Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01860 and MMJJ_RS01865 are separated by 147 nucleotides MMJJ_RS01865 and MMJJ_RS01870 are separated by 135 nucleotides
MMJJ_RS01860: MMJJ_RS01860 - tRNA guanosine(15) transglycosylase TgtA, at 356,203 to 358,152
_RS01860
MMJJ_RS01865: MMJJ_RS01865 - peptidyl-tRNA hydrolase Pth2, at 358,300 to 358,650
_RS01865
MMJJ_RS01870: MMJJ_RS01870 - methanogenesis marker 15 protein, at 358,786 to 360,021
_RS01870
Position (kb)
358
359 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 357.418 kb on - strand, within MMJJ_RS01860 at 357.451 kb on - strand, within MMJJ_RS01860 at 357.568 kb on - strand, within MMJJ_RS01860 at 357.568 kb on - strand, within MMJJ_RS01860 at 357.585 kb on + strand, within MMJJ_RS01860 at 357.588 kb on - strand, within MMJJ_RS01860 at 357.609 kb on - strand, within MMJJ_RS01860 at 357.679 kb on + strand at 357.703 kb on + strand, within MMJJ_RS01860 at 357.723 kb on - strand, within MMJJ_RS01860 at 357.724 kb on + strand, within MMJJ_RS01860 at 357.736 kb on - strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.783 kb on - strand, within MMJJ_RS01860 at 357.854 kb on + strand, within MMJJ_RS01860 at 357.854 kb on + strand, within MMJJ_RS01860 at 357.971 kb on - strand at 357.972 kb on + strand at 357.977 kb on + strand at 357.977 kb on + strand at 357.995 kb on + strand at 358.019 kb on + strand at 358.060 kb on - strand at 358.109 kb on + strand at 358.120 kb on + strand at 358.163 kb on - strand at 358.164 kb on + strand at 358.164 kb on + strand at 358.168 kb on - strand at 358.168 kb on - strand at 358.176 kb on + strand at 358.176 kb on + strand at 358.176 kb on + strand at 358.177 kb on - strand at 358.177 kb on - strand at 358.178 kb on + strand at 358.184 kb on - strand at 358.184 kb on - strand at 358.207 kb on - strand at 358.207 kb on - strand at 358.220 kb on - strand at 358.225 kb on - strand at 358.226 kb on + strand at 358.232 kb on - strand at 358.232 kb on - strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.236 kb on - strand at 358.236 kb on - strand at 358.239 kb on - strand at 358.239 kb on - strand at 358.239 kb on - strand at 358.240 kb on + strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.245 kb on - strand at 358.245 kb on - strand at 358.246 kb on + strand at 358.246 kb on + strand at 358.248 kb on - strand at 358.248 kb on - strand at 358.248 kb on - strand at 358.249 kb on + strand at 358.249 kb on + strand at 358.255 kb on - strand at 358.255 kb on - strand at 358.256 kb on + strand at 358.258 kb on + strand at 358.320 kb on - strand at 358.335 kb on + strand, within MMJJ_RS01865 at 358.335 kb on + strand, within MMJJ_RS01865 at 358.335 kb on + strand, within MMJJ_RS01865 at 358.410 kb on + strand, within MMJJ_RS01865 at 358.410 kb on + strand, within MMJJ_RS01865 at 358.470 kb on + strand, within MMJJ_RS01865 at 358.497 kb on + strand, within MMJJ_RS01865 at 358.535 kb on - strand, within MMJJ_RS01865 at 358.536 kb on + strand, within