Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07930 and MMJJ_RS07935 are separated by 22 nucleotides MMJJ_RS07935 and MMJJ_RS07940 are separated by 40 nucleotides MMJJ_RS07940 and MMJJ_RS07945 are separated by 22 nucleotides
MMJJ_RS07930: MMJJ_RS07930 - formylmethanofuran dehydrogenase subunit A, at 1,455,112 to 1,456,815
_RS07930
MMJJ_RS07935: MMJJ_RS07935 - protein fwdD, at 1,456,838 to 1,457,224
_RS07935
MMJJ_RS07940: MMJJ_RS07940 - 4Fe-4S binding protein, at 1,457,265 to 1,457,507
_RS07940
MMJJ_RS07945: MMJJ_RS07945 - 4Fe-4S binding protein, at 1,457,530 to 1,458,588
_RS07945
Position (kb) 1456
1457
1458 Strain fitness (log2 ratio) -2
-1
0
1 at 1455.946 kb on - strand, within MMJJ_RS07930 at 1455.958 kb on - strand, within MMJJ_RS07930 at 1455.959 kb on + strand at 1456.369 kb on - strand, within MMJJ_RS07930 at 1456.802 kb on + strand at 1456.951 kb on - strand, within MMJJ_RS07935 at 1456.998 kb on + strand, within MMJJ_RS07935 at 1457.418 kb on - strand, within MMJJ_RS07940 at 1457.490 kb on - strand at 1457.491 kb on + strand at 1457.518 kb on + strand at 1457.531 kb on - strand at 1457.590 kb on - strand at 1457.655 kb on - strand, within MMJJ_RS07945 at 1457.655 kb on - strand at 1457.660 kb on + strand, within MMJJ_RS07945 at 1457.682 kb on - strand at 1457.686 kb on - strand, within MMJJ_RS07945 at 1458.002 kb on - strand, within MMJJ_RS07945 at 1458.016 kb on - strand, within MMJJ_RS07945 at 1458.053 kb on + strand at 1458.122 kb on + strand, within MMJJ_RS07945
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 1,455,946 - MMJJ_RS07930 0.49 -1.5 1,455,958 - MMJJ_RS07930 0.50 -0.7 1,455,959 + -0.7 1,456,369 - MMJJ_RS07930 0.74 -1.5 1,456,802 + -0.7 1,456,951 - MMJJ_RS07935 0.29 -1.8 1,456,998 + MMJJ_RS07935 0.41 -0.7 1,457,418 - MMJJ_RS07940 0.63 -1.1 1,457,490 - -1.9 1,457,491 + +0.9 1,457,518 + +0.9 1,457,531 - +0.9 1,457,590 - -0.7 1,457,655 - MMJJ_RS07945 0.12 -1.5 1,457,655 - +0.9 1,457,660 + MMJJ_RS07945 0.12 -0.7 1,457,682 - -0.7 1,457,686 - MMJJ_RS07945 0.15 -1.1 1,458,002 - MMJJ_RS07945 0.45 +0.9 1,458,016 - MMJJ_RS07945 0.46 +0.9 1,458,053 + -1.5 1,458,122 + MMJJ_RS07945 0.56 -1.7
Or see this region's nucleotide sequence