Experiment: McCas_formate_complete Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01620 and MMJJ_RS01625 are separated by 119 nucleotides MMJJ_RS01625 and MMJJ_RS01630 are separated by 21 nucleotides
MMJJ_RS01620: MMJJ_RS01620 - ketol-acid reductoisomerase, at 313,620 to 314,612
_RS01620
MMJJ_RS01625: MMJJ_RS01625 - bile acid:sodium symporter family protein, at 314,732 to 315,697
_RS01625
MMJJ_RS01630: MMJJ_RS01630 - hypothetical protein, at 315,719 to 316,171
_RS01630
Position (kb) 314
315
316 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 314.648 kb on - strand at 314.655 kb on + strand at 314.656 kb on - strand at 314.664 kb on - strand at 314.686 kb on + strand at 314.755 kb on + strand at 314.796 kb on + strand at 314.796 kb on + strand at 314.893 kb on - strand, within MMJJ_RS01625 at 314.911 kb on - strand, within MMJJ_RS01625 at 314.920 kb on + strand, within MMJJ_RS01625 at 314.923 kb on - strand, within MMJJ_RS01625 at 314.923 kb on - strand, within MMJJ_RS01625 at 314.946 kb on - strand, within MMJJ_RS01625 at 314.989 kb on - strand, within MMJJ_RS01625 at 315.016 kb on + strand, within MMJJ_RS01625 at 315.022 kb on - strand, within MMJJ_RS01625 at 315.023 kb on + strand, within MMJJ_RS01625 at 315.027 kb on - strand, within MMJJ_RS01625 at 315.036 kb on - strand, within MMJJ_RS01625 at 315.036 kb on - strand, within MMJJ_RS01625 at 315.037 kb on + strand, within MMJJ_RS01625 at 315.066 kb on - strand, within MMJJ_RS01625 at 315.102 kb on - strand, within MMJJ_RS01625 at 315.102 kb on - strand, within MMJJ_RS01625 at 315.103 kb on + strand, within MMJJ_RS01625 at 315.103 kb on + strand, within MMJJ_RS01625 at 315.105 kb on - strand, within MMJJ_RS01625 at 315.106 kb on + strand, within MMJJ_RS01625 at 315.106 kb on + strand, within MMJJ_RS01625 at 315.106 kb on + strand, within MMJJ_RS01625 at 315.106 kb on + strand, within MMJJ_RS01625 at 315.106 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.110 kb on - strand, within MMJJ_RS01625 at 315.111 kb on + strand, within MMJJ_RS01625 at 315.111 kb on + strand, within MMJJ_RS01625 at 315.111 kb on + strand, within MMJJ_RS01625 at 315.182 kb on + strand, within MMJJ_RS01625 at 315.195 kb on - strand, within MMJJ_RS01625 at 315.195 kb on - strand, within MMJJ_RS01625 at 315.198 kb on - strand, within MMJJ_RS01625 at 315.199 kb on + strand, within MMJJ_RS01625 at 315.199 kb on + strand, within MMJJ_RS01625 at 315.219 kb on - strand, within MMJJ_RS01625 at 315.234 kb on + strand, within MMJJ_RS01625 at 315.247 kb on + strand, within MMJJ_RS01625 at 315.264 kb on - strand, within MMJJ_RS01625 at 315.282 kb on - strand, within MMJJ_RS01625 at 315.289 kb on + strand, within MMJJ_RS01625 at 315.293 kb on + strand, within MMJJ_RS01625 