Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03185 and MMJJ_RS03190 are separated by 199 nucleotides MMJJ_RS03190 and MMJJ_RS03195 are separated by 241 nucleotides MMJJ_RS03195 and MMJJ_RS03200 are separated by 15 nucleotides
MMJJ_RS03185: MMJJ_RS03185 - alanine--tRNA ligase, at 601,763 to 604,441
_RS03185
MMJJ_RS03190: MMJJ_RS03190 - phosphopyruvate hydratase, at 604,641 to 605,933
_RS03190
MMJJ_RS03195: MMJJ_RS03195 - hypothetical protein, at 606,175 to 606,453
_RS03195
MMJJ_RS03200: MMJJ_RS03200 - uroporphyrinogen-III synthase, at 606,469 to 607,203
_RS03200
Position (kb) 604
605
606 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 604.330 kb on - strand at 604.331 kb on + strand at 604.375 kb on - strand at 604.497 kb on + strand at 604.541 kb on - strand at 604.542 kb on + strand at 604.542 kb on + strand at 604.546 kb on - strand at 604.547 kb on + strand at 604.548 kb on - strand at 604.562 kb on + strand at 604.586 kb on - strand at 604.595 kb on - strand at 604.600 kb on + strand at 604.626 kb on - strand at 604.632 kb on - strand at 604.632 kb on - strand at 604.633 kb on + strand at 604.643 kb on + strand at 604.643 kb on + strand at 605.171 kb on + strand, within MMJJ_RS03190 at 605.304 kb on - strand at 605.310 kb on - strand at 605.311 kb on + strand, within MMJJ_RS03190 at 605.340 kb on - strand, within MMJJ_RS03190 at 605.360 kb on + strand, within MMJJ_RS03190 at 605.498 kb on + strand, within MMJJ_RS03190 at 605.518 kb on - strand, within MMJJ_RS03190 at 605.581 kb on + strand, within MMJJ_RS03190 at 605.679 kb on - strand, within MMJJ_RS03190 at 605.971 kb on - strand at 605.998 kb on + strand at 606.027 kb on - strand at 606.043 kb on + strand at 606.099 kb on + strand at 606.106 kb on + strand at 606.118 kb on - strand at 606.122 kb on - strand at 606.122 kb on - strand at 606.123 kb on + strand at 606.123 kb on + strand at 606.124 kb on - strand at 606.142 kb on - strand at 606.155 kb on + strand at 606.155 kb on + strand at 606.172 kb on + strand at 606.195 kb on + strand at 606.195 kb on + strand at 606.198 kb on - strand at 606.216 kb on - strand, within MMJJ_RS03195 at 606.276 kb on + strand, within MMJJ_RS03195 at 606.346 kb on - strand, within MMJJ_RS03195 at 606.347 kb on + strand, within MMJJ_RS03195 at 606.414 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.618 kb on + strand, within MMJJ_RS03200 at 606.775 kb on - strand, within MMJJ_RS03200 at 606.775 kb on - strand at 606.859 kb on + strand, within MMJJ_RS03200
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 604,330 - +1.3 604,331 + -0.3 604,375 - -1.7 604,497 + -0.7 604,541 - -1.6 604,542 + +1.5 604,542 + +0.1 604,546 - +1.4 604,547 + +1.8 604,548 - +2.2 604,562 + +0.7 604,586 - +1.3 604,595 - -1.7 604,600 + -1.3 604,626 - +1.3 604,632 - +0.4 604,632 - +0.8 604,633 + -0.3 604,643 + +0.8 604,643 + +0.2 605,171 + MMJJ_RS03190 0.41 -0.3 605,304 - +1.3 605,310 - +1.3 605,311 + MMJJ_RS03190 0.52 -1.3 605,340 - MMJJ_RS03190 0.54 +1.3 605,360 + MMJJ_RS03190 0.56 -0.7 605,498 + MMJJ_RS03190 0.66 -0.7 605,518 - MMJJ_RS03190 0.68 -0.7 605,581 + MMJJ_RS03190 0.73 -0.3 605,679 - MMJJ_RS03190 0.80 -1.0 605,971 - +1.3 605,998 + +0.3 606,027 - +2.3 606,043 + -2.1 606,099 + -1.6 606,106 + +0.7 606,118 - +0.7 606,122 - -0.0 606,122 - -1.8 606,123 + +2.8 606,123 + -1.3 606,124 - -0.3 606,142 - -0.9 606,155 + +1.8 606,155 + -1.7 606,172 + +2.8 606,195 + +0.0 606,195 + +0.3 606,198 - -1.0 606,216 - MMJJ_RS03195 0.15 -1.5 606,276 + MMJJ_RS03195 0.36 -3.4 606,346 - MMJJ_RS03195 0.61 -1.8 606,347 + MMJJ_RS03195 0.62 -2.4 606,414 + MMJJ_RS03195 0.86 +2.2 606,422 + MMJJ_RS03195 0.89 -0.8 606,422 + MMJJ_RS03195 0.89 +2.5 606,422 + MMJJ_RS03195 0.89 -1.9 606,618 + MMJJ_RS03200 0.20 -1.0 606,775 - MMJJ_RS03200 0.42 +0.3 606,775 - +0.3 606,859 + MMJJ_RS03200 0.53 -0.7
Or see this region's nucleotide sequence