Experiment: McCas_formate_complete hypophosphite 62.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06425 and MMJJ_RS06430 are separated by 268 nucleotides MMJJ_RS06430 and MMJJ_RS06435 are separated by 60 nucleotides MMJJ_RS06435 and MMJJ_RS06440 are separated by 153 nucleotides MMJJ_RS06440 and MMJJ_RS06445 are separated by 21 nucleotides
MMJJ_RS06425: MMJJ_RS06425 - 50S ribosomal protein L3, at 1,187,866 to 1,188,870
_RS06425
MMJJ_RS06430: MMJJ_RS06430 - haloacid dehalogenase, at 1,189,139 to 1,189,729
_RS06430
MMJJ_RS06435: MMJJ_RS06435 - tRNA-Ile, at 1,189,790 to 1,189,864
_RS06435
MMJJ_RS06440: MMJJ_RS06440 - DUF4870 domain-containing protein, at 1,190,018 to 1,190,335
_RS06440
MMJJ_RS06445: MMJJ_RS06445 - hypothetical protein, at 1,190,357 to 1,191,178
_RS06445
Position (kb)
1189
1190 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1189.011 kb on + strand at 1189.041 kb on - strand at 1189.050 kb on + strand at 1189.065 kb on - strand at 1189.074 kb on + strand at 1189.099 kb on - strand at 1189.104 kb on + strand at 1189.115 kb on - strand at 1189.115 kb on - strand at 1189.116 kb on + strand at 1189.154 kb on - strand at 1189.158 kb on - strand at 1189.240 kb on + strand, within MMJJ_RS06430 at 1189.370 kb on - strand, within MMJJ_RS06430 at 1189.371 kb on + strand, within MMJJ_RS06430 at 1189.371 kb on + strand, within MMJJ_RS06430 at 1189.371 kb on + strand, within MMJJ_RS06430 at 1189.375 kb on + strand, within MMJJ_RS06430 at 1189.375 kb on + strand, within MMJJ_RS06430 at 1189.388 kb on - strand, within MMJJ_RS06430 at 1189.388 kb on - strand, within MMJJ_RS06430 at 1189.391 kb on - strand, within MMJJ_RS06430 at 1189.446 kb on - strand, within MMJJ_RS06430 at 1189.447 kb on + strand, within MMJJ_RS06430 at 1189.458 kb on + strand, within MMJJ_RS06430 at 1189.460 kb on + strand, within MMJJ_RS06430 at 1189.493 kb on + strand, within MMJJ_RS06430 at 1189.493 kb on + strand, within MMJJ_RS06430 at 1189.501 kb on + strand, within MMJJ_RS06430 at 1189.557 kb on + strand, within MMJJ_RS06430 at 1189.558 kb on - strand, within MMJJ_RS06430 at 1189.558 kb on - strand, within MMJJ_RS06430 at 1189.558 kb on - strand, within MMJJ_RS06430 at 1189.559 kb on + strand, within MMJJ_RS06430 at 1189.559 kb on + strand, within MMJJ_RS06430 at 1189.599 kb on + strand, within MMJJ_RS06430 at 1189.618 kb on - strand, within MMJJ_RS06430 at 1189.671 kb on - strand at 1189.671 kb on - strand at 1189.671 kb on - strand at 1189.671 kb on - strand at 1189.671 kb on - strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.675 kb on - strand at 1189.675 kb on - strand at 1189.700 kb on + strand at 1189.700 kb on + strand at 1189.700 kb on + strand at 1189.700 kb on + strand at 1189.704 kb on - strand at 1189.704 kb on - strand at 1189.704 kb on - strand at 1189.704 kb on - strand at 1189.704 kb on - strand at 1189.705 kb on + strand at 1189.705 kb on + strand at 1189.727 kb on - strand at 1189.728 kb on + strand at 1189.728 kb on + strand at 1189.748 kb on + strand at 1189.753 kb on - strand at 1189.780 kb on - strand at 1189.781 kb on + strand at 1189.929 kb on - strand at 1189.930 kb on + strand at 1189.960 kb on - strand at 1189.961 kb on + strand at 1189.982 kb on - strand at 1189.982 kb on - strand at 1189.982 kb on - strand at 1189.982 kb on - strand at 1189.983 kb on + strand at 1189.983 kb on + strand at 1189.983 kb on + strand at 1189.983 kb on + strand at 1189.983 kb on + strand at 1189.983 kb on + strand at 1189.984 kb on - strand at 1189.984 kb on - strand at 1189.984 kb on - strand at 1189.984 kb on - strand at 1189.984 kb on - strand at 1189.984 kb on - strand at 1189.985 kb on + strand at 1189.985 kb on + strand at 1189.985 kb on + strand at 1189.985 kb on + strand at 1189.985 kb on + strand at 1189.985 kb on + strand at 1189.985 kb on + strand at 1189.989 kb on - strand at 1189.989 kb on - strand at 1189.989 kb on - strand at 1189.990 kb on + strand at 1189.990 kb on + strand at 1190.012 kb on - strand at 1190.013 kb on + strand at 1190.025 kb on - strand at 1190.026 kb on + strand at 1190.036 kb on + strand at 1190.064 kb on + strand, within MMJJ_RS06440 at 1190.067 kb on - strand, within MMJJ_RS06440 at 1190.068 kb on + strand, within MMJJ_RS06440 at 1190.068 kb on + strand, within MMJJ_RS06440 at 1190.068 kb on + strand, within MMJJ_RS06440 at 1190.070 kb on - strand, within MMJJ_RS06440 at 1190.070 kb on - strand, within MMJJ_RS06440 at 1190.088 kb on - strand, within MMJJ_RS06440 at 1190.089 kb on + strand, within MMJJ_RS06440 at 1190.094 kb on - strand, within MMJJ_RS06440 at 1190.094 kb on - strand, within MMJJ_RS06440 at 1190.094 kb on - strand, within MMJJ_RS06440 at 1190.094 kb on - strand, within MMJJ_RS06440 at 1190.095 kb on + strand, within MMJJ_RS06440 at 1190.095 kb on + strand, within MMJJ_RS06440 at 1190.117 kb on + strand, within MMJJ_RS06440 at 1190.124 kb on - strand, within MMJJ_RS06440 at 1190.146 kb on - strand, within MMJJ_RS06440 at 1190.147 kb on + strand, within MMJJ_RS06440 at 1190.166 kb on - strand, within MMJJ_RS06440 at 1190.176 kb on + strand, within MMJJ_RS06440 at 1190.194 kb on - strand, within MMJJ_RS06440 at 1190.195 kb on + strand, within MMJJ_RS06440 at 1190.195 kb on + strand, within MMJJ_RS06440 at 1190.260 kb on + strand, within MMJJ_RS06440 at 1190.260 kb on + strand, within MMJJ_RS06440 at 1190.269 kb on + strand, within