at 315.294 kb on - strand, within MMJJ_RS01625 at 315.294 kb on - strand, within MMJJ_RS01625 at 315.294 kb on - strand, within MMJJ_RS01625 at 315.298 kb on - strand, within MMJJ_RS01625 at 315.298 kb on - strand, within MMJJ_RS01625 at 315.340 kb on + strand, within MMJJ_RS01625 at 315.364 kb on + strand, within MMJJ_RS01625 at 315.411 kb on - strand, within MMJJ_RS01625 at 315.412 kb on + strand, within MMJJ_RS01625 at 315.424 kb on - strand, within MMJJ_RS01625 at 315.425 kb on + strand, within MMJJ_RS01625 at 315.432 kb on - strand, within MMJJ_RS01625 at 315.478 kb on - strand, within MMJJ_RS01625 at 315.478 kb on - strand, within MMJJ_RS01625 at 315.478 kb on - strand, within MMJJ_RS01625 at 315.526 kb on - strand, within MMJJ_RS01625 at 315.531 kb on - strand, within MMJJ_RS01625 at 315.531 kb on - strand, within MMJJ_RS01625 at 315.531 kb on - strand, within MMJJ_RS01625 at 315.532 kb on + strand, within MMJJ_RS01625 at 315.564 kb on - strand, within MMJJ_RS01625 at 315.565 kb on + strand, within MMJJ_RS01625 at 315.637 kb on - strand at 315.638 kb on + strand at 315.659 kb on - strand at 315.710 kb on + strand at 315.712 kb on + strand at 315.712 kb on + strand at 315.720 kb on - strand at 315.721 kb on + strand at 315.721 kb on + strand at 315.722 kb on - strand at 315.722 kb on - strand at 315.723 kb on + strand at 315.732 kb on + strand at 315.768 kb on + strand, within MMJJ_RS01630 at 315.768 kb on + strand, within MMJJ_RS01630 at 315.771 kb on - strand, within MMJJ_RS01630 at 315.771 kb on - strand, within MMJJ_RS01630 at 315.788 kb on + strand, within MMJJ_RS01630 at 315.815 kb on - strand, within MMJJ_RS01630 at 315.815 kb on - strand, within MMJJ_RS01630 at 315.831 kb on + strand, within MMJJ_RS01630 at 315.861 kb on + strand, within MMJJ_RS01630 at 315.897 kb on + strand, within MMJJ_RS01630 at 315.927 kb on + strand, within MMJJ_RS01630 at 315.937 kb on - strand, within MMJJ_RS01630 at 315.938 kb on + strand at 315.963 kb on + strand, within MMJJ_RS01630 at 315.963 kb on + strand, within MMJJ_RS01630 at 315.987 kb on - strand, within MMJJ_RS01630 at 315.988 kb on + strand, within MMJJ_RS01630 at 315.988 kb on + strand, within MMJJ_RS01630 at 315.988 kb on + strand, within MMJJ_RS01630 at 315.996 kb on + strand, within MMJJ_RS01630 at 315.996 kb on + strand, within MMJJ_RS01630 at 316.008 kb on - strand, within MMJJ_RS01630 at 316.016 kb on - strand, within MMJJ_RS01630 at 316.016 kb on - strand, within MMJJ_RS01630 at 316.022 kb on + strand, within MMJJ_RS01630 at 316.063 kb on + strand, within MMJJ_RS01630 at 316.063 kb on + strand, within MMJJ_RS01630 at 316.064 kb on - strand, within MMJJ_RS01630 at 316.072 kb on - strand, within MMJJ_RS01630 at 316.072 kb on - strand, within