Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00035 and MMJJ_RS00040 are separated by 9 nucleotides MMJJ_RS00040 and MMJJ_RS00045 are separated by 11 nucleotides MMJJ_RS00045 and MMJJ_RS00050 are separated by 10 nucleotides MMJJ_RS00050 and MMJJ_RS00055 are separated by 14 nucleotides
MMJJ_RS00035: MMJJ_RS00035 - CO dehydrogenase/acetyl-CoA synthase complex subunit alpha, at 6,409 to 8,751
_RS00035
MMJJ_RS00040: MMJJ_RS00040 - CO dehydrogenase/acetyl-CoA synthase complex subunit epsilon, at 8,761 to 9,279
_RS00040
MMJJ_RS00045: MMJJ_RS00045 - CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta, at 9,291 to 10,664
_RS00045
MMJJ_RS00050: MMJJ_RS00050 - AAA family ATPase, at 10,675 to 11,427
_RS00050
MMJJ_RS00055: MMJJ_RS00055 - CO dehydrogenase/acetyl-CoA synthase subunit delta, at 11,442 to 12,596
_RS00055
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 8.335 kb on + strand, within MMJJ_RS00035 at 8.380 kb on - strand, within MMJJ_RS00035 at 8.380 kb on - strand, within MMJJ_RS00035 at 8.426 kb on - strand, within MMJJ_RS00035 at 8.507 kb on - strand, within MMJJ_RS00035 at 8.507 kb on - strand, within MMJJ_RS00035 at 8.508 kb on + strand, within MMJJ_RS00035 at 8.528 kb on - strand at 8.547 kb on - strand at 8.562 kb on + strand at 8.579 kb on - strand at 8.579 kb on - strand at 8.579 kb on - strand at 8.580 kb on + strand at 8.585 kb on - strand at 8.596 kb on - strand at 8.622 kb on + strand at 8.627 kb on - strand at 8.628 kb on + strand at 8.749 kb on - strand at 8.772 kb on - strand at 8.773 kb on + strand at 8.799 kb on + strand at 8.799 kb on + strand at 8.829 kb on + strand, within MMJJ_RS00040 at 8.897 kb on - strand, within MMJJ_RS00040 at 8.954 kb on - strand, within MMJJ_RS00040 at 8.955 kb on + strand, within MMJJ_RS00040 at 9.038 kb on - strand, within MMJJ_RS00040 at 9.039 kb on + strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.119 kb on + strand, within MMJJ_RS00040 at 9.119 kb on + strand, within MMJJ_RS00040 at 9.121 kb on - strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.194 kb on + strand, within MMJJ_RS00040 at 9.194 kb on + strand, within MMJJ_RS00040 at 9.228 kb on - strand at 9.228 kb on - strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.253 kb on - strand at 9.304 kb on - strand at 9.305 kb on + strand at 9.316 kb on - strand at 9.317 kb on + strand at 9.327 kb on - strand at 9.327 kb on - strand at 9.328 kb on + strand at 9.407 kb on + strand at 9.422 kb on + strand at 9.545 kb on + strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.591 kb on + strand, within MMJJ_RS00045 at 9.598 kb on - strand, within MMJJ_RS00045 at 9.599 kb on + strand, within MMJJ_RS00045 at 9.599 kb on + strand, within MMJJ_RS00045 at 9.661 kb on - strand, within MMJJ_RS00045 at 9.736 kb on - strand, within MMJJ_RS00045 at 9.736 kb on - strand, within MMJJ_RS00045 at 9.757 kb on - strand, within MMJJ_RS00045 at 9.807 kb on + strand, within MMJJ_RS00045 at 9.842 kb on + strand, within MMJJ_RS00045 at 9.846 kb on - strand, within MMJJ_RS00045 at 9.847 kb on + strand, within MMJJ_RS00045 at 9.855 kb on + strand, within MMJJ_RS00045 at 9.892 kb on - strand, within MMJJ_RS00045 at 9.944 kb on - strand, within MMJJ_RS00045 at 10.027 kb on + strand, within