Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01100

Experiment: McCas_formate_complete Antimycin A 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01095 and MMJJ_RS01100 are separated by 189 nucleotidesMMJJ_RS01100 and MMJJ_RS01105 overlap by 8 nucleotides MMJJ_RS01095: MMJJ_RS01095 - site-specific integrase, at 211,605 to 212,603 _RS01095 MMJJ_RS01100: MMJJ_RS01100 - helix-turn-helix domain-containing protein, at 212,793 to 213,035 _RS01100 MMJJ_RS01105: MMJJ_RS01105 - DUF5906 domain-containing protein, at 213,028 to 215,544 _RS01105 Position (kb) 212 213 214Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 211.853 kb on - strand, within MMJJ_RS01095at 211.854 kb on + strand, within MMJJ_RS01095at 211.893 kb on + strand, within MMJJ_RS01095at 211.943 kb on - strand, within MMJJ_RS01095at 211.944 kb on + strand, within MMJJ_RS01095at 211.944 kb on + strand, within MMJJ_RS01095at 212.095 kb on - strand, within MMJJ_RS01095at 212.145 kb on + strand, within MMJJ_RS01095at 212.151 kb on + strand, within MMJJ_RS01095at 212.158 kb on - strand, within MMJJ_RS01095at 212.159 kb on + strand, within MMJJ_RS01095at 212.159 kb on + strand, within MMJJ_RS01095at 212.237 kb on - strand, within MMJJ_RS01095at 212.367 kb on - strand, within MMJJ_RS01095at 212.368 kb on + strand, within MMJJ_RS01095at 212.440 kb on - strand, within MMJJ_RS01095at 212.440 kb on - strand, within MMJJ_RS01095at 212.467 kb on - strand, within MMJJ_RS01095at 212.467 kb on - strand, within MMJJ_RS01095at 212.468 kb on + strand, within MMJJ_RS01095at 212.468 kb on + strand, within MMJJ_RS01095at 212.485 kb on - strand, within MMJJ_RS01095at 212.485 kb on - strand, within MMJJ_RS01095at 212.532 kb on + strandat 212.539 kb on + strandat 212.558 kb on - strandat 212.629 kb on - strandat 212.629 kb on - strandat 212.668 kb on - strandat 212.669 kb on + strandat 212.672 kb on - strandat 212.715 kb on + strandat 212.716 kb on - strandat 212.717 kb on + strandat 212.717 kb on + strandat 212.717 kb on + strandat 212.718 kb on - strandat 212.718 kb on - strandat 212.719 kb on + strandat 212.719 kb on + strandat 212.720 kb on - strandat 212.720 kb on - strandat 212.753 kb on - strandat 212.777 kb on + strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.814 kb on - strandat 212.816 kb on - strandat 212.817 kb on + strandat 212.822 kb on + strand, within MMJJ_RS01100at 212.853 kb on - strand, within MMJJ_RS01100at 213.025 kb on - strandat 213.025 kb on - strandat 213.026 kb on + strandat 213.026 kb on + strandat 213.026 kb on + strandat 213.034 kb on + strandat 213.038 kb on - strandat 213.038 kb on - strandat 213.038 kb on - strandat 213.039 kb on + strandat 213.039 kb on + strandat 213.081 kb on + strandat 213.090 kb on - strandat 213.098 kb on - strandat 213.170 kb on + strandat 213.264 kb on - strandat 213.283 kb on - strand, within MMJJ_RS01105at 213.283 kb on - strand, within MMJJ_RS01105at 213.288 kb on - strand, within MMJJ_RS01105at 213.289 kb on + strand, within MMJJ_RS01105at 213.339 kb on - strand, within MMJJ_RS01105at 213.340 kb on + strand, within MMJJ_RS01105at 213.340 kb on + strand, within MMJJ_RS01105at 213.344 kb on + strand, within MMJJ_RS01105at 213.352 kb on - strand, within MMJJ_RS01105at 213.352 kb on - strand, within MMJJ_RS01105at 213.358 kb on - strand, within MMJJ_RS01105at 213.424 kb on + strand, within MMJJ_RS01105at 213.479 kb on - strand, within MMJJ_RS01105at 213.541 kb on + strand, within MMJJ_RS01105at 213.604 kb on + strand, within MMJJ_RS01105at 213.604 kb on + strand, within MMJJ_RS01105at 213.645 kb on - strand, within MMJJ_RS01105at 213.684 kb on + strand, within MMJJ_RS01105at 213.780 kb on - strand, within MMJJ_RS01105at 213.916 kb on - strand, within MMJJ_RS01105at 213.952 kb on - strand, within MMJJ_RS01105at 214.026 kb on + strand, within MMJJ_RS01105at 214.030 kb on - strand, within MMJJ_RS01105

