Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01620 and MMJJ_RS01625 are separated by 119 nucleotides MMJJ_RS01625 and MMJJ_RS01630 are separated by 21 nucleotides
MMJJ_RS01620: MMJJ_RS01620 - ketol-acid reductoisomerase, at 313,620 to 314,612
_RS01620
MMJJ_RS01625: MMJJ_RS01625 - bile acid:sodium symporter family protein, at 314,732 to 315,697
_RS01625
MMJJ_RS01630: MMJJ_RS01630 - hypothetical protein, at 315,719 to 316,171
_RS01630
Position (kb) 314
315
316 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 314.648 kb on - strand at 314.655 kb on + strand at 314.656 kb on - strand at 314.664 kb on - strand at 314.686 kb on + strand at 314.755 kb on + strand at 314.796 kb on + strand at 314.796 kb on + strand at 314.893 kb on - strand, within MMJJ_RS01625 at 314.911 kb on - strand, within MMJJ_RS01625 at 314.920 kb on + strand, within MMJJ_RS01625 at 314.923 kb on - strand, within MMJJ_RS01625 at 314.923 kb on - strand, within MMJJ_RS01625 at 314.946 kb on - strand, within MMJJ_RS01625 at 314.989 kb on - strand, within MMJJ_RS01625 at 315.016 kb on + strand, within MMJJ_RS01625 at 315.022 kb on - strand, within MMJJ_RS01625 at 315.023 kb on + strand, within MMJJ_RS01625 at 315.027 kb on - strand, within MMJJ_RS01625 at 315.036 kb on - strand, within MMJJ_RS01625 at 315.036 kb on - strand, within MMJJ_RS01625 at 315.037 kb on + strand, within MMJJ_RS01625 at 315.066 kb on - strand, within MMJJ_RS01625 at 315.102 kb on - strand, within MMJJ_RS01625 at 315.102 kb on - strand, within MMJJ_RS01625 at 315.103 kb on + strand, within MMJJ_RS01625 at 315.103 kb on + strand, within MMJJ_RS01625 at 315.105 kb on - strand, within MMJJ_RS01625 at 315.106 kb on + strand, within MMJJ_RS01625 at 315.106 kb on + strand, within MMJJ_RS01625 at 315.106 kb on + strand, within MMJJ_RS01625 at 315.106 kb on + strand, within MMJJ_RS01625 at 315.106 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.111 kb on + strand, within MMJJ_RS01625 at 315.111 kb on + strand, within MMJJ_RS01625 at 315.111 kb on + strand, within MMJJ_RS01625 at 315.182 kb on + strand, within MMJJ_RS01625 at 315.195 kb on - strand, within MMJJ_RS01625 at 315.195 kb on - strand, within MMJJ_RS01625 at 315.198 kb on - strand, within MMJJ_RS01625 at 315.199 kb on + strand, within MMJJ_RS01625 at 315.199 kb on + strand, within MMJJ_RS01625 at 315.219 kb on - strand, within MMJJ_RS01625 at 315.234 kb on + strand, within MMJJ_RS01625 at 315.247 kb on + strand, within MMJJ_RS01625 at 315.264 kb on - strand, within MMJJ_RS01625 at 315.282 kb on - strand, within MMJJ_RS01625 at 315.289 kb on + strand, within MMJJ_RS01625 at 315.293 kb on + strand, within MMJJ_RS01625 at 315.294 kb on - strand, within MMJJ_RS01625 at 315.294 kb