MMJJ_RS01865 at 358.648 kb on - strand at 358.649 kb on + strand at 358.656 kb on + strand at 358.672 kb on - strand at 358.690 kb on + strand at 358.690 kb on + strand at 358.692 kb on - strand at 358.692 kb on - strand at 358.692 kb on - strand at 358.693 kb on + strand at 358.694 kb on - strand at 358.694 kb on - strand at 358.694 kb on - strand at 358.695 kb on + strand at 358.699 kb on + strand at 358.716 kb on - strand at 358.722 kb on + strand at 358.722 kb on + strand at 358.883 kb on - strand at 359.061 kb on + strand at 359.082 kb on + strand, within MMJJ_RS01870 at 359.528 kb on - strand, within MMJJ_RS01870 at 359.528 kb on - strand, within MMJJ_RS01870
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 357,418 - MMJJ_RS01860 0.62 -0.4 357,451 - MMJJ_RS01860 0.64 +2.1 357,568 - MMJJ_RS01860 0.70 +3.3 357,568 - MMJJ_RS01860 0.70 -2.2 357,585 + MMJJ_RS01860 0.71 -1.9 357,588 - MMJJ_RS01860 0.71 -1.4 357,609 - MMJJ_RS01860 0.72 +2.6 357,679 + +0.1 357,703 + MMJJ_RS01860 0.77 -1.3 357,723 - MMJJ_RS01860 0.78 -0.6 357,724 + MMJJ_RS01860 0.78 -3.6 357,736 - MMJJ_RS01860 0.79 -2.3 357,737 + MMJJ_RS01860 0.79 -2.6 357,737 + MMJJ_RS01860 0.79 -2.0 357,737 + MMJJ_RS01860 0.79 -2.2 357,783 - MMJJ_RS01860 0.81 -1.8 357,854 + MMJJ_RS01860 0.85 +1.7 357,854 + MMJJ_RS01860 0.85 -0.4 357,971 - +1.5 357,972 + +1.1 357,977 + -1.9 357,977 + +3.3 357,995 + -1.2 358,019 + -1.2 358,060 - -3.4 358,109 + -0.2 358,120 + +1.9 358,163 - +2.0 358,164 + +2.0 358,164 + +4.2 358,168 - -1.2 358,168 - -0.6 358,176 + +1.5 358,176 + -2.1 358,176 + -3.9 358,177 - -2.5 358,177 - +1.0 358,178 + +2.2 358,184 - -1.7 358,184 - -0.7 358,207 - -2.9 358,207 - +1.1 358,220 - -1.0 358,225 - +2.4 358,226 + -0.4 358,232 - +1.6 358,232 - -1.5 358,233 + -1.4 358,233 + -1.7 358,233 + -3.1 358,233 + -2.2 358,236 - -2.4 358,236 - -0.4 358,239 - -0.9 358,239 - +3.2 358,239 - -1.0 358,240 + +4.0 358,243 - -2.0 358,243 - -1.4 358,243 - +0.1 358,243 - -2.7 358,243 - -2.8 358,244 + -1.6 358,244 + +1.5 358,244 + -2.4 358,244 + +0.7 358,244 + -0.3 358,244 + +1.9 358,245 - -2.2 358,245 - -2.0 358,246 + +1.5 358,246 + +1.0 358,248 - -3.2 358,248 - +0.1 358,248 - -0.1 358,249 + -3.8 358,249 + -1.2 358,255 - -4.1 358,255 - -2.9 358,256 + +1.8 358,258 + +2.2 358,320 - -0.7 358,335 + MMJJ_RS01865 0.10 -1.4 358,335 + MMJJ_RS01865 0.10 -2.7 358,335 + MMJJ_RS01865 0.10 -1.2 358,410 + MMJJ_RS01865 0.31 -1.2 358,410 + MMJJ_RS01865 0.31 -2.2 358,470 + MMJJ_RS01865 0.48 -2.2 358,497 + MMJJ_RS01865 0.56 -2.8 358,535 - MMJJ_RS01865 0.67 +1.4 358,536 + MMJJ_RS01865 0.67 -2.2 358,648 - -2.6 358,649 + -3.3 358,656 + +1.4 358,672 - +0.6 358,690 + +0.4 358,690 + -1.3 358,692 - -0.4 358,692 - +2.6 358,692 - +2.5 358,693 + -0.9 358,694 - -0.7 358,694 - +2.0 358,694 - +1.9 358,695 + +4.8 358,699 + -3.2 358,716 - -1.2 358,722 + -0.9 358,722 + -1.4 358,883 - -1.7 359,061 + +1.2 359,082 + MMJJ_RS01870 0.24 -1.4 359,528 - MMJJ_RS01870 0.60 +0.2 359,528 - MMJJ_RS01870 0.60 -1.2
Or see this region's nucleotide sequence