Experiment: McCas_formate_complete selenate 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08410 and MMJJ_RS08415 are separated by 172 nucleotides MMJJ_RS08415 and MMJJ_RS08420 overlap by 1 nucleotides MMJJ_RS08420 and MMJJ_RS08425 are separated by 73 nucleotides
MMJJ_RS08410: MMJJ_RS08410 - CBS domain-containing protein, at 1,547,831 to 1,548,193
_RS08410
MMJJ_RS08415: MMJJ_RS08415 - corrinoid protein, at 1,548,366 to 1,549,012
_RS08415
MMJJ_RS08420: MMJJ_RS08420 - uroporphyrinogen decarboxylase family protein, at 1,549,012 to 1,550,070
_RS08420
MMJJ_RS08425: MMJJ_RS08425 - 3-isopropylmalate dehydratase large subunit, at 1,550,144 to 1,551,418
_RS08425
Position (kb)
1549
1550
1551 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1548.070 kb on + strand, within MMJJ_RS08410 at 1548.081 kb on - strand, within MMJJ_RS08410 at 1548.096 kb on - strand, within MMJJ_RS08410 at 1548.211 kb on - strand at 1548.212 kb on + strand at 1548.243 kb on - strand at 1548.247 kb on - strand at 1548.247 kb on - strand at 1548.247 kb on - strand at 1548.247 kb on - strand at 1548.248 kb on + strand at 1548.273 kb on - strand at 1548.276 kb on + strand at 1548.305 kb on - strand at 1548.326 kb on + strand at 1548.328 kb on + strand at 1548.328 kb on + strand at 1548.340 kb on - strand at 1548.340 kb on - strand at 1548.341 kb on + strand at 1548.455 kb on - strand, within MMJJ_RS08415 at 1548.469 kb on - strand, within MMJJ_RS08415 at 1548.497 kb on + strand, within MMJJ_RS08415 at 1548.517 kb on - strand, within MMJJ_RS08415 at 1548.517 kb on - strand, within MMJJ_RS08415 at 1548.517 kb on - strand, within MMJJ_RS08415 at 1548.517 kb on - strand, within MMJJ_RS08415 at 1548.517 kb on - strand, within MMJJ_RS08415 at 1548.517 kb on - strand, within MMJJ_RS08415 at 1548.518 kb on + strand, within MMJJ_RS08415 at 1548.518 kb on + strand, within MMJJ_RS08415 at 1548.518 kb on + strand, within MMJJ_RS08415 at 1548.518 kb on + strand, within MMJJ_RS08415 at 1548.518 kb on + strand, within MMJJ_RS08415 at 1548.543 kb on - strand, within MMJJ_RS08415 at 1548.559 kb on - strand, within MMJJ_RS08415 at 1548.560 kb on + strand, within MMJJ_RS08415 at 1548.582 kb on - strand, within MMJJ_RS08415 at 1548.682 kb on - strand, within MMJJ_RS08415 at 1548.682 kb on - strand, within MMJJ_RS08415 at 1548.683 kb on + strand, within MMJJ_RS08415 at 1548.683 kb on + strand, within MMJJ_RS08415 at 1548.729 kb on - strand, within MMJJ_RS08415 at 1548.729 kb on - strand, within MMJJ_RS08415 at 1548.730 kb on + strand, within MMJJ_RS08415 at 1548.730 kb on + strand, within MMJJ_RS08415 at 1548.730 kb on + strand, within MMJJ_RS08415 at 1548.787 kb on + strand, within MMJJ_RS08415 at 1548.887 kb on - strand, within MMJJ_RS08415 at 1548.913 kb on - strand, within MMJJ_RS08415 at 1548.935 