Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09030 and MMJJ_RS09035 are separated by 162 nucleotides MMJJ_RS09035 and MMJJ_RS09040 are separated by 118 nucleotides MMJJ_RS09040 and MMJJ_RS09045 are separated by 52 nucleotides
MMJJ_RS09030: MMJJ_RS09030 - hypothetical protein, at 1,665,329 to 1,668,259
_RS09030
MMJJ_RS09035: MMJJ_RS09035 - OB-fold nucleic acid binding domain-containing protein, at 1,668,422 to 1,670,350
_RS09035
MMJJ_RS09040: MMJJ_RS09040 - adenylate kinase, at 1,670,469 to 1,671,047
_RS09040
MMJJ_RS09045: MMJJ_RS09045 - EMC3/TMCO1 family protein, at 1,671,100 to 1,671,696
_RS09045
Position (kb)
1668
1669
1670
1671 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1667.441 kb on - strand, within MMJJ_RS09030 at 1667.474 kb on - strand, within MMJJ_RS09030 at 1667.555 kb on + strand, within MMJJ_RS09030 at 1667.649 kb on - strand, within MMJJ_RS09030 at 1667.649 kb on - strand, within MMJJ_RS09030 at 1667.684 kb on - strand, within MMJJ_RS09030 at 1667.684 kb on - strand, within MMJJ_RS09030 at 1667.684 kb on - strand, within MMJJ_RS09030 at 1667.685 kb on + strand, within MMJJ_RS09030 at 1667.685 kb on + strand, within MMJJ_RS09030 at 1667.685 kb on + strand, within MMJJ_RS09030 at 1667.685 kb on + strand, within MMJJ_RS09030 at 1667.688 kb on - strand, within MMJJ_RS09030 at 1667.691 kb on + strand, within MMJJ_RS09030 at 1667.691 kb on + strand, within MMJJ_RS09030 at 1667.702 kb on - strand, within MMJJ_RS09030 at 1667.702 kb on - strand, within MMJJ_RS09030 at 1667.709 kb on + strand, within MMJJ_RS09030 at 1667.789 kb on + strand, within MMJJ_RS09030 at 1667.818 kb on - strand, within MMJJ_RS09030 at 1667.819 kb on + strand, within MMJJ_RS09030 at 1667.864 kb on - strand, within MMJJ_RS09030 at 1667.865 kb on + strand, within MMJJ_RS09030 at 1667.886 kb on - strand, within MMJJ_RS09030 at 1667.905 kb on - strand, within MMJJ_RS09030 at 1667.924 kb on + strand, within MMJJ_RS09030 at 1667.926 kb on - strand, within MMJJ_RS09030 at 1667.928 kb on - strand, within MMJJ_RS09030 at 1667.929 kb on + strand, within MMJJ_RS09030 at 1667.929 kb on + strand, within MMJJ_RS09030 at 1667.948 kb on - strand, within MMJJ_RS09030 at 1667.960 kb on + strand, within MMJJ_RS09030 at 1667.964 kb on + strand, within MMJJ_RS09030 at 1667.987 kb on - strand at 1668.004 kb on + strand at 1668.045 kb on + strand at 1668.068 kb on + strand at 1668.127 kb on - strand at 1668.131 kb on - strand at 1668.137 kb on + strand at 1668.140 kb on + strand at 1668.140 kb on + strand at 1668.144 kb on + strand at 1668.171 kb on - strand at 1668.195 kb on + strand at 1668.251 kb on + strand at 1668.251 kb on + strand at 1668.271 kb on - strand at 1668.272 kb on + strand at 1668.272 kb on + strand at 1668.285 kb on + strand at 1668.288 kb on - strand at 1668.288 kb on - strand at 1668.288 kb on - strand at 1668.288 kb on - strand at 1668.289 kb on + strand at 1668.289 kb on + strand at 1668.290 kb on - strand at 