Experiment: McCas_formate_complete sodium nitrite 0.416666666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07165 and MMJJ_RS07170 are separated by 377 nucleotides MMJJ_RS07170 and MMJJ_RS07175 are separated by 157 nucleotides
MMJJ_RS07165: MMJJ_RS07165 - chromosome segregation protein SMC, at 1,306,768 to 1,310,337
_RS07165
MMJJ_RS07170: MMJJ_RS07170 - pyridoxal phosphate-dependent aminotransferase, at 1,310,715 to 1,312,019
_RS07170
MMJJ_RS07175: MMJJ_RS07175 - DEAD/DEAH box helicase, at 1,312,177 to 1,314,444
_RS07175
Position (kb)
1310
1311
1312
1313 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1309.793 kb on + strand, within MMJJ_RS07165 at 1309.835 kb on + strand, within MMJJ_RS07165 at 1309.864 kb on + strand, within MMJJ_RS07165 at 1309.960 kb on - strand, within MMJJ_RS07165 at 1309.961 kb on + strand, within MMJJ_RS07165 at 1310.007 kb on + strand at 1310.085 kb on - strand at 1310.139 kb on + strand at 1310.139 kb on + strand at 1310.139 kb on + strand at 1310.139 kb on + strand at 1310.142 kb on - strand at 1310.142 kb on - strand at 1310.143 kb on + strand at 1310.145 kb on - strand at 1310.145 kb on - strand at 1310.146 kb on + strand at 1310.207 kb on + strand at 1310.207 kb on + strand at 1310.220 kb on - strand at 1310.221 kb on + strand at 1310.340 kb on - strand at 1310.340 kb on - strand at 1310.341 kb on + strand at 1310.341 kb on + strand at 1310.341 kb on + strand at 1310.375 kb on + strand at 1310.389 kb on + strand at 1310.396 kb on - strand at 1310.397 kb on + strand at 1310.398 kb on - strand at 1310.398 kb on - strand at 1310.398 kb on - strand at 1310.399 kb on + strand at 1310.399 kb on + strand at 1310.410 kb on + strand at 1310.416 kb on - strand at 1310.417 kb on + strand at 1310.418 kb on - strand at 1310.418 kb on - strand at 1310.418 kb on - strand at 1310.419 kb on + strand at 1310.419 kb on + strand at 1310.419 kb on + strand at 1310.419 kb on + strand at 1310.419 kb on + strand at 1310.421 kb on - strand at 1310.421 kb on - strand at 1310.422 kb on + strand at 1310.422 kb on + strand at 1310.422 kb on + strand at 1310.422 kb on + strand at 1310.422 kb on + strand at 1310.479 kb on - strand at 1310.480 kb on + strand at 1310.504 kb on + strand at 1310.528 kb on + strand at 1310.606 kb on - strand at 1310.968 kb on - strand, within MMJJ_RS07170 at 1311.241 kb on - strand, within MMJJ_RS07170 at 1311.352 kb on + strand, within MMJJ_RS07170 at 1311.366 kb on - strand, within MMJJ_RS07170 at 1311.367 kb on + strand, within MMJJ_RS07170 at 1311.367 kb on + strand, within MMJJ_RS07170 at 1311.519 kb on - strand, within MMJJ_RS07170 at 1311.595 kb on - strand, within MMJJ_RS07170 at 1311.618 kb on + strand, within MMJJ_RS07170 at 1311.621 kb on - strand, within MMJJ_RS07170 at 1311.622 kb on + strand, within MMJJ_RS07170 at 1311.622 kb on + strand, within MMJJ_RS07170 at 1311.669 kb on - strand, within MMJJ_RS07170 at 1311.669 kb on - strand, within MMJJ_RS07170 at 1311.704 kb on + strand at 1311.804 kb on - strand, within MMJJ_RS07170 at 1311.852 kb on - strand, within MMJJ_RS07170 at 1311.855 kb on - strand, within MMJJ_RS07170 at 1311.859 kb on - strand, within MMJJ_RS07170 at 1312.005 kb on + strand at 1312.017 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.060 kb on - strand at 1312.061 kb on + strand at 1312.061 kb on + strand at 1312.061 kb on + strand at 1312.067 kb on + strand at 1312.077 kb on - strand at 1312.078 kb on + strand at 1312.084 kb on + strand at 1312.089 kb on - strand at 1312.096 kb on - strand at 1312.096 kb on - strand at 1312.097 kb on + strand at 1312.101 kb on + strand at 1312.103 kb on - strand at 1312.103 kb on - strand at 1312.103 kb on - strand at 1312.104 kb on + strand at 1312.104 kb on + strand at 1312.104 kb on + strand at 1312.104 kb on + strand at 1312.107 kb on - strand at 1312.107 kb on - strand at 1312.108 kb on + strand at 1312.108 kb on + strand at 1312.108 kb on + strand at 1312.109 kb on - strand at 1312.109 kb on - strand at 1312.109 kb on - strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.112 kb on + strand at 1312.112 kb on + strand at 1312.112 kb on + strand at 1312.112 kb on + strand at 1312.116 kb on - strand at 1312.117 kb on + strand at 1312.117 kb on + strand at 1312.117 kb on + strand at 1312.117 kb on + strand at 1312.118 kb on - strand at 1312.118 kb on - strand at 1312.121 kb on - strand at 1312.121 kb on - strand at 1312.122 kb on + strand at 1312.125 kb on + strand at 1312.126 kb on - strand at 1312.126 kb on - strand at 1312.126 kb on - strand at 1312.126 kb on - strand at 1312.127 kb on + strand at 1312.132 kb on - strand at 1312.176 kb on + strand at 1312.184 kb on - strand at 1312.184 kb on - strand at 1312.184 kb on - strand at 1312.184 kb on - strand at 1312.185 kb on + strand at 1312.185 kb on + strand at 1312.245 kb on + strand at 1312.257 kb on + strand at 1312.266 kb on - strand at 1312.266 kb on - strand at 1312.296 kb on - strand at 1312.318 kb on + strand at 1312.507 kb on + strand, within MMJJ_RS07175 at 1312.508 kb on - strand, within MMJJ_RS07175 at 1312.540 kb on + strand, within MMJJ_RS07175 at 1312.545 kb on - strand, within MMJJ_RS07175 at 1312.577 kb on - strand, within MMJJ_RS07175 at 1312.618 kb on - strand, within MMJJ_RS07175 at 1312.695 kb on - strand, within MMJJ_RS07175 at 1312.695 kb on - strand, within MMJJ_RS07175 at 1312.695 kb on - strand, within MMJJ_RS07175 at 1312.696 kb on + strand, within MMJJ_RS07175 at 1312.774 kb on - strand, within MMJJ_RS07175 at 1312.774 kb on - strand, within MMJJ_RS07175 at 1312.775 kb on + strand, within MMJJ_RS07175 at 1312.775 kb on + strand, within MMJJ_RS07175 at 1312.838 kb on - strand, within MMJJ_RS07175 at 1312.905 kb on - strand, within MMJJ_RS07175 at 1312.906 kb on + strand, within MMJJ_RS07175 at 1312.906 kb on + strand, within MMJJ_RS07175 at 1312.906 kb on + strand, within MMJJ_RS07175 at 1312.914 kb on + strand, within MMJJ_RS07175 at 1312.968 kb on - strand, within MMJJ_RS07175 at 1312.969 kb on + strand, within MMJJ_RS07175 at 1312.970 kb on - strand, within MMJJ_RS07175 at 1312.971 kb on + strand, within MMJJ_RS07175 at 1313.012 kb on - strand, within MMJJ_RS07175 at 1313.012 kb on - strand, within MMJJ_RS07175
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sodium nitrite 0.416666666666667 mM remove 1,309,793 + MMJJ_RS07165 0.85 -1.7 1,309,835 + MMJJ_RS07165 0.86 -1.5 1,309,864 + MMJJ_RS07165 0.87 -1.3 1,309,960 - MMJJ_RS07165 0.89 -1.2 1,309,961 + MMJJ_RS07165 0.89 -1.1 1,310,007 + -2.1 1,310,085 - -0.1 1,310,139 + -4.0 1,310,139 + -1.1 1,310,139 + -3.3 1,310,139 + +0.1 1,310,142 - -2.8 1,310,142 - -2.3 1,310,143 + -2.3 1,310,145 - -2.3 1,310,145 - -0.8 1,310,146 + +1.0 1,310,207 + -1.3 1,310,207 + +0.2 1,310,220 - +0.3 1,310,221 + -3.0 1,310,340 - -1.1 1,310,340 - +2.1 1,310,341 + -2.0 1,310,341 + -0.1 1,310,341 + -0.5 1,310,375 + -2.5 1,310,389 + -2.6 