kb on + strand, within MMJJ_RS08415 at 1548.949 kb on - strand at 1548.951 kb on - strand at 1548.951 kb on - strand at 1548.951 kb on - strand at 1548.952 kb on + strand at 1548.953 kb on - strand at 1548.953 kb on - strand at 1548.954 kb on + strand at 1548.954 kb on + strand at 1548.956 kb on + strand at 1548.956 kb on + strand at 1548.956 kb on + strand at 1549.029 kb on - strand at 1549.031 kb on - strand at 1549.041 kb on - strand at 1549.080 kb on + strand at 1549.103 kb on - strand at 1549.104 kb on + strand at 1549.104 kb on + strand at 1549.104 kb on + strand at 1549.104 kb on + strand at 1549.107 kb on - strand at 1549.127 kb on - strand, within MMJJ_RS08420 at 1549.127 kb on - strand, within MMJJ_RS08420 at 1549.128 kb on + strand, within MMJJ_RS08420 at 1549.135 kb on + strand, within MMJJ_RS08420 at 1549.158 kb on + strand, within MMJJ_RS08420 at 1549.222 kb on - strand, within MMJJ_RS08420 at 1549.223 kb on + strand, within MMJJ_RS08420 at 1549.223 kb on + strand, within MMJJ_RS08420 at 1549.226 kb on - strand, within MMJJ_RS08420 at 1549.226 kb on - strand, within MMJJ_RS08420 at 1549.227 kb on + strand, within MMJJ_RS08420 at 1549.243 kb on - strand, within MMJJ_RS08420 at 1549.271 kb on - strand, within MMJJ_RS08420 at 1549.277 kb on + strand, within MMJJ_RS08420 at 1549.332 kb on - strand, within MMJJ_RS08420 at 1549.332 kb on - strand, within MMJJ_RS08420 at 1549.333 kb on + strand, within MMJJ_RS08420 at 1549.360 kb on - strand, within MMJJ_RS08420 at 1549.373 kb on - strand, within MMJJ_RS08420 at 1549.374 kb on + strand, within MMJJ_RS08420 at 1549.374 kb on + strand, within MMJJ_RS08420 at 1549.374 kb on + strand, within MMJJ_RS08420 at 1549.374 kb on + strand, within MMJJ_RS08420 at 1549.379 kb on - strand, within MMJJ_RS08420 at 1549.379 kb on - strand, within MMJJ_RS08420 at 1549.380 kb on + strand, within MMJJ_RS08420 at 1549.380 kb on + strand, within MMJJ_RS08420 at 1549.380 kb on + strand, within MMJJ_RS08420 at 1549.380 kb on + strand, within MMJJ_RS08420 at 1549.380 kb on + strand, within MMJJ_RS08420 at 1549.381 kb on - strand, within MMJJ_RS08420 at 1549.382 kb on + strand, within MMJJ_RS08420 at 1549.382 kb on + strand, within MMJJ_RS08420 at 1549.384 kb on - strand, within MMJJ_RS08420 at 1549.385 kb on + strand, within MMJJ_RS08420 at 1549.385 kb on + strand, within MMJJ_RS08420 at 1549.385 kb on + strand, within MMJJ_RS08420 at 1549.385 kb on + strand, within MMJJ_RS08420 at 1549.415 kb on - strand, within MMJJ_RS08420 at 1549.427 kb on - strand, within MMJJ_RS08420 at 1549.427 kb on - strand, within MMJJ_RS08420 at 1549.496 kb on - strand, within MMJJ_RS08420 at 1549.503 kb on - strand, within MMJJ_RS08420 at 1549.503 kb on - strand, within MMJJ_RS08420 at 1549.503 kb on - strand, within MMJJ_RS08420 at 1549.504 