Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01930

Experiment: McCas_formate_complete Irgasan 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01920 and MMJJ_RS01925 are separated by 157 nucleotidesMMJJ_RS01925 and MMJJ_RS01930 are separated by 17 nucleotidesMMJJ_RS01930 and MMJJ_RS01935 are separated by 193 nucleotides MMJJ_RS01920: MMJJ_RS01920 - phosphoglycerate mutase, at 367,404 to 368,690 _RS01920 MMJJ_RS01925: MMJJ_RS01925 - fibrillarin-like rRNA/tRNA 2'-O-methyltransferase, at 368,848 to 369,540 _RS01925 MMJJ_RS01930: MMJJ_RS01930 - Pre-mRNA processing ribonucleoprotein, at 369,558 to 370,988 _RS01930 MMJJ_RS01935: MMJJ_RS01935 - O-phosphoseryl-tRNA(Sec) selenium transferase, at 371,182 to 372,492 _RS01935 Position (kb) 369 370 371Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 368.617 kb on + strandat 368.704 kb on + strandat 368.707 kb on - strandat 368.708 kb on + strandat 368.708 kb on + strandat 368.711 kb on + strandat 368.719 kb on - strandat 368.720 kb on + strandat 368.720 kb on + strandat 368.729 kb on + strandat 368.729 kb on + strandat 368.729 kb on + strandat 368.736 kb on - strandat 368.737 kb on + strandat 368.740 kb on + strandat 368.748 kb on - strandat 368.750 kb on - strandat 368.750 kb on - strandat 368.751 kb on + strandat 368.751 kb on + strandat 368.751 kb on + strandat 368.752 kb on - strandat 368.752 kb on - strandat 368.752 kb on - strandat 368.753 kb on + strandat 368.758 kb on - strandat 368.765 kb on + strandat 368.784 kb on - strandat 368.784 kb on - strandat 368.785 kb on + strandat 368.786 kb on - strandat 368.787 kb on + strandat 368.787 kb on + strandat 368.788 kb on - strandat 368.788 kb on - strandat 368.788 kb on - strandat 368.788 kb on - strandat 368.789 kb on + strandat 368.789 kb on + strandat 368.807 kb on - strandat 368.823 kb on + strandat 368.828 kb on - strandat 368.836 kb on - strandat 368.836 kb on - strandat 368.840 kb on + strandat 368.842 kb on + strandat 368.849 kb on - strandat 368.849 kb on - strandat 368.985 kb on - strand, within MMJJ_RS01925at 369.010 kb on + strand, within MMJJ_RS01925at 369.237 kb on + strand, within MMJJ_RS01925at 369.258 kb on - strand, within MMJJ_RS01925at 369.280 kb on + strand, within MMJJ_RS01925at 369.388 kb on - strand, within MMJJ_RS01925at 369.393 kb on - strand, within MMJJ_RS01925at 369.395 kb on - strand, within MMJJ_RS01925at 369.395 kb on - strand, within MMJJ_RS01925at 369.400 kb on - strand, within MMJJ_RS01925at 369.400 kb on - strand, within MMJJ_RS01925at 369.400 kb on - strand, within MMJJ_RS01925at 369.400 kb on - strand, within MMJJ_RS01925at 369.401 kb on + strand, within MMJJ_RS01925at 369.402 kb on - strand, within MMJJ_RS01925at 369.414 kb on + strand, within MMJJ_RS01925at 369.414 kb on + strand, within MMJJ_RS01925at 369.415 kb on - strand, within MMJJ_RS01925at 369.415 kb on - strand, within MMJJ_RS01925at 369.418 kb on - strand, within MMJJ_RS01925at 369.451 kb on - strand, within MMJJ_RS01925at 369.475 kb on + strandat 369.475 kb on + strandat 369.502 kb on - strandat 369.502 kb on - strandat 369.502 kb on - strandat 369.502 kb on - strandat 369.502 kb on - strandat 369.503 kb on + strandat 369.503 kb on + strandat 369.503 kb on + strandat 369.503 kb on + strandat 369.503 kb on + strandat 369.559 kb on - strandat 369.560 kb on + strandat 369.815 kb on - strand, within MMJJ_RS01930at 369.951 kb on + strand, within MMJJ_RS01930at 370.044 kb on - strand, within MMJJ_RS01930at 370.045 kb on + strand, within MMJJ_RS01930at 370.063 kb on + strand, within MMJJ_RS01930at 370.064 kb on - strand, within MMJJ_RS01930at 370.099 kb on + strand, within MMJJ_RS01930at 370.303 kb on + strand, within MMJJ_RS01930at 370.474 kb on - strand, within MMJJ_RS01930at 370.497 kb on + strand, within MMJJ_RS01930at 370.516 kb on - strand, within MMJJ_RS01930at 370.516 kb on - strand, within MMJJ_RS01930at 370.519 kb on + strand, within MMJJ_RS01930at 370.607 kb on - strand, within MMJJ_RS01930at 370.608 kb on + strand, within MMJJ_RS01930at 370.727 kb on + strand, within MMJJ_RS01930at 370.770 kb on + strand, within MMJJ_RS01930at 370.770 kb on + strand, within MMJJ_RS01930at 370.773 kb on + strand, within MMJJ_RS01930at 370.864 kb on + strandat 370.988 kb on - strandat 371.009 kb on + strandat 371.031 kb on - strandat 371.032 kb on + strandat 371.053 kb on - strandat 371.053 kb on - strandat 371.058 kb on + strandat 371.073 kb on - strandat 371.076 kb on - strandat 371.079 kb on - strandat 371.083 kb on - strandat 371.083 kb on - strandat 371.084 kb on + strandat 371.106 kb on + strandat 371.180 kb on - strandat 371.365 kb on + strand, within MMJJ_RS01935at 371.410 kb on - strand, within MMJJ_RS01935at 371.410 kb on - strand, within MMJJ_RS01935at 371.657 kb on - strand, within MMJJ_RS01935at 371.718 kb on - strand, within MMJJ_RS01935at 371.758 kb on - strand, within MMJJ_RS01935

