Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01445 and MMJJ_RS01450 are separated by 79 nucleotides MMJJ_RS01450 and MMJJ_RS01455 are separated by 164 nucleotides MMJJ_RS01455 and MMJJ_RS01460 are separated by 78 nucleotides
MMJJ_RS01445: MMJJ_RS01445 - O-phosphoserine--tRNA ligase, at 281,949 to 283,562
_RS01445
MMJJ_RS01450: MMJJ_RS01450 - iron ABC transporter substrate-binding protein, at 283,642 to 284,769
_RS01450
MMJJ_RS01455: MMJJ_RS01455 - triose-phosphate isomerase, at 284,934 to 285,599
_RS01455
MMJJ_RS01460: MMJJ_RS01460 - 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase, at 285,678 to 286,496
_RS01460
Position (kb)
283
284
285 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 282.677 kb on - strand, within MMJJ_RS01445 at 282.887 kb on + strand, within MMJJ_RS01445 at 283.590 kb on - strand at 283.591 kb on + strand at 283.601 kb on - strand at 283.601 kb on - strand at 283.627 kb on - strand at 283.643 kb on - strand at 283.643 kb on - strand at 283.644 kb on + strand at 283.660 kb on + strand at 283.709 kb on - strand at 283.734 kb on - strand at 283.735 kb on + strand at 283.736 kb on - strand at 283.736 kb on - strand at 283.736 kb on - strand at 283.737 kb on + strand at 283.737 kb on + strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.740 kb on - strand at 283.741 kb on + strand at 283.765 kb on - strand, within MMJJ_RS01450 at 283.787 kb on - strand, within MMJJ_RS01450 at 283.789 kb on - strand, within MMJJ_RS01450 at 283.804 kb on + strand, within MMJJ_RS01450 at 283.804 kb on + strand, within MMJJ_RS01450 at 283.806 kb on + strand, within MMJJ_RS01450 at 283.806 kb on + strand, within MMJJ_RS01450 at 283.806 kb on + strand, within MMJJ_RS01450 at 283.806 kb on + strand, within MMJJ_RS01450 at 283.834 kb on + strand, within MMJJ_RS01450 at 283.834 kb on + strand, within MMJJ_RS01450 at 283.838 kb on + strand, within MMJJ_RS01450 at 283.842 kb on - strand, within MMJJ_RS01450 at 283.844 kb on - strand, within MMJJ_RS01450 at 283.845 kb on + strand, within MMJJ_RS01450 at 283.852 kb on + strand, within MMJJ_RS01450 at 283.852 kb on + strand, within MMJJ_RS01450 at 283.873 kb on + strand, within MMJJ_RS01450 at 283.893 kb on - strand, within MMJJ_RS01450 at 283.894 kb on + strand, within MMJJ_RS01450 at 283.902 kb on - strand, within MMJJ_RS01450 at 283.926 kb on + strand, within MMJJ_RS01450 at 283.936 kb on - strand, within MMJJ_RS01450 at 283.937 kb on + strand, within MMJJ_RS01450 at 283.960 kb on - strand, within MMJJ_RS01450 at 284.221 kb on - strand, within MMJJ_RS01450 at 284.222 kb on + strand at 284.222 kb on + strand, within MMJJ_RS01450 at 284.222 kb on + strand, within MMJJ_RS01450 at 284.236 kb on + strand, within MMJJ_RS01450 at 284.279 kb on - strand, within MMJJ_RS01450 at 284.282 kb on + strand, within MMJJ_RS01450 at 