Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01820 and MMJJ_RS01825 are separated by 105 nucleotides MMJJ_RS01825 and MMJJ_RS01830 are separated by 46 nucleotides MMJJ_RS01830 and MMJJ_RS01835 are separated by 128 nucleotides
MMJJ_RS01820: MMJJ_RS01820 - MJ1244 family protein, at 349,332 to 349,661
_RS01820
MMJJ_RS01825: MMJJ_RS01825 - DNA repair and recombination protein RadB, at 349,767 to 350,417
_RS01825
MMJJ_RS01830: MMJJ_RS01830 - tRNA(Ile)(2)-agmatinylcytidine synthase, at 350,464 to 351,738
_RS01830
MMJJ_RS01835: MMJJ_RS01835 - tRNA pseudouridine(13) synthase TruD, at 351,867 to 353,159
_RS01835
Position (kb)
350
351
352 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 349.480 kb on + strand, within MMJJ_RS01820 at 349.677 kb on - strand at 349.677 kb on - strand at 349.677 kb on - strand at 349.696 kb on - strand at 349.696 kb on - strand at 349.696 kb on - strand at 349.712 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.714 kb on - strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.715 kb on + strand at 349.716 kb on - strand at 349.718 kb on - strand at 349.719 kb on + strand at 349.751 kb on + strand at 349.781 kb on - strand at 349.809 kb on - strand at 349.863 kb on + strand, within MMJJ_RS01825 at 349.863 kb on + strand, within MMJJ_RS01825 at 349.921 kb on + strand, within MMJJ_RS01825 at 349.954 kb on + strand, within MMJJ_RS01825 at 349.954 kb on + strand, within MMJJ_RS01825 at 349.976 kb on - strand, within MMJJ_RS01825 at 350.055 kb on + strand, within MMJJ_RS01825 at 350.093 kb on + strand, within MMJJ_RS01825 at 350.120 kb on - strand, within MMJJ_RS01825 at 350.120 kb on - strand, within MMJJ_RS01825 at 350.121 kb on + strand, within MMJJ_RS01825 at 350.121 kb on + strand, within MMJJ_RS01825 at 350.211 kb on - strand, within MMJJ_RS01825 at 350.334 kb on + strand, within MMJJ_RS01825 at 350.373 kb on - strand at 350.417 kb on - strand at 350.418 kb on + strand at 350.444 kb on + strand at 350.449 kb on - strand at 350.461 kb on + strand at 350.461 kb on + strand at 350.465 kb on - strand at 350.692 kb on - strand, within MMJJ_RS01830 at 350.803 kb on + strand, within MMJJ_RS01830 at 350.928 kb on - strand, within MMJJ_RS01830 at 351.034 kb on - strand, within MMJJ_RS01830 at 351.151 kb on + strand, within MMJJ_RS01830 at 351.248 kb on - strand, within MMJJ_RS01830 at 351.253 kb on + strand, within MMJJ_RS01830 at 351.400 kb on - strand, within MMJJ_RS01830 at 351.569 kb on + strand, within MMJJ_RS01830 at 351.675 kb on - strand at 351.686 kb on - strand at 351.708 kb on - strand at 351.734 kb on - strand at 351.750 kb on + strand at 351.808 kb on - strand at 351.808 kb on - strand at 351.809 kb on + strand at 351.809 kb on + strand at 351.812 kb on - strand at 351.813 kb on + strand at 351.813 kb on + strand at 