Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02860 and MMJJ_RS02865 are separated by 103 nucleotides MMJJ_RS02865 and MMJJ_RS02870 are separated by 115 nucleotides MMJJ_RS02870 and MMJJ_RS02875 are separated by 127 nucleotides
MMJJ_RS02860: MMJJ_RS02860 - site-specific integrase, at 539,832 to 540,380
_RS02860
MMJJ_RS02865: MMJJ_RS02865 - amino acid ABC transporter substrate-binding protein, at 540,484 to 541,230
_RS02865
MMJJ_RS02870: MMJJ_RS02870 - hypothetical protein, at 541,346 to 541,867
_RS02870
MMJJ_RS02875: MMJJ_RS02875 - site-2 protease family protein, at 541,995 to 543,008
_RS02875
Position (kb) 541
542 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 540.412 kb on - strand at 540.426 kb on + strand at 540.450 kb on + strand at 540.452 kb on - strand at 540.455 kb on + strand at 540.464 kb on - strand at 540.477 kb on - strand at 540.478 kb on + strand at 540.524 kb on - strand at 540.540 kb on - strand at 540.540 kb on - strand at 540.540 kb on - strand at 540.541 kb on + strand at 540.567 kb on - strand, within MMJJ_RS02865 at 540.712 kb on - strand, within MMJJ_RS02865 at 540.713 kb on + strand, within MMJJ_RS02865 at 540.814 kb on + strand, within MMJJ_RS02865 at 540.814 kb on + strand, within MMJJ_RS02865 at 540.923 kb on - strand, within MMJJ_RS02865 at 540.971 kb on - strand, within MMJJ_RS02865 at 540.972 kb on + strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.001 kb on + strand, within MMJJ_RS02865 at 541.069 kb on - strand, within MMJJ_RS02865 at 541.089 kb on + strand, within MMJJ_RS02865 at 541.089 kb on + strand, within MMJJ_RS02865 at 541.096 kb on + strand, within MMJJ_RS02865 at 541.096 kb on + strand, within MMJJ_RS02865 at 541.128 kb on + strand, within MMJJ_RS02865 at 541.149 kb on + strand, within MMJJ_RS02865 at 541.196 kb on + strand at 541.228 kb on - strand at 541.260 kb on - strand at 541.261 kb on + strand at 541.264 kb on + strand at 541.264 kb on + strand at 541.264 kb on + strand at 541.278 kb on + strand at 541.278 kb on + strand at 541.358 kb on - strand at 541.367 kb on - strand at 541.368 kb on + strand at 541.368 kb on + strand at 541.372 kb on - strand at 541.373 kb on + strand at 541.436 kb on + strand, within MMJJ_RS02870 at 541.454 kb on - strand, within MMJJ_RS02870 at 541.455 kb on + strand, within MMJJ_RS02870 at 541.518 kb on - strand, within MMJJ_RS02870 at 541.518 kb on - strand, within MMJJ_RS02870 at 541.565 kb on + strand, within MMJJ_RS02870 at 541.566 kb on - strand, within MMJJ_RS02870 at 541.567 kb on + strand, within MMJJ_RS02870 at 541.629 kb on + strand, within MMJJ_RS02870 at 541.720 kb on - strand, within MMJJ_RS02870 at 541.748 kb on - strand, within MMJJ_RS02870 at 541.751 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.793 