Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS03225

Experiment: McCas_formate_complete Antimycin A 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS03215 and MMJJ_RS03220 are separated by 65 nucleotidesMMJJ_RS03220 and MMJJ_RS03225 are separated by 86 nucleotidesMMJJ_RS03225 and MMJJ_RS03230 are separated by 2 nucleotides MMJJ_RS03215: MMJJ_RS03215 - alanine--glyoxylate aminotransferase family protein, at 609,411 to 610,559 _RS03215 MMJJ_RS03220: MMJJ_RS03220 - inosine/xanthosine triphosphatase, at 610,625 to 611,284 _RS03220 MMJJ_RS03225: MMJJ_RS03225 - 4Fe-4S binding protein, at 611,371 to 611,535 _RS03225 MMJJ_RS03230: MMJJ_RS03230 - NAD(P)/FAD-dependent oxidoreductase, at 611,538 to 612,710 _RS03230 Position (kb) 611 612Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 610.550 kb on + strandat 610.557 kb on - strandat 610.565 kb on + strandat 610.581 kb on - strandat 610.600 kb on + strandat 610.604 kb on - strandat 610.605 kb on + strandat 610.605 kb on + strandat 610.623 kb on - strandat 610.624 kb on + strandat 610.690 kb on + strandat 610.690 kb on + strandat 610.766 kb on + strand, within MMJJ_RS03220at 610.845 kb on - strand, within MMJJ_RS03220at 610.845 kb on - strand, within MMJJ_RS03220at 610.846 kb on + strand, within MMJJ_RS03220at 610.846 kb on + strand, within MMJJ_RS03220at 610.907 kb on + strand, within MMJJ_RS03220at 610.916 kb on - strand, within MMJJ_RS03220at 610.916 kb on - strand, within MMJJ_RS03220at 610.916 kb on - strand, within MMJJ_RS03220at 610.916 kb on - strand, within MMJJ_RS03220at 610.917 kb on + strand, within MMJJ_RS03220at 610.917 kb on + strand, within MMJJ_RS03220at 610.917 kb on + strand, within MMJJ_RS03220at 610.918 kb on - strand, within MMJJ_RS03220at 610.948 kb on + strand, within MMJJ_RS03220at 611.028 kb on - strand, within MMJJ_RS03220at 611.028 kb on - strand, within MMJJ_RS03220at 611.029 kb on + strand, within MMJJ_RS03220at 611.029 kb on + strand, within MMJJ_RS03220at 611.029 kb on + strand, within MMJJ_RS03220at 611.029 kb on + strand, within MMJJ_RS03220at 611.030 kb on - strand, within MMJJ_RS03220at 611.030 kb on - strand, within MMJJ_RS03220at 611.031 kb on + strand, within MMJJ_RS03220at 611.032 kb on - strand, within MMJJ_RS03220at 611.032 kb on - strand, within MMJJ_RS03220at 611.032 kb on - strand, within MMJJ_RS03220at 611.033 kb on + strand, within MMJJ_RS03220at 611.033 kb on + strand, within MMJJ_RS03220at 611.086 kb on - strand, within MMJJ_RS03220at 611.188 kb on - strand, within MMJJ_RS03220at 611.200 kb on - strand, within MMJJ_RS03220at 611.200 kb on - strand, within MMJJ_RS03220at 611.200 kb on - strand, within MMJJ_RS03220at 611.200 kb on - strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.203 kb on + strand, within MMJJ_RS03220at 611.203 kb on + strand, within MMJJ_RS03220at 611.203 kb on + strand, within MMJJ_RS03220at 611.207 kb on + strand, within MMJJ_RS03220at 611.217 kb on - strand, within MMJJ_RS03220at 611.217 kb on - strand, within MMJJ_RS03220at 611.217 kb on - strand, within MMJJ_RS03220at 611.217 kb on - strand, within MMJJ_RS03220at 611.218 kb on + strand, within MMJJ_RS03220at 611.218 kb on + strand, within MMJJ_RS03220at 611.218 kb on + strand, within MMJJ_RS03220at 611.218 kb on + strand, within MMJJ_RS03220at 611.221 kb on - strandat 611.235 kb on - strandat 611.235 kb on - strandat 611.236 kb on + strandat 611.255 kb on - strandat 611.256 kb on + strandat 611.275 kb on + strandat 611.282 kb on - strandat 611.292 kb on - strandat 611.295 kb on - strandat 611.295 kb on - strandat 611.296 kb on + strandat 611.296 kb on + strandat 611.317 kb on - strandat 611.318 kb on + strandat 611.318 kb on + strandat 611.321 kb on - strandat 611.321 kb on - strandat 611.321 kb on - strandat 611.324 kb on + strandat 611.324 kb on + strandat 611.324 kb on + strandat 611.342 kb on + strandat 611.420 kb on - strand, within MMJJ_RS03225at 611.425 kb on + strand, within MMJJ_RS03225at 611.499 kb on - strand, within MMJJ_RS03225at 611.507 kb on - strand, within MMJJ_RS03225at 611.507 kb on - strand, within MMJJ_RS03225at 611.507 kb on - strand, within MMJJ_RS03225at 611.560 kb on + strandat 611.641 kb on - strandat 612.012 kb on + strand, within MMJJ_RS03230at 612.153 kb on - strand, within MMJJ_RS03230at 612.180 kb on + strand, within MMJJ_RS03230at 612.191 kb on + strand, within MMJJ_RS03230at 612.235 kb on - strand, within MMJJ_RS03230at 612.235 kb on - strand, within MMJJ_RS03230at 612.236 kb on + strand, within MMJJ_RS03230at 612.385 kb on - strand, within MMJJ_RS03230at 612.410 kb on + strand, within MMJJ_RS03230at 612.477 kb on - strand, within MMJJ_RS03230

