Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07620 and MMJJ_RS07625 are separated by 62 nucleotides MMJJ_RS07625 and MMJJ_RS07630 are separated by 23 nucleotides MMJJ_RS07630 and MMJJ_RS07635 are separated by 215 nucleotides MMJJ_RS07635 and MMJJ_RS07640 are separated by 27 nucleotides
MMJJ_RS07620: MMJJ_RS07620 - class I SAM-dependent rRNA methyltransferase, at 1,393,410 to 1,394,552
_RS07620
MMJJ_RS07625: MMJJ_RS07625 - phosphopantothenate/pantothenate synthetase, at 1,394,615 to 1,395,376
_RS07625
MMJJ_RS07630: MMJJ_RS07630 - hypothetical protein, at 1,395,400 to 1,396,803
_RS07630
MMJJ_RS07635: MMJJ_RS07635 - response regulator transcription factor, at 1,397,019 to 1,397,378
_RS07635
MMJJ_RS07640: MMJJ_RS07640 - cache domain-containing protein, at 1,397,406 to 1,399,325
_RS07640
Position (kb) 1395
1396
1397 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 1394.488 kb on - strand at 1394.488 kb on - strand at 1394.488 kb on - strand at 1394.488 kb on - strand at 1394.488 kb on - strand at 1394.491 kb on - strand at 1394.491 kb on - strand at 1394.491 kb on - strand at 1394.504 kb on - strand at 1394.505 kb on + strand at 1394.505 kb on + strand at 1394.518 kb on + strand at 1394.543 kb on + strand at 1394.545 kb on - strand at 1394.571 kb on + strand at 1394.805 kb on - strand, within MMJJ_RS07625 at 1394.896 kb on + strand, within MMJJ_RS07625 at 1395.249 kb on - strand at 1395.328 kb on - strand at 1395.354 kb on - strand at 1395.414 kb on - strand at 1395.445 kb on - strand at 1395.445 kb on - strand at 1395.498 kb on - strand at 1395.498 kb on - strand at 1395.504 kb on + strand at 1395.504 kb on + strand at 1395.504 kb on + strand at 1395.504 kb on + strand at 1395.520 kb on - strand at 1395.521 kb on + strand at 1395.581 kb on + strand, within MMJJ_RS07630 at 1395.597 kb on - strand, within MMJJ_RS07630 at 1395.597 kb on - strand, within MMJJ_RS07630 at 1395.640 kb on - strand, within MMJJ_RS07630 at 1395.640 kb on - strand, within MMJJ_RS07630 at 1395.641 kb on + strand, within MMJJ_RS07630 at 1395.641 kb on + strand, within MMJJ_RS07630 at 1395.641 kb on + strand, within MMJJ_RS07630 at 1395.641 kb on + strand, within MMJJ_RS07630 at 1395.645 kb on - strand, within MMJJ_RS07630 at 1395.673 kb on + strand, within MMJJ_RS07630 at 1395.675 kb on - strand, within MMJJ_RS07630 at 1395.675 kb on - strand, within MMJJ_RS07630 at 1395.675 kb on - strand, within MMJJ_RS07630 at 1395.675 kb on - strand, within MMJJ_RS07630 at 1395.676 kb on + strand, within MMJJ_RS07630 at 1395.676 kb on + strand, within MMJJ_RS07630 