1668.290 kb on - strand at 1668.290 kb on - strand at 1668.290 kb on - strand at 1668.290 kb on - strand at 1668.291 kb on + strand at 1668.292 kb on - strand at 1668.292 kb on - strand at 1668.292 kb on - strand at 1668.293 kb on + strand at 1668.293 kb on + strand at 1668.293 kb on + strand at 1668.301 kb on - strand at 1668.302 kb on + strand at 1668.313 kb on + strand at 1668.313 kb on + strand at 1668.314 kb on - strand at 1668.314 kb on - strand at 1668.314 kb on - strand at 1668.314 kb on - strand at 1668.314 kb on - strand at 1668.315 kb on + strand at 1668.315 kb on + strand at 1668.315 kb on + strand at 1668.315 kb on + strand at 1668.315 kb on + strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.319 kb on - strand at 1668.319 kb on - strand at 1668.319 kb on - strand at 1668.319 kb on - strand at 1668.320 kb on + strand at 1668.321 kb on - strand at 1668.321 kb on - strand at 1668.321 kb on - strand at 1668.322 kb on + strand at 1668.353 kb on - strand at 1668.357 kb on + strand at 1668.358 kb on - strand at 1668.369 kb on - strand at 1668.370 kb on + strand at 1668.382 kb on - strand at 1669.084 kb on - strand at 1669.210 kb on + strand, within MMJJ_RS09035 at 1669.267 kb on - strand, within MMJJ_RS09035 at 1669.267 kb on - strand, within MMJJ_RS09035 at 1669.286 kb on - strand, within MMJJ_RS09035 at 1669.287 kb on + strand, within MMJJ_RS09035 at 1669.287 kb on + strand, within MMJJ_RS09035 at 1669.378 kb on + strand at 1669.378 kb on + strand, within MMJJ_RS09035 at 1669.461 kb on - strand, within MMJJ_RS09035 at 1669.549 kb on - strand, within MMJJ_RS09035 at 1669.639 kb on + strand, within MMJJ_RS09035 at 1669.639 kb on + strand, within MMJJ_RS09035 at 1669.639 kb on + strand, within MMJJ_RS09035 at 1669.728 kb on - strand, within MMJJ_RS09035 at 1669.750 kb on + strand, within MMJJ_RS09035 at 1670.008 kb on + strand at 1670.052 kb on + strand, within MMJJ_RS09035 at 1670.141 kb on + strand, within MMJJ_RS09035 at 1670.244 kb on - strand at 1670.292 kb on - strand at 1670.316 kb on - strand at 1670.316 kb on - strand at 1670.316 kb on - strand at 1670.316 kb on - strand at 1670.316 kb on - strand at 1670.317 kb on + strand at 1670.317 kb on + strand at 1670.317 kb on + strand at 1670.382 kb on - strand at 1670.382 kb on - strand at 1670.386 kb on - strand at 1671.050 kb on - strand at 1671.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 1,667,441 - MMJJ_RS09030 0.72 -2.0 1,667,474 - MMJJ_RS09030 0.73 -0.2 1,667,555 + MMJJ_RS09030 0.76 +1.6 1,667,649 - MMJJ_RS09030 0.79 +0.8 1,667,649 - MMJJ_RS09030 0.79 -0.2 1,667,684 - MMJJ_RS09030 0.80 -0.2 1,667,684 - MMJJ_RS09030 0.80 -1.2 1,667,684 - MMJJ_RS09030 0.80 -2.8 1,667,685 + MMJJ_RS09030 0.80 +0.8 1,667,685 + MMJJ_RS09030 0.80 -2.0 1,667,685 + MMJJ_RS09030 0.80 -2.0 1,667,685 + MMJJ_RS09030 0.80 -0.8 1,667,688 - MMJJ_RS09030 0.80 -2.2 1,667,691 + MMJJ_RS09030 0.81 -0.8 1,667,691 + MMJJ_RS09030 0.81 -0.2 1,667,702 - MMJJ_RS09030 0.81 -0.2 