Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00865

Experiment: McCas_formate_complete selenate 31.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00860 and MMJJ_RS00865 are separated by 8 nucleotidesMMJJ_RS00865 and MMJJ_RS00870 are separated by 21 nucleotidesMMJJ_RS00870 and MMJJ_RS00875 are separated by 16 nucleotides MMJJ_RS00860: MMJJ_RS00860 - 4Fe-4S binding protein, at 164,474 to 165,667 _RS00860 MMJJ_RS00865: MMJJ_RS00865 - Ni/Fe hydrogenase subunit alpha, at 165,676 to 167,091 _RS00865 MMJJ_RS00870: MMJJ_RS00870 - F420-nonreducing hydrogenase, at 167,113 to 168,015 _RS00870 MMJJ_RS00875: MMJJ_RS00875 - hydrogenase iron-sulfur subunit, at 168,032 to 168,454 _RS00875 Position (kb) 165 166 167 168Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 164.738 kb on + strand, within MMJJ_RS00860at 164.835 kb on + strand, within MMJJ_RS00860at 164.853 kb on - strand, within MMJJ_RS00860at 164.960 kb on - strand, within MMJJ_RS00860at 164.960 kb on - strand, within MMJJ_RS00860at 164.961 kb on + strand, within MMJJ_RS00860at 165.008 kb on - strand, within MMJJ_RS00860at 165.018 kb on + strand, within MMJJ_RS00860at 165.062 kb on - strand, within MMJJ_RS00860at 165.062 kb on - strand, within MMJJ_RS00860at 165.063 kb on + strand, within MMJJ_RS00860at 165.063 kb on + strand, within MMJJ_RS00860at 165.092 kb on + strand, within MMJJ_RS00860at 165.166 kb on - strand, within MMJJ_RS00860at 165.171 kb on - strand, within MMJJ_RS00860at 165.172 kb on + strand, within MMJJ_RS00860at 165.172 kb on + strand, within MMJJ_RS00860at 165.174 kb on + strand, within MMJJ_RS00860at 165.174 kb on + strand, within MMJJ_RS00860at 165.176 kb on + strand, within MMJJ_RS00860at 165.244 kb on - strand, within MMJJ_RS00860at 165.244 kb on - strand, within MMJJ_RS00860at 165.245 kb on + strand, within MMJJ_RS00860at 165.245 kb on + strand, within MMJJ_RS00860at 165.248 kb on - strand, within MMJJ_RS00860at 165.301 kb on - strand, within MMJJ_RS00860at 165.326 kb on - strand, within MMJJ_RS00860at 165.326 kb on - strand, within MMJJ_RS00860at 165.345 kb on - strand, within MMJJ_RS00860at 165.490 kb on - strand, within MMJJ_RS00860at 165.494 kb on + strand, within MMJJ_RS00860at 165.506 kb on - strand, within MMJJ_RS00860at 165.522 kb on + strand, within MMJJ_RS00860at 165.637 kb on - strandat 165.640 kb on + strandat 165.640 kb on + strandat 165.787 kb on - strandat 165.805 kb on - strandat 165.806 kb on + strandat 165.838 kb on - strand, within MMJJ_RS00865at 165.839 kb on + strand, within MMJJ_RS00865at 165.839 kb on + strand, within MMJJ_RS00865at 165.907 kb on - strand, within MMJJ_RS00865at 165.908 kb on + strand, within MMJJ_RS00865at 166.018 kb on - strand, within MMJJ_RS00865at 166.019 kb on + strand, within MMJJ_RS00865at 166.025 kb on + strand, within MMJJ_RS00865at 166.066 kb on - strand, within MMJJ_RS00865at 166.106 kb on - strand, within MMJJ_RS00865at 166.167 kb on + strand, within MMJJ_RS00865at 166.179 kb on - strand, within MMJJ_RS00865at 166.180 kb on + strand, within MMJJ_RS00865at 166.180 kb on + strand, within MMJJ_RS00865at 