Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01510 and MMJJ_RS01515 are separated by 130 nucleotides MMJJ_RS01515 and MMJJ_RS01520 are separated by 10 nucleotides MMJJ_RS01520 and MMJJ_RS01525 are separated by 14 nucleotides
MMJJ_RS01510: MMJJ_RS01510 - ATP-binding protein, at 295,702 to 297,099
_RS01510
MMJJ_RS01515: MMJJ_RS01515 - DUF2178 domain-containing protein, at 297,230 to 297,616
_RS01515
MMJJ_RS01520: MMJJ_RS01520 - helix-turn-helix transcriptional regulator, at 297,627 to 297,830
_RS01520
MMJJ_RS01525: MMJJ_RS01525 - ketopantoate reductase family protein, at 297,845 to 298,765
_RS01525
Position (kb)
297
298 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 296.248 kb on + strand, within MMJJ_RS01510 at 296.265 kb on - strand at 296.265 kb on - strand, within MMJJ_RS01510 at 296.304 kb on - strand, within MMJJ_RS01510 at 296.337 kb on - strand, within MMJJ_RS01510 at 296.378 kb on + strand, within MMJJ_RS01510 at 296.384 kb on + strand, within MMJJ_RS01510 at 296.409 kb on - strand, within MMJJ_RS01510 at 296.443 kb on + strand, within MMJJ_RS01510 at 296.451 kb on + strand, within MMJJ_RS01510 at 296.463 kb on - strand, within MMJJ_RS01510 at 296.501 kb on - strand, within MMJJ_RS01510 at 296.501 kb on - strand, within MMJJ_RS01510 at 296.586 kb on + strand, within MMJJ_RS01510 at 296.653 kb on - strand, within MMJJ_RS01510 at 296.712 kb on + strand, within MMJJ_RS01510 at 296.744 kb on - strand, within MMJJ_RS01510 at 296.874 kb on - strand, within MMJJ_RS01510 at 296.916 kb on - strand, within MMJJ_RS01510 at 297.015 kb on + strand at 297.066 kb on - strand at 297.069 kb on + strand at 297.073 kb on - strand at 297.074 kb on + strand at 297.076 kb on + strand at 297.088 kb on - strand at 297.107 kb on + strand at 297.115 kb on - strand at 297.120 kb on - strand at 297.121 kb on + strand at 297.126 kb on + strand at 297.126 kb on + strand at 297.126 kb on + strand at 297.129 kb on + strand at 297.195 kb on + strand at 297.208 kb on - strand at 297.254 kb on - strand at 297.259 kb on - strand at 297.273 kb on - strand, within MMJJ_RS01515 at 297.274 kb on + strand, within MMJJ_RS01515 at 297.321 kb on + strand, within MMJJ_RS01515 at 297.343 kb on + strand, within MMJJ_RS01515 at 297.370 kb on - strand, within MMJJ_RS01515 at 297.371 kb on + strand, within MMJJ_RS01515 at 297.394 kb on + strand, within MMJJ_RS01515 at 297.394 kb on + strand, within MMJJ_RS01515 at 297.504 kb on + strand, within MMJJ_RS01515 at 297.504 kb on + strand, within MMJJ_RS01515 at 297.552 kb on - strand, within MMJJ_RS01515 at 297.567 kb on - strand, within MMJJ_RS01515 at 297.567 kb on - strand, within MMJJ_RS01515 at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.588 kb on - strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.589 kb on + strand at 297.641 kb on - strand at 297.651 kb on + strand, within MMJJ_RS01520 at 297.717 kb on + strand, within MMJJ_RS01520 at 297.757 kb