Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01245 and MMJJ_RS01250 are separated by 36 nucleotides MMJJ_RS01250 and MMJJ_RS01255 are separated by 350 nucleotides MMJJ_RS01255 and MMJJ_RS01260 are separated by 8 nucleotides MMJJ_RS01260 and MMJJ_RS01265 are separated by 228 nucleotides MMJJ_RS01265 and MMJJ_RS01270 are separated by 17 nucleotides
MMJJ_RS01245: MMJJ_RS01245 - excinuclease ABC subunit UvrB, at 240,688 to 242,628
_RS01245
MMJJ_RS01250: MMJJ_RS01250 - hypothetical protein, at 242,665 to 242,898
_RS01250
MMJJ_RS01255: MMJJ_RS01255 - YqhA family protein, at 243,249 to 243,806
_RS01255
MMJJ_RS01260: MMJJ_RS01260 - hypothetical protein, at 243,815 to 244,159
_RS01260
MMJJ_RS01265: MMJJ_RS01265 - cupin domain-containing protein, at 244,388 to 244,732
_RS01265
MMJJ_RS01270: MMJJ_RS01270 - 4Fe-4S binding protein, at 244,750 to 245,382
_RS01270
Position (kb)
243
244 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 242.331 kb on + strand, within MMJJ_RS01245 at 242.361 kb on - strand, within MMJJ_RS01245 at 242.447 kb on - strand at 242.468 kb on - strand at 242.486 kb on - strand at 242.545 kb on - strand at 242.546 kb on + strand at 242.631 kb on - strand at 242.649 kb on - strand at 242.678 kb on - strand at 242.679 kb on + strand at 242.693 kb on + strand, within MMJJ_RS01250 at 242.698 kb on - strand, within MMJJ_RS01250 at 242.719 kb on + strand, within MMJJ_RS01250 at 242.719 kb on + strand, within MMJJ_RS01250 at 242.731 kb on - strand, within MMJJ_RS01250 at 242.732 kb on + strand, within MMJJ_RS01250 at 242.732 kb on + strand, within MMJJ_RS01250 at 242.849 kb on - strand, within MMJJ_RS01250 at 242.849 kb on - strand, within MMJJ_RS01250 at 242.849 kb on - strand, within MMJJ_RS01250 at 242.854 kb on - strand, within MMJJ_RS01250 at 242.854 kb on - strand, within MMJJ_RS01250 at 242.890 kb on - strand at 242.891 kb on + strand at 242.920 kb on - strand at 242.934 kb on + strand at 242.937 kb on - strand at 242.940 kb on - strand at 242.940 kb on - strand at 242.941 kb on + strand at 242.941 kb on + strand at 242.942 kb on - strand at 242.943 kb on + strand at 242.943 kb on + strand at 242.945 kb on + strand at 242.945 kb on + strand at 242.945 kb on - strand at 242.966 kb on - strand at 242.966 kb on - strand at 242.966 kb on - strand at 242.969 kb on - strand at 242.969 kb on - strand at 242.969 kb on - strand at 242.970 kb on + strand at 242.971 kb on - strand at 242.972 kb on + strand at 242.986 kb on - strand at 243.019 kb on + strand at 243.025 kb on - strand at 243.025 kb on - strand at 243.026 kb on + strand at 243.030 kb on - strand at 243.030 kb on - strand at 243.031 kb on + strand at 243.031 kb on + strand at 243.036 kb on - strand at 243.037 kb on + strand at 243.103 kb on - strand at 243.103 kb on - strand at 243.103 kb on - strand at 243.118 kb on - strand at 243.121 kb on + strand at 243.121 kb on + strand at 243.121 kb on + strand at 243.123 kb on - strand at 243.123 kb on - strand at 243.124 kb on + strand at 243.124 kb on + strand at 243.124 kb on + strand at 243.124 kb on + strand at 243.147 kb on + strand at 243.160 kb on - strand at 243.165 kb on + strand at 243.168 kb on - strand at 243.169 kb on + strand at 243.169 kb on + strand at 243.173 kb on - strand at 243.174 kb on + strand at 243.177 kb on - strand at 243.177 kb on - strand at 243.178 kb on + strand at 243.214 kb on - strand at 243.217 kb on + strand at 243.224 kb on - strand at 243.225 kb on + strand at 243.236 kb on - strand at 243.236 kb on - strand at 243.248 kb on - strand at 243.293 kb on - strand at 243.296 kb on - strand at 243.296 kb on - strand at 243.297 kb on + strand at 243.353 kb on - strand, within MMJJ_RS01255 at 243.395 kb on - strand, within MMJJ_RS01255 at 243.395 kb on - strand, within MMJJ_RS01255 at 243.441 kb on + strand, within MMJJ_RS01255 at 243.459 kb on + strand, within MMJJ_RS01255 at 243.485 kb on + strand, within MMJJ_RS01255 at 243.487 kb on - strand, within MMJJ_RS01255 at 243.509 kb on + strand, within MMJJ_RS01255 at 243.520 kb on - strand, within MMJJ_RS01255 at 243.523 kb on - strand, within MMJJ_RS01255 at 243.532 kb on - strand, within MMJJ_RS01255 at 243.533 kb on + strand, within MMJJ_RS01255 at 243.545 kb on - strand, within MMJJ_RS01255 at 243.559 kb on + strand, within MMJJ_RS01255 at 243.559 kb on + strand, within MMJJ_RS01255 at 243.580 kb on - strand, within MMJJ_RS01255 at 243.615 kb on + strand, within MMJJ_RS01255 at 243.628 kb on + strand, within MMJJ_RS01255 at 243.629 kb on - strand, within MMJJ_RS01255 at 243.629 kb on - strand, within MMJJ_RS01255 at 243.665 kb on - strand, within MMJJ_RS01255 at 243.667 kb on - strand, within MMJJ_RS01255 at 243.668 kb on + strand, within MMJJ_RS01255 at 243.668 kb on + strand, within MMJJ_RS01255 at 243.671 kb on + strand, within MMJJ_RS01255 at 243.679 kb on - strand, within MMJJ_RS01255 at 243.679 kb on - strand, within MMJJ_RS01255 at 243.730 kb on - strand, within MMJJ_RS01255 at 243.734 kb on - strand, within MMJJ_RS01255 at 243.735 kb on + strand, within MMJJ_RS01255 at 243.739 kb on - strand, within MMJJ_RS01255 at 243.739 kb on - strand, within MMJJ_RS01255 at 243.740 kb on + strand, within MMJJ_RS01255 at 243.740 kb on + strand, within MMJJ_RS01255 at 243.740 kb on + strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.741 kb on - strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.742 kb on + strand, within MMJJ_RS01255 at 243.743 kb on - strand, within MMJJ_RS01255 at 243.743 kb on - strand, within MMJJ_RS01255 at 243.743 kb on - strand, within MMJJ_RS01255 at 243.744 kb on + strand, within MMJJ_RS01255 at 243.744 kb on + strand, within MMJJ_RS01255 at 