on - strand, within MMJJ_RS01625 at 315.294 kb on - strand, within MMJJ_RS01625 at 315.298 kb on - strand, within MMJJ_RS01625 at 315.298 kb on - strand, within MMJJ_RS01625 at 315.340 kb on + strand, within MMJJ_RS01625 at 315.364 kb on + strand, within MMJJ_RS01625 at 315.411 kb on - strand, within MMJJ_RS01625 at 315.412 kb on + strand, within MMJJ_RS01625 at 315.424 kb on - strand, within MMJJ_RS01625 at 315.425 kb on + strand, within MMJJ_RS01625 at 315.432 kb on - strand, within MMJJ_RS01625 at 315.478 kb on - strand, within MMJJ_RS01625 at 315.478 kb on - strand, within MMJJ_RS01625 at 315.478 kb on - strand, within MMJJ_RS01625 at 315.526 kb on - strand, within MMJJ_RS01625 at 315.531 kb on - strand, within MMJJ_RS01625 at 315.531 kb on - strand, within MMJJ_RS01625 at 315.531 kb on - strand, within MMJJ_RS01625 at 315.532 kb on + strand, within MMJJ_RS01625 at 315.564 kb on - strand, within MMJJ_RS01625 at 315.565 kb on + strand, within MMJJ_RS01625 at 315.637 kb on - strand at 315.638 kb on + strand at 315.659 kb on - strand at 315.710 kb on + strand at 315.712 kb on + strand at 315.712 kb on + strand at 315.720 kb on - strand at 315.721 kb on + strand at 315.721 kb on + strand at 315.722 kb on - strand at 315.722 kb on - strand at 315.723 kb on + strand at 315.732 kb on + strand at 315.768 kb on + strand, within MMJJ_RS01630 at 315.768 kb on + strand, within MMJJ_RS01630 at 315.771 kb on - strand, within MMJJ_RS01630 at 315.771 kb on - strand, within MMJJ_RS01630 at 315.788 kb on + strand, within MMJJ_RS01630 at 315.815 kb on - strand, within MMJJ_RS01630 at 315.815 kb on - strand, within MMJJ_RS01630 at 315.831 kb on + strand, within MMJJ_RS01630 at 315.861 kb on + strand, within MMJJ_RS01630 at 315.897 kb on + strand, within MMJJ_RS01630 at 315.927 kb on + strand, within MMJJ_RS01630 at 315.937 kb on - strand, within MMJJ_RS01630 at 315.938 kb on + strand at 315.963 kb on + strand, within MMJJ_RS01630 at 315.963 kb on + strand, within MMJJ_RS01630 at 315.987 kb on - strand, within MMJJ_RS01630 at 315.988 kb on + strand, within MMJJ_RS01630 at 315.988 kb on + strand, within MMJJ_RS01630 at 315.988 kb on + strand, within MMJJ_RS01630 at 315.996 kb on + strand, within MMJJ_RS01630 at 315.996 kb on + strand, within MMJJ_RS01630 at 316.008 kb on - strand, within MMJJ_RS01630 at 316.016 kb on - strand, within MMJJ_RS01630 at 316.016 kb on - strand, within MMJJ_RS01630 at 316.022 kb on + strand, within MMJJ_RS01630 at 316.063 kb on + strand, within MMJJ_RS01630 at 316.063 kb on + strand, within MMJJ_RS01630 at 316.064 kb on - strand, within MMJJ_RS01630 at 316.072 kb on - strand, within MMJJ_RS01630 at 316.072 kb on - strand, within MMJJ_RS01630 at 316.098 kb on + strand, within