Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03245 and MMJJ_RS03250 are separated by 154 nucleotides MMJJ_RS03250 and MMJJ_RS03255 are separated by 172 nucleotides MMJJ_RS03255 and MMJJ_RS03260 are separated by 99 nucleotides MMJJ_RS03260 and MMJJ_RS03265 are separated by 276 nucleotides
MMJJ_RS03245: MMJJ_RS03245 - UMP kinase, at 613,994 to 614,671
_RS03245
MMJJ_RS03250: MMJJ_RS03250 - DUF2116 family Zn-ribbon domain-containing protein, at 614,826 to 615,008
_RS03250
MMJJ_RS03255: MMJJ_RS03255 - tRNA-Met, at 615,181 to 615,259
_RS03255
MMJJ_RS03260: MMJJ_RS03260 - tRNA-Met, at 615,359 to 615,437
_RS03260
MMJJ_RS03265: MMJJ_RS03265 - S-layer protein, at 615,714 to 617,330
_RS03265
Position (kb)
614
615
616 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 613.845 kb on - strand at 613.846 kb on + strand at 613.858 kb on - strand at 613.859 kb on + strand at 613.864 kb on + strand at 613.864 kb on + strand at 613.917 kb on + strand at 613.930 kb on + strand at 614.002 kb on + strand at 614.150 kb on - strand, within MMJJ_RS03245 at 614.432 kb on - strand, within MMJJ_RS03245 at 614.663 kb on + strand at 614.686 kb on + strand at 614.773 kb on - strand at 614.777 kb on - strand at 614.777 kb on - strand at 614.804 kb on - strand at 614.805 kb on + strand at 614.805 kb on + strand at 614.807 kb on - strand at 614.807 kb on - strand at 614.848 kb on - strand, within MMJJ_RS03250 at 614.848 kb on - strand, within MMJJ_RS03250 at 614.848 kb on - strand, within MMJJ_RS03250 at 614.992 kb on + strand at 615.042 kb on + strand at 615.058 kb on - strand at 615.265 kb on - strand at 615.266 kb on + strand at 615.343 kb on - strand at 615.346 kb on - strand at 615.346 kb on - strand at 615.346 kb on - strand at 615.347 kb on + strand at 615.459 kb on - strand at 615.459 kb on - strand at 615.498 kb on + strand at 615.498 kb on + strand at 615.567 kb on - strand at 615.569 kb on - strand at 615.637 kb on + strand at 615.694 kb on - strand at 615.716 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 613,845 - -1.8 613,846 + +2.7 613,858 - -2.3 613,859 + -0.7 613,864 + -4.9 613,864 + -0.8 613,917 + -0.3 613,930 + +0.3 614,002 + +0.3 614,150 - MMJJ_RS03245 0.23 +0.3 614,432 - MMJJ_RS03245 0.65 -1.8 614,663 + -2.8 614,686 + +2.3 614,773 - +0.8 614,777 - +3.0 614,777 - -3.7 614,804 - -2.1 614,805 + +0.1 614,805 + -2.9 614,807 - -1.8 614,807 - +0.5 614,848 - MMJJ_RS03250 0.12 +0.6 614,848 - MMJJ_RS03250 0.12 +0.9 614,848 - MMJJ_RS03250 0.12 -2.8 614,992 + +1.5 615,042 + +1.0 615,058 - -1.3 615,265 - +0.2 615,266 + -0.7 615,343 - -1.0 615,346 - +0.7 615,346 - -2.0 615,346 - +4.9 615,347 + -0.3 615,459 - +2.6 615,459 - -0.9 615,498 + +0.0 615,498 + +0.2 615,567 - +3.3 615,569 - -1.8 615,637 + +0.2 615,694 - -2.7 615,716 + -0.9
Or see this region's nucleotide sequence