1,310,396 - -3.1 1,310,397 + -2.9 1,310,398 - +1.5 1,310,398 - +4.1 1,310,398 - -2.4 1,310,399 + +0.8 1,310,399 + -1.1 1,310,410 + -0.1 1,310,416 - -1.7 1,310,417 + -0.0 1,310,418 - -2.5 1,310,418 - -0.2 1,310,418 - +1.5 1,310,419 + +0.5 1,310,419 + -3.0 1,310,419 + -2.1 1,310,419 + +1.6 1,310,419 + -0.8 1,310,421 - +1.5 1,310,421 - +1.8 1,310,422 + -4.0 1,310,422 + +0.0 1,310,422 + +2.5 1,310,422 + +3.8 1,310,422 + -1.8 1,310,479 - +0.9 1,310,480 + -0.1 1,310,504 + +1.4 1,310,528 + +2.6 1,310,606 - -2.1 1,310,968 - MMJJ_RS07170 0.19 -1.3 1,311,241 - MMJJ_RS07170 0.40 +0.5 1,311,352 + MMJJ_RS07170 0.49 -1.7 1,311,366 - MMJJ_RS07170 0.50 -1.3 1,311,367 + MMJJ_RS07170 0.50 -0.8 1,311,367 + MMJJ_RS07170 0.50 -1.1 1,311,519 - MMJJ_RS07170 0.62 +0.9 1,311,595 - MMJJ_RS07170 0.67 -0.8 1,311,618 + MMJJ_RS07170 0.69 +1.5 1,311,621 - MMJJ_RS07170 0.69 -1.7 1,311,622 + MMJJ_RS07170 0.70 -0.8 1,311,622 + MMJJ_RS07170 0.70 +0.5 1,311,669 - MMJJ_RS07170 0.73 -0.1 1,311,669 - MMJJ_RS07170 0.73 -0.1 1,311,704 + -0.5 1,311,804 - MMJJ_RS07170 0.83 -1.1 1,311,852 - MMJJ_RS07170 0.87 +0.5 1,311,855 - MMJJ_RS07170 0.87 +0.5 1,311,859 - MMJJ_RS07170 0.88 -1.3 1,312,005 + -0.1 1,312,017 - +1.6 1,312,056 - -2.0 1,312,056 - -0.1 1,312,056 - -0.7 1,312,060 - +1.5 1,312,061 + -2.4 1,312,061 + +1.9 1,312,061 + -1.1 1,312,067 + +2.8 1,312,077 - +2.7 1,312,078 + +0.2 1,312,084 + -2.2 1,312,089 - +0.4 1,312,096 - +0.6 1,312,096 - -0.1 1,312,097 + -0.9 1,312,101 + +2.5 1,312,103 - -1.0 1,312,103 - +2.5 1,312,103 - -0.1 1,312,104 + +1.9 1,312,104 + -0.2 1,312,104 + -2.7 1,312,104 + -0.1 1,312,107 - +2.0 1,312,107 - -2.4 1,312,108 + -1.2 1,312,108 + +2.5 1,312,108 + +2.1 1,312,109 - -2.0 1,312,109 - +1.5 1,312,109 - +0.7 1,312,110 + -0.6 1,312,110 + +1.8 1,312,110 + -1.1 1,312,110 + +1.4 1,312,110 + -2.5 1,312,110 + -2.1 1,312,111 - -2.6 1,312,111 - +0.2 1,312,111 - +2.1 1,312,111 - +2.3 1,312,111 - +2.9 1,312,111 - +4.2 1,312,112 + -1.8 1,312,112 + -0.4 1,312,112 + +0.5 1,312,112 + +1.5 1,312,116 - -1.3 1,312,117 + +2.4 1,312,117 + -1.5 1,312,117 + +1.1 1,312,117 + +0.8 1,312,118 - -2.2 1,312,118 - -2.4 1,312,121 - +1.8 1,312,121 - +0.5 1,312,122 + +0.4 1,312,125 + +2.6 1,312,126 - +1.2 1,312,126 - +2.6 1,312,126 - +1.9 1,312,126 - +2.3 1,312,127 + +0.8 1,312,132 - +3.6 1,312,176 + +1.9 1,312,184 - +2.9 1,312,184 - -2.8 1,312,184 - +0.6 1,312,184 - -3.1 1,312,185 + -1.6 1,312,185 + +3.2 1,312,245 + +0.5 1,312,257 + -2.7 1,312,266 - +4.4 1,312,266 - +3.7 1,312,296 - +4.1 1,312,318 + -0.5 1,312,507 + MMJJ_RS07175 0.15 -2.3 1,312,508 - MMJJ_RS07175 0.15 -2.2 1,312,540 + MMJJ_RS07175 0.16 +1.1 1,312,545 - MMJJ_RS07175 0.16 +2.0 1,312,577 - MMJJ_RS07175 0.18 +2.6 1,312,618 - MMJJ_RS07175 0.19 -1.6 1,312,695 - MMJJ_RS07175 0.23 -2.1 1,312,695 - MMJJ_RS07175 0.23 +1.9 1,312,695 - MMJJ_RS07175 0.23 -1.7 1,312,696 + MMJJ_RS07175 0.23 +0.1 1,312,774 - MMJJ_RS07175 0.26 +1.4 1,312,774 - MMJJ_RS07175 0.26 +1.7 1,312,775 + MMJJ_RS07175 0.26 -2.1 1,312,775 + MMJJ_RS07175 0.26 +4.6 1,312,838 - MMJJ_RS07175 0.29 -2.4 1,312,905 - MMJJ_RS07175 0.32 +2.8 1,312,906 + MMJJ_RS07175 0.32 -1.0 1,312,906 + MMJJ_RS07175 0.32 +3.4 1,312,906 + MMJJ_RS07175 0.32 +1.8 1,312,914 + MMJJ_RS07175 0.32 -2.0 1,312,968 - MMJJ_RS07175 0.35 -0.5 1,312,969 + MMJJ_RS07175 0.35 +3.9 1,312,970 - MMJJ_RS07175 0.35 +0.3 1,312,971 + MMJJ_RS07175 0.35 -1.0 1,313,012 - MMJJ_RS07175 0.37 -1.7 1,313,012 - MMJJ_RS07175 0.37 -1.1
Or see this region's nucleotide sequence