Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03085 and MMJJ_RS03090 are separated by 207 nucleotides MMJJ_RS03090 and MMJJ_RS03095 are separated by 87 nucleotides MMJJ_RS03095 and MMJJ_RS03100 are separated by 172 nucleotides
MMJJ_RS03085: MMJJ_RS03085 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 579,633 to 580,349
_RS03085
MMJJ_RS03090: MMJJ_RS03090 - ECF transporter S component, at 580,557 to 581,153
_RS03090
MMJJ_RS03095: MMJJ_RS03095 - bifunctional N(6)-L-threonylcarbamoyladenine synthase/serine/threonine protein kinase, at 581,241 to 582,884
_RS03095
MMJJ_RS03100: MMJJ_RS03100 - threonine--tRNA ligase, at 583,057 to 584,925
_RS03100
Position (kb) 581
582
583 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 580.402 kb on - strand at 580.434 kb on - strand at 580.435 kb on + strand at 580.436 kb on - strand at 580.436 kb on - strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.437 kb on + strand at 580.442 kb on - strand at 580.442 kb on - strand at 580.442 kb on - strand at 580.446 kb on - strand at 580.482 kb on + strand at 580.489 kb on - strand at 580.510 kb on + strand at 580.541 kb on - strand at 580.559 kb on + strand at 580.588 kb on + strand at 580.588 kb on + strand at 580.588 kb on + strand at 580.635 kb on - strand, within MMJJ_RS03090 at 580.636 kb on + strand, within MMJJ_RS03090 at 580.672 kb on + strand, within MMJJ_RS03090 at 580.672 kb on + strand, within MMJJ_RS03090 at 580.692 kb on - strand at 580.693 kb on + strand, within MMJJ_RS03090 at 580.693 kb on + strand, within MMJJ_RS03090 at 580.693 kb on + strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.729 kb on - strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.730 kb on + strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.731 kb on - strand, within MMJJ_RS03090 at 580.732 kb on + strand, within MMJJ_RS03090 at 580.732 kb on + strand, within MMJJ_RS03090 at 580.737 kb on - strand, within MMJJ_RS03090 at 580.737 kb on - strand, within MMJJ_RS03090 at 580.738 kb on + strand, within MMJJ_RS03090 at 580.738 kb on + strand, within MMJJ_RS03090 at 580.738 kb on + strand, within MMJJ_RS03090 at 580.738 kb on + strand, within MMJJ_RS03090 at 580.761 kb on + strand, within MMJJ_RS03090 at 580.777 kb on - strand, within MMJJ_RS03090 at 580.777 kb on - strand, within MMJJ_RS03090 at 580.778 kb on + strand, within MMJJ_RS03090 at 580.781 kb on + strand, within MMJJ_RS03090 at 580.781 kb on + strand, within MMJJ_RS03090 at 580.789 kb on + strand, within MMJJ_RS03090 at 580.789 kb on + strand, within MMJJ_RS03090 at 580.789 kb on + strand, within MMJJ_RS03090 at 580.789 kb on + strand, within MMJJ_RS03090 at 580.790 kb on - strand, within MMJJ_RS03090 at 580.790 kb on - strand, within MMJJ_RS03090 at 580.791 kb on + strand, within MMJJ_RS03090 at 580.791 kb on + strand, within MMJJ_RS03090 at 580.834 kb on + strand, within MMJJ_RS03090 at 580.834 kb on + strand, within MMJJ_RS03090 at 580.835 kb on - strand, within MMJJ_RS03090 at 580.843 kb on - strand, within MMJJ_RS03090 at 580.844 kb on + strand, within MMJJ_RS03090 at 580.874 kb on - strand, within MMJJ_RS03090 at 580.908 kb on - strand, within MMJJ_RS03090 at 