Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01525 and MMJJ_RS01530 are separated by 102 nucleotides MMJJ_RS01530 and MMJJ_RS01535 are separated by 9 nucleotides MMJJ_RS01535 and MMJJ_RS01540 are separated by 85 nucleotides
MMJJ_RS01525: MMJJ_RS01525 - ketopantoate reductase family protein, at 297,845 to 298,765
_RS01525
MMJJ_RS01530: MMJJ_RS01530 - YwbE family protein, at 298,868 to 299,062
_RS01530
MMJJ_RS01535: MMJJ_RS01535 - DMT family transporter, at 299,072 to 299,962
_RS01535
MMJJ_RS01540: MMJJ_RS01540 - 30S ribosomal protein S3ae, at 300,048 to 300,737
_RS01540
Position (kb) 298
299
300 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 297.901 kb on - strand at 297.906 kb on - strand at 297.965 kb on - strand, within MMJJ_RS01525 at 297.965 kb on - strand, within MMJJ_RS01525 at 297.976 kb on - strand, within MMJJ_RS01525 at 297.976 kb on - strand, within MMJJ_RS01525 at 297.977 kb on + strand, within MMJJ_RS01525 at 298.059 kb on - strand, within MMJJ_RS01525 at 298.124 kb on - strand, within MMJJ_RS01525 at 298.129 kb on - strand, within MMJJ_RS01525 at 298.130 kb on + strand, within MMJJ_RS01525 at 298.130 kb on + strand, within MMJJ_RS01525 at 298.135 kb on - strand, within MMJJ_RS01525 at 298.141 kb on - strand, within MMJJ_RS01525 at 298.142 kb on + strand, within MMJJ_RS01525 at 298.142 kb on + strand, within MMJJ_RS01525 at 298.186 kb on - strand, within MMJJ_RS01525 at 298.217 kb on + strand, within MMJJ_RS01525 at 298.218 kb on - strand, within MMJJ_RS01525 at 298.250 kb on + strand, within MMJJ_RS01525 at 298.361 kb on - strand, within MMJJ_RS01525 at 298.362 kb on + strand, within MMJJ_RS01525 at 298.408 kb on - strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.430 kb on + strand, within MMJJ_RS01525 at 298.435 kb on - strand, within MMJJ_RS01525 at 298.435 kb on - strand, within MMJJ_RS01525 at 298.436 kb on + strand, within MMJJ_RS01525 at 298.436 kb on + strand, within MMJJ_RS01525 at 298.437 kb on - strand, within MMJJ_RS01525 at 298.443 kb on + strand, within MMJJ_RS01525 at 298.500 kb on + strand, within MMJJ_RS01525 at 298.500 kb on + strand, within MMJJ_RS01525 at 298.501 kb on - strand at 298.501 kb on - strand, within MMJJ_RS01525 at 298.553 kb on - strand, within MMJJ_RS01525 at 298.554 kb on + strand, within MMJJ_RS01525 at 298.555 kb on - strand, within MMJJ_RS01525 at 298.555 kb on - strand, within MMJJ_RS01525 at 298.556 kb on + strand, within MMJJ_RS01525 at 298.558 kb on - strand, within MMJJ_RS01525 at 298.558 kb on - strand, within MMJJ_RS01525 at 298.559 kb on + strand, within MMJJ_RS01525 at 298.561 kb on - strand, within MMJJ_RS01525 at 298.563 kb on - strand, within MMJJ_RS01525 at 298.563 kb on - strand, within MMJJ_RS01525 at 298.656 kb on + strand, within MMJJ_RS01525 at 298.700 kb on - strand at 298.721 kb on - strand at 298.727 kb on - strand at 298.754 kb on - strand at 298.820 kb on - strand at 298.820 kb on - strand at 298.821 kb on + strand at 298.823 kb on - strand at 298.826 kb on - strand at 298.827 kb on + strand at 298.835 kb on - strand at 298.836 kb on + strand at 298.839 kb on - strand at 298.840 kb on + strand at 298.843 kb on - strand at 298.843 kb on - strand at 298.844 kb on + strand at 298.844 kb on + strand at 298.870 kb on + strand at 298.922 kb on - strand, within MMJJ_RS01530 at 298.923 kb on + strand, within MMJJ_RS01530 at 299.012 kb on - strand, within MMJJ_RS01530 at 299.037 kb on + strand, within MMJJ_RS01530 at 299.063 kb on + strand at 299.065 kb on - strand at 299.152 kb on + strand at 299.155 kb on - strand at 299.164 kb on - strand, within MMJJ_RS01535 at 299.178 kb on + strand, within MMJJ_RS01535 at 299.212 kb on - strand, within MMJJ_RS01535 at 299.212 kb on - strand, within MMJJ_RS01535 at 299.213 kb on + strand, within MMJJ_RS01535 at 299.214 kb on - strand, within MMJJ_RS01535 at 299.234 kb on + strand, within MMJJ_RS01535 at 299.260 kb on - strand, within MMJJ_RS01535 at 299.274 kb on - strand, within MMJJ_RS01535 at 299.274 kb on - strand, within MMJJ_RS01535 at 299.279 kb on - strand, within MMJJ_RS01535 at 299.280 kb on + strand, within MMJJ_RS01535 at 299.304 kb on - strand, within MMJJ_RS01535 at 299.304 kb on - strand, within MMJJ_RS01535 at 299.340 kb on + strand, within MMJJ_RS01535 at 299.344 kb on - strand, within MMJJ_RS01535 at 299.344 kb on - strand, within MMJJ_RS01535 at 299.345 kb on + strand, within MMJJ_RS01535 at 299.345 kb on + strand, within MMJJ_RS01535 at 299.376 kb on + strand, within MMJJ_RS01535 at 299.383 kb on - strand, within MMJJ_RS01535 at 299.384 kb on + strand, within MMJJ_RS01535 at 299.392 kb on - strand, within MMJJ_RS01535 at 299.392 kb on - strand, within MMJJ_RS01535 at 299.399 kb on - strand, within MMJJ_RS01535 at 299.417 kb on - strand, within MMJJ_RS01535 at 299.418 kb on + strand, within MMJJ_RS01535 at 299.475 kb on - strand, within MMJJ_RS01535 at 299.476 kb on + strand, within MMJJ_RS01535 at 299.488 kb on - strand, within MMJJ_RS01535 at 299.489 kb on + strand, within MMJJ_RS01535 at 299.510 kb on - strand, within MMJJ_RS01535 at 299.511 kb on + strand, within MMJJ_RS01535 at 299.513 kb on + strand, within MMJJ_RS01535 at 299.513 kb on + strand, within MMJJ_RS01535 at 299.513 kb on + strand, within MMJJ_RS01535 at 299.603 kb on - strand, within MMJJ_RS01535 at 299.655 kb on + strand, within MMJJ_RS01535 at 299.655 kb on + strand, within MMJJ_RS01535 at 299.656 kb on - strand, within MMJJ_RS01535 at 299.656 kb on - strand, within MMJJ_RS01535 at 299.661 kb on + strand, within MMJJ_RS01535 at 299.676 kb on + strand, within MMJJ_RS01535 at 299.687 kb on + strand, within MMJJ_RS01535 at 299.687 kb on + strand, within MMJJ_RS01535 at 299.700 kb on - strand, within MMJJ_RS01535 at 299.701 kb on + strand, within MMJJ_RS01535 at 299.706 kb on + strand, within MMJJ_RS01535 at 299.706 kb on + strand, within MMJJ_RS01535 