284.321 kb on - strand, within MMJJ_RS01450 at 284.387 kb on - strand, within MMJJ_RS01450 at 284.387 kb on - strand, within MMJJ_RS01450 at 284.388 kb on + strand, within MMJJ_RS01450 at 284.388 kb on + strand, within MMJJ_RS01450 at 284.388 kb on + strand, within MMJJ_RS01450 at 284.391 kb on - strand, within MMJJ_RS01450 at 284.401 kb on - strand, within MMJJ_RS01450 at 284.401 kb on - strand, within MMJJ_RS01450 at 284.402 kb on + strand, within MMJJ_RS01450 at 284.413 kb on + strand, within MMJJ_RS01450 at 284.464 kb on - strand, within MMJJ_RS01450 at 284.465 kb on + strand, within MMJJ_RS01450 at 284.467 kb on - strand, within MMJJ_RS01450 at 284.467 kb on - strand, within MMJJ_RS01450 at 284.505 kb on - strand, within MMJJ_RS01450 at 284.506 kb on + strand, within MMJJ_RS01450 at 284.506 kb on + strand, within MMJJ_RS01450 at 284.508 kb on + strand, within MMJJ_RS01450 at 284.556 kb on - strand, within MMJJ_RS01450 at 284.556 kb on - strand, within MMJJ_RS01450 at 284.556 kb on - strand, within MMJJ_RS01450 at 284.557 kb on + strand, within MMJJ_RS01450 at 284.569 kb on - strand, within MMJJ_RS01450 at 284.569 kb on - strand, within MMJJ_RS01450 at 284.570 kb on + strand, within MMJJ_RS01450 at 284.570 kb on + strand, within MMJJ_RS01450 at 284.570 kb on + strand, within MMJJ_RS01450 at 284.573 kb on - strand, within MMJJ_RS01450 at 284.608 kb on - strand, within MMJJ_RS01450 at 284.608 kb on - strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.617 kb on + strand, within MMJJ_RS01450 at 284.655 kb on - strand, within MMJJ_RS01450 at 284.664 kb on - strand at 284.677 kb on + strand at 284.697 kb on - strand at 284.698 kb on + strand at 284.709 kb on - strand at 284.769 kb on - strand at 284.770 kb on + strand at 284.796 kb on - strand at 284.824 kb on + strand at 284.837 kb on - strand at 284.842 kb on + strand at 284.878 kb on - strand at 284.929 kb on - strand at 285.481 kb on + strand, within MMJJ_RS01455 at 285.574 kb on - strand at 285.586 kb on + strand at 285.599 kb on - strand at 285.619 kb on + strand at 285.620 kb on - strand at 285.624 kb on + strand at 285.644 kb on - strand at 285.649 kb on + strand at 285.649 kb on + strand at 285.649 kb on + strand at 285.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 282,677 - MMJJ_RS01445 0.45 +1.1 282,887 + MMJJ_RS01445 0.58 -0.5 283,590 - -1.9 283,591 + -0.9 283,601 - +0.8 283,601 - -0.6 283,627 - +4.5 283,643 - -0.2 283,643 - +1.1 283,644 + -0.8 283,660 + +2.4 283,709 - +3.0 283,734 - +1.8 283,735 + -2.5 283,736 - +1.4 283,736 - -3.5 283,736 - +1.2 283,737 + -2.5 283,737 + +2.2 283,738 - -0.1 283,738 - +0.4 283,738 - -1.5 283,738 - -1.0 283,738 - +2.7 283,738 - -0.2 283,738 - -1.6 283,739 + +0.4 283,739 + -0.7 283,739 + +0.5 283,739 + -2.7 283,739 + +3.1 283,739 + -1.2 283,739 + +0.5 283,739 + +4.0 283,739 + -2.2 