MMJJ_RS01630 at 316.098 kb on + strand, within MMJJ_RS01630 at 316.098 kb on + strand, within MMJJ_RS01630 at 316.103 kb on + strand, within MMJJ_RS01630 at 316.249 kb on - strand at 316.249 kb on - strand at 316.250 kb on + strand at 316.252 kb on + strand at 316.270 kb on - strand at 316.271 kb on + strand at 316.271 kb on + strand at 316.271 kb on + strand at 316.272 kb on - strand at 316.273 kb on + strand at 316.361 kb on + strand at 316.374 kb on - strand at 316.375 kb on + strand at 316.378 kb on + strand at 316.397 kb on + strand at 316.397 kb on + strand at 316.397 kb on + strand at 316.408 kb on - strand at 316.409 kb on + strand at 316.410 kb on - strand at 316.439 kb on - strand at 316.472 kb on - strand at 316.555 kb on - strand at 316.573 kb on - strand at 316.676 kb on + strand at 316.679 kb on - strand at 316.680 kb on + strand at 316.680 kb on + strand at 316.680 kb on + strand at 316.680 kb on + strand at 316.682 kb on - strand at 316.682 kb on - strand at 316.682 kb on - strand at 316.682 kb on - strand at 316.683 kb on + strand at 316.683 kb on + strand at 316.683 kb on + strand at 316.685 kb on + strand at 316.685 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM remove 314,648 - +1.1 314,655 + -1.5 314,656 - +1.1 314,664 - +0.1 314,686 + +4.1 314,755 + -3.9 314,796 + +0.4 314,796 + +1.3 314,893 - MMJJ_RS01625 0.17 +1.5 314,911 - MMJJ_RS01625 0.19 +1.5 314,920 + MMJJ_RS01625 0.19 +2.2 314,923 - MMJJ_RS01625 0.20 -0.9 314,923 - MMJJ_RS01625 0.20 +1.1 314,946 - MMJJ_RS01625 0.22 -1.7 314,989 - MMJJ_RS01625 0.27 -1.9 315,016 + MMJJ_RS01625 0.29 +0.2 315,022 - MMJJ_RS01625 0.30 +3.1 315,023 + MMJJ_RS01625 0.30 -0.5 315,027 - MMJJ_RS01625 0.31 +2.7 315,036 - MMJJ_RS01625 0.31 -1.2 315,036 - MMJJ_RS01625 0.31 +4.7 315,037 + MMJJ_RS01625 0.32 +3.8 315,066 - MMJJ_RS01625 0.35 +1.7 315,102 - MMJJ_RS01625 0.38 -1.7 315,102 - MMJJ_RS01625 0.38 -3.0 315,103 + MMJJ_RS01625 0.38 -0.1 315,103 + MMJJ_RS01625 0.38 +0.1 315,105 - MMJJ_RS01625 0.39 +1.7 315,106 + MMJJ_RS01625 0.39 +0.3 315,106 + MMJJ_RS01625 0.39 +1.0 315,106 + MMJJ_RS01625 0.39 +1.9 315,106 + MMJJ_RS01625 0.39 -1.2 315,106 - MMJJ_RS01625 0.39 +1.4 315,110 - MMJJ_RS01625 0.39 +5.1 315,110 - MMJJ_RS01625 0.39 +1.9 315,110 - MMJJ_RS01625 0.39 +2.8 315,110 - MMJJ_RS01625 0.39 -1.8 315,110 - MMJJ_RS01625 0.39 +0.5 315,110 - MMJJ_RS01625 0.39 +2.7 315,111 + MMJJ_RS01625 0.39 -0.7 315,111 + MMJJ_RS01625 0.39 -1.5 315,111 + MMJJ_RS01625 0.39 +0.2 315,182 + MMJJ_RS01625 0.47 +1.8 315,195 - MMJJ_RS01625 0.48 -1.7 315,195 - MMJJ_RS01625 0.48 -0.5 315,198 - MMJJ_RS01625 0.48 +3.7 315,199 + MMJJ_RS01625 0.48 -0.5 315,199 + MMJJ_RS01625 0.48 -1.5 315,219 - MMJJ_RS01625 0.50 -4.0 315,234 + MMJJ_RS01625 0.52 +2.1 315,247 + MMJJ_RS01625 0.53 -2.3 