kb on + strand, within MMJJ_RS08420 at 1549.504 kb on + strand, within MMJJ_RS08420 at 1549.507 kb on - strand, within MMJJ_RS08420 at 1549.507 kb on - strand, within MMJJ_RS08420 at 1549.507 kb on - strand, within MMJJ_RS08420 at 1549.508 kb on + strand, within MMJJ_RS08420 at 1549.508 kb on + strand, within MMJJ_RS08420 at 1549.508 kb on + strand, within MMJJ_RS08420 at 1549.508 kb on + strand, within MMJJ_RS08420 at 1549.508 kb on + strand, within MMJJ_RS08420 at 1549.513 kb on + strand, within MMJJ_RS08420 at 1549.524 kb on + strand, within MMJJ_RS08420 at 1549.524 kb on + strand, within MMJJ_RS08420 at 1549.606 kb on + strand, within MMJJ_RS08420 at 1549.634 kb on - strand, within MMJJ_RS08420 at 1549.634 kb on - strand, within MMJJ_RS08420 at 1549.635 kb on + strand, within MMJJ_RS08420 at 1549.638 kb on + strand, within MMJJ_RS08420 at 1549.638 kb on + strand, within MMJJ_RS08420 at 1549.638 kb on + strand, within MMJJ_RS08420 at 1549.638 kb on + strand, within MMJJ_RS08420 at 1549.673 kb on + strand, within MMJJ_RS08420 at 1549.676 kb on - strand, within MMJJ_RS08420 at 1549.676 kb on - strand, within MMJJ_RS08420 at 1549.676 kb on - strand, within MMJJ_RS08420 at 1549.713 kb on - strand, within MMJJ_RS08420 at 1549.713 kb on - strand, within MMJJ_RS08420 at 1549.713 kb on - strand, within MMJJ_RS08420 at 1549.714 kb on + strand, within MMJJ_RS08420 at 1549.714 kb on + strand, within MMJJ_RS08420 at 1549.716 kb on - strand, within MMJJ_RS08420 at 1549.716 kb on - strand, within MMJJ_RS08420 at 1549.717 kb on + strand, within MMJJ_RS08420 at 1549.728 kb on - strand, within MMJJ_RS08420 at 1549.728 kb on - strand, within MMJJ_RS08420 at 1549.728 kb on - strand, within MMJJ_RS08420 at 1549.729 kb on + strand, within MMJJ_RS08420 at 1549.751 kb on - strand, within MMJJ_RS08420 at 1549.752 kb on + strand, within MMJJ_RS08420 at 1549.784 kb on - strand, within MMJJ_RS08420 at 1549.785 kb on + strand, within MMJJ_RS08420 at 1549.785 kb on + strand, within MMJJ_RS08420 at 1549.785 kb on + strand, within MMJJ_RS08420 at 1549.844 kb on - strand, within MMJJ_RS08420 at 1549.859 kb on - strand, within MMJJ_RS08420 at 1549.859 kb on - strand, within MMJJ_RS08420 at 1549.859 kb on - strand, within MMJJ_RS08420 at 1549.859 kb on - strand, within MMJJ_RS08420 at 1549.859 kb on - strand, within MMJJ_RS08420 at 1549.860 kb on + strand, within MMJJ_RS08420 at 1549.860 kb on + strand, within MMJJ_RS08420 at 1549.860 kb on + strand, within MMJJ_RS08420 at 1549.892 kb on - strand, within MMJJ_RS08420 at 1549.915 kb on + strand, within MMJJ_RS08420 at 1550.004 kb on - strand at 1550.033 kb on + strand at 1550.033 kb on + strand at 1550.036 kb on + strand at 1550.072 kb on + strand at 1550.105 kb on - strand at 1550.131 kb on - strand at 1550.132 kb on + strand at 