Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02120 and MMJJ_RS02125 are separated by 118 nucleotides MMJJ_RS02125 and MMJJ_RS02130 are separated by 9 nucleotides MMJJ_RS02130 and MMJJ_RS02135 are separated by 1 nucleotides MMJJ_RS02135 and MMJJ_RS02140 overlap by 4 nucleotides MMJJ_RS02140 and MMJJ_RS02145 are separated by 43 nucleotides
MMJJ_RS02120: MMJJ_RS02120 - DJ-1/PfpI family protein, at 404,820 to 405,335
_RS02120
MMJJ_RS02125: MMJJ_RS02125 - hypothetical protein, at 405,454 to 405,750
_RS02125
MMJJ_RS02130: MMJJ_RS02130 - UPF0146 family protein, at 405,760 to 406,173
_RS02130
MMJJ_RS02135: MMJJ_RS02135 - TatD family hydrolase, at 406,175 to 407,017
_RS02135
MMJJ_RS02140: MMJJ_RS02140 - preflagellin peptidase FlaK, at 407,014 to 407,706
_RS02140
MMJJ_RS02145: MMJJ_RS02145 - class I SAM-dependent methyltransferase, at 407,750 to 408,352
_RS02145
Position (kb) 406
407
408 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 405.325 kb on + strand at 405.361 kb on + strand at 405.361 kb on + strand at 405.364 kb on - strand at 405.364 kb on - strand at 405.365 kb on + strand at 405.365 kb on + strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.372 kb on - strand at 405.372 kb on - strand at 405.373 kb on + strand at 405.373 kb on + strand at 405.374 kb on - strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.379 kb on - strand at 405.385 kb on + strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.426 kb on - strand at 405.427 kb on + strand at 405.442 kb on + strand at 405.513 kb on - strand, within MMJJ_RS02125 at 405.513 kb on - strand, within MMJJ_RS02125 at 405.513 kb on - strand, within MMJJ_RS02125 at 405.514 kb on + strand, within MMJJ_RS02125 at 405.583 kb on - strand, within MMJJ_RS02125 at 405.594 kb on - strand, within MMJJ_RS02125 at 405.749 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.782 kb on - strand at 405.818 kb on - strand at 405.903 kb on - strand, within MMJJ_RS02130 at 406.172 kb on + strand at 406.231 kb on + strand at 406.294 kb on - strand, within MMJJ_RS02135 at 406.295 kb on + strand, within MMJJ_RS02135 at 406.304 kb on + strand, within MMJJ_RS02135 at 406.315 kb on - strand, within MMJJ_RS02135 at 406.315 kb on - strand, within MMJJ_RS02135 at 406.316 kb on + strand, within MMJJ_RS02135 at 406.357 kb on + strand, within MMJJ_RS02135 at 406.369 kb on + strand, within MMJJ_RS02135 at 406.476 kb on - strand, within MMJJ_RS02135 at 406.476 kb on - strand, within MMJJ_RS02135 at 406.476 kb on - strand, within MMJJ_RS02135 at 406.477 kb on + strand, within MMJJ_RS02135 at 406.477 kb on + strand, within MMJJ_RS02135 at 406.477 kb on + strand, within MMJJ_RS02135 at 406.477 kb on + strand, within MMJJ_RS02135 at 406.501 kb on - strand, within MMJJ_RS02135 at 406.501 kb on - strand, within MMJJ_RS02135 at 406.510 kb on + strand, within MMJJ_RS02135 at 406.510 kb on + strand, within MMJJ_RS02135 at 406.575 kb on - strand, within MMJJ_RS02135 