166.185 kb on - strand, within MMJJ_RS00865at 166.185 kb on - strand, within MMJJ_RS00865at 166.201 kb on - strand, within MMJJ_RS00865at 166.201 kb on - strand, within MMJJ_RS00865at 166.201 kb on - strand, within MMJJ_RS00865at 166.201 kb on - strand, within MMJJ_RS00865at 166.201 kb on - strand, within MMJJ_RS00865at 166.202 kb on + strand, within MMJJ_RS00865at 166.202 kb on + strand, within MMJJ_RS00865at 166.310 kb on - strand, within MMJJ_RS00865at 166.315 kb on - strand, within MMJJ_RS00865at 166.315 kb on - strand, within MMJJ_RS00865at 166.316 kb on + strand, within MMJJ_RS00865at 166.319 kb on - strand, within MMJJ_RS00865at 166.385 kb on - strand, within MMJJ_RS00865at 166.386 kb on + strand, within MMJJ_RS00865at 166.389 kb on - strand, within MMJJ_RS00865at 166.437 kb on + strand, within MMJJ_RS00865at 166.477 kb on - strand, within MMJJ_RS00865at 166.492 kb on - strand, within MMJJ_RS00865at 166.516 kb on - strand, within MMJJ_RS00865at 166.517 kb on + strand, within MMJJ_RS00865at 166.517 kb on + strand, within MMJJ_RS00865at 166.597 kb on - strand, within MMJJ_RS00865at 166.608 kb on - strand, within MMJJ_RS00865at 166.609 kb on + strand, within MMJJ_RS00865at 166.672 kb on - strand, within MMJJ_RS00865at 166.673 kb on + strand, within MMJJ_RS00865at 166.744 kb on - strand, within MMJJ_RS00865at 167.092 kb on - strandat 167.102 kb on - strandat 167.293 kb on + strand, within MMJJ_RS00870at 167.311 kb on + strand, within MMJJ_RS00870at 167.335 kb on - strand, within MMJJ_RS00870at 167.496 kb on - strand, within MMJJ_RS00870at 167.535 kb on - strand, within MMJJ_RS00870at 167.535 kb on - strand, within MMJJ_RS00870at 167.536 kb on + strand, within MMJJ_RS00870at 167.536 kb on + strand, within MMJJ_RS00870at 167.540 kb on + strand, within MMJJ_RS00870at 167.540 kb on + strand, within MMJJ_RS00870at 167.540 kb on + strand, within MMJJ_RS00870at 167.583 kb on - strand, within MMJJ_RS00870at 167.583 kb on - strand, within MMJJ_RS00870at 167.655 kb on - strand, within MMJJ_RS00870at 167.693 kb on - strand, within MMJJ_RS00870at 167.725 kb on - strand, within MMJJ_RS00870at 167.726 kb on + strand, within MMJJ_RS00870at 167.731 kb on + strand, within MMJJ_RS00870at 167.866 kb on + strand, within MMJJ_RS00870at 167.950 kb on - strandat 167.950 kb on - strandat 167.965 kb on - strandat 168.001 kb on + strandat 168.001 kb on + strandat 168.001 kb on + strandat 168.001 kb on + strandat 168.010 kb on - strandat 168.015 kb on - strandat 168.015 kb on - strandat 168.029 kb on - strandat 168.029 kb on - strandat 168.030 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete selenate 31.25 mM
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164,738 + MMJJ_RS00860 0.22 -0.4
164,835 + MMJJ_RS00860 0.30 -0.0
164,853 - MMJJ_RS00860 0.32 -3.4
164,960 - MMJJ_RS00860 0.41 +0.4
164,960 - MMJJ_RS00860 0.41 -3.3
164,961 + MMJJ_RS00860 0.41 +0.4
165,008 - MMJJ_RS00860 0.45 +3.1
165,018 + MMJJ_RS00860 0.46 -3.8
165,062 - MMJJ_RS00860 0.49 -2.2
165,062 - MMJJ_RS00860 0.49 +1.0
165,063 + MMJJ_RS00860 0.49 +2.3
165,063 + MMJJ_RS00860 0.49 +0.0
