Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01400 and MMJJ_RS01405 are separated by 262 nucleotides MMJJ_RS01405 and MMJJ_RS01410 are separated by 95 nucleotides MMJJ_RS01410 and MMJJ_RS01415 are separated by 134 nucleotides
MMJJ_RS01400: MMJJ_RS01400 - leucine--tRNA ligase, at 269,866 to 272,733
_RS01400
MMJJ_RS01405: MMJJ_RS01405 - proline--tRNA ligase, at 272,996 to 274,378
_RS01405
MMJJ_RS01410: MMJJ_RS01410 - archaeal proteasome endopeptidase complex subunit beta, at 274,474 to 275,133
_RS01410
MMJJ_RS01415: MMJJ_RS01415 - beta-CASP ribonuclease aCPSF1, at 275,268 to 277,175
_RS01415
Position (kb) 272
273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 272.811 kb on + strand at 272.826 kb on + strand at 272.865 kb on + strand at 272.867 kb on + strand at 272.874 kb on + strand at 272.874 kb on + strand at 272.874 kb on + strand at 272.874 kb on + strand at 272.878 kb on + strand at 272.878 kb on + strand at 272.891 kb on + strand at 272.891 kb on + strand at 272.898 kb on - strand at 272.898 kb on - strand at 272.899 kb on + strand at 272.914 kb on + strand at 272.914 kb on + strand at 272.940 kb on + strand at 272.975 kb on - strand at 273.318 kb on + strand at 273.318 kb on + strand, within MMJJ_RS01405 at 273.318 kb on + strand, within MMJJ_RS01405 at 274.376 kb on - strand at 274.376 kb on - strand at 274.376 kb on - strand at 274.376 kb on - strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.414 kb on + strand at 274.428 kb on + strand at 274.428 kb on + strand at 274.428 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.441 kb on + strand at 274.445 kb on - strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.457 kb on - strand at 274.458 kb on + strand at 274.460 kb on - strand at 274.460 kb on - strand at 274.460 kb on - strand at 274.461 kb on + strand at 274.471 kb on + strand at 274.493 kb on + strand at 274.504 kb on - strand at 274.504 kb on - strand at 274.504 kb on - strand at 274.505 kb on + strand at 274.505 kb on + strand at 274.505 kb on + strand at 274.532 kb on - strand at 274.532 kb on - strand at 274.557 kb on + strand, within MMJJ_RS01410 at 274.557 kb on + strand, within MMJJ_RS01410 at 274.619 kb on + strand, within MMJJ_RS01410 at 274.619 kb on + strand, within MMJJ_RS01410 at 274.621 kb on + strand, within MMJJ_RS01410 at 274.621 kb on + strand, within MMJJ_RS01410 at 274.633 kb on - strand, within MMJJ_RS01410 at 274.638 kb on + strand, within MMJJ_RS01410 at 274.647 kb on - strand, within MMJJ_RS01410 at 274.649 kb on - strand, within MMJJ_RS01410 at 274.650 kb on + strand, within MMJJ_RS01410 at 274.650 kb on + strand, within MMJJ_RS01410 at 274.735 kb on + strand, within MMJJ_RS01410 at 274.760 kb on - strand, within MMJJ_RS01410 at 274.767 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 275.026 kb on - strand, within MMJJ_RS01410 at 275.232 kb on + strand at 275.249 kb on + strand at 275.368 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 272,811 + -1.3 272,826 + +1.1 272,865 + -0.7 272,867 + +0.2 272,874 + -1.5 272,874 + -1.5 272,874 + -2.9 272,874 + -2.3 272,878 + +0.1 272,878 + -2.7 272,891 + -0.9 272,891 + -1.5 272,898 - -0.9 272,898 - -0.5 272,899 + +1.1 272,914 + -0.3 272,914 + +0.5 272,940 + -1.0 272,975 - -0.5 273,318 + -1.5 273,318 + MMJJ_RS01405 0.23 -0.8 273,318 + MMJJ_RS01405 0.23 -0.7 274,376 - +1.6 274,376 - +1.5 274,376 - +2.1 274,376 - -0.4 274,377 + -2.1 274,377 + +1.1 274,377 + -0.8 274,377 + +1.7 274,377 + +0.5 274,414 + +0.4 274,428 + +3.0 274,428 + -1.3 274,428 + -2.8 274,430 + +1.7 274,430 + -2.6 274,430 + -1.7 274,430 + -2.2 274,441 + -1.3 274,445 - +1.5 274,446 + -0.7 274,446 + +0.1 274,446 + -1.3 274,446 + +3.0 274,457 - -1.7 274,458 + -0.2 274,460 - -1.0 274,460 - -0.2 274,460 - -3.3 274,461 + -1.3 274,471 + -3.6 274,493 + -0.0 274,504 - -1.7 274,504 - -1.6 274,504 - -1.6 274,505 + -2.8 274,505 + -2.3 274,505 + -2.3 274,532 - +0.5 274,532 - +0.9 274,557 + MMJJ_RS01410 0.13 -0.5 274,557 + MMJJ_RS01410 0.13 -2.0 274,619 + MMJJ_RS01410 0.22 -0.9 274,619 + MMJJ_RS01410 0.22 -0.4 274,621 + MMJJ_RS01410 0.22 -1.3 274,621 + MMJJ_RS01410 0.22 +0.1 274,633 - MMJJ_RS01410 0.24 -1.3 274,638 + MMJJ_RS01410 0.25 +1.1 274,647 - MMJJ_RS01410 0.26 +1.1 274,649 - MMJJ_RS01410 0.27 -0.4 274,650 + MMJJ_RS01410 0.27 -0.5 274,650 + MMJJ_RS01410 0.27 -1.3 274,735 + MMJJ_RS01410 0.40 -0.7 274,760 - MMJJ_RS01410 0.43 -0.5 274,767 - MMJJ_RS01410 0.44 +1.1 274,811 - MMJJ_RS01410 0.51 -0.9 274,811 - MMJJ_RS01410 0.51 -2.1 274,811 - MMJJ_RS01410 0.51 -0.9 275,026 - MMJJ_RS01410 0.84 -0.9 275,232 + +1.1 275,249 + -0.9 275,368 - -0.5
Or see this region's nucleotide sequence