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM
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211,853 - MMJJ_RS01095 0.25 -2.3
211,854 + MMJJ_RS01095 0.25 +1.8
211,893 + MMJJ_RS01095 0.29 +2.4
211,943 - MMJJ_RS01095 0.34 -1.8
211,944 + MMJJ_RS01095 0.34 +0.8
211,944 + MMJJ_RS01095 0.34 +2.1
212,095 - MMJJ_RS01095 0.49 +3.1
212,145 + MMJJ_RS01095 0.54 -2.4
212,151 + MMJJ_RS01095 0.55 -2.3
212,158 - MMJJ_RS01095 0.55 +3.5
212,159 + MMJJ_RS01095 0.55 -2.1
212,159 + MMJJ_RS01095 0.55 -3.8
212,237 - MMJJ_RS01095 0.63 -0.1
212,367 - MMJJ_RS01095 0.76 +1.2
212,368 + MMJJ_RS01095 0.76 +1.1
212,440 - MMJJ_RS01095 0.84 -1.1
212,440 - MMJJ_RS01095 0.84 +0.9
212,467 - MMJJ_RS01095 0.86 +0.6
212,467 - MMJJ_RS01095 0.86 -0.0
212,468 + MMJJ_RS01095 0.86 +3.0
212,468 + MMJJ_RS01095 0.86 -2.4
212,485 - MMJJ_RS01095 0.88 -2.3
212,485 - MMJJ_RS01095 0.88 +1.7
212,532 + -0.5
212,539 + -1.7
212,558 - -1.3
212,629 - -0.9
212,629 - -0.7
212,668 - +0.4
212,669 + -0.2
212,672 - +0.6
212,715 + -2.3
212,716 - +1.1
212,717 + +0.0
212,717 + -0.5
212,717 + +1.2
212,718 - +1.6
212,718 - +0.5
212,719 + +1.3
212,719 + +2.5
212,720 - +0.9
212,720 - -1.3
212,753 - -1.3
212,777 + -4.5
212,814 - -1.3
212,814 - +0.9
212,814 - +0.0
212,814 - -0.3
212,814 - -2.0
212,814 - +4.7
212,816 - -0.0
212,817 + +1.0
212,822 + MMJJ_RS01100 0.12 -2.3
212,853 - MMJJ_RS01100 0.25 +2.9
213,025 - +0.0
213,025 - +0.9
213,026 + -1.8
213,026 + -1.8
213,026 + -3.0
213,034 + -1.5
213,038 - +0.9
213,038 - -1.6
213,038 - +0.1
213,039 + +1.4
213,039 + +0.9
213,081 + +2.1
213,090 - -2.3
213,098 - -2.1
213,170 + +2.5
213,264 - +3.0
213,283 - MMJJ_RS01105 0.10 -1.5
213,283 - MMJJ_RS01105 0.10 -3.2
213,288 - MMJJ_RS01105 0.10 -1.6
213,289 + MMJJ_RS01105 0.10 -1.5
213,339 - MMJJ_RS01105 0.12 +0.0
213,340 + MMJJ_RS01105 0.12 +2.0
213,340 + MMJJ_RS01105 0.12 -2.5
213,344 + MMJJ_RS01105 0.13 -2.5
213,352 - MMJJ_RS01105 0.13 +0.0
213,352 - MMJJ_RS01105 0.13 +1.2
213,358 - MMJJ_RS01105 0.13 -0.2
213,424 + MMJJ_RS01105 0.16 +3.0
213,479 - MMJJ_RS01105 0.18 +2.6
213,541 + MMJJ_RS01105 0.20 +3.9
213,604 + MMJJ_RS01105 0.23 -2.4
213,604 + MMJJ_RS01105 0.23 -0.5
213,645 - MMJJ_RS01105 0.25 -3.0
213,684 + MMJJ_RS01105 0.26 -2.0
213,780 - MMJJ_RS01105 0.30 -0.6
213,916 - MMJJ_RS01105 0.35 -2.2
213,952 - MMJJ_RS01105 0.37 -1.6
214,026 + MMJJ_RS01105 0.40 -0.4
214,030 - MMJJ_RS01105 0.40 +1.8

Or see this region's nucleotide sequence