580.909 kb on + strand, within MMJJ_RS03090 at 580.918 kb on - strand, within MMJJ_RS03090 at 580.918 kb on - strand, within MMJJ_RS03090 at 580.919 kb on + strand, within MMJJ_RS03090 at 580.922 kb on + strand, within MMJJ_RS03090 at 580.922 kb on + strand, within MMJJ_RS03090 at 580.933 kb on - strand, within MMJJ_RS03090 at 580.933 kb on - strand, within MMJJ_RS03090 at 581.015 kb on - strand, within MMJJ_RS03090 at 581.015 kb on - strand, within MMJJ_RS03090 at 581.016 kb on + strand, within MMJJ_RS03090 at 581.016 kb on + strand, within MMJJ_RS03090 at 581.028 kb on + strand, within MMJJ_RS03090 at 581.028 kb on + strand, within MMJJ_RS03090 at 581.031 kb on - strand, within MMJJ_RS03090 at 581.053 kb on - strand, within MMJJ_RS03090 at 581.053 kb on - strand, within MMJJ_RS03090 at 581.054 kb on + strand, within MMJJ_RS03090 at 581.054 kb on + strand, within MMJJ_RS03090 at 581.054 kb on + strand, within MMJJ_RS03090 at 581.058 kb on + strand, within MMJJ_RS03090 at 581.074 kb on + strand, within MMJJ_RS03090 at 581.074 kb on + strand, within MMJJ_RS03090 at 581.107 kb on - strand at 581.142 kb on - strand at 581.153 kb on - strand at 581.153 kb on - strand at 581.154 kb on + strand at 581.156 kb on - strand at 581.156 kb on - strand at 581.157 kb on + strand at 581.162 kb on + strand at 581.162 kb on + strand at 581.172 kb on - strand at 581.173 kb on + strand at 581.184 kb on + strand at 581.197 kb on - strand at 581.197 kb on - strand at 581.197 kb on - strand at 581.198 kb on + strand at 581.198 kb on + strand at 581.198 kb on + strand at 581.201 kb on - strand at 581.201 kb on - strand at 581.201 kb on - strand at 581.202 kb on + strand at 581.202 kb on + strand at 581.202 kb on + strand at 581.202 kb on + strand at 581.203 kb on - strand at 581.206 kb on + strand at 581.230 kb on + strand at 581.248 kb on - strand at 581.249 kb on + strand at 581.249 kb on + strand at 581.249 kb on + strand at 581.249 kb on + strand at 581.249 kb on + strand at 581.306 kb on + strand at 581.330 kb on - strand at 581.415 kb on - strand, within MMJJ_RS03095 at 582.140 kb on - strand, within MMJJ_RS03095 at 582.143 kb on - strand, within MMJJ_RS03095 at 582.335 kb on + strand, within MMJJ_RS03095 at 582.484 kb on + strand, within MMJJ_RS03095 at 582.487 kb on - strand, within MMJJ_RS03095 at 582.946 kb on + strand at 582.964 kb on - strand at 583.003 kb on + strand at 583.018 kb on - strand at 583.041 kb on - strand at 583.520 kb on - strand, within MMJJ_RS03100
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 580,402 - +1.3 580,434 - +2.3 580,435 + -0.7 580,436 - -1.1 580,436 - -3.1 580,437 + -0.7 580,437 + -0.6 580,437 + -1.7 580,437 + -1.6 580,437 + -0.3 580,437 + -1.5 580,437 + -1.5 580,442 - -1.6 580,442 - -0.7 580,442 - -1.8 580,446 - +0.6 580,482 + +0.8 580,489 - +2.0 580,510 + -1.7 580,541 - -0.5 580,559 + +0.2 580,588 + +0.9 580,588 + +1.2 580,588 + -1.4 580,635 - MMJJ_RS03090 0.13 +1.3 580,636 + MMJJ_RS03090 0.13 -0.0 580,672 + MMJJ_RS03090 0.19 +2.2 580,672 + MMJJ_RS03090 0.19 -1.2 580,692 - +0.3 580,693 + MMJJ_RS03090 0.23 -1.3 580,693 + MMJJ_RS03090 0.23 -0.7 580,693 + MMJJ_RS03090 0.23 -0.1 580,729 - MMJJ_RS03090 0.29 -2.0 580,729 - MMJJ_RS03090 0.29 +1.3 580,729 - MMJJ_RS03090 0.29 -1.2 580,729 - MMJJ_RS03090 0.29 +0.6 580,729 - MMJJ_RS03090 0.29 +1.2 580,729 - MMJJ_RS03090 0.29 -2.4 580,729 - MMJJ_RS03090 0.29 -0.9 580,730 + MMJJ_RS03090 0.29 +1.3 580,730 + MMJJ_RS03090 0.29 -2.9 580,730 + MMJJ_RS03090 0.29 -2.0 580,730 + MMJJ_RS03090 0.29 +3.7 580,730 + MMJJ_RS03090 0.29 -0.0 580,731 - MMJJ_RS03090 0.29 -0.4 580,731 - MMJJ_RS03090 0.29 -0.4 580,731 - MMJJ_RS03090 0.29 +1.0 580,731 - MMJJ_RS03090 0.29 -3.0 580,731 - MMJJ_RS03090 0.29 +0.3 580,731 - MMJJ_RS03090 0.29 +0.8 580,732 + MMJJ_RS03090 0.29 +0.2 580,732 + MMJJ_RS03090 0.29 -0.3 580,737 - MMJJ_RS03090 0.30 +0.3 580,737 - MMJJ_RS03090 0.30 +3.1 580,738 + MMJJ_RS03090 0.30 +1.0 580,738 + MMJJ_RS03090 0.30 -1.4 580,738 + MMJJ_RS03090 0.30 +1.8 580,738 + MMJJ_RS03090 0.30 +1.3 580,761 + MMJJ_RS03090 0.34 -1.9 580,777 - MMJJ_RS03090 0.37 +1.3 580,777 - MMJJ_RS03090 0.37 +1.3 580,778 + MMJJ_RS03090 0.37 +0.0 580,781 + MMJJ_RS03090 0.38 -3.3 580,781 + MMJJ_RS03090 0.38 -0.8 580,789 + MMJJ_RS03090 0.39 -1.2 580,789 + MMJJ_RS03090 0.39 +0.8 580,789 + MMJJ_RS03090 0.39 -1.3 580,789 + MMJJ_RS03090 0.39 -1.1 580,790 - MMJJ_RS03090 0.39 -0.9 580,790 - MMJJ_RS03090 0.39 -1.5 580,791 + MMJJ_RS03090 0.39 -0.2 580,791 + MMJJ_RS03090 0.39 +1.4 580,834 + MMJJ_RS03090 0.46 +4.7 580,834 + MMJJ_RS03090 0.46 -1.5 580,835 - MMJJ_RS03090 0.47 -1.0 580,843 - MMJJ_RS03090 0.48 +0.6 580,844 + MMJJ_RS03090 0.48 -2.2 580,874 - MMJJ_RS03090 0.53 -0.0 580,908 - MMJJ_RS03090 0.59 +0.1 580,909 + MMJJ_RS03090 0.59 -1.4 580,918 - MMJJ_RS03090 0.60 +1.8 580,918 - MMJJ_RS03090 0.60 +0.9 580,919 + MMJJ_RS03090 0.61 +0.8 580,922 + MMJJ_RS03090 0.61 +1.2 580,922 + MMJJ_RS03090 0.61 -2.5 580,933 - MMJJ_RS03090 0.63 -2.3 580,933 - MMJJ_RS03090 0.63 +0.7 581,015 - MMJJ_RS03090 0.77 -2.2 581,015 - MMJJ_RS03090 0.77 +1.7 581,016 + MMJJ_RS03090 0.77 -1.3 581,016 + MMJJ_RS03090 0.77 -1.3 581,028 + MMJJ_RS03090 0.79 -2.5 581,028 + MMJJ_RS03090 0.79 -1.5 581,031 - MMJJ_RS03090 0.79 +1.5 581,053 - MMJJ_RS03090 0.83 +1.6 581,053 - MMJJ_RS03090 0.83 +0.9 581,054 + MMJJ_RS03090 0.83 -1.7 581,054 + MMJJ_RS03090 0.83 -2.3 581,054 + MMJJ_RS03090 0.83 +1.5 581,058 + MMJJ_RS03090 0.84 +0.2 581,074 + MMJJ_RS03090 0.87 -1.3 581,074 + MMJJ_RS03090 0.87 +0.9 581,107 - +0.3 581,142 - -0.6 581,153 - -0.7 581,153 - -0.3 581,154 + +0.6 581,156 - +1.0 581,156 - +0.9 581,157 + -0.6 581,162 + -0.6 581,162 + -2.8 581,172 - -0.5 581,173 + -0.7 581,184 + -1.9 581,197 - +1.0 581,197 - -0.5 581,197 - +2.0 581,198 + +1.3 581,198 + +2.0 581,198 + +0.1 581,201 - +1.2 581,201 - +0.4 581,201 - -1.4 581,202 + +1.3 581,202 + -0.2 581,202 + -1.0 581,202 + -1.4 581,203 - -2.0 581,206 + +0.3 581,230 + -0.0 581,248 - -2.1 581,249 + -4.0 581,249 + -2.3 581,249 + -0.3 581,249 + -0.3 581,249 + +1.2 581,306 + -0.0 581,330 - +1.3 581,415 - MMJJ_RS03095 0.11 -1.9 582,140 - MMJJ_RS03095 0.55 -0.3 582,143 - MMJJ_RS03095 0.55 -2.3 582,335 + MMJJ_RS03095 0.67 -1.5 582,484 + MMJJ_RS03095 0.76 -0.3 582,487 - MMJJ_RS03095 0.76 +1.3 582,946 + -1.3 582,964 - -1.0 583,003 + -1.8 583,018 - -0.3 583,041 - -1.0 583,520 - MMJJ_RS03100 0.25 +0.0
Or see this region's nucleotide sequence