MMJJ_RS00045 at 10.031 kb on - strand, within MMJJ_RS00045 at 10.031 kb on - strand, within MMJJ_RS00045 at 10.032 kb on + strand, within MMJJ_RS00045 at 10.055 kb on - strand, within MMJJ_RS00045 at 10.067 kb on - strand, within MMJJ_RS00045 at 10.068 kb on + strand, within MMJJ_RS00045 at 10.082 kb on + strand, within MMJJ_RS00045 at 10.084 kb on + strand, within MMJJ_RS00045 at 10.087 kb on - strand, within MMJJ_RS00045 at 10.088 kb on + strand, within MMJJ_RS00045 at 10.138 kb on - strand, within MMJJ_RS00045 at 10.146 kb on - strand, within MMJJ_RS00045 at 10.199 kb on - strand, within MMJJ_RS00045 at 10.200 kb on + strand, within MMJJ_RS00045 at 10.281 kb on + strand, within MMJJ_RS00045 at 10.281 kb on + strand, within MMJJ_RS00045 at 10.287 kb on - strand, within MMJJ_RS00045 at 10.287 kb on - strand, within MMJJ_RS00045 at 10.288 kb on + strand, within MMJJ_RS00045 at 10.288 kb on + strand, within MMJJ_RS00045 at 10.288 kb on + strand, within MMJJ_RS00045 at 10.289 kb on - strand, within MMJJ_RS00045 at 10.290 kb on + strand, within MMJJ_RS00045 at 10.358 kb on + strand, within MMJJ_RS00045 at 10.365 kb on + strand, within MMJJ_RS00045 at 10.393 kb on - strand, within MMJJ_RS00045 at 10.393 kb on - strand, within MMJJ_RS00045 at 10.393 kb on - strand, within MMJJ_RS00045 at 10.611 kb on + strand at 10.612 kb on - strand at 10.613 kb on + strand at 10.652 kb on + strand at 10.652 kb on + strand at 10.731 kb on + strand at 10.775 kb on - strand, within MMJJ_RS00050 at 10.775 kb on - strand, within MMJJ_RS00050 at 10.776 kb on + strand, within MMJJ_RS00050 at 10.869 kb on - strand, within MMJJ_RS00050 at 10.882 kb on + strand, within MMJJ_RS00050 at 10.882 kb on + strand, within MMJJ_RS00050 at 10.914 kb on + strand, within MMJJ_RS00050 at 10.914 kb on + strand, within MMJJ_RS00050 at 10.940 kb on - strand, within MMJJ_RS00050 at 10.997 kb on + strand, within MMJJ_RS00050 at 11.050 kb on - strand, within MMJJ_RS00050 at 11.060 kb on - strand, within MMJJ_RS00050 at 11.061 kb on + strand, within MMJJ_RS00050 at 11.126 kb on - strand, within MMJJ_RS00050 at 11.127 kb on + strand, within MMJJ_RS00050 at 11.127 kb on + strand, within MMJJ_RS00050 at 11.133 kb on + strand, within MMJJ_RS00050 at 11.228 kb on - strand, within MMJJ_RS00050 at 11.235 kb on - strand, within MMJJ_RS00050 at 11.235 kb on - strand, within MMJJ_RS00050 at 11.299 kb on + strand, within MMJJ_RS00050 at 11.310 kb on + strand, within MMJJ_RS00050 at 11.335 kb on - strand, within MMJJ_RS00050 at 11.497 kb on - strand at 11.518 kb on - strand at 11.519 kb on + strand at 11.533 kb on - strand at 11.540 kb on + strand at 11.540 kb on + strand at 11.546 kb on - strand at 11.552 kb on + strand at 11.629 kb on + strand, within MMJJ_RS00055
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 8,335 + MMJJ_RS00035 0.82 -0.9 8,380 - MMJJ_RS00035 0.84 -3.7 8,380 - MMJJ_RS00035 0.84 +0.1 8,426 - MMJJ_RS00035 0.86 -2.3 8,507 - MMJJ_RS00035 0.90 -1.5 8,507 - MMJJ_RS00035 0.90 -2.9 8,508 + MMJJ_RS00035 0.90 -1.8 8,528 - -1.8 8,547 - +1.1 8,562 + -3.1 8,579 - -2.9 8,579 - -4.2 8,579 - -2.8 8,580 + -1.3 8,585 - -3.6 8,596 - -1.7 8,622 + -3.5 8,627 - -3.2 8,628 + -2.7 8,749 - -0.9 8,772 - -1.9 8,773 + -2.1 8,799 + -1.1 8,799 + -1.3 8,829 + MMJJ_RS00040 0.13 -2.1 8,897 - MMJJ_RS00040 0.26 -3.9 8,954 - MMJJ_RS00040 0.37 -1.7 8,955 + MMJJ_RS00040 0.37 -1.9 9,038 - MMJJ_RS00040 0.53 -2.3 9,039 + MMJJ_RS00040 0.54 -3.1 9,118 - MMJJ_RS00040 0.69 -2.6 9,118 - MMJJ_RS00040 0.69 -4.0 9,118 - MMJJ_RS00040 0.69 -4.3 9,119 + MMJJ_RS00040 0.69 -1.3 9,119 + MMJJ_RS00040 0.69 +1.5 9,121 - MMJJ_RS00040 0.69 -1.4 9,122 + MMJJ_RS00040 0.70 +1.1 9,122 + MMJJ_RS00040 0.70 -2.5 9,122 + MMJJ_RS00040 0.70 -1.9 9,194 + MMJJ_RS00040 0.83 -3.0 9,194 + MMJJ_RS00040 0.83 -0.5 9,228 - -3.8 9,228 - -2.2 9,229 + -0.7 9,229 + -4.6 9,229 + -2.8 9,229 + -1.5 9,229 + -2.7 9,253 - -3.5 9,304 - -0.9 9,305 + -0.6 9,316 - -1.3 9,317 + -2.3 9,327 - -2.5 9,327 - -2.6 9,328 + -2.6 9,407 + -3.0 9,422 + -2.5 9,545 + MMJJ_RS00045 0.18 -3.0 9,576 - MMJJ_RS00045 0.21 -1.5 9,576 - MMJJ_RS00045 0.21 -2.4 9,576 - MMJJ_RS00045 0.21 -1.8 9,591 + MMJJ_RS00045 0.22 -1.7 9,598 - MMJJ_RS00045 0.22 -0.5 9,599 + MMJJ_RS00045 0.22 -1.1 9,599 + MMJJ_RS00045 0.22 -0.7 9,661 - MMJJ_RS00045 0.27 -2.6 9,736 - MMJJ_RS00045 0.32 -0.9 9,736 - MMJJ_RS00045 0.32 -2.9 9,757 - MMJJ_RS00045 0.34 -1.9 9,807 + MMJJ_RS00045 0.38 -1.5 9,842 + MMJJ_RS00045 0.40 +0.1 9,846 - MMJJ_RS00045 0.40 -1.5 9,847 + MMJJ_RS00045 0.40 +0.5 9,855 + MMJJ_RS00045 0.41 -1.9 9,892 - MMJJ_RS00045 0.44 -1.9 9,944 - MMJJ_RS00045 0.48 -2.4 10,027 + MMJJ_RS00045 0.54 -3.7 10,031 - MMJJ_RS00045 0.54 -2.0 10,031 - MMJJ_RS00045 0.54 -1.9 10,032 + MMJJ_RS00045 0.54 -2.1 10,055 - MMJJ_RS00045 0.56 -3.1 10,067 - MMJJ_RS00045 0.56 -1.1 10,068 + MMJJ_RS00045 0.57 -3.6 10,082 + MMJJ_RS00045 0.58 +0.1 10,084 + MMJJ_RS00045 0.58 -2.6 10,087 - MMJJ_RS00045 0.58 -2.6 10,088 + MMJJ_RS00045 0.58 -0.5 10,138 - MMJJ_RS00045 0.62 -2.1 10,146 - MMJJ_RS00045 0.62 -1.7 10,199 - MMJJ_RS00045 0.66 -1.8 10,200 + MMJJ_RS00045 0.66 -1.3 10,281 + MMJJ_RS00045 0.72 -0.5 10,281 + MMJJ_RS00045 0.72 -1.9 10,287 - MMJJ_RS00045 0.72 -4.1 10,287 - MMJJ_RS00045 0.72 +0.1 10,288 + MMJJ_RS00045 0.73 -1.1 10,288 + MMJJ_RS00045 0.73 -3.3 10,288 + MMJJ_RS00045 0.73 -1.6 10,289 - MMJJ_RS00045 0.73 -2.5 10,290 + MMJJ_RS00045 0.73 -1.6 10,358 + MMJJ_RS00045 0.78 +1.1 10,365 + MMJJ_RS00045 0.78 -1.3 10,393 - MMJJ_RS00045 0.80 -0.6 10,393 - MMJJ_RS00045 0.80 -2.2 10,393 - MMJJ_RS00045 0.80 -1.4 10,611 + +0.1 10,612 - +1.1 10,613 + -2.8 10,652 + -1.3 10,652 + -2.5 10,731 + -3.1 10,775 - MMJJ_RS00050 0.13 -3.2 10,775 - MMJJ_RS00050 0.13 -0.5 10,776 + MMJJ_RS00050 0.13 -2.5 10,869 - MMJJ_RS00050 0.26 -3.9 10,882 + MMJJ_RS00050 0.27 -2.7 10,882 + MMJJ_RS00050 0.27 -1.7 10,914 + MMJJ_RS00050 0.32 -1.3 10,914 + MMJJ_RS00050 0.32 -1.9 10,940 - MMJJ_RS00050 0.35 -2.2 10,997 + MMJJ_RS00050 0.43 -2.8 11,050 - MMJJ_RS00050 0.50 -3.2 11,060 - MMJJ_RS00050 0.51 -1.2 11,061 + MMJJ_RS00050 0.51 -2.5 11,126 - MMJJ_RS00050 0.60 -2.5 11,127 + MMJJ_RS00050 0.60 -2.7 11,127 + MMJJ_RS00050 0.60 -1.7 11,133 + MMJJ_RS00050 0.61 -2.8 11,228 - MMJJ_RS00050 0.73 -2.0 11,235 - MMJJ_RS00050 0.74 -0.5 11,235 - MMJJ_RS00050 0.74 -3.9 11,299 + MMJJ_RS00050 0.83 -0.7 11,310 + MMJJ_RS00050 0.84 -3.7 11,335 - MMJJ_RS00050 0.88 -3.7 11,497 - -4.2 11,518 - -3.0 11,519 + -1.7 11,533 - -2.5 11,540 + -1.2 11,540 + -0.5 11,546 - -1.3 11,552 + -2.4 11,629 + MMJJ_RS00055 0.16 -3.2
Or see this region's nucleotide sequence