Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01115 and MMJJ_RS01120 are separated by 199 nucleotides MMJJ_RS01120 and MMJJ_RS01125 are separated by 9 nucleotides MMJJ_RS01125 and MMJJ_RS09255 are separated by 170 nucleotides MMJJ_RS09255 and MMJJ_RS01135 are separated by 64 nucleotides
MMJJ_RS01115: MMJJ_RS01115 - hypothetical protein, at 217,014 to 217,340
_RS01115
MMJJ_RS01120: MMJJ_RS01120 - hypothetical protein, at 217,540 to 217,821
_RS01120
MMJJ_RS01125: MMJJ_RS01125 - hypothetical protein, at 217,831 to 218,205
_RS01125
MMJJ_RS09255: MMJJ_RS09255 - hypothetical protein, at 218,376 to 218,561
_RS09255
MMJJ_RS01135: MMJJ_RS01135 - hypothetical protein, at 218,626 to 222,324
_RS01135
Position (kb) 217
218
219 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 216.866 kb on + strand at 216.960 kb on - strand at 216.960 kb on - strand at 216.961 kb on + strand at 216.962 kb on - strand at 217.121 kb on - strand, within MMJJ_RS01115 at 217.213 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.216 kb on + strand, within MMJJ_RS01115 at 217.270 kb on - strand, within MMJJ_RS01115 at 217.281 kb on - strand, within MMJJ_RS01115 at 217.281 kb on - strand, within MMJJ_RS01115 at 217.282 kb on + strand, within MMJJ_RS01115 at 217.282 kb on + strand, within MMJJ_RS01115 at 217.283 kb on - strand, within MMJJ_RS01115 at 217.283 kb on - strand, within MMJJ_RS01115 at 217.335 kb on + strand at 217.469 kb on + strand at 217.469 kb on + strand at 217.469 kb on + strand at 217.472 kb on + strand at 217.472 kb on + strand at 217.474 kb on - strand at 217.480 kb on - strand at 217.480 kb on - strand at 217.480 kb on - strand at 217.481 kb on + strand at 217.481 kb on + strand at 217.482 kb on - strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.578 kb on - strand, within MMJJ_RS01120 at 217.579 kb on + strand, within MMJJ_RS01120 at 217.580 kb on - strand, within MMJJ_RS01120 at 217.581 kb on + strand, within MMJJ_RS01120 at 217.599 kb on - strand, within MMJJ_RS01120 at 217.600 kb on + strand, within MMJJ_RS01120 at 217.607 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.616 kb on - strand, within MMJJ_RS01120 at 217.617 kb on + strand, within MMJJ_RS01120 at 217.617 kb on + strand, within MMJJ_RS01120 at 217.617 kb on + strand, within MMJJ_RS01120 at 217.624 kb on - strand, within MMJJ_RS01120 at 217.624 kb on - strand, within MMJJ_RS01120 at 217.624 kb on - strand, within MMJJ_RS01120 at 217.625 kb on + strand at 217.625 kb on + strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.626 kb on - strand, within MMJJ_RS01120 at 217.627 kb on + strand, within MMJJ_RS01120 at 217.627 kb on + strand, within MMJJ_RS01120 at 217.628 kb on - strand, within MMJJ_RS01120 at 217.628 kb on - strand, within MMJJ_RS01120 at 217.667 kb on + strand, within MMJJ_RS01120 at 217.686 kb on - strand, within MMJJ_RS01120 at 217.686 kb on - strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.687 kb on + strand, within MMJJ_RS01120 at 217.693 kb on - strand, within MMJJ_RS01120 at 217.694 kb on + strand, within MMJJ_RS01120 at 217.712 kb on + strand, within MMJJ_RS01120 at 217.712 kb on + strand, within MMJJ_RS01120 at 217.722 kb on + strand, within MMJJ_RS01120 at 217.733 kb on + strand, within MMJJ_RS01120 at 217.733 kb on + strand, within MMJJ_RS01120 at 217.774 kb on - strand, within MMJJ_RS01120 at 217.775 kb on + strand, within MMJJ_RS01120 at 217.780 kb on + strand, within MMJJ_RS01120 at 217.819 kb on - strand at 217.820 kb on + strand at 217.851 kb on - strand at 217.851 kb on - strand at 217.851 kb on - strand at 217.852 kb on + strand at 217.855 kb on + strand at 218.067 kb on + strand, within MMJJ_RS01125 at 218.089 kb on - strand, within MMJJ_RS01125 at 218.091 kb on - strand, within MMJJ_RS01125 at 218.094 kb on - strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.095 kb on + strand, within MMJJ_RS01125 at 218.096 kb on - strand, within MMJJ_RS01125 at 218.144 kb on - strand, within MMJJ_RS01125 at 218.216 kb on + strand at 218.216 kb on + strand at 218.241 kb on - strand at 218.242 kb on + strand at 218.242 kb on + strand at 218.244 kb on - strand at 218.250 kb on - strand at 218.258 kb on - strand at 218.314 kb on - strand at 218.315 kb on + strand at 218.377 kb on - strand at 218.379 kb on - strand at 218.380 kb on + strand at 218.412 kb on + strand, within MMJJ_RS09255 at 218.414 kb on - strand, within MMJJ_RS09255 at 218.414 kb on - strand, within MMJJ_RS09255 at 218.442 kb on - strand, within MMJJ_RS09255 at 218.502 kb on + strand, within MMJJ_RS09255 at 218.634 kb on - strand at 218.653 kb on - strand at 218.654 kb on + strand at 218.659 kb on + strand at 218.675 kb on + strand at 218.677 kb on + strand at 218.677 kb on + strand at 218.682 kb on - strand at 218.731 kb on + strand at 218.914 kb on + strand at 218.917 kb on - strand at 219.034 kb on - strand, within MMJJ_RS01135 at 219.109 kb on + strand, within MMJJ_RS01135 at 219.121 kb on + strand, within MMJJ_RS01135
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 216,866 + +0.0 216,960 - +1.0 216,960 - +2.5 216,961 + -0.3 216,962 - -0.8 217,121 - MMJJ_RS01115 0.33 +3.8 217,213 - MMJJ_RS01115 0.61 +0.4 217,215 - MMJJ_RS01115 0.61 +2.3 217,215 - MMJJ_RS01115 0.61 +0.8 217,215 - MMJJ_RS01115 0.61 +0.8 217,216 + MMJJ_RS01115 0.62 -3.4 217,270 - MMJJ_RS01115 0.78 -0.1 217,281 - MMJJ_RS01115 0.82 -3.3 217,281 - MMJJ_RS01115 0.82 -0.2 217,282 + MMJJ_RS01115 0.82 +0.2 217,282 + MMJJ_RS01115 0.82 -1.1 217,283 - MMJJ_RS01115 0.82 -2.4 217,283 - MMJJ_RS01115 0.82 +1.0 217,335 + +0.9 217,469 + -1.4 217,469 + +2.8 217,469 + -0.8 217,472 + +1.1 217,472 + +1.6 217,474 - -0.6 217,480 - -1.2 217,480 - -3.1 217,480 - -0.2 217,481 + -2.6 217,481 + -0.4 217,482 - +0.4 217,483 + +2.5 217,483 + +0.7 217,483 + -0.6 217,483 + -1.2 217,483 + +3.0 217,483 + -0.7 217,483 + -0.1 217,483 + -1.0 217,484 - -2.1 217,484 - -1.8 217,484 - -1.9 217,484 - -3.2 217,484 - -1.3 217,485 + -1.6 217,485 + -0.5 217,485 + -1.0 217,485 + +1.4 217,485 + -2.0 217,486 - -0.6 217,486 - +0.6 217,486 - -1.3 217,486 - -1.0 217,486 - -0.6 217,487 + -0.3 217,487 + +1.9 217,487 + +1.3 217,487 + -0.7 217,578 - MMJJ_RS01120 0.13 +3.4 217,579 + MMJJ_RS01120 0.14 +0.7 217,580 - MMJJ_RS01120 0.14 -0.9 217,581 + MMJJ_RS01120 0.15 +0.7 217,599 - MMJJ_RS01120 0.21 +2.0 217,600 + MMJJ_RS01120 0.21 +1.0 217,607 - MMJJ_RS01120 0.24 -3.2 217,616 - MMJJ_RS01120 0.27 +1.8 217,616 - MMJJ_RS01120 0.27 -0.3 217,616 - MMJJ_RS01120 0.27 -1.4 217,616 - MMJJ_RS01120 0.27 +0.0 217,616 - MMJJ_RS01120 0.27 -1.8 217,617 + MMJJ_RS01120 0.27 +2.2 217,617 + MMJJ_RS01120 0.27 -0.5 217,617 + MMJJ_RS01120 0.27 -0.8 217,624 - MMJJ_RS01120 0.30 -3.0 217,624 - MMJJ_RS01120 0.30 +1.7 217,624 - MMJJ_RS01120 0.30 +1.2 217,625 + +0.0 217,625 + MMJJ_RS01120 0.30 -0.5 217,626 - MMJJ_RS01120 0.30 -2.5 217,626 - MMJJ_RS01120 0.30 -2.4 217,626 - MMJJ_RS01120 0.30 +1.1 217,626 - MMJJ_RS01120 0.30 -2.0 217,626 - MMJJ_RS01120 0.30 -2.7 217,627 + MMJJ_RS01120 0.31 -0.8 217,627 + MMJJ_RS01120 0.31 -0.4 217,628 - MMJJ_RS01120 0.31 -0.2 217,628 - MMJJ_RS01120 0.31 -1.8 217,667 + MMJJ_RS01120 0.45 -1.3 217,686 - MMJJ_RS01120 0.52 +0.9 217,686 - MMJJ_RS01120 0.52 -2.0 217,687 + MMJJ_RS01120 0.52 -0.4 217,687 + MMJJ_RS01120 0.52 +0.7 217,687 + MMJJ_RS01120 0.52 +0.3 217,687 + MMJJ_RS01120 0.52 +1.2 217,693 - MMJJ_RS01120 0.54 +0.4 217,694 + MMJJ_RS01120 0.55 +1.9 217,712 + MMJJ_RS01120 0.61 -0.6 217,712 + MMJJ_RS01120 0.61 +0.1 217,722 + MMJJ_RS01120 0.65 +1.2 217,733 + MMJJ_RS01120 0.68 -0.3 217,733 + MMJJ_RS01120 0.68 -4.4 217,774 - MMJJ_RS01120 0.83 -0.6 217,775 + MMJJ_RS01120 0.83 -2.4 217,780 + MMJJ_RS01120 0.85 -0.6 217,819 - -2.6 217,820 + +0.4 217,851 - +0.6 217,851 - +1.0 217,851 - -2.3 217,852 + -1.3 217,855 + -1.6 218,067 + MMJJ_RS01125 0.63 +1.5 218,089 - MMJJ_RS01125 0.69 -0.4 218,091 - MMJJ_RS01125 0.69 +3.5 218,094 - MMJJ_RS01125 0.70 -1.0 218,095 + MMJJ_RS01125 0.70 +1.6 218,095 + MMJJ_RS01125 0.70 +1.1 218,095 + MMJJ_RS01125 0.70 +2.0 218,096 - MMJJ_RS01125 0.71 +2.6 218,144 - MMJJ_RS01125 0.83 +3.0 218,216 + -1.5 218,216 + -2.0 218,241 - +0.4 218,242 + -1.3 218,242 + +0.6 218,244 - +0.9 218,250 - +5.2 218,258 - +4.4 218,314 - -0.5 218,315 + -3.1 218,377 - +1.0 218,379 - +0.4 218,380 + +1.8 218,412 + MMJJ_RS09255 0.19 -0.9 218,414 - MMJJ_RS09255 0.20 -1.6 218,414 - MMJJ_RS09255 0.20 -1.9 218,442 - MMJJ_RS09255 0.35 +1.6 218,502 + MMJJ_RS09255 0.68 +1.9 218,634 - -0.4 218,653 - -2.2 218,654 + -1.0 218,659 + +0.6 218,675 + +2.2 218,677 + +2.5 218,677 + -0.6 218,682 - -3.3 218,731 + -0.2 218,914 + +0.6 218,917 - -1.3 219,034 - MMJJ_RS01135 0.11 -0.6 219,109 + MMJJ_RS01135 0.13 +1.1 219,121 + MMJJ_RS01135 0.13 -1.6
Or see this region's nucleotide sequence