at 406.605 kb on + strand, within MMJJ_RS02135 at 406.628 kb on + strand, within MMJJ_RS02135 at 406.758 kb on - strand, within MMJJ_RS02135 at 406.856 kb on - strand, within MMJJ_RS02135 at 407.039 kb on + strand at 407.039 kb on + strand at 407.065 kb on - strand at 407.066 kb on + strand at 407.066 kb on + strand at 407.069 kb on - strand at 407.069 kb on - strand at 407.069 kb on - strand at 407.069 kb on - strand at 407.070 kb on + strand at 407.070 kb on + strand at 407.070 kb on + strand at 407.070 kb on + strand at 407.074 kb on - strand at 407.075 kb on + strand at 407.080 kb on - strand at 407.091 kb on - strand, within MMJJ_RS02140 at 407.110 kb on + strand, within MMJJ_RS02140 at 407.117 kb on + strand, within MMJJ_RS02140 at 407.135 kb on - strand, within MMJJ_RS02140 at 407.136 kb on + strand, within MMJJ_RS02140 at 407.381 kb on - strand, within MMJJ_RS02140 at 407.382 kb on + strand, within MMJJ_RS02140 at 407.414 kb on - strand, within MMJJ_RS02140 at 407.414 kb on - strand, within MMJJ_RS02140 at 407.414 kb on - strand, within MMJJ_RS02140 at 407.414 kb on - strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.492 kb on + strand, within MMJJ_RS02140 at 407.502 kb on - strand, within MMJJ_RS02140 at 407.504 kb on - strand, within MMJJ_RS02140 at 407.627 kb on - strand, within MMJJ_RS02140 at 407.628 kb on + strand, within MMJJ_RS02140 at 407.628 kb on + strand, within MMJJ_RS02140 at 407.668 kb on - strand at 407.684 kb on + strand at 407.697 kb on + strand at 407.697 kb on + strand at 407.725 kb on - strand at 407.730 kb on + strand at 407.730 kb on + strand at 407.730 kb on + strand at 407.741 kb on + strand at 407.799 kb on + strand at 407.804 kb on + strand at 407.837 kb on + strand, within MMJJ_RS02145 at 407.838 kb on - strand, within MMJJ_RS02145 at 407.843 kb on + strand, within MMJJ_RS02145 at 407.903 kb on - strand, within MMJJ_RS02145 at 407.940 kb on + strand, within MMJJ_RS02145 at 407.958 kb on + strand, within MMJJ_RS02145 at 407.974 kb on - strand, within MMJJ_RS02145 at 407.979 kb on + strand, within MMJJ_RS02145 at 407.979 kb on + strand, within MMJJ_RS02145 at 408.000 kb on + strand, within MMJJ_RS02145 at 408.012 kb on + strand, within MMJJ_RS02145 at 408.012 kb on + strand, within MMJJ_RS02145
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 405,325 + +0.9 405,361 + +1.2 405,361 + +2.1 405,364 - -2.7 405,364 - -1.1 405,365 + +4.4 405,365 + -0.8 405,370 - +0.8 405,370 - -2.6 405,370 - -2.6 405,370 - +0.8 405,370 - +2.0 405,370 - +3.5 405,370 - -1.3 405,370 - -1.9 405,370 - -2.2 405,370 - -1.6 405,370 - -1.8 405,370 - -0.3 405,370 - +1.1 405,371 + -2.0 405,371 + -0.3 405,371 + +2.7 405,371 + -2.7 405,371 + +1.3 405,371 + -0.4 405,371 + +2.1 405,371 + +0.8 405,371 + +1.1 405,371 + +2.7 405,371 + -0.8 405,371 + -1.1 405,371 + -2.2 405,371 + +0.6 405,371 + -0.3 405,371 + -1.8 405,371 + +3.9 405,371 + -0.5 405,372 - -1.9 405,372 - -3.1 405,373 + -2.2 