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM
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610,550 + -2.8
610,557 - -0.3
610,565 + +0.2
610,581 - +0.9
610,600 + -1.8
610,604 - +0.7
610,605 + +2.9
610,605 + -2.3
610,623 - +0.2
610,624 + -0.8
610,690 + +0.9
610,690 + -2.1
610,766 + MMJJ_RS03220 0.21 +2.2
610,845 - MMJJ_RS03220 0.33 +0.2
610,845 - MMJJ_RS03220 0.33 +1.4
610,846 + MMJJ_RS03220 0.33 -0.3
610,846 + MMJJ_RS03220 0.33 -1.0
610,907 + MMJJ_RS03220 0.43 -1.3
610,916 - MMJJ_RS03220 0.44 -2.2
610,916 - MMJJ_RS03220 0.44 -3.0
610,916 - MMJJ_RS03220 0.44 -1.2
610,916 - MMJJ_RS03220 0.44 +0.1
610,917 + MMJJ_RS03220 0.44 +0.4
610,917 + MMJJ_RS03220 0.44 -0.3
610,917 + MMJJ_RS03220 0.44 -1.5
610,918 - MMJJ_RS03220 0.44 +1.6
610,948 + MMJJ_RS03220 0.49 -1.6
611,028 - MMJJ_RS03220 0.61 +1.0
611,028 - MMJJ_RS03220 0.61 +0.4
611,029 + MMJJ_RS03220 0.61 +0.4
611,029 + MMJJ_RS03220 0.61 -0.7
611,029 + MMJJ_RS03220 0.61 -2.6
611,029 + MMJJ_RS03220 0.61 -0.4
611,030 - MMJJ_RS03220 0.61 -1.8
611,030 - MMJJ_RS03220 0.61 -2.1
611,031 + MMJJ_RS03220 0.62 -0.5
611,032 - MMJJ_RS03220 0.62 -0.6
611,032 - MMJJ_RS03220 0.62 +3.8
611,032 - MMJJ_RS03220 0.62 -1.3
611,033 + MMJJ_RS03220 0.62 -0.6
611,033 + MMJJ_RS03220 0.62 -3.4
611,086 - MMJJ_RS03220 0.70 -3.3
611,188 - MMJJ_RS03220 0.85 +1.4
611,200 - MMJJ_RS03220 0.87 -1.4
611,200 - MMJJ_RS03220 0.87 -0.3
611,200 - MMJJ_RS03220 0.87 -2.0
611,200 - MMJJ_RS03220 0.87 -1.7
611,201 + MMJJ_RS03220 0.87 -2.6
611,201 + MMJJ_RS03220 0.87 +0.1
611,201 + MMJJ_RS03220 0.87 -1.1
611,201 + MMJJ_RS03220 0.87 +2.3
611,201 + MMJJ_RS03220 0.87 -1.1
611,202 - MMJJ_RS03220 0.87 +2.5
611,202 - MMJJ_RS03220 0.87 -0.4
611,202 - MMJJ_RS03220 0.87 +2.4
611,202 - MMJJ_RS03220 0.87 -1.3
611,202 - MMJJ_RS03220 0.87 -1.1
611,203 + MMJJ_RS03220 0.88 -0.3
611,203 + MMJJ_RS03220 0.88 -2.6
611,203 + MMJJ_RS03220 0.88 -1.9
611,207 + MMJJ_RS03220 0.88 -1.7
611,217 - MMJJ_RS03220 0.90 -0.8
611,217 - MMJJ_RS03220 0.90 -0.9
611,217 - MMJJ_RS03220 0.90 -1.3
611,217 - MMJJ_RS03220 0.90 -1.0
611,218 + MMJJ_RS03220 0.90 +0.7
611,218 + MMJJ_RS03220 0.90 -1.8
611,218 + MMJJ_RS03220 0.90 -2.6
611,218 + MMJJ_RS03220 0.90 -0.7
611,221 - -2.1
611,235 - +0.5
611,235 - -1.7
611,236 + +0.6
611,255 - -0.5
611,256 + -1.6
611,275 + +1.9
611,282 - -2.4
611,292 - -1.4
611,295 - -1.9
611,295 - -1.0
611,296 + +0.2
611,296 + -2.3
611,317 - -0.4
611,318 + -0.7
611,318 + +0.3
611,321 - -1.7
611,321 - -1.0
611,321 - -0.3
611,324 + -1.5
611,324 + -1.3
611,324 + -0.3
611,342 + +0.5
611,420 - MMJJ_RS03225 0.30 +0.3
611,425 + MMJJ_RS03225 0.33 -1.0
611,499 - MMJJ_RS03225 0.78 -0.7
611,507 - MMJJ_RS03225 0.82 -0.3
611,507 - MMJJ_RS03225 0.82 -1.8
611,507 - MMJJ_RS03225 0.82 +0.7
611,560 + -0.3
611,641 - +0.3
612,012 + MMJJ_RS03230 0.40 -0.3
612,153 - MMJJ_RS03230 0.52 +0.3
612,180 + MMJJ_RS03230 0.55 -1.3
612,191 + MMJJ_RS03230 0.56 +1.3
612,235 - MMJJ_RS03230 0.59 -1.3
612,235 - MMJJ_RS03230 0.59 -0.7
612,236 + MMJJ_RS03230 0.60 +0.7
612,385 - MMJJ_RS03230 0.72 -2.1
612,410 + MMJJ_RS03230 0.74 -2.3
612,477 - MMJJ_RS03230 0.80 -1.4

Or see this region's nucleotide sequence