243.805 kb on + strand at 243.820 kb on + strand at 243.820 kb on + strand at 243.834 kb on + strand at 243.863 kb on + strand, within MMJJ_RS01260 at 243.926 kb on - strand, within MMJJ_RS01260 at 243.927 kb on + strand, within MMJJ_RS01260 at 243.961 kb on - strand, within MMJJ_RS01260 at 243.962 kb on + strand, within MMJJ_RS01260 at 243.962 kb on + strand, within MMJJ_RS01260 at 243.962 kb on + strand, within MMJJ_RS01260 at 243.968 kb on + strand, within MMJJ_RS01260 at 243.971 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.989 kb on - strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.990 kb on + strand, within MMJJ_RS01260 at 243.991 kb on - strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 243.992 kb on + strand, within MMJJ_RS01260 at 244.021 kb on - strand, within MMJJ_RS01260 at 244.026 kb on - strand, within MMJJ_RS01260 at 244.038 kb on - strand, within MMJJ_RS01260 at 244.039 kb on + strand, within MMJJ_RS01260 at 244.039 kb on + strand, within MMJJ_RS01260 at 244.067 kb on + strand, within MMJJ_RS01260 at 244.084 kb on + strand, within MMJJ_RS01260 at 244.084 kb on + strand, within MMJJ_RS01260 at 244.204 kb on - strand at 244.204 kb on - strand at 244.212 kb on - strand at 244.213 kb on + strand at 244.219 kb on + strand at 244.239 kb on - strand at 244.265 kb on - strand at 244.303 kb on - strand at 244.308 kb on - strand at 244.309 kb on + strand at 244.331 kb on - strand at 244.331 kb on - strand at 244.332 kb on + strand at 244.332 kb on + strand at 244.332 kb on + strand at 244.332 kb on + strand at 244.332 kb on + strand at 244.333 kb on - strand at 244.334 kb on + strand at 244.338 kb on + strand at 244.347 kb on - strand at 244.347 kb on - strand at 244.348 kb on + strand at 244.352 kb on + strand at 244.354 kb on - strand at 244.373 kb on + strand at 244.375 kb on + strand at 244.397 kb on + strand at 244.397 kb on + strand at 244.397 kb on + strand at 244.397 kb on + strand at 244.506 kb on + strand, within MMJJ_RS01265 at 244.506 kb on + strand, within MMJJ_RS01265 at 244.506 kb on + strand, within MMJJ_RS01265 at 244.511 kb on - strand, within MMJJ_RS01265 at 244.583 kb on - strand, within MMJJ_RS01265 at 244.584 kb on + strand, within MMJJ_RS01265 at 244.644 kb on + strand, within MMJJ_RS01265 at 244.644 kb on + strand, within MMJJ_RS01265 at 244.652 kb on - strand, within MMJJ_RS01265 at 244.674 kb on + strand, within MMJJ_RS01265 at 244.674 kb on + strand, within MMJJ_RS01265 at 244.735 kb on - strand at 244.763 kb on - strand at 244.763 kb on - strand at 244.768 kb on - strand at 244.