626,029 + +2.5 626,029 + -2.5 626,029 + -2.5 626,033 + -1.8 626,033 + -0.7 626,033 + -1.1 626,033 + +0.2 626,048 + +1.0 626,055 + -2.2 626,055 + -3.2 626,072 + -1.7 626,105 - -0.1 626,122 + +0.8 626,133 - +1.8 626,147 + +2.9 626,150 - +3.0 626,150 - -1.1 626,150 - +2.7 626,151 + +0.6 626,151 + +0.6 626,152 - -0.2 626,152 - -1.9 626,153 + -1.6 626,154 - -1.5 626,154 - +0.5 626,154 - -1.8 626,154 - +1.3 626,154 - -1.5 626,155 + -1.0 626,155 + -0.4 626,155 + +0.3 626,155 + +2.4 626,155 + +3.2 626,155 + -1.4 626,155 + -1.5 626,159 + +0.8 626,167 - -1.0 626,167 - +0.2 626,167 - +1.8 626,167 - -1.6 626,167 - -1.5 626,168 + -0.9 626,168 + -1.7 626,168 + -2.7 626,168 + -3.4 626,168 + +0.2 626,168 + +3.0 626,168 + +2.3 626,168 + -0.7 626,171 - -0.5 626,171 - -2.2 626,172 + +0.3 626,172 + -2.7 626,172 + -1.9 626,172 + -1.2 626,172 + -1.6 626,198 - -2.3 626,226 + +1.3 626,226 + +1.0 626,252 - -2.2 626,252 - -0.8 626,254 - -2.2 626,254 - +2.9 626,254 - -1.5 626,254 - +0.8 626,284 - +0.3 626,314 - -0.7 626,315 + +2.3 626,315 + -4.0 626,333 - -2.7 626,334 + -2.4 626,369 - -0.2 626,373 - +1.3 626,386 - -4.1 626,397 + +2.0 626,408 - -2.2 626,431 - -0.4 626,431 - +0.8 626,443 - -0.7 626,449 - +4.5 626,450 + +2.5 626,450 + +0.4 626,454 + -1.1 626,455 - +2.3 626,455 - +1.4 626,456 + -3.0 626,456 + +1.5 626,460 - +2.2 626,460 - -3.2 626,460 - -1.8 626,460 - -0.4 626,461 + +4.4 626,461 + -1.9 626,466 + -1.1 626,466 + -0.7 626,468 - +2.8 626,475 + -1.2 626,513 - +0.0 626,517 - +0.9 626,530 - +2.9 626,530 - +0.8 626,531 + -1.7 626,626 - -2.6 626,688 + -0.2 626,688 + -0.2 626,688 + +2.1 626,688 + +0.9 626,713 - -1.5 626,714 + +2.5 626,714 + +3.2 626,720 - +2.1 626,721 + +1.6 626,750 - -2.0 626,751 + -0.8 626,754 + +0.7 626,754 + +0.0 626,756 - -2.7 626,756 - +1.3 626,758 - +0.1 626,758 - -0.7 626,758 - -0.2 626,759 + +2.9 626,759 + +1.1 626,759 + -1.0 626,811 - +1.7 626,812 + +0.8 626,836 - -0.3 626,924 + +0.7 626,929 + -0.2 627,025 + +0.1 627,054 + +2.2 627,083 - -2.3 627,084 + +1.2 627,084 + -0.2 627,084 + +0.5 627,084 + +0.1 627,084 + +2.2 627,084 + -0.2 627,085 - +0.8 627,086 + -2.7 627,086 + +2.5 627,086 + -2.0 627,088 + -0.5 627,102 + +1.5
Or see this region's nucleotide sequence