165,092 + MMJJ_RS00860 0.52 +1.1
165,166 - MMJJ_RS00860 0.58 +0.5
165,171 - MMJJ_RS00860 0.58 -2.7
165,172 + MMJJ_RS00860 0.58 -0.7
165,172 + MMJJ_RS00860 0.58 +1.6
165,174 + MMJJ_RS00860 0.59 +0.1
165,174 + MMJJ_RS00860 0.59 -0.4
165,176 + MMJJ_RS00860 0.59 -0.2
165,244 - MMJJ_RS00860 0.64 -2.9
165,244 - MMJJ_RS00860 0.64 -2.6
165,245 + MMJJ_RS00860 0.65 +0.9
165,245 + MMJJ_RS00860 0.65 +0.4
165,248 - MMJJ_RS00860 0.65 +1.1
165,301 - MMJJ_RS00860 0.69 -1.0
165,326 - MMJJ_RS00860 0.71 -3.4
165,326 - MMJJ_RS00860 0.71 +1.4
165,345 - MMJJ_RS00860 0.73 +0.6
165,490 - MMJJ_RS00860 0.85 -4.5
165,494 + MMJJ_RS00860 0.85 +1.2
165,506 - MMJJ_RS00860 0.86 -0.0
165,522 + MMJJ_RS00860 0.88 +1.0
165,637 - +0.9
165,640 + +0.5
165,640 + +1.6
165,787 - +0.9
165,805 - +0.3
165,806 + -3.4
165,838 - MMJJ_RS00865 0.11 -0.4
165,839 + MMJJ_RS00865 0.12 +1.3
165,839 + MMJJ_RS00865 0.12 +2.5
165,907 - MMJJ_RS00865 0.16 -0.6
165,908 + MMJJ_RS00865 0.16 +0.1
166,018 - MMJJ_RS00865 0.24 -0.6
166,019 + MMJJ_RS00865 0.24 -4.7
166,025 + MMJJ_RS00865 0.25 +0.5
166,066 - MMJJ_RS00865 0.28 -1.0
166,106 - MMJJ_RS00865 0.30 -0.4
166,167 + MMJJ_RS00865 0.35 -0.2
166,179 - MMJJ_RS00865 0.36 -3.6
166,180 + MMJJ_RS00865 0.36 -1.2
166,180 + MMJJ_RS00865 0.36 -2.0
166,185 - MMJJ_RS00865 0.36 -0.8
166,185 - MMJJ_RS00865 0.36 -2.6
166,201 - MMJJ_RS00865 0.37 +1.0
166,201 - MMJJ_RS00865 0.37 +0.3
166,201 - MMJJ_RS00865 0.37 -0.4
166,201 - MMJJ_RS00865 0.37 +0.8
166,201 - MMJJ_RS00865 0.37 -4.6
166,202 + MMJJ_RS00865 0.37 -1.4
166,202 + MMJJ_RS00865 0.37 +2.8
166,310 - MMJJ_RS00865 0.45 -0.1
166,315 - MMJJ_RS00865 0.45 -5.0
166,315 - MMJJ_RS00865 0.45 -1.5
166,316 + MMJJ_RS00865 0.45 -0.2
166,319 - MMJJ_RS00865 0.45 +1.0
166,385 - MMJJ_RS00865 0.50 +2.8
166,386 + MMJJ_RS00865 0.50 -1.6
166,389 - MMJJ_RS00865 0.50 +1.3
166,437 + MMJJ_RS00865 0.54 +0.7
166,477 - MMJJ_RS00865 0.57 -0.6
166,492 - MMJJ_RS00865 0.58 +0.0
166,516 - MMJJ_RS00865 0.59 -2.1
166,517 + MMJJ_RS00865 0.59 -4.2
166,517 + MMJJ_RS00865 0.59 -0.3
166,597 - MMJJ_RS00865 0.65 +1.5
166,608 - MMJJ_RS00865 0.66 +1.0
166,609 + MMJJ_RS00865 0.66 +0.6
166,672 - MMJJ_RS00865 0.70 +0.8
166,673 + MMJJ_RS00865 0.70 -2.1
166,744 - MMJJ_RS00865 0.75 +2.1
167,092 - -3.5
167,102 - -0.7
167,293 + MMJJ_RS00870 0.20 -3.4
167,311 + MMJJ_RS00870 0.22 -3.5
167,335 - MMJJ_RS00870 0.25 +1.1
167,496 - MMJJ_RS00870 0.42 -4.3
167,535 - MMJJ_RS00870 0.47 -0.8
167,535 - MMJJ_RS00870 0.47 -1.0
167,536 + MMJJ_RS00870 0.47 +0.2
167,536 + MMJJ_RS00870 0.47 +0.7
167,540 + MMJJ_RS00870 0.47 +1.0
167,540 + MMJJ_RS00870 0.47 -0.3
167,540 + MMJJ_RS00870 0.47 +0.8
167,583 - MMJJ_RS00870 0.52 +1.9
167,583 - MMJJ_RS00870 0.52 +1.3
167,655 - MMJJ_RS00870 0.60 +1.4
167,693 - MMJJ_RS00870 0.64 -0.2
167,725 - MMJJ_RS00870 0.68 -3.3
167,726 + MMJJ_RS00870 0.68 -3.9
167,731 + MMJJ_RS00870 0.68 -3.2
167,866 + MMJJ_RS00870 0.83 -0.3
167,950 - -2.9
167,950 - -4.4
167,965 - -0.1
168,001 + -1.4
168,001 + +2.0
168,001 + -1.4
168,001 + +0.8
168,010 - -0.1
168,015 - -0.1
168,015 - -1.0
168,029 - -0.7
168,029 - +1.2
168,030 + -0.3

Or see this region's nucleotide sequence