Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01355 and MMJJ_RS01360 are separated by 4 nucleotides MMJJ_RS01360 and MMJJ_RS01365 are separated by 92 nucleotides
MMJJ_RS01355: MMJJ_RS01355 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 261,551 to 262,834
_RS01355
MMJJ_RS01360: MMJJ_RS01360 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 262,839 to 263,939
_RS01360
MMJJ_RS01365: MMJJ_RS01365 - Mrp/NBP35 family ATP-binding protein, at 264,032 to 264,901
_RS01365
Position (kb) 262
263
264 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 261.921 kb on - strand, within MMJJ_RS01355 at 262.135 kb on + strand, within MMJJ_RS01355 at 262.137 kb on + strand at 262.139 kb on + strand, within MMJJ_RS01355 at 262.336 kb on + strand, within MMJJ_RS01355 at 262.357 kb on + strand at 262.589 kb on - strand, within MMJJ_RS01355 at 262.589 kb on - strand, within MMJJ_RS01355 at 262.590 kb on + strand, within MMJJ_RS01355 at 262.771 kb on + strand at 262.777 kb on - strand at 262.914 kb on + strand at 263.104 kb on - strand, within MMJJ_RS01360 at 263.518 kb on - strand, within MMJJ_RS01360 at 263.723 kb on - strand, within MMJJ_RS01360 at 263.723 kb on - strand, within MMJJ_RS01360 at 263.724 kb on + strand, within MMJJ_RS01360 at 263.801 kb on + strand, within MMJJ_RS01360 at 263.948 kb on - strand at 263.950 kb on - strand at 263.950 kb on - strand at 263.951 kb on + strand at 263.978 kb on - strand at 263.978 kb on - strand at 263.978 kb on - strand at 263.979 kb on + strand at 263.979 kb on + strand at 263.981 kb on - strand at 263.981 kb on - strand at 263.987 kb on + strand at 263.993 kb on - strand at 263.993 kb on - strand at 263.994 kb on + strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.996 kb on + strand at 263.996 kb on + strand at 263.996 kb on + strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.998 kb on + strand at 263.998 kb on + strand at 263.998 kb on + strand at 264.004 kb on - strand at 264.005 kb on + strand at 264.009 kb on + strand at 264.012 kb on - strand at 264.012 kb on - strand at 264.061 kb on - strand at 264.136 kb on - strand, within MMJJ_RS01365 at 264.152 kb on - strand, within MMJJ_RS01365 at 264.255 kb on - strand, within MMJJ_RS01365 at 264.336 kb on - strand, within MMJJ_RS01365 at 264.338 kb on - strand, within MMJJ_RS01365 at 264.338 kb on - strand, within MMJJ_RS01365 at 264.384 kb on + strand, within MMJJ_RS01365 at 264.412 kb on - strand, within MMJJ_RS01365 at 264.414 kb on - strand, within MMJJ_RS01365 at 264.489 kb on - strand, within MMJJ_RS01365 at 264.520 kb on - strand, within MMJJ_RS01365 at 264.591 kb on - strand, within MMJJ_RS01365 at 264.618 kb on - strand, within MMJJ_RS01365 at 264.655 kb on - strand, within MMJJ_RS01365 at 264.681 kb on - strand, within MMJJ_RS01365 at 264.709 kb on - strand, within MMJJ_RS01365 at 264.750 kb on - strand, within MMJJ_RS01365 at 264.827 kb on + strand at 264.838 kb on - strand at 264.899 kb on - strand at 264.899 kb on - strand at 264.904 kb on - strand at 264.938 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 261,921 - MMJJ_RS01355 0.29 -0.5 262,135 + MMJJ_RS01355 0.45 -0.5 262,137 + -0.3 262,139 + MMJJ_RS01355 0.46 -0.5 262,336 + MMJJ_RS01355 0.61 -0.7 262,357 + +1.1 262,589 - MMJJ_RS01355 0.81 -1.5 262,589 - MMJJ_RS01355 0.81 +0.1 262,590 + MMJJ_RS01355 0.81 -1.3 262,771 + -2.1 262,777 - -0.5 262,914 + +0.1 263,104 - MMJJ_RS01360 0.24 -2.1 263,518 - MMJJ_RS01360 0.62 +0.1 263,723 - MMJJ_RS01360 0.80 -1.3 263,723 - MMJJ_RS01360 0.80 -1.7 263,724 + MMJJ_RS01360 0.80 -0.5 263,801 + MMJJ_RS01360 0.87 +1.1 263,948 - +1.7 263,950 - -0.5 263,950 - -2.1 263,951 + -2.6 263,978 - -0.5 263,978 - -0.5 263,978 - -1.5 263,979 + -1.4 263,979 + +0.1 263,981 - -0.2 263,981 - +1.3 263,987 + -0.1 263,993 - -2.1 263,993 - +3.6 263,994 + -0.1 263,995 - -2.2 263,995 - +4.6 263,995 - +0.7 263,995 - -0.7 263,996 + -2.2 263,996 + +1.2 263,996 + -1.9 263,997 - +0.3 263,997 - -3.1 263,997 - -1.6 263,997 - -0.2 263,998 + +2.1 263,998 + -1.1 263,998 + -3.5 264,004 - +0.1 264,005 + +0.8 264,009 + +1.1 264,012 - -1.5 264,012 - +1.1 264,061 - +1.7 264,136 - MMJJ_RS01365 0.12 -2.7 264,152 - MMJJ_RS01365 0.14 -3.7 264,255 - MMJJ_RS01365 0.26 -1.4 264,336 - MMJJ_RS01365 0.35 -0.4 264,338 - MMJJ_RS01365 0.35 -1.8 264,338 - MMJJ_RS01365 0.35 +0.3 264,384 + MMJJ_RS01365 0.40 -1.4 264,412 - MMJJ_RS01365 0.44 +0.2 264,414 - MMJJ_RS01365 0.44 -3.7 264,489 - MMJJ_RS01365 0.53 -0.9 264,520 - MMJJ_RS01365 0.56 +0.7 264,591 - MMJJ_RS01365 0.64 +2.2 264,618 - MMJJ_RS01365 0.67 +1.2 264,655 - MMJJ_RS01365 0.72 -0.8 264,681 - MMJJ_RS01365 0.75 +0.7 264,709 - MMJJ_RS01365 0.78 -1.4 264,750 - MMJJ_RS01365 0.83 -0.5 264,827 + +0.8 264,838 - +3.5 264,899 - -1.5 264,899 - -1.8 264,904 - -1.9 264,938 + -0.6
Or see this region's nucleotide sequence