MMJJ_RS01630 at 316.098 kb on + strand, within MMJJ_RS01630 at 316.103 kb on + strand, within MMJJ_RS01630 at 316.249 kb on - strand at 316.249 kb on - strand at 316.250 kb on + strand at 316.252 kb on + strand at 316.270 kb on - strand at 316.271 kb on + strand at 316.271 kb on + strand at 316.271 kb on + strand at 316.272 kb on - strand at 316.273 kb on + strand at 316.361 kb on + strand at 316.374 kb on - strand at 316.375 kb on + strand at 316.378 kb on + strand at 316.397 kb on + strand at 316.397 kb on + strand at 316.397 kb on + strand at 316.408 kb on - strand at 316.409 kb on + strand at 316.410 kb on - strand at 316.439 kb on - strand at 316.472 kb on - strand at 316.555 kb on - strand at 316.573 kb on - strand at 316.676 kb on + strand at 316.679 kb on - strand at 316.680 kb on + strand at 316.680 kb on + strand at 316.680 kb on + strand at 316.680 kb on + strand at 316.682 kb on - strand at 316.682 kb on - strand at 316.682 kb on - strand at 316.682 kb on - strand at 316.683 kb on + strand at 316.683 kb on + strand at 316.683 kb on + strand at 316.685 kb on + strand at 316.685 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 314,648 - -0.5 314,655 + -1.5 314,656 - +1.1 314,664 - +1.4 314,686 + +4.7 314,755 + -1.3 314,796 + +0.4 314,796 + -0.1 314,893 - MMJJ_RS01625 0.17 -1.2 314,911 - MMJJ_RS01625 0.19 +1.8 314,920 + MMJJ_RS01625 0.19 +0.6 314,923 - MMJJ_RS01625 0.20 +0.1 314,923 - MMJJ_RS01625 0.20 +1.0 314,946 - MMJJ_RS01625 0.22 -3.2 314,989 - MMJJ_RS01625 0.27 -1.9 315,016 + MMJJ_RS01625 0.29 -0.5 315,022 - MMJJ_RS01625 0.30 +1.7 315,023 + MMJJ_RS01625 0.30 -0.5 315,027 - MMJJ_RS01625 0.31 +0.0 315,036 - MMJJ_RS01625 0.31 +0.5 315,036 - MMJJ_RS01625 0.31 +3.9 315,037 + MMJJ_RS01625 0.32 +2.6 315,066 - MMJJ_RS01625 0.35 +3.2 315,102 - MMJJ_RS01625 0.38 +2.1 315,102 - MMJJ_RS01625 0.38 +2.3 315,103 + MMJJ_RS01625 0.38 -1.6 315,103 + MMJJ_RS01625 0.38 +1.7 315,105 - MMJJ_RS01625 0.39 -1.3 315,106 + MMJJ_RS01625 0.39 -2.8 315,106 + MMJJ_RS01625 0.39 -2.1 315,106 + MMJJ_RS01625 0.39 -1.4 315,106 + MMJJ_RS01625 0.39 -0.5 315,106 - MMJJ_RS01625 0.39 +2.0 315,110 - MMJJ_RS01625 0.39 +3.4 315,110 - MMJJ_RS01625 0.39 +0.7 315,110 - MMJJ_RS01625 0.39 +3.0 315,110 - MMJJ_RS01625 0.39 -4.1 315,110 - MMJJ_RS01625 0.39 +0.3 315,110 - MMJJ_RS01625 0.39 +1.7 315,111 + MMJJ_RS01625 0.39 -1.4 315,111 + MMJJ_RS01625 0.39 -2.5 315,111 + MMJJ_RS01625 0.39 -0.1 315,182 + MMJJ_RS01625 0.47 +2.2 315,195 - MMJJ_RS01625 0.48 +0.8 315,195 - MMJJ_RS01625 0.48 +1.9 315,198 - MMJJ_RS01625 0.48 +3.1 315,199 + MMJJ_RS01625 0.48 +1.1 315,199 + MMJJ_RS01625 0.48 -1.5 315,219 - MMJJ_RS01625 0.50 -2.9 315,234 + MMJJ_RS01625 0.52 +1.2 315,247 + MMJJ_RS01625 0.53 -2.2 315,264 - MMJJ_RS01625 