kb on + strand, within MMJJ_RS02870 at 541.827 kb on - strand at 541.833 kb on - strand at 541.842 kb on - strand at 541.842 kb on - strand at 541.848 kb on + strand at 541.877 kb on - strand at 541.882 kb on - strand at 541.882 kb on - strand at 541.883 kb on + strand at 541.904 kb on + strand at 541.918 kb on - strand at 541.919 kb on + strand at 541.919 kb on + strand at 541.922 kb on - strand at 541.922 kb on - strand at 541.922 kb on - strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.926 kb on - strand at 541.926 kb on - strand at 541.927 kb on + strand at 541.927 kb on + strand at 541.927 kb on + strand at 541.943 kb on + strand at 541.946 kb on + strand at 541.948 kb on - strand at 541.948 kb on - strand at 541.949 kb on + strand at 541.952 kb on - strand at 541.953 kb on + strand at 541.965 kb on - strand at 541.965 kb on - strand at 541.965 kb on - strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.967 kb on - strand at 541.967 kb on - strand at 541.967 kb on - strand at 541.968 kb on + strand at 541.968 kb on + strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 542.045 kb on - strand at 542.048 kb on - strand at 542.132 kb on + strand, within MMJJ_RS02875 at 542.157 kb on - strand, within MMJJ_RS02875 at 542.157 kb on - strand, within MMJJ_RS02875 at 542.158 kb on + strand, within MMJJ_RS02875 at 542.158 kb on + strand, within MMJJ_RS02875 at 542.179 kb on - strand, within MMJJ_RS02875 at 542.302 kb on - strand, within MMJJ_RS02875 at 542.333 kb on - strand, within MMJJ_RS02875 at 542.381 kb on + strand, within MMJJ_RS02875 at 542.396 kb on - strand, within MMJJ_RS02875 at 542.435 kb on + strand, within MMJJ_RS02875 at 542.438 kb on - strand, within MMJJ_RS02875 at 542.472 kb on - strand, within MMJJ_RS02875 at 542.475 kb on + strand, within MMJJ_RS02875 at 542.476 kb on - strand, within MMJJ_RS02875 at 542.477 kb on + strand, within MMJJ_RS02875 at 542.522 kb on + strand, within MMJJ_RS02875 at 542.522 kb on + strand, within MMJJ_RS02875 at 542.557 kb on - strand, within MMJJ_RS02875 at 542.558 kb on + strand, within MMJJ_RS02875 at 542.558 kb on + strand, within MMJJ_RS02875 at 542.570 kb on + strand, within MMJJ_RS02875 at 542.578 kb on - strand, within MMJJ_RS02875 at 542.578 kb on - strand, within MMJJ_RS02875 at 542.579 kb on + strand, within MMJJ_RS02875 at 542.582 kb on - strand, within MMJJ_RS02875 at 542.582 kb on - strand, within MMJJ_RS02875 at 542.583 kb on + strand, within MMJJ_RS02875 at 542.587 kb on + strand, within MMJJ_RS02875 at 542.627 kb on - strand, within MMJJ_RS02875 at 542.627 kb on - strand, within MMJJ_RS02875 at 542.633 kb on + strand, within MMJJ_RS02875 at 542.633 kb on + strand, within MMJJ_RS02875 at 542.633 kb on + strand, within MMJJ_RS02875 at 