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM
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368,617 + +1.3
368,704 + -0.7
368,707 - -1.0
368,708 + -0.0
368,708 + +0.7
368,711 + -0.6
368,719 - -1.7
368,720 + -0.7
368,720 + -1.7
368,729 + -1.2
368,729 + +3.3
368,729 + +0.8
368,736 - -4.7
368,737 + +0.4
368,740 + -0.5
368,748 - +0.8
368,750 - -0.3
368,750 - -1.6
368,751 + -0.5
368,751 + +2.9
368,751 + +1.3
368,752 - +3.3
368,752 - +4.4
368,752 - -0.7
368,753 + +0.9
368,758 - -0.8
368,765 + -0.6
368,784 - -0.4
368,784 - +0.0
368,785 + +4.7
368,786 - +2.0
368,787 + -3.1
368,787 + -1.5
368,788 - -0.2
368,788 - +1.4
368,788 - +2.3
368,788 - -2.3
368,789 + -0.2
368,789 + +0.5
368,807 - -2.6
368,823 + +0.3
368,828 - -0.2
368,836 - -3.8
368,836 - +1.6
368,840 + +1.1
368,842 + +0.2
368,849 - -0.2
368,849 - +1.8
368,985 - MMJJ_RS01925 0.20 -2.8
369,010 + MMJJ_RS01925 0.23 -2.7
369,237 + MMJJ_RS01925 0.56 -1.1
369,258 - MMJJ_RS01925 0.59 -3.7
369,280 + MMJJ_RS01925 0.62 -2.6
369,388 - MMJJ_RS01925 0.78 -1.3
369,393 - MMJJ_RS01925 0.79 -1.9
369,395 - MMJJ_RS01925 0.79 -1.8
369,395 - MMJJ_RS01925 0.79 -1.2
369,400 - MMJJ_RS01925 0.80 -3.0
369,400 - MMJJ_RS01925 0.80 -2.1
369,400 - MMJJ_RS01925 0.80 -2.4
369,400 - MMJJ_RS01925 0.80 -0.2
369,401 + MMJJ_RS01925 0.80 -2.4
369,402 - MMJJ_RS01925 0.80 -3.0
369,414 + MMJJ_RS01925 0.82 -1.3
369,414 + MMJJ_RS01925 0.82 -0.9
369,415 - MMJJ_RS01925 0.82 -3.0
369,415 - MMJJ_RS01925 0.82 -0.5
369,418 - MMJJ_RS01925 0.82 -1.1
369,451 - MMJJ_RS01925 0.87 -1.4
369,475 + -3.3
369,475 + +1.1
369,502 - +0.4
369,502 - -1.6
369,502 - +2.4
369,502 - -0.1
369,502 - +0.7
369,503 + -1.1
369,503 + -1.7
369,503 + -0.9
369,503 + -1.6
369,503 + -0.9
369,559 - +0.8
369,560 + +1.6
369,815 - MMJJ_RS01930 0.18 +0.6
369,951 + MMJJ_RS01930 0.27 -0.8
370,044 - MMJJ_RS01930 0.34 +0.2
370,045 + MMJJ_RS01930 0.34 -2.1
370,063 + MMJJ_RS01930 0.35 -1.9
370,064 - MMJJ_RS01930 0.35 -1.8
370,099 + MMJJ_RS01930 0.38 -2.4
370,303 + MMJJ_RS01930 0.52 -3.6
370,474 - MMJJ_RS01930 0.64 -2.9
370,497 + MMJJ_RS01930 0.66 -0.1
370,516 - MMJJ_RS01930 0.67 -3.7
370,516 - MMJJ_RS01930 0.67 -0.8
370,519 + MMJJ_RS01930 0.67 -4.1
370,607 - MMJJ_RS01930 0.73 +0.2
370,608 + MMJJ_RS01930 0.73 -2.0
370,727 + MMJJ_RS01930 0.82 -0.9
370,770 + MMJJ_RS01930 0.85 -0.5
370,770 + MMJJ_RS01930 0.85 -2.3
370,773 + MMJJ_RS01930 0.85 -2.5
370,864 + -1.2
370,988 - -3.7
371,009 + +1.1
371,031 - -0.6
371,032 + +1.0
371,053 - -1.9
371,053 - -1.9
371,058 + -0.7
371,073 - -0.4
371,076 - -2.7
371,079 - -2.9
371,083 - -1.2
371,083 - +2.1
371,084 + +1.1
371,106 + +3.2
371,180 - -0.9
371,365 + MMJJ_RS01935 0.14 +1.1
371,410 - MMJJ_RS01935 0.17 +0.1
371,410 - MMJJ_RS01935 0.17 +1.1
371,657 - MMJJ_RS01935 0.36 -1.5
371,718 - MMJJ_RS01935 0.41 +1.1
371,758 - MMJJ_RS01935 0.44 -0.5

Or see this region's nucleotide sequence