0.55 -0.2 315,282 - MMJJ_RS01625 0.57 +1.5 315,289 + MMJJ_RS01625 0.58 +3.3 315,293 + MMJJ_RS01625 0.58 -1.5 315,294 - MMJJ_RS01625 0.58 -2.4 315,294 - MMJJ_RS01625 0.58 +3.8 315,294 - MMJJ_RS01625 0.58 -0.9 315,298 - MMJJ_RS01625 0.59 -2.4 315,298 - MMJJ_RS01625 0.59 +1.2 315,340 + MMJJ_RS01625 0.63 -2.5 315,364 + MMJJ_RS01625 0.65 +1.7 315,411 - MMJJ_RS01625 0.70 +0.9 315,412 + MMJJ_RS01625 0.70 -0.2 315,424 - MMJJ_RS01625 0.72 +0.8 315,425 + MMJJ_RS01625 0.72 +1.1 315,432 - MMJJ_RS01625 0.72 +0.8 315,478 - MMJJ_RS01625 0.77 -1.2 315,478 - MMJJ_RS01625 0.77 +0.7 315,478 - MMJJ_RS01625 0.77 -2.4 315,526 - MMJJ_RS01625 0.82 +3.3 315,531 - MMJJ_RS01625 0.83 +2.4 315,531 - MMJJ_RS01625 0.83 -0.9 315,531 - MMJJ_RS01625 0.83 -1.1 315,532 + MMJJ_RS01625 0.83 +0.2 315,564 - MMJJ_RS01625 0.86 -3.4 315,565 + MMJJ_RS01625 0.86 -1.2 315,637 - +1.9 315,638 + -1.3 315,659 - +3.4 315,710 + -0.0 315,712 + +2.9 315,712 + +1.2 315,720 - -0.7 315,721 + +0.1 315,721 + +2.7 315,722 - +1.2 315,722 - -2.5 315,723 + -0.7 315,732 + +1.7 315,768 + MMJJ_RS01630 0.11 +2.0 315,768 + MMJJ_RS01630 0.11 +0.5 315,771 - MMJJ_RS01630 0.11 +1.9 315,771 - MMJJ_RS01630 0.11 -1.2 315,788 + MMJJ_RS01630 0.15 +3.2 315,815 - MMJJ_RS01630 0.21 +1.7 315,815 - MMJJ_RS01630 0.21 -1.2 315,831 + MMJJ_RS01630 0.25 +0.5 315,861 + MMJJ_RS01630 0.31 -1.7 315,897 + MMJJ_RS01630 0.39 -0.2 315,927 + MMJJ_RS01630 0.46 +3.3 315,937 - MMJJ_RS01630 0.48 -0.8 315,938 + +0.1 315,963 + MMJJ_RS01630 0.54 -0.0 315,963 + MMJJ_RS01630 0.54 +2.6 315,987 - MMJJ_RS01630 0.59 +0.2 315,988 + MMJJ_RS01630 0.59 +0.3 315,988 + MMJJ_RS01630 0.59 -2.1 315,988 + MMJJ_RS01630 0.59 +0.5 315,996 + MMJJ_RS01630 0.61 +0.4 315,996 + MMJJ_RS01630 0.61 -3.1 316,008 - MMJJ_RS01630 0.64 +1.5 316,016 - MMJJ_RS01630 0.66 +1.4 316,016 - MMJJ_RS01630 0.66 +0.4 316,022 + MMJJ_RS01630 0.67 +1.1 316,063 + MMJJ_RS01630 0.76 +1.2 316,063 + MMJJ_RS01630 0.76 -2.4 316,064 - MMJJ_RS01630 0.76 -3.0 316,072 - MMJJ_RS01630 0.78 -1.2 316,072 - MMJJ_RS01630 0.78 -2.0 316,098 + MMJJ_RS01630 0.84 +1.0 316,098 + MMJJ_RS01630 0.84 -1.7 316,103 + MMJJ_RS01630 0.85 +3.0 316,249 - +3.5 316,249 - +1.6 316,250 + -2.0 316,252 + -1.2 316,270 - +0.8 316,271 + -0.9 316,271 + +2.1 316,271 + -3.4 316,272 - -1.9 316,273 + -0.9 316,361 + +0.1 316,374 - -0.5 316,375 + +4.4 316,378 + -1.9 316,397 + +1.5 316,397 + +1.1 316,397 + -1.9 316,408 - -0.8 316,409 + -0.9 316,410 - -0.9 316,439 - -0.5 316,472 - -1.1 316,555 - +3.8 316,573 - +1.6 316,676 + -0.7 316,679 - +2.0 316,680 + -1.5 316,680 + +1.6 316,680 + +4.1 316,680 + +2.6 316,682 - -2.9 316,682 - -0.4 316,682 - +3.0 316,682 - -1.5 316,683 + -0.1 316,683 + +0.3 316,683 + +1.6 316,685 + +5.3 316,685 + +1.3
Or see this region's nucleotide sequence