351.813 kb on + strand at 351.813 kb on + strand at 351.813 kb on + strand at 351.817 kb on + strand at 351.835 kb on + strand at 351.837 kb on + strand at 351.839 kb on + strand at 351.839 kb on + strand at 351.839 kb on + strand at 351.839 kb on + strand at 351.863 kb on - strand at 351.881 kb on - strand at 351.916 kb on - strand at 351.916 kb on - strand at 352.016 kb on + strand, within MMJJ_RS01835 at 352.016 kb on + strand, within MMJJ_RS01835 at 352.016 kb on + strand, within MMJJ_RS01835 at 352.079 kb on + strand, within MMJJ_RS01835 at 352.099 kb on - strand, within MMJJ_RS01835 at 352.202 kb on + strand, within MMJJ_RS01835 at 352.213 kb on - strand, within MMJJ_RS01835 at 352.213 kb on - strand, within MMJJ_RS01835 at 352.214 kb on + strand, within MMJJ_RS01835 at 352.214 kb on + strand, within MMJJ_RS01835 at 352.214 kb on + strand, within MMJJ_RS01835 at 352.277 kb on + strand, within MMJJ_RS01835 at 352.280 kb on - strand, within MMJJ_RS01835 at 352.331 kb on - strand, within MMJJ_RS01835 at 352.344 kb on + strand, within MMJJ_RS01835 at 352.344 kb on + strand, within MMJJ_RS01835 at 352.432 kb on - strand, within MMJJ_RS01835 at 352.472 kb on + strand, within MMJJ_RS01835 at 352.482 kb on - strand, within MMJJ_RS01835 at 352.482 kb on - strand, within MMJJ_RS01835 at 352.482 kb on - strand, within MMJJ_RS01835 at 352.482 kb on - strand, within MMJJ_RS01835 at 352.483 kb on + strand, within MMJJ_RS01835 at 352.486 kb on - strand, within MMJJ_RS01835 at 352.486 kb on - strand, within MMJJ_RS01835 at 352.487 kb on + strand, within MMJJ_RS01835 at 352.489 kb on - strand, within MMJJ_RS01835 at 352.489 kb on - strand, within MMJJ_RS01835 at 352.490 kb on + strand, within MMJJ_RS01835 at 352.495 kb on + strand, within MMJJ_RS01835 at 352.539 kb on + strand, within MMJJ_RS01835 at 352.558 kb on + strand, within MMJJ_RS01835 at 352.558 kb on + strand, within MMJJ_RS01835 at 352.558 kb on + strand, within MMJJ_RS01835 at 352.584 kb on - strand, within MMJJ_RS01835 at 352.590 kb on - strand, within MMJJ_RS01835 at 352.590 kb on - strand, within MMJJ_RS01835 at 352.590 kb on - strand, within MMJJ_RS01835 at 352.591 kb on + strand, within MMJJ_RS01835 at 352.591 kb on + strand, within MMJJ_RS01835 at 352.606 kb on - strand, within MMJJ_RS01835 at 352.607 kb on + strand, within MMJJ_RS01835 at 352.686 kb on + strand, within MMJJ_RS01835 at 352.722 kb on - strand, within MMJJ_RS01835 at 352.