542.633 kb on + strand, within MMJJ_RS02875 at 542.657 kb on - strand, within MMJJ_RS02875 at 542.658 kb on + strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.661 kb on + strand, within MMJJ_RS02875 at 542.661 kb on + strand, within MMJJ_RS02875 at 542.667 kb on + strand, within MMJJ_RS02875 at 542.667 kb on + strand, within MMJJ_RS02875 at 542.673 kb on + strand, within MMJJ_RS02875 at 542.680 kb on + strand, within MMJJ_RS02875 at 542.684 kb on + strand, within MMJJ_RS02875 at 542.711 kb on + strand, within MMJJ_RS02875 at 542.768 kb on - strand, within MMJJ_RS02875 at 542.783 kb on - strand, within MMJJ_RS02875 at 542.797 kb on - strand, within MMJJ_RS02875 at 542.804 kb on - strand, within MMJJ_RS02875 at 542.805 kb on + strand, within MMJJ_RS02875 at 542.805 kb on + strand, within MMJJ_RS02875 at 542.823 kb on + strand, within MMJJ_RS02875 at 542.836 kb on - strand, within MMJJ_RS02875
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 540,412 - +2.6 540,426 + -2.0 540,450 + +0.7 540,452 - +2.3 540,455 + +0.3 540,464 - -0.6 540,477 - -2.7 540,478 + -0.1 540,524 - +0.7 540,540 - +1.3 540,540 - -0.7 540,540 - -2.5 540,541 + -2.9 540,567 - MMJJ_RS02865 0.11 -2.2 540,712 - MMJJ_RS02865 0.31 -0.1 540,713 + MMJJ_RS02865 0.31 -2.0 540,814 + MMJJ_RS02865 0.44 +1.4 540,814 + MMJJ_RS02865 0.44 -2.8 540,923 - MMJJ_RS02865 0.59 -2.7 540,971 - MMJJ_RS02865 0.65 -0.5 540,972 + MMJJ_RS02865 0.65 -0.1 541,000 - MMJJ_RS02865 0.69 -1.1 541,000 - MMJJ_RS02865 0.69 +0.8 541,000 - MMJJ_RS02865 0.69 -1.2 541,000 - MMJJ_RS02865 0.69 -1.9 541,001 + MMJJ_RS02865 0.69 -1.7 541,069 - MMJJ_RS02865 0.78 +1.9 541,089 + MMJJ_RS02865 0.81 +0.8 541,089 + MMJJ_RS02865 0.81 +0.1 541,096 + MMJJ_RS02865 0.82 +0.7 541,096 + MMJJ_RS02865 0.82 -1.0 541,128 + MMJJ_RS02865 0.86 +0.1 541,149 + MMJJ_RS02865 0.89 -0.8 541,196 + +0.2 541,228 - -2.2 541,260 - -4.0 541,261 + +0.1 541,264 + -0.7 541,264 + +1.3 541,264 + -2.7 541,278 + +1.4 541,278 + +0.5 541,358 - -0.5 541,367 - -1.0 541,368 + +1.3 541,368 + +0.4 541,372 - -1.2 541,373 + -0.5 541,436 + MMJJ_RS02870 0.17 +0.4 541,454 - MMJJ_RS02870 0.21 -1.5 541,455 + MMJJ_RS02870 0.21 -0.3 541,518 - MMJJ_RS02870 0.33 +2.2 541,518 - MMJJ_RS02870 0.33 -1.7 541,565 + MMJJ_RS02870 0.42 +1.6 541,566 - MMJJ_RS02870 0.42 -1.8 541,567 + MMJJ_RS02870 0.42 -0.1 541,629 + MMJJ_RS02870 0.54 +2.8 541,720 - MMJJ_RS02870 0.72 -1.0 541,748 - MMJJ_RS02870 0.77 -2.3 541,751 + MMJJ_RS02870 0.78 -1.1 541,762 + MMJJ_RS02870 0.80 -0.8 541,762 + MMJJ_RS02870 0.80 +0.4 541,762 + MMJJ_RS02870 0.80 -0.9 541,762 + MMJJ_RS02870 0.80 +2.2 541,793 + MMJJ_RS02870 0.86 +4.7 541,827 - +0.7 541,833 - -1.5 541,842 - -0.0 541,842 - -2.5 541,848 + +1.9 541,877 - +1.9 541,882 - -1.5 541,882 - +0.0 541,883 + +1.6 541,904 + -0.6 541,918 - +1.7 541,919 + +0.1 541,919 + +1.6 541,922 - +0.1 541,922 - -0.9 541,922 - +0.1 541,923 + -0.5 541,923 + +0.5 541,923 + -1.9 541,923 + +2.0 541,923 + -1.1 541,923 + -2.5 541,923 + -0.2 541,926 - +0.0 541,926 - -1.9 541,927 + -2.4 541,927 + +1.3 541,927 + -1.7 541,943 + +2.7 541,946 + -4.2 541,948 - +3.4 541,948 - -0.5 541,949 + +0.8 541,952 - +0.3 541,953 + +0.3 541,965 - -2.4 541,965 - -0.7 541,965 - -1.2 541,966 + -1.5 541,966 + +0.7 541,966 + +0.1 541,966 + -3.0 541,966 + +1.2 541,966 + -2.2 541,967 - +0.3 541,967 - -0.3 541,967 - -1.5 541,968 + +1.6 541,968 + -0.6 541,970 - -1.5 541,970 - -1.4 541,970 - -2.0 541,970 - -0.7 541,971 + -2.6 541,971 + +1.6 541,971 + -1.1 541,971 + -0.3 541,971 + -1.4 542,045 - +1.0 542,048 - -3.1 542,132 + MMJJ_RS02875 0.14 +0.1 542,157 - MMJJ_RS02875 0.16 -1.6 542,157 - MMJJ_RS02875 0.16 +1.2 542,158 + MMJJ_RS02875 0.16 -2.4 542,158 + MMJJ_RS02875 0.16 -0.3 542,179 - MMJJ_RS02875 0.18 +1.3 542,302 - MMJJ_RS02875 0.30 +0.9 542,333 - MMJJ_RS02875 0.33 +2.3 542,381 + MMJJ_RS02875 0.38 +3.1 542,396 - MMJJ_RS02875 0.40 +0.3 542,435 + MMJJ_RS02875 0.43 -2.2 542,438 - MMJJ_RS02875 0.44 -2.0 542,472 - MMJJ_RS02875 0.47 +1.9 542,475 + MMJJ_RS02875 0.47 +2.5 542,476 - MMJJ_RS02875 0.47 -0.9 542,477 + MMJJ_RS02875 0.48 -1.0 542,522 + MMJJ_RS02875 0.52 -0.9 542,522 + MMJJ_RS02875 0.52 -2.0 542,557 - MMJJ_RS02875 0.55 -0.7 542,558 + MMJJ_RS02875 0.56 +2.9 542,558 + MMJJ_RS02875 0.56 +0.6 542,570 + MMJJ_RS02875 0.57 -0.2 542,578 - MMJJ_RS02875 0.57 +2.6 542,578 - MMJJ_RS02875 0.57 -2.4 542,579 + MMJJ_RS02875 0.58 -2.3 542,582 - MMJJ_RS02875 0.58 +2.6 542,582 - MMJJ_RS02875 0.58 +2.5 542,583 + MMJJ_RS02875 0.58 +0.3 542,587 + MMJJ_RS02875 0.58 +0.5 542,627 - MMJJ_RS02875 0.62 +2.5 542,627 - MMJJ_RS02875 0.62 +2.0 542,633 + MMJJ_RS02875 0.63 +2.1 542,633 + MMJJ_RS02875 0.63 +2.2 542,633 + MMJJ_RS02875 0.63 +3.1 542,633 + MMJJ_RS02875 0.63 -1.9 542,657 - MMJJ_RS02875 0.65 -1.9 542,658 + MMJJ_RS02875 0.65 +1.6 542,660 - MMJJ_RS02875 0.66 +0.2 542,660 - MMJJ_RS02875 0.66 +0.1 542,660 - MMJJ_RS02875 0.66 +2.8 542,660 - MMJJ_RS02875 0.66 -0.9 542,660 - MMJJ_RS02875 0.66 -2.2 542,660 - MMJJ_RS02875 0.66 +1.4 542,660 - MMJJ_RS02875 0.66 -2.2 542,661 + MMJJ_RS02875 0.66 -0.7 542,661 + MMJJ_RS02875 0.66 +1.7 542,667 + MMJJ_RS02875 0.66 +1.3 542,667 + MMJJ_RS02875 0.66 -2.4 542,673 + MMJJ_RS02875 0.67 -1.4 542,680 + MMJJ_RS02875 0.68 -0.3 542,684 + MMJJ_RS02875 0.68 -2.4 542,711 + MMJJ_RS02875 0.71 -1.0 542,768 - MMJJ_RS02875 0.76 -2.1 542,783 - MMJJ_RS02875 0.78 +3.7 542,797 - MMJJ_RS02875 0.79 +1.9 542,804 - MMJJ_RS02875 0.80 +1.3 542,805 + MMJJ_RS02875 0.80 +2.3 542,805 + MMJJ_RS02875 0.80 +1.9 542,823 + MMJJ_RS02875 0.82 +2.8 542,836 - MMJJ_RS02875 0.83 -1.7
Or see this region's nucleotide sequence