on - strand, within MMJJ_RS01520 at 297.757 kb on - strand, within MMJJ_RS01520 at 297.779 kb on + strand, within MMJJ_RS01520 at 297.802 kb on - strand, within MMJJ_RS01520 at 297.802 kb on - strand, within MMJJ_RS01520 at 297.807 kb on + strand, within MMJJ_RS01520 at 297.842 kb on - strand at 297.864 kb on + strand at 297.901 kb on - strand at 297.906 kb on - strand at 297.965 kb on - strand, within MMJJ_RS01525 at 297.965 kb on - strand, within MMJJ_RS01525 at 297.976 kb on - strand, within MMJJ_RS01525 at 297.976 kb on - strand, within MMJJ_RS01525 at 297.977 kb on + strand, within MMJJ_RS01525 at 298.059 kb on - strand, within MMJJ_RS01525 at 298.124 kb on - strand, within MMJJ_RS01525 at 298.129 kb on - strand, within MMJJ_RS01525 at 298.130 kb on + strand, within MMJJ_RS01525 at 298.130 kb on + strand, within MMJJ_RS01525 at 298.135 kb on - strand, within MMJJ_RS01525 at 298.141 kb on - strand, within MMJJ_RS01525 at 298.142 kb on + strand, within MMJJ_RS01525 at 298.142 kb on + strand, within MMJJ_RS01525 at 298.186 kb on - strand, within MMJJ_RS01525 at 298.217 kb on + strand, within MMJJ_RS01525 at 298.218 kb on - strand, within MMJJ_RS01525 at 298.250 kb on + strand, within MMJJ_RS01525 at 298.361 kb on - strand, within MMJJ_RS01525 at 298.362 kb on + strand, within MMJJ_RS01525 at 298.408 kb on - strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.412 kb on + strand, within MMJJ_RS01525 at 298.430 kb on + strand, within MMJJ_RS01525 at 298.435 kb on - strand, within MMJJ_RS01525 at 298.435 kb on - strand, within MMJJ_RS01525 at 298.436 kb on + strand, within MMJJ_RS01525 at 298.436 kb on + strand, within MMJJ_RS01525 at 298.437 kb on - strand, within MMJJ_RS01525 at 298.443 kb on + strand, within MMJJ_RS01525 at 298.500 kb on + strand, within MMJJ_RS01525 at 298.500 kb on + strand, within MMJJ_RS01525 at 298.501 kb on - strand at 298.501 kb on - strand, within MMJJ_RS01525 at 298.553 kb on - strand, within MMJJ_RS01525 at 298.554 kb on + strand, within MMJJ_RS01525 at 298.555 kb on - strand, within MMJJ_RS01525 at 298.555 kb on - strand, within MMJJ_RS01525 at 298.556 kb on + strand, within MMJJ_RS01525 at 298.558 kb on - strand, within MMJJ_RS01525 at 298.558 kb on - strand, within MMJJ_RS01525 at 298.559 kb on + strand, within MMJJ_RS01525 at 298.561 kb on - strand, within MMJJ_RS01525 at 298.563 kb on - strand, within MMJJ_RS01525 at 298.563 kb on - strand, within MMJJ_RS01525
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 296,248 + MMJJ_RS01510 0.39 -0.3 296,265 - +1.1 296,265 - MMJJ_RS01510 0.40 +2.6 296,304 - MMJJ_RS01510 0.43 -2.2 296,337 - MMJJ_RS01510 0.45 +0.8 296,378 + MMJJ_RS01510 0.48 +0.4 296,384 + MMJJ_RS01510 0.49 +0.5 296,409 - MMJJ_RS01510 0.51 +0.6 296,443 + MMJJ_RS01510 0.53 -0.8 296,451 + MMJJ_RS01510 0.54 +1.3 296,463 - MMJJ_RS01510 0.54 -1.2 296,501 - MMJJ_RS01510 0.57 +0.8 296,501 - MMJJ_RS01510 0.57 +1.1 296,586 + MMJJ_RS01510 0.63 +0.3 