Experiment: McCas_formate_complete Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04270 and MMJJ_RS04275 are separated by 29 nucleotides MMJJ_RS04275 and MMJJ_RS04280 overlap by 1 nucleotides MMJJ_RS04280 and MMJJ_RS04285 are separated by 64 nucleotides
MMJJ_RS04270: MMJJ_RS04270 - DUF364 domain-containing protein, at 795,214 to 795,990
_RS04270
MMJJ_RS04275: MMJJ_RS04275 - ABC transporter ATP-binding protein, at 796,020 to 796,802
_RS04275
MMJJ_RS04280: MMJJ_RS04280 - iron ABC transporter permease, at 796,802 to 797,680
_RS04280
MMJJ_RS04285: MMJJ_RS04285 - ABC transporter substrate-binding protein, at 797,745 to 798,806
_RS04285
Position (kb) 796
797
798 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 795.900 kb on + strand, within MMJJ_RS04270 at 795.936 kb on - strand at 795.993 kb on - strand at 796.005 kb on + strand at 796.015 kb on + strand at 796.036 kb on + strand at 796.074 kb on - strand at 796.074 kb on - strand at 796.075 kb on + strand at 796.075 kb on + strand at 796.091 kb on - strand at 796.096 kb on + strand at 796.183 kb on + strand, within MMJJ_RS04275 at 796.399 kb on + strand, within MMJJ_RS04275 at 796.494 kb on + strand, within MMJJ_RS04275 at 796.537 kb on - strand, within MMJJ_RS04275 at 796.597 kb on - strand, within MMJJ_RS04275 at 796.635 kb on + strand, within MMJJ_RS04275 at 796.675 kb on + strand, within MMJJ_RS04275 at 796.710 kb on - strand, within MMJJ_RS04275 at 796.711 kb on + strand, within MMJJ_RS04275 at 796.711 kb on + strand, within MMJJ_RS04275 at 796.712 kb on - strand, within MMJJ_RS04275 at 796.713 kb on + strand, within MMJJ_RS04275 at 796.713 kb on + strand, within MMJJ_RS04275 at 796.713 kb on + strand, within MMJJ_RS04275 at 796.713 kb on + strand, within MMJJ_RS04275 at 796.743 kb on + strand at 796.743 kb on + strand at 796.775 kb on - strand at 796.776 kb on + strand at 796.783 kb on + strand at 796.785 kb on + strand at 796.803 kb on - strand at 796.804 kb on + strand at 796.829 kb on + strand at 796.829 kb on + strand at 796.863 kb on + strand at 796.863 kb on + strand at 796.867 kb on - strand at 796.868 kb on + strand at 797.006 kb on - strand, within MMJJ_RS04280 at 797.007 kb on + strand, within MMJJ_RS04280 at 797.007 kb on + strand, within MMJJ_RS04280 at 797.007 kb on + strand, within MMJJ_RS04280 at 797.007 kb on + strand, within MMJJ_RS04280 at 797.007 kb on + strand, within MMJJ_RS04280 at 797.060 kb on - strand, within MMJJ_RS04280 at 797.061 kb on + strand, within MMJJ_RS04280 at 797.061 kb on + strand, within MMJJ_RS04280 at 797.061 kb on + strand, within MMJJ_RS04280 at 797.084 kb on + strand, within MMJJ_RS04280 at 797.168 kb on - strand, within MMJJ_RS04280 at 797.169 kb on + strand, within MMJJ_RS04280 at 797.194 kb on - strand, within MMJJ_RS04280 at 797.194 kb on - strand, within MMJJ_RS04280 at 797.194 kb on - strand, within MMJJ_RS04280 at 797.194 kb on - strand, within MMJJ_RS04280 at 797.194 kb on - strand, within MMJJ_RS04280 at 797.195 kb on + strand, within MMJJ_RS04280 at 797.195 