405,373 + +2.5 405,374 - -0.7 405,375 + +1.7 405,375 + +0.4 405,375 + -2.7 405,375 + -0.9 405,379 - -2.6 405,385 + +1.2 405,398 - +1.4 405,398 - +0.7 405,398 - -0.1 405,398 - +1.3 405,426 - +3.5 405,427 + -0.6 405,442 + -0.5 405,513 - MMJJ_RS02125 0.20 +3.3 405,513 - MMJJ_RS02125 0.20 -1.8 405,513 - MMJJ_RS02125 0.20 +1.5 405,514 + MMJJ_RS02125 0.20 +0.9 405,583 - MMJJ_RS02125 0.43 -1.5 405,594 - MMJJ_RS02125 0.47 -0.8 405,749 + +0.2 405,779 + -3.0 405,779 + -0.8 405,782 - +0.2 405,818 - +0.2 405,903 - MMJJ_RS02130 0.35 -0.3 406,172 + +1.0 406,231 + -2.2 406,294 - MMJJ_RS02135 0.14 +2.5 406,295 + MMJJ_RS02135 0.14 +1.4 406,304 + MMJJ_RS02135 0.15 +2.7 406,315 - MMJJ_RS02135 0.17 -0.2 406,315 - MMJJ_RS02135 0.17 +1.9 406,316 + MMJJ_RS02135 0.17 -1.2 406,357 + MMJJ_RS02135 0.22 -1.8 406,369 + MMJJ_RS02135 0.23 +0.4 406,476 - MMJJ_RS02135 0.36 -2.9 406,476 - MMJJ_RS02135 0.36 +2.9 406,476 - MMJJ_RS02135 0.36 +1.9 406,477 + MMJJ_RS02135 0.36 -2.1 406,477 + MMJJ_RS02135 0.36 +1.1 406,477 + MMJJ_RS02135 0.36 -2.3 406,477 + MMJJ_RS02135 0.36 +0.3 406,501 - MMJJ_RS02135 0.39 -0.5 406,501 - MMJJ_RS02135 0.39 +1.0 406,510 + MMJJ_RS02135 0.40 +4.3 406,510 + MMJJ_RS02135 0.40 -2.8 406,575 - MMJJ_RS02135 0.47 -4.3 406,605 + MMJJ_RS02135 0.51 -1.6 406,628 + MMJJ_RS02135 0.54 -0.3 406,758 - MMJJ_RS02135 0.69 +0.3 406,856 - MMJJ_RS02135 0.81 +0.5 407,039 + -2.1 407,039 + -2.7 407,065 - +0.7 407,066 + +1.8 407,066 + +0.8 407,069 - -2.0 407,069 - -0.3 407,069 - -2.2 407,069 - -1.3 407,070 + -0.3 407,070 + -0.6 407,070 + -2.3 407,070 + -0.3 407,074 - +0.6 407,075 + +0.2 407,080 - +2.1 407,091 - MMJJ_RS02140 0.11 -0.5 407,110 + MMJJ_RS02140 0.14 -1.7 407,117 + MMJJ_RS02140 0.15 +5.6 407,135 - MMJJ_RS02140 0.17 +1.2 407,136 + MMJJ_RS02140 0.18 -2.8 407,381 - MMJJ_RS02140 0.53 +2.2 407,382 + MMJJ_RS02140 0.53 -0.1 407,414 - MMJJ_RS02140 0.58 -2.3 407,414 - MMJJ_RS02140 0.58 +2.4 407,414 - MMJJ_RS02140 0.58 -2.6 407,414 - MMJJ_RS02140 0.58 +0.2 407,415 + MMJJ_RS02140 0.58 -0.5 407,415 + MMJJ_RS02140 0.58 -0.4 407,415 + MMJJ_RS02140 0.58 -0.1 407,415 + MMJJ_RS02140 0.58 +1.2 407,415 + MMJJ_RS02140 0.58 -0.5 407,415 + MMJJ_RS02140 0.58 +1.2 407,492 + MMJJ_RS02140 0.69 -0.2 407,502 - MMJJ_RS02140 0.70 -0.1 407,504 - MMJJ_RS02140 0.71 +1.6 407,627 - MMJJ_RS02140 0.88 -0.0 407,628 + MMJJ_RS02140 0.89 -0.3 407,628 + MMJJ_RS02140 0.89 +2.8 407,668 - +0.6 407,684 + +1.1 407,697 + +0.2 407,697 + +0.1 407,725 - +2.5 407,730 + +0.8 407,730 + -3.0 407,730 + +0.9 407,741 + -1.6 407,799 + -2.6 407,804 + +2.9 407,837 + MMJJ_RS02145 0.14 +0.2 407,838 - MMJJ_RS02145 0.15 -1.2 407,843 + MMJJ_RS02145 0.15 -1.3 407,903 - MMJJ_RS02145 0.25 +0.6 407,940 + MMJJ_RS02145 0.32 +0.2 407,958 + MMJJ_RS02145 0.34 +3.8 407,974 - MMJJ_RS02145 0.37 -1.1 407,979 + MMJJ_RS02145 0.38 +1.4 407,979 + MMJJ_RS02145 0.38 -1.5 408,000 + MMJJ_RS02145 0.41 -1.6 408,012 + MMJJ_RS02145 0.43 -2.7 408,012 + MMJJ_RS02145 0.43 +0.2
Or see this region's nucleotide sequence