at 1395.676 kb on + strand, within MMJJ_RS07630 at 1395.676 kb on + strand, within MMJJ_RS07630 at 1395.676 kb on + strand, within MMJJ_RS07630 at 1395.846 kb on - strand, within MMJJ_RS07630 at 1395.874 kb on + strand, within MMJJ_RS07630 at 1395.952 kb on - strand, within MMJJ_RS07630 at 1395.985 kb on + strand, within MMJJ_RS07630 at 1396.030 kb on - strand, within MMJJ_RS07630 at 1396.034 kb on - strand, within MMJJ_RS07630 at 1396.064 kb on + strand, within MMJJ_RS07630 at 1396.076 kb on - strand, within MMJJ_RS07630 at 1396.092 kb on - strand, within MMJJ_RS07630 at 1396.120 kb on - strand, within MMJJ_RS07630 at 1396.153 kb on + strand, within MMJJ_RS07630 at 1396.157 kb on + strand, within MMJJ_RS07630 at 1396.194 kb on + strand, within MMJJ_RS07630 at 1396.197 kb on - strand, within MMJJ_RS07630 at 1396.197 kb on - strand, within MMJJ_RS07630 at 1396.198 kb on + strand, within MMJJ_RS07630 at 1396.198 kb on + strand, within MMJJ_RS07630 at 1396.219 kb on - strand, within MMJJ_RS07630 at 1396.219 kb on - strand, within MMJJ_RS07630 at 1396.219 kb on - strand, within MMJJ_RS07630 at 1396.219 kb on - strand, within MMJJ_RS07630 at 1396.219 kb on - strand, within MMJJ_RS07630 at 1396.220 kb on + strand, within MMJJ_RS07630 at 1396.220 kb on + strand, within MMJJ_RS07630 at 1396.220 kb on + strand, within MMJJ_RS07630 at 1396.251 kb on - strand, within MMJJ_RS07630 at 1396.252 kb on + strand, within MMJJ_RS07630 at 1396.255 kb on - strand, within MMJJ_RS07630 at 1396.255 kb on - strand, within MMJJ_RS07630 at 1396.256 kb on + strand, within MMJJ_RS07630 at 1396.256 kb on + strand, within MMJJ_RS07630 at 1396.259 kb on - strand, within MMJJ_RS07630 at 1396.259 kb on - strand, within MMJJ_RS07630 at 1396.281 kb on + strand, within MMJJ_RS07630 at 1396.281 kb on + strand, within MMJJ_RS07630 at 1396.281 kb on + strand, within MMJJ_RS07630 at 1396.284 kb on - strand, within MMJJ_RS07630 at 1396.284 kb on - strand, within MMJJ_RS07630 at 1396.285 kb on + strand, within MMJJ_RS07630 at 1396.285 kb on + strand, within MMJJ_RS07630 at 1396.285 kb on + strand, within MMJJ_RS07630 at 1396.285 kb on + strand, within MMJJ_RS07630 at 1396.336 kb on + strand, within MMJJ_RS07630 at 1396.353 kb on - strand, within MMJJ_RS07630 at 1396.396 kb on - strand, within MMJJ_RS07630 at 1396.399 kb on - strand, within MMJJ_RS07630 at 1396.399 kb on - strand, within MMJJ_RS07630 at 1396.399 kb on - strand, within MMJJ_RS07630 at 1396.399 kb on - strand, within MMJJ_RS07630 at 1396.400 kb on + strand, within MMJJ_RS07630 at 1396.403 kb on - strand