kb on + strand, within MMJJ_RS04280 at 797.195 kb on + strand, within MMJJ_RS04280 at 797.195 kb on + strand, within MMJJ_RS04280 at 797.195 kb on + strand, within MMJJ_RS04280 at 797.198 kb on - strand, within MMJJ_RS04280 at 797.198 kb on - strand, within MMJJ_RS04280 at 797.198 kb on - strand, within MMJJ_RS04280 at 797.199 kb on + strand, within MMJJ_RS04280 at 797.199 kb on + strand, within MMJJ_RS04280 at 797.199 kb on + strand, within MMJJ_RS04280 at 797.200 kb on - strand, within MMJJ_RS04280 at 797.301 kb on + strand, within MMJJ_RS04280 at 797.404 kb on + strand, within MMJJ_RS04280 at 797.405 kb on - strand, within MMJJ_RS04280 at 797.405 kb on - strand, within MMJJ_RS04280 at 797.479 kb on - strand, within MMJJ_RS04280 at 797.479 kb on - strand, within MMJJ_RS04280 at 797.480 kb on + strand, within MMJJ_RS04280 at 797.480 kb on + strand, within MMJJ_RS04280 at 797.507 kb on + strand, within MMJJ_RS04280 at 797.507 kb on + strand, within MMJJ_RS04280 at 797.518 kb on - strand, within MMJJ_RS04280 at 797.682 kb on - strand at 797.682 kb on - strand at 797.683 kb on + strand at 797.683 kb on + strand at 797.754 kb on + strand at 797.783 kb on - strand at 797.784 kb on + strand at 797.787 kb on - strand at 797.787 kb on - strand at 797.787 kb on - strand at 797.787 kb on - strand at 797.788 kb on + strand at 797.788 kb on + strand at 797.788 kb on + strand at 797.807 kb on + strand at 797.819 kb on - strand at 797.819 kb on - strand at 797.820 kb on + strand at 797.826 kb on - strand at 797.827 kb on + strand at 797.843 kb on - strand at 797.844 kb on + strand at 797.845 kb on - strand at 797.845 kb on - strand at 797.845 kb on - strand at 797.845 kb on - strand at 797.845 kb on - strand at 797.845 kb on - strand at 797.845 kb on - strand at 797.845 kb on - strand at 797.845 kb on - strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.846 kb on + strand at 797.849 kb on - strand at 797.850 kb on + strand at 797.881 kb on + strand, within MMJJ_RS04285 at 797.881 kb on + strand, within MMJJ_RS04285 at 797.991 kb on - strand, within MMJJ_RS04285 at 797.991 kb on - strand, within MMJJ_RS04285 at 797.991 kb on - strand, within MMJJ_RS04285 at 797.994 kb on - strand, within MMJJ_RS04285 at 797.994 kb on - strand, within MMJJ_RS04285 at 797.996 kb on - strand, within MMJJ_RS04285 at 797.996 kb on - strand, within MMJJ_RS04285 at 797.997 kb on + strand, within MMJJ_RS04285 at 798.006 kb on - strand, within MMJJ_RS04285 at 798.011 kb on - strand, within MMJJ_RS04285 at 798.044 kb on - strand, within MMJJ_RS04285 at 798.048 kb on - strand, within MMJJ_RS04285 at 798.109 kb on + strand, within MMJJ_RS04285 at 798.143 kb on - strand at 798.144 kb on + strand, within MMJJ_RS04285 at 798.144 kb on + strand, within MMJJ_RS04285 at 798.144 kb on + strand, within MMJJ_RS04285 at 798.217 kb on + strand, within MMJJ_RS04285 at 798.286 kb on - strand, within MMJJ_RS04285 at 798.287 kb on + strand, within MMJJ_RS04285 at 798.310 kb on - strand, within MMJJ_RS04285 at 798.328 kb on + strand, within MMJJ_RS04285 at 798.328 kb on + strand, within MMJJ_RS04285 at 798.354 kb on + strand, within MMJJ_RS04285 at 798.358 kb on - strand, within MMJJ_RS04285 at 798.359 kb on + strand, within MMJJ_RS04285 at 798.359 kb on + strand, within MMJJ_RS04285 at 798.359 kb on + strand, within MMJJ_RS04285 at 798.362 kb on - strand, within MMJJ_RS04285 at 798.362 kb on - strand, within MMJJ_RS04285 at 798.363 kb on + strand, within MMJJ_RS04285 at 798.363 kb on + strand, within MMJJ_RS04285 at 798.363 kb on + strand, within MMJJ_RS04285 at 798.383 kb on - strand, within MMJJ_RS04285 at 798.479 kb on - strand, within MMJJ_RS04285 at 798.503 kb on - strand, within MMJJ_RS04285 at 798.503 kb on - strand, within MMJJ_RS04285 at 798.504 kb on + strand, within MMJJ_RS04285 at 798.638 kb on - strand, within MMJJ_RS04285 at 798.645 kb on - strand, within MMJJ_RS04285 at 798.645 kb on - strand, within MMJJ_RS04285 at 798.645 kb on - strand, within MMJJ_RS04285 at 798.645 kb on - strand, within MMJJ_RS04285 at 798.646 kb on + strand, within MMJJ_RS04285 at 798.646 kb on + strand, within MMJJ_RS04285 at 798.665 kb on - strand, within MMJJ_RS04285 at 798.671 kb on - strand, within MMJJ_RS04285 at 798.671 kb on - strand, within MMJJ_RS04285
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM remove 795,900 + MMJJ_RS04270 0.88 +2.3 795,936 - +2.3 795,993 - +0.6 796,005 + -0.9 796,015 + -1.2 796,036 + -0.1 796,074 - -2.2 796,074 - +1.0 796,075 + +3.5 796,075 + -1.1 796,091 - +2.7 796,096 + -2.5 796,183 + MMJJ_RS04275 0.21 +2.1 796,399 + MMJJ_RS04275 0.48 -1.4 796,494 + MMJJ_RS04275 0.61 +3.1 796,537 - MMJJ_RS04275 0.66 -1.1 796,597 - MMJJ_RS04275 0.74 -0.6 796,635 + MMJJ_RS04275 0.79 -3.2 796,675 + MMJJ_RS04275 0.84 -2.5 796,710 - MMJJ_RS04275 0.88 -2.9 796,711 + MMJJ_RS04275 0.88 -1.3 796,711 + MMJJ_RS04275 0.88 +0.3 796,712 - MMJJ_RS04275 0.88 -1.7 796,713 + MMJJ_RS04275 0.89 +2.0 796,713 + MMJJ_RS04275 0.89 +0.7 796,713 + MMJJ_RS04275 0.89 +1.7 796,713 + MMJJ_RS04275 0.89 +1.2 796,743 + -3.6 796,743 + +0.4 796,775 - +0.1 796,776 + -1.8 796,783 + +0.1 796,785 + -2.1 796,803 - -0.1 796,804 + -2.0 796,829 + +0.8 796,829 + -2.0 796,863 + -1.8 796,863 + +3.9 796,867 - -1.6 796,868 + +2.2 797,006 - MMJJ_RS04280 0.23 -0.2 797,007 + MMJJ_RS04280 0.23 +0.3 797,007 + MMJJ_RS04280 0.23 +2.0 797,007 + MMJJ_RS04280 0.23 +2.2 797,007 + MMJJ_RS04280 0.23 -1.1 797,007 + MMJJ_RS04280 0.23 -2.4 797,060 - MMJJ_RS04280 0.29 +1.0 797,061 + MMJJ_RS04280 0.29 +2.9 797,061 + MMJJ_RS04280 0.29 +1.4 797,061 + MMJJ_RS04280 0.29 -3.4 797,084 + MMJJ_RS04280 0.32 -1.8 797,168 - MMJJ_RS04280 0.42 +1.8 797,169 + MMJJ_RS04280 0.42 -0.9 797,194 - MMJJ_RS04280 0.45 +0.0 797,194 - MMJJ_RS04280 0.45 -0.1 797,194 - MMJJ_RS04280 0.45 -0.1 797,194 - MMJJ_RS04280 0.45 -1.8 797,194 - MMJJ_RS04280 0.45 +1.6 797,195 + MMJJ_RS04280 0.45 -1.7 797,195 + MMJJ_RS04280 0.45 +1.2 797,195 + MMJJ_RS04280 0.45 +1.0 797,195 + MMJJ_RS04280 0.45 -2.2 797,195 + MMJJ_RS04280 0.45 +0.4 797,198 - MMJJ_RS04280 0.45 -2.5 797,198 - MMJJ_RS04280 