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 349,480 + MMJJ_RS01820 0.45 -1.7 349,677 - -0.9 349,677 - -2.1 349,677 - +0.1 349,696 - +1.1 349,696 - +2.3 349,696 - +0.8 349,712 - +3.7 349,714 - +2.8 349,714 - -2.2 349,714 - +1.1 349,714 - -0.5 349,715 + +3.7 349,715 + -2.5 349,715 + +1.1 349,715 + -1.5 349,715 + -0.8 349,716 - -0.2 349,718 - -3.3 349,719 + +1.8 349,751 + -1.5 349,781 - +2.2 349,809 - -1.7 349,863 + MMJJ_RS01825 0.15 -4.2 349,863 + MMJJ_RS01825 0.15 -3.3 349,921 + MMJJ_RS01825 0.24 -3.1 349,954 + MMJJ_RS01825 0.29 -1.3 349,954 + MMJJ_RS01825 0.29 -1.8 349,976 - MMJJ_RS01825 0.32 -1.1 350,055 + MMJJ_RS01825 0.44 +0.1 350,093 + MMJJ_RS01825 0.50 -2.1 350,120 - MMJJ_RS01825 0.54 +0.8 350,120 - MMJJ_RS01825 0.54 +1.4 350,121 + MMJJ_RS01825 0.54 -0.1 350,121 + MMJJ_RS01825 0.54 -1.9 350,211 - MMJJ_RS01825 0.68 -2.7 350,334 + MMJJ_RS01825 0.87 +1.9 350,373 - -1.3 350,417 - +1.8 350,418 + -3.6 350,444 + -0.1 350,449 - +2.8 350,461 + -0.9 350,461 + -1.7 350,465 - -1.1 350,692 - MMJJ_RS01830 0.18 -0.9 350,803 + MMJJ_RS01830 0.27 -0.9 350,928 - MMJJ_RS01830 0.36 -0.9 351,034 - MMJJ_RS01830 0.45 -1.5 351,151 + MMJJ_RS01830 0.54 -0.9 351,248 - MMJJ_RS01830 0.61 -1.7 351,253 + MMJJ_RS01830 0.62 +0.1 351,400 - MMJJ_RS01830 0.73 +1.7 351,569 + MMJJ_RS01830 0.87 -1.9 351,675 - -1.7 351,686 - -2.5 351,708 - +1.1 351,734 - -3.0 351,750 + -2.0 351,808 - +0.5 351,808 - -1.8 351,809 + -3.9 351,809 + -1.5 351,812 - -0.2 351,813 + -2.3 351,813 + +1.4 351,813 + +0.1 351,813 + +1.8 351,813 + -1.5 351,817 + +0.3 351,835 + -1.8 351,837 + +1.0 351,839 + -2.5 351,839 + -0.3 351,839 + -0.5 351,839 + -0.7 351,863 - -0.5 351,881 - +1.3 351,916 - -1.7 351,916 - +1.7 352,016 + MMJJ_RS01835 0.12 +2.4 352,016 + MMJJ_RS01835 0.12 -0.5 352,016 + MMJJ_RS01835 0.12 -3.0 352,079 + MMJJ_RS01835 0.16 -1.4 352,099 - MMJJ_RS01835 0.18 -1.3 352,202 + MMJJ_RS01835 0.26 +1.8 352,213 - MMJJ_RS01835 0.27 +0.8 352,213 - MMJJ_RS01835 0.27 -0.9 352,214 + MMJJ_RS01835 0.27 +1.0 352,214 + MMJJ_RS01835 0.27 +1.3 352,214 + MMJJ_RS01835 0.27 +1.8 352,277 + MMJJ_RS01835 0.32 -1.5 352,280 - MMJJ_RS01835 0.32 -0.4 352,331 - MMJJ_RS01835 0.36 -2.4 352,344 + MMJJ_RS01835 0.37 +1.4 352,344 + MMJJ_RS01835 0.37 +0.7 352,432 - MMJJ_RS01835 0.44 -1.8 352,472 + MMJJ_RS01835 0.47 +0.5 352,482 - MMJJ_RS01835 0.48 -0.6 352,482 - MMJJ_RS01835 0.48 +0.9 352,482 - MMJJ_RS01835 0.48 -1.7 352,482 - MMJJ_RS01835 0.48 +3.2 352,483 + MMJJ_RS01835 0.48 +1.7 352,486 - MMJJ_RS01835 0.48 -1.9 352,486 - MMJJ_RS01835 0.48 -1.6 352,487 + MMJJ_RS01835 0.48 -0.4 352,489 - MMJJ_RS01835 0.48 -1.9 352,489 - MMJJ_RS01835 0.48 +0.5 352,490 + MMJJ_RS01835 0.48 +1.7 352,495 + MMJJ_RS01835 0.49 -3.0 352,539 + MMJJ_RS01835 0.52 +0.5 352,558 + MMJJ_RS01835 0.53 -1.5 352,558 + MMJJ_RS01835 0.53 +1.0 352,558 + MMJJ_RS01835 0.53 -3.0 352,584 - MMJJ_RS01835 0.55 -2.5 352,590 - MMJJ_RS01835 0.56 -3.1 352,590 - MMJJ_RS01835 0.56 +0.6 352,590 - MMJJ_RS01835 0.56 -4.0 352,591 + MMJJ_RS01835 0.56 +0.1 352,591 + MMJJ_RS01835 0.56 -1.0 352,606 - MMJJ_RS01835 0.57 +0.1 352,607 + MMJJ_RS01835 0.57 -2.9 352,686 + MMJJ_RS01835 0.63 +3.6 352,722 - MMJJ_RS01835 0.66 -3.1 352,723 + +1.1
Or see this region's nucleotide sequence