1,667,702 - MMJJ_RS09030 0.81 -1.2 1,667,709 + MMJJ_RS09030 0.81 -1.5 1,667,789 + MMJJ_RS09030 0.84 -1.4 1,667,818 - MMJJ_RS09030 0.85 -2.2 1,667,819 + MMJJ_RS09030 0.85 -2.0 1,667,864 - MMJJ_RS09030 0.86 -1.5 1,667,865 + MMJJ_RS09030 0.87 -1.5 1,667,886 - MMJJ_RS09030 0.87 -2.2 1,667,905 - MMJJ_RS09030 0.88 -1.5 1,667,924 + MMJJ_RS09030 0.89 -1.5 1,667,926 - MMJJ_RS09030 0.89 +0.8 1,667,928 - MMJJ_RS09030 0.89 -0.2 1,667,929 + MMJJ_RS09030 0.89 -1.8 1,667,929 + MMJJ_RS09030 0.89 +1.1 1,667,948 - MMJJ_RS09030 0.89 +2.1 1,667,960 + MMJJ_RS09030 0.90 -0.2 1,667,964 + MMJJ_RS09030 0.90 -0.8 1,667,987 - +0.8 1,668,004 + +0.8 1,668,045 + -1.5 1,668,068 + -1.2 1,668,127 - -1.0 1,668,131 - -1.5 1,668,137 + -0.8 1,668,140 + -1.2 1,668,140 + -1.2 1,668,144 + -1.2 1,668,171 - -0.2 1,668,195 + -0.8 1,668,251 + -0.8 1,668,251 + -1.2 1,668,271 - -1.5 1,668,272 + -2.7 1,668,272 + -3.2 1,668,285 + -2.9 1,668,288 - +1.7 1,668,288 - -0.8 1,668,288 - -0.8 1,668,288 - -2.7 1,668,289 + -3.4 1,668,289 + -0.2 1,668,290 - -2.0 1,668,290 - +1.6 1,668,290 - -0.1 1,668,290 - -1.5 1,668,290 - -2.9 1,668,291 + -2.3 1,668,292 - -0.2 1,668,292 - -3.3 1,668,292 - -0.2 1,668,293 + +0.8 1,668,293 + -2.4 1,668,293 + -0.3 1,668,301 - +0.1 1,668,302 + +2.4 1,668,313 + -0.5 1,668,313 + +0.1 1,668,314 - -2.1 1,668,314 - -3.1 1,668,314 - -0.8 1,668,314 - -2.5 1,668,314 - +0.6 1,668,315 + -0.2 1,668,315 + -1.0 1,668,315 + +1.1 1,668,315 + -0.5 1,668,315 + +2.4 1,668,317 - -2.4 1,668,317 - -3.6 1,668,317 - -0.5 1,668,317 - -0.8 1,668,317 - -1.4 1,668,317 - -0.1 1,668,317 - -2.7 1,668,317 - +2.2 1,668,318 + -2.0 1,668,318 + -2.2 1,668,318 + -2.7 1,668,318 + +0.3 1,668,318 + +0.3 1,668,318 + -1.2 1,668,318 + -3.5 1,668,318 + -0.2 1,668,318 + +0.1 1,668,318 + -3.4 1,668,318 + +1.1 1,668,318 + -1.8 1,668,318 + -0.8 1,668,319 - -1.5 1,668,319 - -0.9 1,668,319 - -0.5 1,668,319 - -3.4 1,668,320 + -0.5 1,668,321 - -2.9 1,668,321 - -2.2 1,668,321 - -0.1 1,668,322 + -3.4 1,668,353 - -0.8 1,668,357 + -1.5 1,668,358 - -1.5 1,668,369 - -0.2 1,668,370 + -0.8 1,668,382 - +2.8 1,669,084 - -0.8 1,669,210 + MMJJ_RS09035 0.41 -1.8 1,669,267 - MMJJ_RS09035 0.44 -0.8 1,669,267 - MMJJ_RS09035 0.44 -1.5 1,669,286 - MMJJ_RS09035 0.45 -1.8 1,669,287 + MMJJ_RS09035 0.45 -3.5 1,669,287 + MMJJ_RS09035 0.45 -2.0 1,669,378 + +0.8 1,669,378 + MMJJ_RS09035 0.50 -0.2 1,669,461 - MMJJ_RS09035 0.54 -1.5 1,669,549 - MMJJ_RS09035 0.58 -0.1 1,669,639 + MMJJ_RS09035 0.63 -2.4 1,669,639 + MMJJ_RS09035 0.63 -3.2 1,669,639 + MMJJ_RS09035 0.63 -3.2 1,669,728 - MMJJ_RS09035 0.68 -0.2 1,669,750 + MMJJ_RS09035 0.69 -0.8 1,670,008 + -0.2 1,670,052 + MMJJ_RS09035 0.84 -2.2 1,670,141 + MMJJ_RS09035 0.89 -1.5 1,670,244 - -1.2 1,670,292 - -1.5 1,670,316 - -4.1 1,670,316 - -0.5 1,670,316 - -0.3 1,670,316 - -0.6 1,670,316 - +1.5 1,670,317 + +0.8 1,670,317 + +0.3 1,670,317 + -0.8 1,670,382 - -0.2 1,670,382 - -2.0 1,670,386 - -0.8 1,671,050 - -0.2 1,671,056 - -2.0
Or see this region's nucleotide sequence