0.45 -1.8 797,198 - MMJJ_RS04280 0.45 +0.3 797,199 + MMJJ_RS04280 0.45 +0.9 797,199 + MMJJ_RS04280 0.45 -2.7 797,199 + MMJJ_RS04280 0.45 +0.8 797,200 - MMJJ_RS04280 0.45 -1.4 797,301 + MMJJ_RS04280 0.57 +4.0 797,404 + MMJJ_RS04280 0.68 +3.0 797,405 - MMJJ_RS04280 0.69 +1.2 797,405 - MMJJ_RS04280 0.69 +3.3 797,479 - MMJJ_RS04280 0.77 +0.4 797,479 - MMJJ_RS04280 0.77 +0.9 797,480 + MMJJ_RS04280 0.77 +1.1 797,480 + MMJJ_RS04280 0.77 -0.2 797,507 + MMJJ_RS04280 0.80 +1.9 797,507 + MMJJ_RS04280 0.80 +0.1 797,518 - MMJJ_RS04280 0.81 +1.2 797,682 - +0.9 797,682 - +1.0 797,683 + -3.2 797,683 + -0.3 797,754 + -0.4 797,783 - -2.0 797,784 + -0.6 797,787 - +1.6 797,787 - +3.1 797,787 - -0.7 797,787 - -2.7 797,788 + -1.7 797,788 + -0.2 797,788 + +0.5 797,807 + -4.2 797,819 - -0.8 797,819 - +1.2 797,820 + +1.9 797,826 - +0.7 797,827 + +2.6 797,843 - +1.5 797,844 + -1.8 797,845 - +1.0 797,845 - -1.4 797,845 - +1.5 797,845 - +0.4 797,845 - -3.8 797,845 - +0.1 797,845 - -1.1 797,845 - +0.1 797,845 - -2.1 797,846 + +1.2 797,846 + -2.7 797,846 + +1.0 797,846 + -0.9 797,846 + +0.2 797,846 + +2.4 797,846 + -3.3 797,846 + -0.8 797,846 + +0.6 797,846 + +4.2 797,846 + +0.9 797,846 + +0.4 797,846 + -3.0 797,846 + +0.7 797,846 + -1.0 797,846 + +1.6 797,846 + -0.8 797,846 + +1.9 797,846 + -0.6 797,846 + -2.4 797,846 + -0.8 797,849 - +0.5 797,850 + +0.4 797,881 + MMJJ_RS04285 0.13 +0.5 797,881 + MMJJ_RS04285 0.13 +1.6 797,991 - MMJJ_RS04285 0.23 -0.5 797,991 - MMJJ_RS04285 0.23 +0.8 797,991 - MMJJ_RS04285 0.23 -1.2 797,994 - MMJJ_RS04285 0.23 -1.5 797,994 - MMJJ_RS04285 0.23 -2.0 797,996 - MMJJ_RS04285 0.24 -1.1 797,996 - MMJJ_RS04285 0.24 -2.7 797,997 + MMJJ_RS04285 0.24 -1.4 798,006 - MMJJ_RS04285 0.25 +1.4 798,011 - MMJJ_RS04285 0.25 +1.7 798,044 - MMJJ_RS04285 0.28 +0.3 798,048 - MMJJ_RS04285 0.29 +0.2 798,109 + MMJJ_RS04285 0.34 -3.0 798,143 - -0.8 798,144 + MMJJ_RS04285 0.38 -0.4 798,144 + MMJJ_RS04285 0.38 +2.3 798,144 + MMJJ_RS04285 0.38 +0.3 798,217 + MMJJ_RS04285 0.44 -0.5 798,286 - MMJJ_RS04285 0.51 +0.2 798,287 + MMJJ_RS04285 0.51 +1.3 798,310 - MMJJ_RS04285 0.53 +1.1 798,328 + MMJJ_RS04285 0.55 +0.6 798,328 + MMJJ_RS04285 0.55 +3.6 798,354 + MMJJ_RS04285 0.57 -3.0 798,358 - MMJJ_RS04285 0.58 -1.4 798,359 + MMJJ_RS04285 0.58 -2.5 798,359 + MMJJ_RS04285 0.58 -1.4 798,359 + MMJJ_RS04285 0.58 -0.9 798,362 - MMJJ_RS04285 0.58 -0.2 798,362 - MMJJ_RS04285 0.58 -1.8 798,363 + MMJJ_RS04285 0.58 -2.1 798,363 + MMJJ_RS04285 0.58 +0.7 798,363 + MMJJ_RS04285 0.58 -1.4 798,383 - MMJJ_RS04285 0.60 -2.4 798,479 - MMJJ_RS04285 0.69 +0.2 798,503 - MMJJ_RS04285 0.71 -0.4 798,503 - MMJJ_RS04285 0.71 -2.8 798,504 + MMJJ_RS04285 0.71 -1.1 798,638 - MMJJ_RS04285 0.84 +0.3 798,645 - MMJJ_RS04285 0.85 +1.5 798,645 - MMJJ_RS04285 0.85 -0.2 798,645 - MMJJ_RS04285 0.85 +1.1 798,645 - MMJJ_RS04285 0.85 -1.1 798,646 + MMJJ_RS04285 0.85 -0.3 798,646 + MMJJ_RS04285 0.85 +1.3 798,665 - MMJJ_RS04285 0.87 +0.2 798,671 - MMJJ_RS04285 0.87 +4.2 798,671 - MMJJ_RS04285 0.87 -1.3
Or see this region's nucleotide sequence