Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02155 and MMJJ_RS02160 are separated by 77 nucleotides MMJJ_RS02160 and MMJJ_RS02165 are separated by 34 nucleotides MMJJ_RS02165 and MMJJ_RS02170 are separated by 169 nucleotides MMJJ_RS02170 and MMJJ_RS02175 are separated by 9 nucleotides
MMJJ_RS02155: MMJJ_RS02155 - DMT family transporter, at 409,412 to 410,302
_RS02155
MMJJ_RS02160: MMJJ_RS02160 - amino acid ABC transporter permease, at 410,380 to 411,039
_RS02160
MMJJ_RS02165: MMJJ_RS02165 - ABC transporter substrate-binding protein, at 411,074 to 411,868
_RS02165
MMJJ_RS02170: MMJJ_RS02170 - 2-thiouracil desulfurase family protein, at 412,038 to 412,586
_RS02170
MMJJ_RS02175: MMJJ_RS02175 - imidazoleglycerol-phosphate dehydratase HisB, at 412,596 to 413,168
_RS02175
Position (kb)
411
412 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 410.086 kb on + strand, within MMJJ_RS02155 at 410.150 kb on - strand, within MMJJ_RS02155 at 410.151 kb on + strand, within MMJJ_RS02155 at 410.275 kb on - strand at 410.276 kb on + strand at 410.305 kb on - strand at 410.331 kb on - strand at 410.351 kb on - strand at 410.352 kb on + strand at 410.352 kb on + strand at 410.352 kb on + strand at 410.355 kb on - strand at 410.356 kb on + strand at 410.356 kb on + strand at 410.370 kb on - strand at 410.371 kb on + strand at 410.374 kb on - strand at 410.438 kb on + strand at 410.457 kb on + strand, within MMJJ_RS02160 at 410.460 kb on - strand, within MMJJ_RS02160 at 410.460 kb on - strand, within MMJJ_RS02160 at 410.460 kb on - strand, within MMJJ_RS02160 at 410.461 kb on + strand, within MMJJ_RS02160 at 410.461 kb on + strand, within MMJJ_RS02160 at 410.474 kb on + strand, within MMJJ_RS02160 at 410.477 kb on - strand, within MMJJ_RS02160 at 410.477 kb on - strand, within MMJJ_RS02160 at 410.478 kb on + strand, within MMJJ_RS02160 at 410.508 kb on + strand, within MMJJ_RS02160 at 410.513 kb on + strand, within MMJJ_RS02160 at 410.555 kb on + strand, within MMJJ_RS02160 at 410.593 kb on - strand, within MMJJ_RS02160 at 410.611 kb on - strand, within MMJJ_RS02160 at 410.637 kb on - strand, within MMJJ_RS02160 at 410.638 kb on + strand, within MMJJ_RS02160 at 410.639 kb on - strand, within MMJJ_RS02160 at 410.646 kb on - strand, within MMJJ_RS02160 at 410.737 kb on + strand, within MMJJ_RS02160 at 410.794 kb on + strand, within MMJJ_RS02160 at 410.819 kb on - strand, within MMJJ_RS02160 at 410.820 kb on + strand, within MMJJ_RS02160 at 410.870 kb on - strand, within MMJJ_RS02160 at 410.871 kb on + strand, within MMJJ_RS02160 at 410.871 kb on + strand, within MMJJ_RS02160 at 410.878 kb on - strand, within MMJJ_RS02160 at 410.884 kb on - strand, within MMJJ_RS02160 at 410.885 kb on + strand, within MMJJ_RS02160 at 411.055 kb on + strand at 411.080 kb on - strand at 411.081 kb on + strand at 411.081 kb on + strand at 411.161 kb on - strand, within MMJJ_RS02165 at 411.162 kb on + strand, within MMJJ_RS02165 at 