315,264 - MMJJ_RS01625 0.55 -1.6 315,282 - MMJJ_RS01625 0.57 +0.5 315,289 + MMJJ_RS01625 0.58 +1.6 315,293 + MMJJ_RS01625 0.58 +0.6 315,294 - MMJJ_RS01625 0.58 +1.1 315,294 - MMJJ_RS01625 0.58 +3.2 315,294 - MMJJ_RS01625 0.58 +0.5 315,298 - MMJJ_RS01625 0.59 +0.9 315,298 - MMJJ_RS01625 0.59 -0.4 315,340 + MMJJ_RS01625 0.63 +2.6 315,364 + MMJJ_RS01625 0.65 +0.9 315,411 - MMJJ_RS01625 0.70 +3.5 315,412 + MMJJ_RS01625 0.70 -0.1 315,424 - MMJJ_RS01625 0.72 +0.7 315,425 + MMJJ_RS01625 0.72 -0.4 315,432 - MMJJ_RS01625 0.72 -1.3 315,478 - MMJJ_RS01625 0.77 +4.1 315,478 - MMJJ_RS01625 0.77 -2.7 315,478 - MMJJ_RS01625 0.77 -0.4 315,526 - MMJJ_RS01625 0.82 +2.1 315,531 - MMJJ_RS01625 0.83 +0.6 315,531 - MMJJ_RS01625 0.83 -0.9 315,531 - MMJJ_RS01625 0.83 -2.6 315,532 + MMJJ_RS01625 0.83 +2.5 315,564 - MMJJ_RS01625 0.86 -2.4 315,565 + MMJJ_RS01625 0.86 -0.3 315,637 - +2.6 315,638 + -2.9 315,659 - +3.9 315,710 + -0.3 315,712 + -0.3 315,712 + +0.6 315,720 - -0.7 315,721 + +1.0 315,721 + +3.0 315,722 - -2.4 315,722 - -2.1 315,723 + +0.2 315,732 + -1.7 315,768 + MMJJ_RS01630 0.11 +0.3 315,768 + MMJJ_RS01630 0.11 -3.3 315,771 - MMJJ_RS01630 0.11 +1.9 315,771 - MMJJ_RS01630 0.11 -0.1 315,788 + MMJJ_RS01630 0.15 +0.4 315,815 - MMJJ_RS01630 0.21 -1.0 315,815 - MMJJ_RS01630 0.21 -2.6 315,831 + MMJJ_RS01630 0.25 +0.5 315,861 + MMJJ_RS01630 0.31 -1.8 315,897 + MMJJ_RS01630 0.39 -1.4 315,927 + MMJJ_RS01630 0.46 -0.4 315,937 - MMJJ_RS01630 0.48 -1.5 315,938 + +0.1 315,963 + MMJJ_RS01630 0.54 -0.2 315,963 + MMJJ_RS01630 0.54 +2.1 315,987 - MMJJ_RS01630 0.59 +0.8 315,988 + MMJJ_RS01630 0.59 -1.9 315,988 + MMJJ_RS01630 0.59 -2.1 315,988 + MMJJ_RS01630 0.59 +2.8 315,996 + MMJJ_RS01630 0.61 -1.3 315,996 + MMJJ_RS01630 0.61 -3.7 316,008 - MMJJ_RS01630 0.64 -1.9 316,016 - MMJJ_RS01630 0.66 -1.8 316,016 - MMJJ_RS01630 0.66 +2.2 316,022 + MMJJ_RS01630 0.67 -0.1 316,063 + MMJJ_RS01630 0.76 +1.4 316,063 + MMJJ_RS01630 0.76 -0.9 316,064 - MMJJ_RS01630 0.76 -0.8 316,072 - MMJJ_RS01630 0.78 +1.1 316,072 - MMJJ_RS01630 0.78 -3.0 316,098 + MMJJ_RS01630 0.84 +2.1 316,098 + MMJJ_RS01630 0.84 +0.5 316,103 + MMJJ_RS01630 0.85 +2.8 316,249 - +3.5 316,249 - +0.5 316,250 + -3.0 316,252 + +5.7 316,270 - +0.4 316,271 + -0.9 316,271 + -2.9 316,271 + -3.5 316,272 - -3.5 316,273 + -3.8 316,361 + -0.9 316,374 - -1.5 316,375 + +0.2 316,378 + +0.9 316,397 + +1.9 316,397 + -1.5 316,397 + -1.9 316,408 - +1.2 316,409 + -0.9 316,410 - -0.9 316,439 - -0.5 316,472 - -2.1 316,555 - +2.1 316,573 - -3.2 316,676 + +2.4 316,679 - -1.7 316,680 + +0.1 316,680 + +1.4 316,680 + +1.4 316,680 + +4.3 316,682 - +2.1 316,682 - -1.6 316,682 - -0.2 316,682 - -1.5 316,683 + -3.7 316,683 + -1.3 316,683 + +1.2 316,685 + +0.1 316,685 + +0.6
Or see this region's nucleotide sequence