296,653 - MMJJ_RS01510 0.68 -0.5 296,712 + MMJJ_RS01510 0.72 +1.9 296,744 - MMJJ_RS01510 0.75 -0.5 296,874 - MMJJ_RS01510 0.84 -0.4 296,916 - MMJJ_RS01510 0.87 +1.7 297,015 + -1.7 297,066 - +1.9 297,069 + -1.1 297,073 - -0.5 297,074 + +1.7 297,076 + +0.8 297,088 - -0.5 297,107 + -3.3 297,115 - -3.6 297,120 - +2.7 297,121 + -1.6 297,126 + -2.8 297,126 + -0.3 297,126 + +0.1 297,129 + +2.9 297,195 + -1.5 297,208 - +2.3 297,254 - +1.9 297,259 - -1.3 297,273 - MMJJ_RS01515 0.11 +2.0 297,274 + MMJJ_RS01515 0.11 +4.0 297,321 + MMJJ_RS01515 0.24 -0.5 297,343 + MMJJ_RS01515 0.29 -2.8 297,370 - MMJJ_RS01515 0.36 -3.2 297,371 + MMJJ_RS01515 0.36 -1.5 297,394 + MMJJ_RS01515 0.42 -2.8 297,394 + MMJJ_RS01515 0.42 -1.7 297,504 + MMJJ_RS01515 0.71 +3.1 297,504 + MMJJ_RS01515 0.71 +3.7 297,552 - MMJJ_RS01515 0.83 +2.4 297,567 - MMJJ_RS01515 0.87 +1.4 297,567 - MMJJ_RS01515 0.87 +2.0 297,588 - +0.0 297,588 - +1.3 297,588 - -1.8 297,588 - +2.6 297,588 - -2.9 297,589 + -3.5 297,589 + -0.5 297,589 + -0.9 297,589 + +1.5 297,589 + +2.1 297,589 + -1.2 297,589 + +0.1 297,641 - +0.7 297,651 + MMJJ_RS01520 0.12 -0.5 297,717 + MMJJ_RS01520 0.44 -1.7 297,757 - MMJJ_RS01520 0.64 -0.1 297,757 - MMJJ_RS01520 0.64 -1.9 297,779 + MMJJ_RS01520 0.75 -1.1 297,802 - MMJJ_RS01520 0.86 -2.6 297,802 - MMJJ_RS01520 0.86 -1.6 297,807 + MMJJ_RS01520 0.88 +2.9 297,842 - +0.1 297,864 + +0.5 297,901 - -0.0 297,906 - +3.5 297,965 - MMJJ_RS01525 0.13 -2.3 297,965 - MMJJ_RS01525 0.13 +1.4 297,976 - MMJJ_RS01525 0.14 -1.8 297,976 - MMJJ_RS01525 0.14 -0.7 297,977 + MMJJ_RS01525 0.14 +1.1 298,059 - MMJJ_RS01525 0.23 -2.3 298,124 - MMJJ_RS01525 0.30 +3.1 298,129 - MMJJ_RS01525 0.31 -1.7 298,130 + MMJJ_RS01525 0.31 -2.4 298,130 + MMJJ_RS01525 0.31 +2.2 298,135 - MMJJ_RS01525 0.31 -1.5 298,141 - MMJJ_RS01525 0.32 -4.0 298,142 + MMJJ_RS01525 0.32 +0.1 298,142 + MMJJ_RS01525 0.32 +2.4 298,186 - MMJJ_RS01525 0.37 -3.3 298,217 + MMJJ_RS01525 0.40 +3.5 298,218 - MMJJ_RS01525 0.40 +0.7 298,250 + MMJJ_RS01525 0.44 +4.0 298,361 - MMJJ_RS01525 0.56 +2.4 298,362 + MMJJ_RS01525 0.56 +4.5 298,408 - MMJJ_RS01525 0.61 +2.2 298,412 + MMJJ_RS01525 0.62 +2.2 298,412 + MMJJ_RS01525 0.62 +1.7 298,412 + MMJJ_RS01525 0.62 +0.6 298,430 + MMJJ_RS01525 0.64 +0.3 298,435 - MMJJ_RS01525 0.64 -1.5 298,435 - MMJJ_RS01525 0.64 +0.5 298,436 + MMJJ_RS01525 0.64 -1.3 298,436 + MMJJ_RS01525 0.64 -0.7 298,437 - MMJJ_RS01525 0.64 -0.5 298,443 + MMJJ_RS01525 0.65 +3.3 298,500 + MMJJ_RS01525 0.71 +1.4 298,500 + MMJJ_RS01525 0.71 -1.2 298,501 - +0.1 298,501 - MMJJ_RS01525 0.71 +2.1 298,553 - MMJJ_RS01525 0.77 +0.6 298,554 + MMJJ_RS01525 0.77 +0.1 298,555 - MMJJ_RS01525 0.77 +5.0 298,555 - MMJJ_RS01525 0.77 +2.1 298,556 + MMJJ_RS01525 0.77 +1.0 298,558 - MMJJ_RS01525 0.77 +1.2 298,558 - MMJJ_RS01525 0.77 +1.3 298,559 + MMJJ_RS01525 0.78 +2.4 298,561 - MMJJ_RS01525 0.78 +1.8 298,563 - MMJJ_RS01525 0.78 +3.7 298,563 - MMJJ_RS01525 0.78 +1.7
Or see this region's nucleotide sequence