at 1396.403 kb on - strand, within MMJJ_RS07630 at 1396.403 kb on - strand, within MMJJ_RS07630 at 1396.404 kb on + strand, within MMJJ_RS07630 at 1396.446 kb on - strand, within MMJJ_RS07630 at 1396.447 kb on + strand, within MMJJ_RS07630 at 1396.598 kb on + strand, within MMJJ_RS07630 at 1396.638 kb on - strand, within MMJJ_RS07630 at 1396.776 kb on - strand at 1396.776 kb on - strand at 1396.825 kb on + strand at 1396.825 kb on + strand at 1396.829 kb on + strand at 1396.831 kb on - strand at 1396.857 kb on + strand at 1396.862 kb on - strand at 1396.863 kb on + strand at 1396.864 kb on - strand at 1396.864 kb on - strand at 1396.865 kb on + strand at 1396.865 kb on + strand at 1396.866 kb on - strand at 1396.866 kb on - strand at 1396.867 kb on + strand at 1396.867 kb on + strand at 1396.867 kb on + strand at 1396.875 kb on - strand at 1396.886 kb on - strand at 1396.886 kb on - strand at 1396.886 kb on - strand at 1396.887 kb on + strand at 1396.889 kb on - strand at 1396.889 kb on - strand at 1396.890 kb on + strand at 1396.891 kb on - strand at 1396.892 kb on + strand at 1396.892 kb on + strand at 1396.913 kb on - strand at 1396.913 kb on - strand at 1396.918 kb on - strand at 1396.918 kb on - strand at 1396.918 kb on - strand at 1396.918 kb on - strand at 1396.919 kb on + strand at 1396.919 kb on + strand at 1396.919 kb on + strand at 1396.930 kb on + strand at 1396.930 kb on + strand at 1396.930 kb on + strand at 1396.930 kb on + strand at 1396.930 kb on + strand at 1396.930 kb on + strand at 1396.933 kb on - strand at 1396.933 kb on - strand at 1396.934 kb on + strand at 1396.987 kb on - strand at 1396.991 kb on + strand at 1396.996 kb on + strand at 1396.996 kb on + strand at 1396.996 kb on + strand at 1397.032 kb on - strand at 1397.035 kb on - strand at 1397.035 kb on - strand at 1397.054 kb on - strand at 1397.054 kb on - strand at 1397.055 kb on + strand, within MMJJ_RS07635 at 1397.055 kb on + strand, within MMJJ_RS07635 at 1397.055 kb on + strand, within MMJJ_RS07635 at 1397.056 kb on - strand, within MMJJ_RS07635 at 1397.056 kb on - strand, within MMJJ_RS07635 at 1397.057 kb on + strand, within MMJJ_RS07635 at 1397.057 kb on + strand, within MMJJ_RS07635 at 1397.059 kb on - strand, within MMJJ_RS07635 at 1397.060 kb on + strand, within MMJJ_RS07635 at 1397.075 kb on - strand, within MMJJ_RS07635 at 1397.088 kb on + strand, within MMJJ_RS07635 at 1397.091 kb on + strand, within MMJJ_RS07635 at 1397.094 kb on - strand, within MMJJ_RS07635 at 1397.094 kb on - strand, within MMJJ_RS07635 at 1397.094 kb on - strand, within MMJJ_RS07635 at 1397.095 kb on + strand, within MMJJ_RS07635 at 1397.103 kb on + strand, within MMJJ_RS07635 at 1397.103 kb on + strand, within MMJJ_RS07635 at 1397.103 kb on + strand, within MMJJ_RS07635 at 1397.160 kb on + strand, within MMJJ_RS07635 at 1397.164 kb on - strand, within MMJJ_RS07635 at 1397.174 kb on - strand, within MMJJ_RS07635 at 1397.206 kb on - strand, within MMJJ_RS07635 at 1397.211 kb on + strand, within MMJJ_RS07635 at 1397.216 kb on - strand, within MMJJ_RS07635 at 1397.229 kb on - strand, within MMJJ_RS07635 at 1397.235 kb on + strand, within MMJJ_RS07635 at 1397.292 kb on + strand, within MMJJ_RS07635 at 1397.317 kb on - strand, within MMJJ_RS07635 at 1397.318 kb on + strand, within MMJJ_RS07635 at 1397.342 kb on + strand, within MMJJ_RS07635 at 1397.383 kb on - strand at 1397.450 kb on + strand at 1397.473 kb on - strand at 1397.474 kb on + strand at 1397.474 kb on + strand at 1397.582 kb on + strand at 1397.618 kb on - strand, within MMJJ_RS07640 at 1397.618 kb on - strand, within MMJJ_RS07640 at 1397.619 kb on + strand, within MMJJ_RS07640 at 1397.622 kb on - strand, within MMJJ_RS07640 at 1397.622 kb on - strand, within MMJJ_RS07640 at 1397.622 kb on - strand, within MMJJ_RS07640 at 1397.623 kb on + strand, within MMJJ_RS07640 at 1397.623 kb on + strand, within MMJJ_RS07640 at 1397.624 kb on - strand, within MMJJ_RS07640 at 1397.652 kb on + strand, within MMJJ_RS07640 at 1397.669 kb on - strand, within MMJJ_RS07640 at 1397.696 kb on + strand, within MMJJ_RS07640 at 1397.709 kb on + strand, within MMJJ_RS07640 at 1397.709 kb on + strand, within MMJJ_RS07640 at 1397.796 kb on - strand, within MMJJ_RS07640
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 1,394,488 - -2.8 1,394,488 - +1.7 1,394,488 - +0.5 1,394,488 - +2.2 1,394,488 - -2.4 1,394,491 - +1.7 1,394,491 - -0.0 1,394,491 - +0.5 1,394,504 - +0.7 1,394,505 + +5.5 1,394,505 + +1.0 1,394,518 + +2.9 1,394,543 + -0.8 1,394,545 - +0.1 1,394,571 + +1.8 1,394,805 - MMJJ_RS07625 0.25 -0.5 1,394,896 + MMJJ_RS07625 0.37 -0.5 1,395,249 - +1.0 1,395,328 - -2.1 1,395,354 - -1.8 1,395,414 - -0.5 1,395,445 - -3.3 1,395,445 - +1.0 1,395,498 - +0.0 1,395,498 - -1.5 1,395,504 + -1.3 1,395,504 + -2.7 1,395,504 + -2.4 1,395,504 + -0.5 1,395,520 - -1.8 1,395,521 + -2.3 1,395,581 + MMJJ_RS07630 0.13 -2.0 1,395,597 - MMJJ_RS07630 0.14 -1.5 1,395,597 - MMJJ_RS07630 0.14 -1.3 1,395,640 - MMJJ_RS07630 0.17 -1.5 1,395,640 - MMJJ_RS07630 0.17 -2.0 1,395,641 + MMJJ_RS07630 0.17 +1.5 1,395,641 + MMJJ_RS07630 0.17 -0.1 1,395,641 + MMJJ_RS07630 0.17 -1.0 1,395,641 + MMJJ_RS07630 0.17 -1.3 1,395,645 - MMJJ_RS07630 0.17 -2.0 1,395,673 + MMJJ_RS07630 0.19 -2.2 1,395,675 - MMJJ_RS07630 0.20 +0.0 1,395,675 - MMJJ_RS07630 0.20 -2.1 1,395,675 - MMJJ_RS07630 0.20 -2.1 1,395,675 - MMJJ_RS07630 0.20 -1.8 1,395,676 + MMJJ_RS07630 0.20 -1.3 1,395,676 + MMJJ_RS07630 0.20 -2.5 1,395,676 + MMJJ_RS07630 0.20 -2.0 1,395,676 + MMJJ_RS07630 0.20 -3.5 1,395,676 + MMJJ_RS07630 0.20 -2.9 1,395,846 - MMJJ_RS07630 0.32 -2.4 1,395,874 + MMJJ_RS07630 0.34 -1.3 1,395,952 - MMJJ_RS07630 0.39 -1.3 1,395,985 + MMJJ_RS07630 0.42 -2.8 1,396,030 - MMJJ_RS07630 0.45 +1.8 1,396,034 - MMJJ_RS07630 0.45 -0.3 1,396,064 + MMJJ_RS07630 0.47 -1.5 1,396,076 - MMJJ_RS07630 0.48 -2.5 1,396,092 - MMJJ_RS07630 0.49 +0.5 1,396,120 - MMJJ_RS07630 0.51 -2.4 1,396,153 + MMJJ_RS07630 0.54 -1.0 1,396,157 + MMJJ_RS07630 0.54 -0.7 1,396,194 + MMJJ_RS07630 0.57 -2.1 1,396,197 - MMJJ_RS07630 0.57 -1.5 1,396,197 - MMJJ_RS07630 0.57 -1.0 1,396,198 + MMJJ_RS07630 0.57 +1.0 1,396,198 + MMJJ_RS07630 0.57 -3.0 1,396,219 - MMJJ_RS07630 0.58 +0.0 1,396,219 - MMJJ_RS07630 0.58 -3.9 1,396,219 - MMJJ_RS07630 0.58 -2.4 1,396,219 - MMJJ_RS07630 0.58 -2.0 1,396,219 - MMJJ_RS07630 0.58 +2.0 1,396,220 + MMJJ_RS07630 0.58 -1.1 1,396,220 + MMJJ_RS07630 0.58 -1.5 1,396,220 + MMJJ_RS07630 0.58 -1.5 1,396,251 - MMJJ_RS07630 0.61 -1.3 1,396,252 + MMJJ_RS07630 0.61 -1.0 1,396,255 - MMJJ_RS07630 0.61 -1.0 1,396,255 - MMJJ_RS07630 0.61 -2.4 1,396,256 + MMJJ_RS07630 0.61 +3.3 1,396,256 + MMJJ_RS07630 0.61 -0.5 1,396,259 - MMJJ_RS07630 0.61 -2.1 1,396,259 - MMJJ_RS07630 0.61 -1.3 1,396,281 + MMJJ_RS07630 0.63 -2.1 1,396,281 + MMJJ_RS07630 0.63 -1.8 1,396,281 + MMJJ_RS07630 0.63 -2.1 1,396,284 - MMJJ_RS07630 0.63 -1.3 1,396,284 - MMJJ_RS07630 0.63 +0.5 1,396,285 + MMJJ_RS07630 0.63 -2.0 1,396,285 + MMJJ_RS07630 0.63 -2.3 1,396,285 + MMJJ_RS07630 0.63 -0.5 1,396,285 + MMJJ_RS07630 0.63 -2.1 1,396,336 + MMJJ_RS07630 0.67 -2.0 1,396,353 - MMJJ_RS07630 0.68 -2.0 1,396,396 - MMJJ_RS07630 0.71 -3.0 1,396,399 - MMJJ_RS07630 0.71 +0.0 1,396,399 - MMJJ_RS07630 0.71 -1.8 1,396,399 - MMJJ_RS07630 0.71 -1.8 1,396,399 - MMJJ_RS07630 0.71 -1.5 1,396,400 + MMJJ_RS07630 0.71 -1.0 1,396,403 - +1.0 1,396,403 - MMJJ_RS07630 0.71 -1.3 1,396,403 - MMJJ_RS07630 0.71 -2.1 1,396,404 + MMJJ_RS07630 0.72 -2.7 1,396,446 - MMJJ_RS07630 0.75 -1.3 1,396,447 + MMJJ_RS07630 0.75 -2.5 1,396,598 + MMJJ_RS07630 0.85 -2.0 1,396,638 - MMJJ_RS07630 0.88 -2.8 1,396,776 - -1.8 1,396,776 - -1.3 1,396,825 + -3.1 1,396,825 + -2.4 1,396,829 + +0.0 1,396,831 - -2.1 1,396,857 + -1.9 1,396,862 - -2.1 1,396,863 + -3.8 1,396,864 - -2.5 1,396,864 - -1.8 1,396,865 + -2.1 1,396,865 + -3.5 1,396,866 - -0.5 1,396,866 - +0.4 1,396,867 + -2.4 1,396,867 + -1.5 1,396,867 + -2.1 1,396,875 - +0.0 1,396,886 - -2.7 1,396,886 - -3.4 1,396,886 - -2.0 1,396,887 + -1.6 1,396,889 - -1.2 1,396,889 - -2.2 1,396,890 + -3.7 1,396,891 - -1.0 1,396,892 + -3.8 1,396,892 + -4.0 1,396,913 - -1.2 1,396,913 - +0.4 1,396,918 - -0.2 1,396,918 - +1.4 1,396,918 - -1.3 1,396,918 - -3.2 1,396,919 + -0.5 1,396,919 + -1.0 1,396,919 + +0.2 1,396,930 + -3.9 1,396,930 + +0.0 1,396,930 + -2.8 1,396,930 + -0.2 1,396,930 + -2.0 1,396,930 + -0.5 1,396,933 - -2.4 1,396,933 - -2.1 1,396,934 + -2.6 1,396,987 - +1.6 1,396,991 + +2.0 1,396,996 + +2.5 1,396,996 + +1.9 1,396,996 + +2.7 1,397,032 - +2.1 1,397,035 - +2.5 1,397,035 - +1.0 1,397,054 - +2.3 1,397,054 - +0.9 1,397,055 + MMJJ_RS07635 0.10 +2.0 1,397,055 + MMJJ_RS07635 0.10 +1.0 1,397,055 + MMJJ_RS07635 0.10 -1.0 1,397,056 - MMJJ_RS07635 0.10 +2.2 1,397,056 - MMJJ_RS07635 0.10 +1.9 1,397,057 + MMJJ_RS07635 0.11 +2.1 1,397,057 + MMJJ_RS07635 0.11 +1.0 1,397,059 - MMJJ_RS07635 0.11 +2.4 1,397,060 + MMJJ_RS07635 0.11 +2.2 1,397,075 - MMJJ_RS07635 0.16 +0.5 1,397,088 + MMJJ_RS07635 0.19 +1.4 1,397,091 + MMJJ_RS07635 0.20 +1.4 1,397,094 - MMJJ_RS07635 0.21 +1.2 1,397,094 - MMJJ_RS07635 0.21 +0.6 1,397,094 - MMJJ_RS07635 0.21 -1.2 1,397,095 + MMJJ_RS07635 0.21 +1.4 1,397,103 + MMJJ_RS07635 0.23 +2.9 1,397,103 + MMJJ_RS07635 0.23 +2.4 1,397,103 + MMJJ_RS07635 0.23 +1.6 1,397,160 + MMJJ_RS07635 0.39 +2.1 1,397,164 - MMJJ_RS07635 0.40 +1.3 1,397,174 - MMJJ_RS07635 0.43 +1.8 1,397,206 - MMJJ_RS07635 0.52 +2.3 1,397,211 + MMJJ_RS07635 0.53 +1.0 1,397,216 - MMJJ_RS07635 0.55 +2.4 1,397,229 - MMJJ_RS07635 0.58 +1.7 1,397,235 + MMJJ_RS07635 0.60 +2.0 1,397,292 + MMJJ_RS07635 0.76 +0.5 1,397,317 - MMJJ_RS07635 0.83 +3.3 1,397,318 + MMJJ_RS07635 0.83 +2.4 1,397,342 + MMJJ_RS07635 0.90 +0.2 1,397,383 - +1.3 1,397,450 + +1.6 1,397,473 - +1.2 1,397,474 + +1.5 1,397,474 + +1.8 1,397,582 + +1.2 1,397,618 - MMJJ_RS07640 0.11 -1.3 1,397,618 - MMJJ_RS07640 0.11 +0.7 1,397,619 + MMJJ_RS07640 0.11 +1.4 1,397,622 - MMJJ_RS07640 0.11 +0.4 1,397,622 - MMJJ_RS07640 0.11 +3.0 1,397,622 - MMJJ_RS07640 0.11 +1.0 1,397,623 + MMJJ_RS07640 0.11 +1.6 1,397,623 + MMJJ_RS07640 0.11 +1.0 1,397,624 - MMJJ_RS07640 0.11 +1.5 1,397,652 + MMJJ_RS07640 0.13 +1.4 1,397,669 - MMJJ_RS07640 0.14 +1.9 1,397,696 + MMJJ_RS07640 0.15 +1.5 1,397,709 + MMJJ_RS07640 0.16 +0.7 1,397,709 + MMJJ_RS07640 0.16 +1.7 1,397,796 - MMJJ_RS07640 0.20 +0.8
Or see this region's nucleotide sequence