at 299.723 kb on - strand, within MMJJ_RS01535 at 299.727 kb on - strand, within MMJJ_RS01535 at 299.728 kb on + strand, within MMJJ_RS01535 at 299.731 kb on + strand, within MMJJ_RS01535 at 299.737 kb on - strand, within MMJJ_RS01535 at 299.737 kb on - strand, within MMJJ_RS01535 at 299.766 kb on - strand, within MMJJ_RS01535 at 299.767 kb on + strand, within MMJJ_RS01535 at 299.767 kb on + strand, within MMJJ_RS01535 at 299.834 kb on - strand, within MMJJ_RS01535 at 299.835 kb on + strand, within MMJJ_RS01535 at 299.835 kb on + strand, within MMJJ_RS01535 at 299.835 kb on + strand, within MMJJ_RS01535 at 299.835 kb on + strand, within MMJJ_RS01535 at 299.844 kb on + strand, within MMJJ_RS01535 at 299.845 kb on - strand, within MMJJ_RS01535 at 299.853 kb on + strand, within MMJJ_RS01535 at 299.853 kb on + strand, within MMJJ_RS01535 at 299.857 kb on - strand, within MMJJ_RS01535 at 299.857 kb on - strand, within MMJJ_RS01535 at 299.858 kb on + strand, within MMJJ_RS01535 at 299.858 kb on + strand, within MMJJ_RS01535 at 299.858 kb on + strand, within MMJJ_RS01535 at 299.876 kb on - strand at 299.877 kb on + strand at 299.895 kb on + strand at 299.925 kb on + strand at 299.985 kb on - strand at 299.989 kb on - strand at 300.003 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 297,901 - -0.0 297,906 - +3.5 297,965 - MMJJ_RS01525 0.13 -2.3 297,965 - MMJJ_RS01525 0.13 +1.4 297,976 - MMJJ_RS01525 0.14 -1.8 297,976 - MMJJ_RS01525 0.14 -0.7 297,977 + MMJJ_RS01525 0.14 +1.1 298,059 - MMJJ_RS01525 0.23 -2.3 298,124 - MMJJ_RS01525 0.30 +3.1 298,129 - MMJJ_RS01525 0.31 -1.7 298,130 + MMJJ_RS01525 0.31 -2.4 298,130 + MMJJ_RS01525 0.31 +2.2 298,135 - MMJJ_RS01525 0.31 -1.5 298,141 - MMJJ_RS01525 0.32 -4.0 298,142 + MMJJ_RS01525 0.32 +0.1 298,142 + MMJJ_RS01525 0.32 +2.4 298,186 - MMJJ_RS01525 0.37 -3.3 298,217 + MMJJ_RS01525 0.40 +3.5 298,218 - MMJJ_RS01525 0.40 +0.7 298,250 + MMJJ_RS01525 0.44 +4.0 298,361 - MMJJ_RS01525 0.56 +2.4 298,362 + MMJJ_RS01525 0.56 +4.5 298,408 - MMJJ_RS01525 0.61 +2.2 298,412 + MMJJ_RS01525 0.62 +2.2 298,412 + MMJJ_RS01525 0.62 +1.7 298,412 + MMJJ_RS01525 0.62 +0.6 298,430 + MMJJ_RS01525 0.64 +0.3 298,435 - MMJJ_RS01525 0.64 -1.5 298,435 - MMJJ_RS01525 0.64 +0.5 298,436 + MMJJ_RS01525 0.64 -1.3 298,436 + MMJJ_RS01525 0.64 -0.7 298,437 - MMJJ_RS01525 0.64 -0.5 298,443 + MMJJ_RS01525 0.65 +3.3 298,500 + MMJJ_RS01525 0.71 +1.4 298,500 + MMJJ_RS01525 0.71 -1.2 298,501 - +0.1 298,501 - MMJJ_RS01525 0.71 +2.1 298,553 - MMJJ_RS01525 0.77 +0.6 298,554 + MMJJ_RS01525 0.77 +0.1 298,555 - MMJJ_RS01525 0.77 +5.0 298,555 - MMJJ_RS01525 0.77 +2.1 298,556 + MMJJ_RS01525 0.77 +1.0 298,558 - MMJJ_RS01525 0.77 +1.2 298,558 - MMJJ_RS01525 0.77 +1.3 298,559 + MMJJ_RS01525 0.78 +2.4 298,561 - MMJJ_RS01525 0.78 +1.8 298,563 - MMJJ_RS01525 0.78 +3.7 298,563 - MMJJ_RS01525 0.78 +1.7 298,656 + MMJJ_RS01525 0.88 +0.1 298,700 - -1.5 298,721 - +1.0 298,727 - +3.7 298,754 - +2.0 298,820 - -2.1 298,820 - +3.6 298,821 + +2.1 298,823 - -3.9 298,826 - -2.8 298,827 + +0.4 298,835 - -1.6 298,836 + -0.9 298,839 - +0.3 298,840 + +2.8 298,843 - +2.5 298,843 - +1.1 298,844 + +0.7 298,844 + -1.9 298,870 + +0.8 298,922 - MMJJ_RS01530 0.28 -2.2 298,923 + MMJJ_RS01530 0.28 -1.5 299,012 - MMJJ_RS01530 0.74 -1.1 299,037 + MMJJ_RS01530 0.87 -2.5 299,063 + +7.7 299,065 - -0.1 299,152 + -0.5 299,155 - +3.9 299,164 - MMJJ_RS01535 0.10 -0.9 299,178 + MMJJ_RS01535 0.12 +4.0 299,212 - MMJJ_RS01535 0.16 -1.7 299,212 - MMJJ_RS01535 0.16 -1.7 299,213 + MMJJ_RS01535 0.16 -4.0 299,214 - MMJJ_RS01535 0.16 -0.9 299,234 + MMJJ_RS01535 0.18 +0.7 299,260 - MMJJ_RS01535 0.21 +0.6 299,274 - MMJJ_RS01535 0.23 +1.6 299,274 - MMJJ_RS01535 0.23 -0.1 299,279 - MMJJ_RS01535 0.23 -0.0 299,280 + MMJJ_RS01535 0.23 -0.5 299,304 - MMJJ_RS01535 0.26 -1.4 299,304 - MMJJ_RS01535 0.26 +1.2 299,340 + MMJJ_RS01535 0.30 -0.4 299,344 - MMJJ_RS01535 0.31 +0.7 299,344 - MMJJ_RS01535 0.31 -2.1 299,345 + MMJJ_RS01535 0.31 +0.6 299,345 + MMJJ_RS01535 0.31 +2.8 299,376 + MMJJ_RS01535 0.34 -0.4 299,383 - MMJJ_RS01535 0.35 -0.3 299,384 + MMJJ_RS01535 0.35 +2.2 299,392 - MMJJ_RS01535 0.36 +2.0 299,392 - MMJJ_RS01535 0.36 -3.7 299,399 - MMJJ_RS01535 0.37 -1.5 299,417 - MMJJ_RS01535 0.39 -2.7 299,418 + MMJJ_RS01535 0.39 -1.7 299,475 - MMJJ_RS01535 0.45 +1.4 299,476 + MMJJ_RS01535 0.45 -2.6 299,488 - MMJJ_RS01535 0.47 -2.2 299,489 + MMJJ_RS01535 0.47 -1.5 299,510 - MMJJ_RS01535 0.49 -2.9 299,511 + MMJJ_RS01535 0.49 +0.8 299,513 + MMJJ_RS01535 0.49 +2.1 299,513 + MMJJ_RS01535 0.49 +0.7 299,513 + MMJJ_RS01535 0.49 -2.8 299,603 - MMJJ_RS01535 0.60 +1.5 299,655 + MMJJ_RS01535 0.65 -2.1 299,655 + MMJJ_RS01535 0.65 +0.4 299,656 - MMJJ_RS01535 0.66 -1.8 299,656 - MMJJ_RS01535 0.66 +0.6 299,661 + MMJJ_RS01535 0.66 -0.7 299,676 + MMJJ_RS01535 0.68 +1.5 299,687 + MMJJ_RS01535 0.69 -1.2 299,687 + MMJJ_RS01535 0.69 +0.1 299,700 - MMJJ_RS01535 0.70 -1.2 299,701 + MMJJ_RS01535 0.71 -1.5 299,706 + MMJJ_RS01535 0.71 -0.6 299,706 + MMJJ_RS01535 0.71 -0.9 299,723 - MMJJ_RS01535 0.73 +0.7 299,727 - MMJJ_RS01535 0.74 +2.1 299,728 + MMJJ_RS01535 0.74 -1.2 299,731 + MMJJ_RS01535 0.74 +2.0 299,737 - MMJJ_RS01535 0.75 +1.2 299,737 - MMJJ_RS01535 0.75 -0.3 299,766 - MMJJ_RS01535 0.78 -0.2 299,767 + MMJJ_RS01535 0.78 -2.5 299,767 + MMJJ_RS01535 0.78 -2.1 299,834 - MMJJ_RS01535 0.86 +1.4 299,835 + MMJJ_RS01535 0.86 -0.2 299,835 + MMJJ_RS01535 0.86 -0.1 299,835 + MMJJ_RS01535 0.86 -0.9 299,835 + MMJJ_RS01535 0.86 -2.6 299,844 + MMJJ_RS01535 0.87 -0.1 299,845 - MMJJ_RS01535 0.87 -1.7 299,853 + MMJJ_RS01535 0.88 +0.1 299,853 + MMJJ_RS01535 0.88 -1.9 299,857 - MMJJ_RS01535 0.88 +0.8 299,857 - MMJJ_RS01535 0.88 -0.2 299,858 + MMJJ_RS01535 0.88 +0.8 299,858 + MMJJ_RS01535 0.88 -1.2 299,858 + MMJJ_RS01535 0.88 +1.7 299,876 - -2.2 299,877 + +0.1 299,895 + +0.7 299,925 + -0.8 299,985 - +1.0 299,989 - -1.7 300,003 + +0.5
Or see this region's nucleotide sequence