283,739 + +2.1 283,740 - +0.2 283,741 + +1.1 283,765 - MMJJ_RS01450 0.11 +0.2 283,787 - MMJJ_RS01450 0.13 -2.2 283,789 - MMJJ_RS01450 0.13 -0.4 283,804 + MMJJ_RS01450 0.14 +1.0 283,804 + MMJJ_RS01450 0.14 -0.8 283,806 + MMJJ_RS01450 0.15 +1.7 283,806 + MMJJ_RS01450 0.15 -0.0 283,806 + MMJJ_RS01450 0.15 +0.9 283,806 + MMJJ_RS01450 0.15 +2.8 283,834 + MMJJ_RS01450 0.17 +0.5 283,834 + MMJJ_RS01450 0.17 +0.8 283,838 + MMJJ_RS01450 0.17 +1.8 283,842 - MMJJ_RS01450 0.18 +0.3 283,844 - MMJJ_RS01450 0.18 +2.0 283,845 + MMJJ_RS01450 0.18 +0.4 283,852 + MMJJ_RS01450 0.19 +1.4 283,852 + MMJJ_RS01450 0.19 -0.3 283,873 + MMJJ_RS01450 0.20 +0.9 283,893 - MMJJ_RS01450 0.22 +1.0 283,894 + MMJJ_RS01450 0.22 +1.4 283,902 - MMJJ_RS01450 0.23 -0.8 283,926 + MMJJ_RS01450 0.25 -0.5 283,936 - MMJJ_RS01450 0.26 -1.7 283,937 + MMJJ_RS01450 0.26 -1.3 283,960 - MMJJ_RS01450 0.28 -0.6 284,221 - MMJJ_RS01450 0.51 +0.8 284,222 + +1.1 284,222 + MMJJ_RS01450 0.51 -2.8 284,222 + MMJJ_RS01450 0.51 -0.2 284,236 + MMJJ_RS01450 0.53 +1.8 284,279 - MMJJ_RS01450 0.56 -0.1 284,282 + MMJJ_RS01450 0.57 +2.3 284,321 - MMJJ_RS01450 0.60 -1.4 284,387 - MMJJ_RS01450 0.66 -3.2 284,387 - MMJJ_RS01450 0.66 +1.1 284,388 + MMJJ_RS01450 0.66 -1.3 284,388 + MMJJ_RS01450 0.66 -3.0 284,388 + MMJJ_RS01450 0.66 +0.1 284,391 - MMJJ_RS01450 0.66 +0.6 284,401 - MMJJ_RS01450 0.67 -1.7 284,401 - MMJJ_RS01450 0.67 -1.2 284,402 + MMJJ_RS01450 0.67 -0.5 284,413 + MMJJ_RS01450 0.68 -2.9 284,464 - MMJJ_RS01450 0.73 -0.2 284,465 + MMJJ_RS01450 0.73 +2.0 284,467 - MMJJ_RS01450 0.73 -1.4 284,467 - MMJJ_RS01450 0.73 +2.2 284,505 - MMJJ_RS01450 0.77 +0.4 284,506 + MMJJ_RS01450 0.77 -0.7 284,506 + MMJJ_RS01450 0.77 +2.6 284,508 + MMJJ_RS01450 0.77 +0.4 284,556 - MMJJ_RS01450 0.81 -2.1 284,556 - MMJJ_RS01450 0.81 +2.4 284,556 - MMJJ_RS01450 0.81 -1.7 284,557 + MMJJ_RS01450 0.81 -2.5 284,569 - MMJJ_RS01450 0.82 +0.3 284,569 - MMJJ_RS01450 0.82 -0.3 284,570 + MMJJ_RS01450 0.82 -2.3 284,570 + MMJJ_RS01450 0.82 -0.7 284,570 + MMJJ_RS01450 0.82 +2.5 284,573 - MMJJ_RS01450 0.83 -2.7 284,608 - MMJJ_RS01450 0.86 +4.5 284,608 - MMJJ_RS01450 0.86 -0.3 284,609 + MMJJ_RS01450 0.86 +0.9 284,609 + MMJJ_RS01450 0.86 +0.5 284,609 + MMJJ_RS01450 0.86 -0.2 284,609 + MMJJ_RS01450 0.86 +1.0 284,609 + MMJJ_RS01450 0.86 -1.2 284,609 + MMJJ_RS01450 0.86 +1.3 284,609 + MMJJ_RS01450 0.86 +1.4 284,617 + MMJJ_RS01450 0.86 -0.9 284,655 - MMJJ_RS01450 0.90 -1.1 284,664 - -0.4 284,677 + -1.6 284,697 - +1.1 284,698 + +1.1 284,709 - +1.2 284,769 - +0.1 284,770 + +1.4 284,796 - +2.1 284,824 + -0.7 284,837 - +1.2 284,842 + -3.1 284,878 - +0.9 284,929 - +0.3 285,481 + MMJJ_RS01455 0.82 -0.9 285,574 - -2.4 285,586 + -0.5 285,599 - +0.1 285,619 + +1.1 285,620 - -1.7 285,624 + +0.8 285,644 - -3.6 285,649 + +2.9 285,649 + -1.2 285,649 + +3.8 285,696 - +1.6
Or see this region's nucleotide sequence