Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02165 and MMJJ_RS02170 are separated by 169 nucleotides MMJJ_RS02170 and MMJJ_RS02175 are separated by 9 nucleotides MMJJ_RS02175 and MMJJ_RS02180 are separated by 75 nucleotides
MMJJ_RS02165: MMJJ_RS02165 - ABC transporter substrate-binding protein, at 411,074 to 411,868
_RS02165
MMJJ_RS02170: MMJJ_RS02170 - 2-thiouracil desulfurase family protein, at 412,038 to 412,586
_RS02170
MMJJ_RS02175: MMJJ_RS02175 - imidazoleglycerol-phosphate dehydratase HisB, at 412,596 to 413,168
_RS02175
MMJJ_RS02180: MMJJ_RS02180 - DHH family phosphoesterase, at 413,244 to 414,539
_RS02180
Position (kb) 412
413
414 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 411.656 kb on - strand, within MMJJ_RS02165 at 411.657 kb on + strand, within MMJJ_RS02165 at 411.657 kb on + strand, within MMJJ_RS02165 at 411.735 kb on + strand, within MMJJ_RS02165 at 411.854 kb on - strand at 411.860 kb on + strand at 411.916 kb on - strand at 411.991 kb on + strand at 412.039 kb on - strand at 412.110 kb on - strand, within MMJJ_RS02170 at 412.149 kb on + strand, within MMJJ_RS02170 at 412.211 kb on - strand, within MMJJ_RS02170 at 412.211 kb on - strand, within MMJJ_RS02170 at 412.212 kb on + strand, within MMJJ_RS02170 at 412.212 kb on + strand, within MMJJ_RS02170 at 412.285 kb on + strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.294 kb on + strand, within MMJJ_RS02170 at 412.311 kb on - strand, within MMJJ_RS02170 at 412.314 kb on + strand, within MMJJ_RS02170 at 412.315 kb on - strand, within MMJJ_RS02170 at 412.316 kb on + strand, within MMJJ_RS02170 at 412.316 kb on + strand, within MMJJ_RS02170 at 412.333 kb on + strand, within MMJJ_RS02170 at 412.348 kb on + strand, within MMJJ_RS02170 at 412.396 kb on + strand, within MMJJ_RS02170 at 412.396 kb on + strand, within MMJJ_RS02170 at 412.413 kb on - strand, within MMJJ_RS02170 at 412.473 kb on - strand, within MMJJ_RS02170 at 412.478 kb on + strand, within MMJJ_RS02170 at 412.495 kb on + strand, within MMJJ_RS02170 at 412.543 kb on + strand at 412.717 kb on + strand at 413.196 kb on + strand at 413.196 kb on + strand at 413.198 kb on + strand at 413.200 kb on - strand at 413.200 kb on - strand at 413.200 kb on - strand at 413.201 kb on + strand at 413.222 kb on - strand at 413.223 kb on + strand at 413.246 kb on + strand at 413.541 kb on + strand, within MMJJ_RS02180 at 413.556 kb on - strand, within MMJJ_RS02180 at 413.711 kb on + strand, within MMJJ_RS02180 at 413.737 kb on + strand, within MMJJ_RS02180 at 413.820 kb on - strand, within MMJJ_RS02180 at 413.917 kb on - strand, within MMJJ_RS02180 at 413.920 kb on + strand, within MMJJ_RS02180 at 413.961 kb on + strand, within MMJJ_RS02180 at 414.004 kb on + strand, within MMJJ_RS02180 at 414.008 kb on - strand, within MMJJ_RS02180
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 411,656 - MMJJ_RS02165 0.73 -1.8 411,657 + MMJJ_RS02165 0.73 -2.0 411,657 + MMJJ_RS02165 0.73 +4.0 411,735 + MMJJ_RS02165 0.83 -1.8 411,854 - +0.2 411,860 + -0.2 411,916 - +2.2 411,991 + -0.1 412,039 - +0.5 412,110 - MMJJ_RS02170 0.13 -3.2 412,149 + MMJJ_RS02170 0.20 +2.6 412,211 - MMJJ_RS02170 0.32 -1.6 412,211 - MMJJ_RS02170 0.32 +1.8 412,212 + MMJJ_RS02170 0.32 -0.2 412,212 + MMJJ_RS02170 0.32 +0.5 412,285 + MMJJ_RS02170 0.45 +0.0 412,287 - MMJJ_RS02170 0.45 -1.9 412,287 - MMJJ_RS02170 0.45 -2.3 412,287 - MMJJ_RS02170 0.45 -1.2 412,288 + MMJJ_RS02170 0.46 -1.1 412,288 + MMJJ_RS02170 0.46 -2.9 412,288 + MMJJ_RS02170 0.46 -2.8 412,294 + MMJJ_RS02170 0.47 -0.3 412,311 - MMJJ_RS02170 0.50 -1.2 412,314 + MMJJ_RS02170 0.50 -1.3 412,315 - MMJJ_RS02170 0.50 +3.0 412,316 + MMJJ_RS02170 0.51 +6.6 412,316 + MMJJ_RS02170 0.51 -1.1 412,333 + MMJJ_RS02170 0.54 -1.3 412,348 + MMJJ_RS02170 0.56 -0.3 412,396 + MMJJ_RS02170 0.65 -1.6 412,396 + MMJJ_RS02170 0.65 +1.0 412,413 - MMJJ_RS02170 0.68 -1.9 412,473 - MMJJ_RS02170 0.79 +0.1 412,478 + MMJJ_RS02170 0.80 -2.0 412,495 + MMJJ_RS02170 0.83 +2.8 412,543 + +1.2 412,717 + -0.3 413,196 + -2.9 413,196 + -1.6 413,198 + -1.8 413,200 - -2.1 413,200 - -0.1 413,200 - +2.0 413,201 + -0.8 413,222 - +1.1 413,223 + -1.3 413,246 + -0.2 413,541 + MMJJ_RS02180 0.23 -1.1 413,556 - MMJJ_RS02180 0.24 +0.9 413,711 + MMJJ_RS02180 0.36 +0.7 413,737 + MMJJ_RS02180 0.38 -2.7 413,820 - MMJJ_RS02180 0.44 +0.1 413,917 - MMJJ_RS02180 0.52 -3.3 413,920 + MMJJ_RS02180 0.52 -0.3 413,961 + MMJJ_RS02180 0.55 +0.4 414,004 + MMJJ_RS02180 0.59 +0.2 414,008 - MMJJ_RS02180 0.59 +1.0
Or see this region's nucleotide sequence