1550.146 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete selenate 31.25 mM remove 1,548,070 + MMJJ_RS08410 0.66 -1.2 1,548,081 - MMJJ_RS08410 0.69 -2.0 1,548,096 - MMJJ_RS08410 0.73 -3.2 1,548,211 - +0.5 1,548,212 + -3.8 1,548,243 - +3.4 1,548,247 - -0.8 1,548,247 - +2.7 1,548,247 - -0.4 1,548,247 - +1.0 1,548,248 + +2.0 1,548,273 - +1.2 1,548,276 + +1.2 1,548,305 - -0.4 1,548,326 + -0.1 1,548,328 + -2.9 1,548,328 + +2.0 1,548,340 - -3.1 1,548,340 - -0.8 1,548,341 + -2.5 1,548,455 - MMJJ_RS08415 0.14 -0.9 1,548,469 - MMJJ_RS08415 0.16 +1.3 1,548,497 + MMJJ_RS08415 0.20 +1.0 1,548,517 - MMJJ_RS08415 0.23 +0.6 1,548,517 - MMJJ_RS08415 0.23 -0.2 1,548,517 - MMJJ_RS08415 0.23 -3.2 1,548,517 - MMJJ_RS08415 0.23 -0.8 1,548,517 - MMJJ_RS08415 0.23 +1.5 1,548,517 - MMJJ_RS08415 0.23 +1.0 1,548,518 + MMJJ_RS08415 0.23 -4.0 1,548,518 + MMJJ_RS08415 0.23 +0.9 1,548,518 + MMJJ_RS08415 0.23 -1.1 1,548,518 + MMJJ_RS08415 0.23 -3.0 1,548,518 + MMJJ_RS08415 0.23 -0.7 1,548,543 - MMJJ_RS08415 0.27 -0.5 1,548,559 - MMJJ_RS08415 0.30 +0.8 1,548,560 + MMJJ_RS08415 0.30 +0.8 1,548,582 - MMJJ_RS08415 0.33 +2.1 1,548,682 - MMJJ_RS08415 0.49 -2.5 1,548,682 - MMJJ_RS08415 0.49 +1.8 1,548,683 + MMJJ_RS08415 0.49 +1.7 1,548,683 + MMJJ_RS08415 0.49 +1.3 1,548,729 - MMJJ_RS08415 0.56 +0.3 1,548,729 - MMJJ_RS08415 0.56 -4.3 1,548,730 + MMJJ_RS08415 0.56 -0.2 1,548,730 + MMJJ_RS08415 0.56 -4.1 1,548,730 + MMJJ_RS08415 0.56 -1.8 1,548,787 + MMJJ_RS08415 0.65 +0.2 1,548,887 - MMJJ_RS08415 0.81 +2.0 1,548,913 - MMJJ_RS08415 0.85 +1.3 1,548,935 + MMJJ_RS08415 0.88 +1.4 1,548,949 - -5.1 1,548,951 - +1.7 1,548,951 - -1.2 1,548,951 - +0.6 1,548,952 + +1.4 1,548,953 - -2.5 1,548,953 - +0.3 1,548,954 + -1.0 1,548,954 + -0.0 1,548,956 + -3.1 1,548,956 + -2.4 1,548,956 + -0.8 1,549,029 - -1.9 1,549,031 - -0.2 1,549,041 - +1.3 1,549,080 + +1.0 1,549,103 - +1.0 1,549,104 + +0.3 1,549,104 + -4.7 1,549,104 + +0.0 1,549,104 + -0.0 1,549,107 - +0.7 1,549,127 - MMJJ_RS08420 0.11 +1.2 1,549,127 - MMJJ_RS08420 0.11 -3.7 1,549,128 + MMJJ_RS08420 0.11 -0.6 1,549,135 + MMJJ_RS08420 0.12 +1.0 1,549,158 + MMJJ_RS08420 0.14 -2.9 1,549,222 - MMJJ_RS08420 0.20 +0.1 1,549,223 + MMJJ_RS08420 0.20 +0.5 1,549,223 + MMJJ_RS08420 0.20 -1.8 1,549,226 - MMJJ_RS08420 0.20 +0.5 1,549,226 - MMJJ_RS08420 0.20 +1.8 1,549,227 + MMJJ_RS08420 0.20 +0.5 1,549,243 - MMJJ_RS08420 0.22 -0.8 1,549,271 - MMJJ_RS08420 0.24 -0.1 1,549,277 + MMJJ_RS08420 0.25 -5.1 1,549,332 - MMJJ_RS08420 0.30 -0.8 1,549,332 - MMJJ_RS08420 0.30 +2.1 1,549,333 + MMJJ_RS08420 0.30 +0.0 1,549,360 - MMJJ_RS08420 0.33 -4.3 1,549,373 - MMJJ_RS08420 0.34 +1.9 1,549,374 + MMJJ_RS08420 0.34 +0.1 1,549,374 + MMJJ_RS08420 0.34 -0.3 1,549,374 + MMJJ_RS08420 0.34 +1.0 1,549,374 + MMJJ_RS08420 0.34 +0.5 1,549,379 - MMJJ_RS08420 0.35 +0.3 1,549,379 - MMJJ_RS08420 0.35 +0.4 1,549,380 + MMJJ_RS08420 0.35 +1.5 1,549,380 + MMJJ_RS08420 0.35 -3.1 1,549,380 + MMJJ_RS08420 0.35 -3.6 1,549,380 + MMJJ_RS08420 0.35 -3.7 1,549,380 + MMJJ_RS08420 0.35 +1.6 1,549,381 - MMJJ_RS08420 0.35 +0.4 1,549,382 + MMJJ_RS08420 0.35 +0.8 1,549,382 + MMJJ_RS08420 0.35 +0.4 1,549,384 - MMJJ_RS08420 0.35 +2.5 1,549,385 + MMJJ_RS08420 0.35 +0.5 1,549,385 + MMJJ_RS08420 0.35 +0.5 1,549,385 + MMJJ_RS08420 0.35 +0.8 1,549,385 + MMJJ_RS08420 0.35 -0.8 1,549,415 - MMJJ_RS08420 0.38 -4.5 1,549,427 - MMJJ_RS08420 0.39 +0.0 1,549,427 - MMJJ_RS08420 0.39 -3.9 1,549,496 - MMJJ_RS08420 0.46 -0.8 1,549,503 - MMJJ_RS08420 0.46 -3.8 1,549,503 - MMJJ_RS08420 0.46 +0.4 1,549,503 - MMJJ_RS08420 0.46 +1.4 1,549,504 + MMJJ_RS08420 0.46 -2.5 1,549,504 + MMJJ_RS08420 0.46 +0.7 1,549,507 - MMJJ_RS08420 0.47 -0.1 1,549,507 - MMJJ_RS08420 0.47 +1.0 1,549,507 - MMJJ_RS08420 0.47 +1.2 1,549,508 + MMJJ_RS08420 0.47 -4.2 1,549,508 + MMJJ_RS08420 0.47 +1.6 1,549,508 + MMJJ_RS08420 0.47 -3.9 1,549,508 + MMJJ_RS08420 0.47 -0.4 1,549,508 + MMJJ_RS08420 0.47 +1.5 1,549,513 + MMJJ_RS08420 0.47 -0.1 1,549,524 + MMJJ_RS08420 0.48 -1.8 1,549,524 + MMJJ_RS08420 0.48 -2.0 1,549,606 + MMJJ_RS08420 0.56 +1.6 1,549,634 - MMJJ_RS08420 0.59 +0.6 1,549,634 - MMJJ_RS08420 0.59 +1.3 1,549,635 + MMJJ_RS08420 0.59 -4.3 1,549,638 + MMJJ_RS08420 0.59 +0.5 1,549,638 + MMJJ_RS08420 0.59 -3.8 1,549,638 + MMJJ_RS08420 0.59 -3.7 1,549,638 + MMJJ_RS08420 0.59 +0.1 1,549,673 + MMJJ_RS08420 0.62 -1.2 1,549,676 - MMJJ_RS08420 0.63 -3.8 1,549,676 - MMJJ_RS08420 0.63 +2.4 1,549,676 - MMJJ_RS08420 0.63 -0.9 1,549,713 - MMJJ_RS08420 0.66 +1.0 1,549,713 - MMJJ_RS08420 0.66 +2.6 1,549,713 - MMJJ_RS08420 0.66 +2.1 1,549,714 + MMJJ_RS08420 0.66 +1.2 1,549,714 + MMJJ_RS08420 0.66 -0.5 1,549,716 - MMJJ_RS08420 0.66 -1.3 1,549,716 - MMJJ_RS08420 0.66 -1.0 1,549,717 + MMJJ_RS08420 0.67 -0.1 1,549,728 - MMJJ_RS08420 0.68 +0.5 1,549,728 - MMJJ_RS08420 0.68 -2.5 1,549,728 - MMJJ_RS08420 0.68 +2.3 1,549,729 + MMJJ_RS08420 0.68 +1.3 1,549,751 - MMJJ_RS08420 0.70 -0.5 1,549,752 + MMJJ_RS08420 0.70 -0.7 1,549,784 - MMJJ_RS08420 0.73 -2.0 1,549,785 + MMJJ_RS08420 0.73 +1.8 1,549,785 + MMJJ_RS08420 0.73 -0.5 1,549,785 + MMJJ_RS08420 0.73 +0.9 1,549,844 - MMJJ_RS08420 0.79 +0.1 1,549,859 - MMJJ_RS08420 0.80 +1.6 1,549,859 - MMJJ_RS08420 0.80 -0.9 1,549,859 - MMJJ_RS08420 0.80 -0.7 1,549,859 - MMJJ_RS08420 0.80 +2.4 1,549,859 - MMJJ_RS08420 0.80 +0.9 1,549,860 + MMJJ_RS08420 0.80 +1.7 1,549,860 + MMJJ_RS08420 0.80 -3.3 1,549,860 + MMJJ_RS08420 0.80 +2.0 1,549,892 - MMJJ_RS08420 0.83 -3.2 1,549,915 + MMJJ_RS08420 0.85 +1.2 1,550,004 - -1.5 1,550,033 + +1.9 1,550,033 + -4.5 1,550,036 + +1.2 1,550,072 + +2.1 1,550,105 - -0.7 1,550,131 - -0.7 1,550,132 + -0.8 1,550,146 + +1.3
Or see this region's nucleotide sequence