MMJJ_RS06440 at 1190.292 kb on + strand, within MMJJ_RS06440 at 1190.292 kb on + strand, within MMJJ_RS06440 at 1190.303 kb on + strand, within MMJJ_RS06440 at 1190.350 kb on - strand at 1190.356 kb on + strand at 1190.359 kb on + strand at 1190.360 kb on - strand at 1190.360 kb on - strand at 1190.360 kb on - strand at 1190.360 kb on - strand at 1190.360 kb on - strand at 1190.360 kb on - strand at 1190.361 kb on + strand at 1190.361 kb on + strand at 1190.361 kb on + strand at 1190.453 kb on + strand, within MMJJ_RS06445 at 1190.453 kb on + strand, within MMJJ_RS06445 at 1190.461 kb on - strand, within MMJJ_RS06445 at 1190.461 kb on - strand, within MMJJ_RS06445 at 1190.471 kb on - strand, within MMJJ_RS06445 at 1190.471 kb on - strand, within MMJJ_RS06445 at 1190.472 kb on + strand, within MMJJ_RS06445 at 1190.472 kb on + strand, within MMJJ_RS06445 at 1190.492 kb on + strand, within MMJJ_RS06445 at 1190.518 kb on - strand, within MMJJ_RS06445 at 1190.591 kb on - strand, within MMJJ_RS06445 at 1190.591 kb on - strand, within MMJJ_RS06445 at 1190.591 kb on - strand, within MMJJ_RS06445 at 1190.591 kb on - strand, within MMJJ_RS06445 at 1190.591 kb on - strand, within MMJJ_RS06445 at 1190.591 kb on - strand, within MMJJ_RS06445 at 1190.592 kb on + strand, within MMJJ_RS06445 at 1190.592 kb on + strand, within MMJJ_RS06445 at 1190.592 kb on + strand, within MMJJ_RS06445 at 1190.592 kb on + strand, within MMJJ_RS06445 at 1190.596 kb on - strand, within MMJJ_RS06445 at 1190.597 kb on + strand, within MMJJ_RS06445 at 1190.597 kb on + strand, within MMJJ_RS06445 at 1190.597 kb on + strand, within MMJJ_RS06445 at 1190.609 kb on - strand, within MMJJ_RS06445 at 1190.610 kb on + strand, within MMJJ_RS06445 at 1190.610 kb on + strand, within MMJJ_RS06445 at 1190.615 kb on + strand, within MMJJ_RS06445 at 1190.623 kb on - strand, within MMJJ_RS06445 at 1190.623 kb on - strand, within MMJJ_RS06445 at 1190.623 kb on - strand, within MMJJ_RS06445 at 1190.624 kb on + strand, within MMJJ_RS06445 at 1190.624 kb on + strand, within MMJJ_RS06445 at 1190.624 kb on + strand, within MMJJ_RS06445 at 1190.633 kb on - strand, within MMJJ_RS06445 at 1190.633 kb on - strand, within MMJJ_RS06445 at 1190.634 kb on + strand, within MMJJ_RS06445 at 1190.634 kb on + strand, within MMJJ_RS06445 at 1190.662 kb on - strand, within MMJJ_RS06445 at 1190.662 kb on - strand, within MMJJ_RS06445 at 1190.662 kb on - strand, within MMJJ_RS06445 at 1190.662 kb on - strand, within MMJJ_RS06445 at 1190.662 kb on - strand, within MMJJ_RS06445 at 1190.662 kb on - strand, within MMJJ_RS06445 at 1190.662 kb on - strand, within MMJJ_RS06445 at 1190.663 kb on + strand, within