Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00565 and MMJJ_RS00570 are separated by 272 nucleotides MMJJ_RS00570 and MMJJ_RS00575 are separated by 88 nucleotides MMJJ_RS00575 and MMJJ_RS00580 are separated by 22 nucleotides
MMJJ_RS00565: MMJJ_RS00565 - serine--tRNA ligase, at 105,104 to 106,648
_RS00565
MMJJ_RS00570: MMJJ_RS00570 - ribonuclease P, at 106,921 to 107,313
_RS00570
MMJJ_RS00575: MMJJ_RS00575 - carboxylating nicotinate-nucleotide diphosphorylase, at 107,402 to 108,241
_RS00575
MMJJ_RS00580: MMJJ_RS00580 - 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit CofG, at 108,264 to 109,322
_RS00580
Position (kb)
106
107
108 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 106.647 kb on + strand at 106.661 kb on - strand at 106.661 kb on - strand at 106.661 kb on - strand at 106.662 kb on + strand at 106.664 kb on - strand at 106.686 kb on - strand at 106.697 kb on - strand at 106.739 kb on + strand at 106.744 kb on + strand at 106.745 kb on - strand at 106.745 kb on - strand at 106.750 kb on - strand at 106.751 kb on + strand at 106.751 kb on + strand at 106.762 kb on - strand at 106.762 kb on - strand at 106.762 kb on - strand at 106.762 kb on - strand at 106.766 kb on - strand at 106.766 kb on - strand at 106.784 kb on - strand at 106.784 kb on - strand at 106.784 kb on - strand at 106.784 kb on - strand at 106.785 kb on + strand at 106.785 kb on + strand at 106.802 kb on - strand at 106.803 kb on + strand at 106.803 kb on + strand at 106.803 kb on + strand at 106.803 kb on + strand at 106.810 kb on + strand at 106.814 kb on + strand at 106.834 kb on - strand at 106.837 kb on - strand at 106.837 kb on - strand at 106.846 kb on + strand at 106.900 kb on - strand at 106.905 kb on - strand at 106.905 kb on - strand at 106.906 kb on + strand at 106.922 kb on - strand at 106.923 kb on + strand at 107.034 kb on - strand, within MMJJ_RS00570 at 107.035 kb on + strand, within MMJJ_RS00570 at 107.038 kb on - strand, within MMJJ_RS00570 at 107.316 kb on - strand at 107.316 kb on - strand at 107.329 kb on - strand at 107.329 kb on - strand at 107.330 kb on + strand at 107.330 kb on + strand at 107.331 kb on - strand at 107.331 kb on - strand at 107.332 kb on + strand at 107.332 kb on + strand at 107.337 kb on + strand at 107.347 kb on - strand at 107.359 kb on - strand at 107.359 kb on - strand at 107.359 kb on - strand at 107.368 kb on - strand at 107.369 kb on + strand at 107.369 kb on + strand at 107.382 kb on + strand at 107.383 kb on - strand at 107.454 kb on - strand at 108.022 kb on + strand at 108.054 kb on - strand, within MMJJ_RS00575 at 108.066 kb on - strand, within MMJJ_RS00575 at 108.142 kb on - strand, within MMJJ_RS00575 at 108.179 kb on + strand at 108.239 kb on - strand at 108.264 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 106,647 + +0.1 106,661 - +0.8 106,661 - -1.9 106,661 - -1.3 106,662 + +0.9 106,664 - -2.6 106,686 - +1.1 106,697 - +3.4 106,739 + -0.5 106,744 + +3.6 106,745 - -1.1 106,745 - -1.3 106,750 - -1.4 106,751 + +2.0 106,751 + -1.3 106,762 - +0.1 106,762 - -2.7 106,762 - -2.1 106,762 - +1.9 106,766 - -0.5 106,766 - +2.1 106,784 - +0.7 106,784 - -2.1 106,784 - -2.5 106,784 - +2.9 106,785 + -0.8 106,785 + +0.7 106,802 - -0.9 106,803 + +1.6 106,803 + -3.3 106,803 + +2.9 106,803 + +1.4 106,810 + +3.7 106,814 + +0.6 106,834 - -1.4 106,837 - -1.5 106,837 - -1.8 106,846 + +1.4 106,900 - +0.8 106,905 - +2.6 106,905 - +2.3 106,906 + -0.5 106,922 - -0.2 106,923 + +2.0 107,034 - MMJJ_RS00570 0.29 -0.5 107,035 + MMJJ_RS00570 0.29 -1.7 107,038 - MMJJ_RS00570 0.30 +0.1 107,316 - +0.1 107,316 - -0.5 107,329 - +1.1 107,329 - -1.3 107,330 + +1.1 107,330 + -0.9 107,331 - -2.3 107,331 - +0.8 107,332 + -0.9 107,332 + -2.1 107,337 + -2.2 107,347 - +0.2 107,359 - -2.6 107,359 - -1.8 107,359 - -0.6 107,368 - -0.6 107,369 + +3.1 107,369 + +0.1 107,382 + +1.8 107,383 - -2.6 107,454 - -1.7 108,022 + +1.1 108,054 - MMJJ_RS00575 0.78 +0.1 108,066 - MMJJ_RS00575 0.79 -1.7 108,142 - MMJJ_RS00575 0.88 +1.4 108,179 + -1.3 108,239 - -1.9 108,264 + -0.5
Or see this region's nucleotide sequence