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 242,331 + MMJJ_RS01245 0.85 +1.3 242,361 - MMJJ_RS01245 0.86 -0.6 242,447 - -0.8 242,468 - -2.8 242,486 - -0.6 242,545 - +0.0 242,546 + -1.5 242,631 - -1.9 242,649 - +2.2 242,678 - +3.2 242,679 + -3.1 242,693 + MMJJ_RS01250 0.12 -0.7 242,698 - MMJJ_RS01250 0.14 -1.1 242,719 + MMJJ_RS01250 0.23 +3.9 242,719 + MMJJ_RS01250 0.23 -1.0 242,731 - MMJJ_RS01250 0.28 -0.6 242,732 + MMJJ_RS01250 0.29 -1.0 242,732 + MMJJ_RS01250 0.29 +0.3 242,849 - MMJJ_RS01250 0.79 -0.6 242,849 - MMJJ_RS01250 0.79 -1.7 242,849 - MMJJ_RS01250 0.79 -0.4 242,854 - MMJJ_RS01250 0.81 -3.3 242,854 - MMJJ_RS01250 0.81 +0.0 242,890 - -1.0 242,891 + -1.8 242,920 - +0.1 242,934 + +5.2 242,937 - -1.8 242,940 - +0.6 242,940 - -1.3 242,941 + -0.1 242,941 + -2.3 242,942 - -1.5 242,943 + -0.2 242,943 + -1.3 242,945 + -2.0 242,945 + +2.4 242,945 - +0.2 242,966 - +2.5 242,966 - -1.5 242,966 - +0.2 242,969 - +1.1 242,969 - +1.6 242,969 - +0.3 242,970 + -2.9 242,971 - -1.7 242,972 + +0.2 242,986 - -0.6 243,019 + +1.4 243,025 - -1.0 243,025 - +2.2 243,026 + +1.0 243,030 - +0.7 243,030 - +2.1 243,031 + +1.4 243,031 + +0.3 243,036 - +1.0 243,037 + +0.9 243,103 - -3.3 243,103 - +1.6 243,103 - -1.3 243,118 - -0.6 243,121 + +3.1 243,121 + -1.4 243,121 + -0.3 243,123 - -0.9 243,123 - -0.6 243,124 + -0.6 243,124 + -3.4 243,124 + -1.8 243,124 + +1.4 243,147 + -2.0 243,160 - -1.2 243,165 + +2.0 243,168 - +0.9 243,169 + +3.2 243,169 + +0.0 243,173 - -0.4 243,174 + +2.5 243,177 - +2.2 243,177 - -2.3 243,178 + -1.6 243,214 - +3.4 243,217 + +1.4 243,224 - -2.4 243,225 + -0.3 243,236 - +2.9 243,236 - +1.0 243,248 - -0.4 243,293 - +3.5 243,296 - -1.0 243,296 - -2.6 243,297 + +2.3 243,353 - MMJJ_RS01255 0.19 -0.5 243,395 - MMJJ_RS01255 0.26 -0.1 243,395 - MMJJ_RS01255 0.26 +1.0 243,441 + MMJJ_RS01255 0.34 +0.8 243,459 + MMJJ_RS01255 0.38 +3.0 243,485 + MMJJ_RS01255 0.42 +1.1 243,487 - MMJJ_RS01255 0.43 +1.4 243,509 + MMJJ_RS01255 0.47 +2.0 243,520 - MMJJ_RS01255 0.49 -0.2 243,523 - MMJJ_RS01255 0.49 -1.0 243,532 - MMJJ_RS01255 0.51 +4.4 243,533 + MMJJ_RS01255 0.51 -1.0 243,545 - MMJJ_RS01255 0.53 +2.6 243,559 + MMJJ_RS01255 0.56 +0.4 243,559 + MMJJ_RS01255 0.56 +0.1 243,580 - MMJJ_RS01255 0.59 +3.1 243,615 + MMJJ_RS01255 0.66 +0.7 243,628 + MMJJ_RS01255 0.68 -0.1 243,629 - MMJJ_RS01255 0.68 -1.6 243,629 - MMJJ_RS01255 0.68 +4.8 243,665 - MMJJ_RS01255 0.75 -2.6 243,667 - MMJJ_RS01255 0.75 +0.6 243,668 + MMJJ_RS01255 0.75 +1.5 243,668 + MMJJ_RS01255 0.75 +1.7 243,671 + MMJJ_RS01255 0.76 +1.3 243,679 - MMJJ_RS01255 0.77 +0.2 243,679 - MMJJ_RS01255 0.77 -0.8 243,730 - MMJJ_RS01255 0.86 -1.4 243,734 - MMJJ_RS01255 0.87 +1.0 243,735 + MMJJ_RS01255 0.87 +1.1 243,739 - MMJJ_RS01255 0.88 +2.9 243,739 - MMJJ_RS01255 0.88 +2.1 243,740 + MMJJ_RS01255 0.88 +3.6 243,740 + MMJJ_RS01255 0.88 +0.8 243,740 + MMJJ_RS01255 0.88 +0.9 243,741 - MMJJ_RS01255 0.88 -1.3 243,741 - MMJJ_RS01255 0.88 -1.3 243,741 - MMJJ_RS01255 0.88 -2.4 243,741 - MMJJ_RS01255 0.88 +1.1 243,741 - MMJJ_RS01255 0.88 -1.0 243,741 - MMJJ_RS01255 0.88 -1.2 243,741 - MMJJ_RS01255 0.88 -1.3 243,742 + MMJJ_RS01255 0.88 +0.4 243,742 + MMJJ_RS01255 0.88 -1.2 243,742 + MMJJ_RS01255 0.88 -0.6 243,742 + MMJJ_RS01255 0.88 -1.0 243,742 + MMJJ_RS01255 0.88 -2.1 243,742 + MMJJ_RS01255 0.88 +3.1 243,742 + MMJJ_RS01255 0.88 +3.3 243,742 + MMJJ_RS01255 0.88 -3.1 243,742 + MMJJ_RS01255 0.88 -0.1 243,742 + MMJJ_RS01255 0.88 +0.3 243,742 + MMJJ_RS01255 0.88 +1.0 243,742 + MMJJ_RS01255 0.88 +1.4 243,742 + MMJJ_RS01255 0.88 +1.1 243,742 + MMJJ_RS01255 0.88 -0.1 243,742 + MMJJ_RS01255 0.88 +1.9 243,743 - MMJJ_RS01255 0.89 +2.2 243,743 - MMJJ_RS01255 0.89 -1.3 243,743 - MMJJ_RS01255 0.89 +1.3 243,744 + MMJJ_RS01255 0.89 -0.2 243,744 + MMJJ_RS01255 0.89 -2.1 243,805 + +1.9 243,820 + -1.3 243,820 + +0.3 243,834 + +1.0 243,863 + MMJJ_RS01260 0.14 +0.5 243,926 - MMJJ_RS01260 0.32 -0.6 243,927 + MMJJ_RS01260 0.32 -2.0 243,961 - MMJJ_RS01260 0.42 -1.3 243,962 + MMJJ_RS01260 0.43 +1.4 243,962 + MMJJ_RS01260 0.43 -3.1 243,962 + MMJJ_RS01260 0.43 -1.6 243,968 + MMJJ_RS01260 0.44 -0.1 243,971 - MMJJ_RS01260 0.45 -1.4 243,989 - MMJJ_RS01260 0.50 -0.9 243,989 - MMJJ_RS01260 0.50 +0.7 243,989 - MMJJ_RS01260 0.50 +1.8 243,989 - MMJJ_RS01260 0.50 -0.6 243,989 - MMJJ_RS01260 0.50 -1.1 243,990 + MMJJ_RS01260 0.51 +1.5 243,990 + MMJJ_RS01260 0.51 +4.2 243,990 + MMJJ_RS01260 0.51 -0.1 243,990 + MMJJ_RS01260 0.51 +2.9 243,990 + MMJJ_RS01260 0.51 +1.0 243,990 + MMJJ_RS01260 0.51 -3.0 243,990 + MMJJ_RS01260 0.51 -1.0 243,990 + MMJJ_RS01260 0.51 +1.0 243,991 - MMJJ_RS01260 0.51 -2.8 243,992 + MMJJ_RS01260 0.51 -2.7 243,992 + MMJJ_RS01260 0.51 +0.7 243,992 + MMJJ_RS01260 0.51 +1.2 243,992 + MMJJ_RS01260 0.51 +1.9 243,992 + MMJJ_RS01260 0.51 -0.3 244,021 - MMJJ_RS01260 0.60 +2.5 244,026 - MMJJ_RS01260 0.61 -0.7 244,038 - MMJJ_RS01260 0.65 -1.0 244,039 + MMJJ_RS01260 0.65 +2.3 244,039 + MMJJ_RS01260 0.65 -3.7 244,067 + MMJJ_RS01260 0.73 -0.1 244,084 + MMJJ_RS01260 0.78 -1.8 244,084 + MMJJ_RS01260 0.78 +1.0 244,204 - +1.8 244,204 - -1.0 244,212 - -1.6 244,213 + -2.0 244,219 + +2.4 244,239 - -0.4 244,265 - +1.4 244,303 - -0.3 244,308 - +0.5 244,309 + -2.9 244,331 - +1.1 244,331 - -0.9 244,332 + -3.7 244,332 + +1.0 244,332 + -1.8 244,332 + +0.9 244,332 + -0.6 244,333 - -2.1 244,334 + +0.7 244,338 + +3.6 244,347 - +0.7 244,347 - -2.2 244,348 + +0.3 244,352 + -1.2 244,354 - +0.5 244,373 + -0.7 244,375 + +1.3 244,397 + +2.9 244,397 + +0.0 244,397 + +3.7 244,397 + -2.6 244,506 + MMJJ_RS01265 0.34 +1.5 244,506 + MMJJ_RS01265 0.34 -1.4 244,506 + MMJJ_RS01265 0.34 -1.8 244,511 - MMJJ_RS01265 0.36 +2.0 244,583 - MMJJ_RS01265 0.57 -0.5 244,584 + MMJJ_RS01265 0.57 +2.2 244,644 + MMJJ_RS01265 0.74 -0.4 244,644 + MMJJ_RS01265 0.74 -2.2 244,652 - MMJJ_RS01265 0.77 -2.9 244,674 + MMJJ_RS01265 0.83 +0.9 244,674 + MMJJ_RS01265 0.83 -0.7 244,735 - -2.8 244,763 - +0.3 244,763 - -1.3 244,768 - +0.3 244,789 - -2.2
Or see this region's nucleotide sequence