MMJJ_RS06445 at 1190.663 kb on + strand, within MMJJ_RS06445 at 1190.663 kb on + strand, within MMJJ_RS06445 at 1190.663 kb on + strand, within MMJJ_RS06445 at 1190.664 kb on - strand, within MMJJ_RS06445 at 1190.665 kb on + strand, within MMJJ_RS06445 at 1190.665 kb on + strand, within MMJJ_RS06445 at 1190.665 kb on + strand, within MMJJ_RS06445 at 1190.713 kb on - strand, within MMJJ_RS06445 at 1190.723 kb on - strand, within MMJJ_RS06445 at 1190.723 kb on - strand, within MMJJ_RS06445 at 1190.723 kb on - strand, within MMJJ_RS06445 at 1190.723 kb on - strand, within MMJJ_RS06445 at 1190.723 kb on - strand, within MMJJ_RS06445 at 1190.723 kb on - strand, within MMJJ_RS06445 at 1190.723 kb on - strand, within MMJJ_RS06445 at 1190.723 kb on - strand, within MMJJ_RS06445 at 1190.724 kb on + strand, within MMJJ_RS06445 at 1190.724 kb on + strand, within MMJJ_RS06445 at 1190.724 kb on + strand, within MMJJ_RS06445 at 1190.727 kb on - strand, within MMJJ_RS06445 at 1190.727 kb on - strand, within MMJJ_RS06445 at 1190.727 kb on - strand, within MMJJ_RS06445 at 1190.727 kb on - strand, within MMJJ_RS06445 at 1190.727 kb on - strand, within MMJJ_RS06445 at 1190.728 kb on + strand, within MMJJ_RS06445 at 1190.800 kb on - strand, within MMJJ_RS06445 at 1190.829 kb on - strand, within MMJJ_RS06445 at 1190.829 kb on - strand, within MMJJ_RS06445 at 1190.829 kb on - strand, within MMJJ_RS06445 at 1190.829 kb on - strand, within MMJJ_RS06445 at 1190.830 kb on + strand, within MMJJ_RS06445 at 1190.830 kb on + strand, within MMJJ_RS06445 at 1190.830 kb on + strand, within MMJJ_RS06445
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete hypophosphite 62.5 mM remove 1,189,011 + -1.6 1,189,041 - -0.9 1,189,050 + +2.2 1,189,065 - -0.9 1,189,074 + +1.9 1,189,099 - -4.4 1,189,104 + -2.8 1,189,115 - +0.9 1,189,115 - +1.8 1,189,116 + +1.7 1,189,154 - +2.5 1,189,158 - +2.2 1,189,240 + MMJJ_RS06430 0.17 +0.3 1,189,370 - MMJJ_RS06430 0.39 -0.5 1,189,371 + MMJJ_RS06430 0.39 +0.1 1,189,371 + MMJJ_RS06430 0.39 +0.8 1,189,371 + MMJJ_RS06430 0.39 +1.4 1,189,375 + MMJJ_RS06430 0.40 +0.5 1,189,375 + MMJJ_RS06430 0.40 +0.2 1,189,388 - MMJJ_RS06430 0.42 -1.5 1,189,388 - MMJJ_RS06430 0.42 +1.8 1,189,391 - MMJJ_RS06430 0.43 +1.0 1,189,446 - MMJJ_RS06430 0.52 +1.4 1,189,447 + MMJJ_RS06430 0.52 +3.7 1,189,458 + MMJJ_RS06430 0.54 +0.0 1,189,460 + MMJJ_RS06430 0.54 +2.3 1,189,493 + MMJJ_RS06430 0.60 +0.7 1,189,493 + MMJJ_RS06430 0.60 +0.9 1,189,501 + MMJJ_RS06430 0.61 +1.5 1,189,557 + MMJJ_RS06430 0.71 +1.1 1,189,558 - MMJJ_RS06430 0.71 -1.3 1,189,558 - MMJJ_RS06430 0.71 -1.5 1,189,558 - MMJJ_RS06430 0.71 -0.6 1,189,559 + MMJJ_RS06430 0.71 +0.6 1,189,559 + MMJJ_RS06430 0.71 -1.9 1,189,599 + MMJJ_RS06430 0.78 +3.9 1,189,618 - MMJJ_RS06430 0.81 -0.9 1,189,671 - -0.0 1,189,671 - -0.9 1,189,671 - -1.1 1,189,671 - +0.3 1,189,671 - +1.8 1,189,672 + +1.0 1,189,672 + +0.0 1,189,672 + -1.9 1,189,672 + +0.4 1,189,672 + -0.3 1,189,672 + -0.3 1,189,672 + +0.0 1,189,672 + +1.6 1,189,672 + +1.2 1,189,672 + +2.1 1,189,672 + +0.6 1,189,675 - -0.2 1,189,675 - +3.4 1,189,700 + +0.8 1,189,700 + +1.4 1,189,700 + +1.2 1,189,700 + +3.0 1,189,704 - +1.8 1,189,704 - -0.9 1,189,704 - +2.3 1,189,704 - -1.0 1,189,704 - +1.6 1,189,705 + +0.0 1,189,705 + -2.9 1,189,727 - +0.6 1,189,728 + +1.0 1,189,728 + +1.3 1,189,748 + +0.7 1,189,753 - +3.0 1,189,780 - +0.5 1,189,781 + +1.8 1,189,929 - +1.2 1,189,930 + +0.5 1,189,960 - +3.5 1,189,961 + +1.2 1,189,982 - +3.4 1,189,982 - +1.5 1,189,982 - +0.6 1,189,982 - +1.0 1,189,983 + +1.7 1,189,983 + +2.6 1,189,983 + -0.3 1,189,983 + +0.7 1,189,983 + -0.1 1,189,983 + +2.1 1,189,984 - -2.1 1,189,984 - +0.7 1,189,984 - -2.1 1,189,984 - -4.3 1,189,984 - -2.1 1,189,984 - -1.3 1,189,985 + -2.1 1,189,985 + -0.3 1,189,985 + +0.7 1,189,985 + -0.2 1,189,985 + +0.4 1,189,985 + +1.0 1,189,985 + -3.4 1,189,989 - +0.5 1,189,989 - -0.2 1,189,989 - +0.6 1,189,990 + +0.2 1,189,990 + -2.4 1,190,012 - -0.8 1,190,013 + +1.1 1,190,025 - -0.2 1,190,026 + +2.5 1,190,036 + +2.4 1,190,064 + MMJJ_RS06440 0.14 -1.0 1,190,067 - MMJJ_RS06440 0.15 -0.1 1,190,068 + MMJJ_RS06440 0.16 +2.1 1,190,068 + MMJJ_RS06440 0.16 -1.3 1,190,068 + MMJJ_RS06440 0.16 -3.9 1,190,070 - MMJJ_RS06440 0.16 +0.3 1,190,070 - MMJJ_RS06440 0.16 -0.9 1,190,088 - MMJJ_RS06440 0.22 -0.3 1,190,089 + MMJJ_RS06440 0.22 -0.9 1,190,094 - MMJJ_RS06440 0.24 +0.6 1,190,094 - MMJJ_RS06440 0.24 -0.9 1,190,094 - MMJJ_RS06440 0.24 +2.3 1,190,094 - MMJJ_RS06440 0.24 +2.8 1,190,095 + MMJJ_RS06440 0.24 -0.5 1,190,095 + MMJJ_RS06440 0.24 -0.9 1,190,117 + MMJJ_RS06440 0.31 +0.5 1,190,124 - MMJJ_RS06440 0.33 +1.4 1,190,146 - MMJJ_RS06440 0.40 +2.2 1,190,147 + MMJJ_RS06440 0.41 -0.0 1,190,166 - MMJJ_RS06440 0.47 +1.8 1,190,176 + MMJJ_RS06440 0.50 +1.5 1,190,194 - MMJJ_RS06440 0.55 +1.1 1,190,195 + MMJJ_RS06440 0.56 +1.9 1,190,195 + MMJJ_RS06440 0.56 +0.9 1,190,260 + MMJJ_RS06440 0.76 +1.1 1,190,260 + MMJJ_RS06440 0.76 +0.4 1,190,269 + MMJJ_RS06440 0.79 +0.8 1,190,292 + MMJJ_RS06440 0.86 -0.5 1,190,292 + MMJJ_RS06440 0.86 +1.0 1,190,303 + MMJJ_RS06440 0.90 +0.2 1,190,350 - +0.7 1,190,356 + +0.8 1,190,359 + -2.7 1,190,360 - -0.1 1,190,360 - +0.6 1,190,360 - +0.4 1,190,360 - -0.1 1,190,360 - +2.0 1,190,360 - +2.0 1,190,361 + +0.7 1,190,361 + +2.0 1,190,361 + -1.7 1,190,453 + MMJJ_RS06445 0.12 +0.5 1,190,453 + MMJJ_RS06445 0.12 +2.0 1,190,461 - MMJJ_RS06445 0.13 -0.8 1,190,461 - MMJJ_RS06445 0.13 -1.0 1,190,471 - MMJJ_RS06445 0.14 -1.6 1,190,471 - MMJJ_RS06445 0.14 +0.8 1,190,472 + MMJJ_RS06445 0.14 +1.0 1,190,472 + MMJJ_RS06445 0.14 -3.5 1,190,492 + MMJJ_RS06445 0.16 +2.1 1,190,518 - MMJJ_RS06445 0.20 +1.0 1,190,591 - MMJJ_RS06445 0.28 -1.4 1,190,591 - MMJJ_RS06445 0.28 +0.0 1,190,591 - MMJJ_RS06445 0.28 +0.9 1,190,591 - MMJJ_RS06445 0.28 +2.6 1,190,591 - MMJJ_RS06445 0.28 +0.6 1,190,591 - MMJJ_RS06445 0.28 +1.7 1,190,592 + MMJJ_RS06445 0.29 -0.1 1,190,592 + MMJJ_RS06445 0.29 +1.4 1,190,592 + MMJJ_RS06445 0.29 -1.2 1,190,592 + MMJJ_RS06445 0.29 +0.7 1,190,596 - MMJJ_RS06445 0.29 +2.1 1,190,597 + MMJJ_RS06445 0.29 -0.4 1,190,597 + MMJJ_RS06445 0.29 +2.6 1,190,597 + MMJJ_RS06445 0.29 -0.0 1,190,609 - MMJJ_RS06445 0.31 +1.0 1,190,610 + MMJJ_RS06445 0.31 -0.2 1,190,610 + MMJJ_RS06445 0.31 +1.5 1,190,615 + MMJJ_RS06445 0.31 -3.8 1,190,623 - MMJJ_RS06445 0.32 +2.0 1,190,623 - MMJJ_RS06445 0.32 +1.5 1,190,623 - MMJJ_RS06445 0.32 -2.4 1,190,624 + MMJJ_RS06445 0.32 +1.0 1,190,624 + MMJJ_RS06445 0.32 -0.1 1,190,624 + MMJJ_RS06445 0.32 +2.0 1,190,633 - MMJJ_RS06445 0.34 +2.7 1,190,633 - MMJJ_RS06445 0.34 +2.1 1,190,634 + MMJJ_RS06445 0.34 +0.9 1,190,634 + MMJJ_RS06445 0.34 +1.6 1,190,662 - MMJJ_RS06445 0.37 +0.4 1,190,662 - MMJJ_RS06445 0.37 +0.2 1,190,662 - MMJJ_RS06445 0.37 +0.2 1,190,662 - MMJJ_RS06445 0.37 +1.6 1,190,662 - MMJJ_RS06445 0.37 +1.4 1,190,662 - MMJJ_RS06445 0.37 +0.5 1,190,662 - MMJJ_RS06445 0.37 +0.1 1,190,663 + MMJJ_RS06445 0.37 +1.3 1,190,663 + MMJJ_RS06445 0.37 -1.4 1,190,663 + MMJJ_RS06445 0.37 +0.3 1,190,663 + MMJJ_RS06445 0.37 +1.1 1,190,664 - MMJJ_RS06445 0.37 +0.2 1,190,665 + MMJJ_RS06445 0.37 +0.0 1,190,665 + MMJJ_RS06445 0.37 +1.2 1,190,665 + MMJJ_RS06445 0.37 +1.6 1,190,713 - MMJJ_RS06445 0.43 -2.2 1,190,723 - MMJJ_RS06445 0.45 +0.2 1,190,723 - MMJJ_RS06445 0.45 -0.1 1,190,723 - MMJJ_RS06445 0.45 -0.4 1,190,723 - MMJJ_RS06445 0.45 -0.4 1,190,723 - MMJJ_RS06445 0.45 -2.1 1,190,723 - MMJJ_RS06445 0.45 -0.3 1,190,723 - MMJJ_RS06445 0.45 -0.0 1,190,723 - MMJJ_RS06445 0.45 +0.9 1,190,724 + MMJJ_RS06445 0.45 +0.0 1,190,724 + MMJJ_RS06445 0.45 +1.1 1,190,724 + MMJJ_RS06445 0.45 +1.5 1,190,727 - MMJJ_RS06445 0.45 +1.7 1,190,727 - MMJJ_RS06445 0.45 -0.3 1,190,727 - MMJJ_RS06445 0.45 -0.7 1,190,727 - MMJJ_RS06445 0.45 -0.2 1,190,727 - MMJJ_RS06445 0.45 -1.6 1,190,728 + MMJJ_RS06445 0.45 -1.0 1,190,800 - MMJJ_RS06445 0.54 +0.4 1,190,829 - MMJJ_RS06445 0.57 -1.9 1,190,829 - MMJJ_RS06445 0.57 -1.4 1,190,829 - MMJJ_RS06445 0.57 +1.2 1,190,829 - MMJJ_RS06445 0.57 +1.0 1,190,830 + MMJJ_RS06445 0.58 +2.3 1,190,830 + MMJJ_RS06445 0.58 +0.6 1,190,830 + MMJJ_RS06445 0.58 -0.1
Or see this region's nucleotide sequence