411.191 kb on - strand, within MMJJ_RS02165 at 411.260 kb on - strand, within MMJJ_RS02165 at 411.261 kb on + strand, within MMJJ_RS02165 at 411.261 kb on + strand, within MMJJ_RS02165 at 411.264 kb on - strand, within MMJJ_RS02165 at 411.264 kb on - strand, within MMJJ_RS02165 at 411.264 kb on - strand, within MMJJ_RS02165 at 411.265 kb on + strand, within MMJJ_RS02165 at 411.265 kb on + strand, within MMJJ_RS02165 at 411.265 kb on + strand, within MMJJ_RS02165 at 411.268 kb on - strand, within MMJJ_RS02165 at 411.268 kb on - strand, within MMJJ_RS02165 at 411.268 kb on - strand, within MMJJ_RS02165 at 411.331 kb on + strand, within MMJJ_RS02165 at 411.335 kb on + strand, within MMJJ_RS02165 at 411.336 kb on - strand, within MMJJ_RS02165 at 411.401 kb on - strand, within MMJJ_RS02165 at 411.411 kb on + strand, within MMJJ_RS02165 at 411.422 kb on - strand, within MMJJ_RS02165 at 411.471 kb on + strand, within MMJJ_RS02165 at 411.471 kb on + strand, within MMJJ_RS02165 at 411.549 kb on + strand, within MMJJ_RS02165 at 411.558 kb on - strand, within MMJJ_RS02165 at 411.559 kb on + strand, within MMJJ_RS02165 at 411.656 kb on - strand, within MMJJ_RS02165 at 411.657 kb on + strand, within MMJJ_RS02165 at 411.657 kb on + strand, within MMJJ_RS02165 at 411.735 kb on + strand, within MMJJ_RS02165 at 411.854 kb on - strand at 411.860 kb on + strand at 411.916 kb on - strand at 411.991 kb on + strand at 412.039 kb on - strand at 412.110 kb on - strand, within MMJJ_RS02170 at 412.149 kb on + strand, within MMJJ_RS02170 at 412.211 kb on - strand, within MMJJ_RS02170 at 412.211 kb on - strand, within MMJJ_RS02170 at 412.212 kb on + strand, within MMJJ_RS02170 at 412.212 kb on + strand, within MMJJ_RS02170 at 412.285 kb on + strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.294 kb on + strand, within MMJJ_RS02170 at 412.311 kb on - strand, within MMJJ_RS02170 at 412.314 kb on + strand, within MMJJ_RS02170 at 412.315 kb on - strand, within MMJJ_RS02170 at 412.316 kb on + strand, within MMJJ_RS02170 at 412.316 kb on + strand, within MMJJ_RS02170 at 412.333 kb on + strand, within MMJJ_RS02170 at 412.348 kb on + strand, within MMJJ_RS02170 at 412.396 kb on + strand, within MMJJ_RS02170 at 412.396 kb on + strand, within MMJJ_RS02170 at 412.413 kb on - strand, within MMJJ_RS02170 at 412.473 kb on - strand, within MMJJ_RS02170 at 412.478 kb on + strand, within MMJJ_RS02170 at 412.495 kb on + strand, within MMJJ_RS02170 at 412.543 kb on + strand at 412.717 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 410,086 + MMJJ_RS02155 0.76 +0.2 410,150 - MMJJ_RS02155 0.83 -2.1 410,151 + MMJJ_RS02155 0.83 -4.2 410,275 - +1.4 410,276 + +2.8 410,305 - -0.1 410,331 - -0.3 410,351 - -1.6 410,352 + +0.4 410,352 + +1.2 410,352 + +0.3 410,355 - +0.6 410,356 + -2.9 410,356 + -0.3 410,370 - -0.2 410,371 + +0.2 410,374 - +3.3 410,438 + -2.5 410,457 + MMJJ_RS02160 0.12 -0.8 410,460 - MMJJ_RS02160 0.12 -0.8 410,460 - MMJJ_RS02160 0.12 +0.8 410,460 - MMJJ_RS02160 0.12 -2.1 410,461 + MMJJ_RS02160 0.12 -0.8 410,461 + MMJJ_RS02160 0.12 +1.4 410,474 + MMJJ_RS02160 0.14 +2.2 410,477 - MMJJ_RS02160 0.15 +1.2 410,477 - MMJJ_RS02160 0.15 -0.3 410,478 + MMJJ_RS02160 0.15 +0.7 410,508 + MMJJ_RS02160 0.19 +0.5 410,513 + MMJJ_RS02160 0.20 -2.2 410,555 + MMJJ_RS02160 0.27 -1.6 410,593 - MMJJ_RS02160 0.32 -1.6 410,611 - MMJJ_RS02160 0.35 -3.4 410,637 - MMJJ_RS02160 0.39 +0.8 410,638 + MMJJ_RS02160 0.39 +2.9 410,639 - MMJJ_RS02160 0.39 +0.5 410,646 - MMJJ_RS02160 0.40 +1.6 410,737 + MMJJ_RS02160 0.54 -0.3 410,794 + MMJJ_RS02160 0.63 -0.3 410,819 - MMJJ_RS02160 0.67 +2.3 410,820 + MMJJ_RS02160 0.67 -1.8 410,870 - MMJJ_RS02160 0.74 -1.1 410,871 + MMJJ_RS02160 0.74 -0.9 410,871 + MMJJ_RS02160 0.74 +0.7 410,878 - MMJJ_RS02160 0.75 +2.8 410,884 - MMJJ_RS02160 0.76 -1.7 410,885 + MMJJ_RS02160 0.77 -0.2 411,055 + +0.0 411,080 - -0.1 411,081 + +1.1 411,081 + -1.1 411,161 - MMJJ_RS02165 0.11 -2.7 411,162 + MMJJ_RS02165 0.11 -1.8 411,191 - MMJJ_RS02165 0.15 -1.2 411,260 - MMJJ_RS02165 0.23 -1.6 411,261 + MMJJ_RS02165 0.24 -0.4 411,261 + MMJJ_RS02165 0.24 +0.9 411,264 - MMJJ_RS02165 0.24 +1.6 411,264 - MMJJ_RS02165 0.24 +1.2 411,264 - MMJJ_RS02165 0.24 +1.3 411,265 + MMJJ_RS02165 0.24 +0.8 411,265 + MMJJ_RS02165 0.24 +0.3 411,265 + MMJJ_RS02165 0.24 +0.7 411,268 - MMJJ_RS02165 0.24 +1.2 411,268 - MMJJ_RS02165 0.24 -2.6 411,268 - MMJJ_RS02165 0.24 +1.9 411,331 + MMJJ_RS02165 0.32 -1.2 411,335 + MMJJ_RS02165 0.33 +1.6 411,336 - MMJJ_RS02165 0.33 +1.0 411,401 - MMJJ_RS02165 0.41 +1.0 411,411 + MMJJ_RS02165 0.42 -1.8 411,422 - MMJJ_RS02165 0.44 -0.6 411,471 + MMJJ_RS02165 0.50 -1.6 411,471 + MMJJ_RS02165 0.50 -2.1 411,549 + MMJJ_RS02165 0.60 -2.6 411,558 - MMJJ_RS02165 0.61 +2.1 411,559 + MMJJ_RS02165 0.61 -2.6 411,656 - MMJJ_RS02165 0.73 -1.8 411,657 + MMJJ_RS02165 0.73 -2.0 411,657 + MMJJ_RS02165 0.73 +4.0 411,735 + MMJJ_RS02165 0.83 -1.8 411,854 - +0.2 411,860 + -0.2 411,916 - +2.2 411,991 + -0.1 412,039 - +0.5 412,110 - MMJJ_RS02170 0.13 -3.2 412,149 + MMJJ_RS02170 0.20 +2.6 412,211 - MMJJ_RS02170 0.32 -1.6 412,211 - MMJJ_RS02170 0.32 +1.8 412,212 + MMJJ_RS02170 0.32 -0.2 412,212 + MMJJ_RS02170 0.32 +0.5 412,285 + MMJJ_RS02170 0.45 +0.0 412,287 - MMJJ_RS02170 0.45 -1.9 412,287 - MMJJ_RS02170 0.45 -2.3 412,287 - MMJJ_RS02170 0.45 -1.2 412,288 + MMJJ_RS02170 0.46 -1.1 412,288 + MMJJ_RS02170 0.46 -2.9 412,288 + MMJJ_RS02170 0.46 -2.8 412,294 + MMJJ_RS02170 0.47 -0.3 412,311 - MMJJ_RS02170 0.50 -1.2 412,314 + MMJJ_RS02170 0.50 -1.3 412,315 - MMJJ_RS02170 0.50 +3.0 412,316 + MMJJ_RS02170 0.51 +6.6 412,316 + MMJJ_RS02170 0.51 -1.1 412,333 + MMJJ_RS02170 0.54 -1.3 412,348 + MMJJ_RS02170 0.56 -0.3 412,396 + MMJJ_RS02170 0.65 -1.6 412,396 + MMJJ_RS02170 0.65 +1.0 412,413 - MMJJ_RS02170 0.68 -1.9 412,473 - MMJJ_RS02170 0.79 +0.1 412,478 + MMJJ_RS02170 0.80 -2.0 412,495 + MMJJ_RS02170 0.83 +2.8 412,543 + +1.2 412,717 + -0.3
Or see this region's nucleotide sequence