Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS07280

Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS07275 and MMJJ_RS07280 are separated by 63 nucleotidesMMJJ_RS07280 and MMJJ_RS07285 are separated by 286 nucleotidesMMJJ_RS07285 and MMJJ_RS07290 are separated by 48 nucleotides MMJJ_RS07275: MMJJ_RS07275 - formate--phosphoribosylaminoimidazolecarboxamide ligase, at 1,332,540 to 1,333,625 _RS07275 MMJJ_RS07280: MMJJ_RS07280 - phosphomannomutase/phosphoglucomutase, at 1,333,689 to 1,335,002 _RS07280 MMJJ_RS07285: MMJJ_RS07285 - 30S ribosomal protein S10, at 1,335,289 to 1,335,597 _RS07285 MMJJ_RS07290: MMJJ_RS07290 - translation elongation factor EF-1 subunit alpha, at 1,335,646 to 1,336,932 _RS07290 Position (kb) 1333 1334 1335 1336Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1332.696 kb on + strand, within MMJJ_RS07275at 1332.735 kb on - strand, within MMJJ_RS07275at 1332.736 kb on + strand, within MMJJ_RS07275at 1333.205 kb on + strand, within MMJJ_RS07275at 1333.217 kb on - strand, within MMJJ_RS07275at 1333.324 kb on - strand, within MMJJ_RS07275at 1333.436 kb on + strand, within MMJJ_RS07275at 1333.436 kb on + strand, within MMJJ_RS07275at 1333.505 kb on - strand, within MMJJ_RS07275at 1333.617 kb on + strandat 1333.623 kb on - strandat 1333.624 kb on + strandat 1333.624 kb on + strandat 1333.659 kb on + strandat 1333.673 kb on - strandat 1333.679 kb on - strandat 1333.698 kb on - strandat 1333.719 kb on - strandat 1333.815 kb on + strandat 1334.002 kb on - strand, within MMJJ_RS07280at 1334.013 kb on - strand, within MMJJ_RS07280at 1334.050 kb on - strand, within MMJJ_RS07280at 1334.116 kb on - strand, within MMJJ_RS07280at 1334.116 kb on - strand, within MMJJ_RS07280at 1334.117 kb on + strand, within MMJJ_RS07280at 1334.136 kb on - strand, within MMJJ_RS07280at 1334.253 kb on - strand, within MMJJ_RS07280at 1334.254 kb on + strand, within MMJJ_RS07280at 1334.254 kb on + strand, within MMJJ_RS07280at 1334.358 kb on - strand, within MMJJ_RS07280at 1334.358 kb on - strand, within MMJJ_RS07280at 1334.385 kb on + strand, within MMJJ_RS07280at 1334.440 kb on + strand, within MMJJ_RS07280at 1334.444 kb on - strand, within MMJJ_RS07280at 1334.444 kb on - strand, within MMJJ_RS07280at 1334.568 kb on - strand, within MMJJ_RS07280at 1334.667 kb on - strand, within MMJJ_RS07280at 1334.716 kb on - strand, within MMJJ_RS07280at 1334.716 kb on - strand, within MMJJ_RS07280at 1334.782 kb on - strand, within MMJJ_RS07280at 1334.782 kb on - strand, within MMJJ_RS07280at 1334.782 kb on - strand, within MMJJ_RS07280at 1334.782 kb on - strand, within MMJJ_RS07280at 1334.783 kb on + strand, within MMJJ_RS07280at 1334.835 kb on - strand, within MMJJ_RS07280at 1334.847 kb on + strand, within MMJJ_RS07280at 1334.956 kb on + strandat 1335.045 kb on - strandat 1335.047 kb on - strandat 1335.047 kb on - strandat 1335.047 kb on - strandat 1335.048 kb on + strandat 1335.048 kb on + strandat 1335.048 kb on + strandat 1335.048 kb on + strandat 1335.051 kb on + strandat 1335.051 kb on + strandat 1335.051 kb on + strandat 1335.092 kb on - strandat 1335.095 kb on - strandat 1335.096 kb on + strandat 1335.098 kb on + strandat 1335.103 kb on + strandat 1335.103 kb on + strandat 1335.107 kb on + strandat 1335.135 kb on + strandat 1335.160 kb on - strandat 1335.160 kb on - strandat 1335.161 kb on + strandat 1335.163 kb on - strandat 1335.163 kb on - strandat 1335.164 kb on + strandat 1335.164 kb on + strandat 1335.167 kb on - strandat 1335.171 kb on + strandat 1335.171 kb on + strandat 1335.172 kb on - strandat 1335.173 kb on + strandat 1335.175 kb on - strandat 1335.178 kb on - strandat 1335.180 kb on - strandat 1335.181 kb on + strandat 1335.601 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM
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1,332,696 + MMJJ_RS07275 0.14 -1.2
1,332,735 - MMJJ_RS07275 0.18 -1.2
1,332,736 + MMJJ_RS07275 0.18 +1.1
1,333,205 + MMJJ_RS07275 0.61 +0.1
1,333,217 - MMJJ_RS07275 0.62 -0.9
1,333,324 - MMJJ_RS07275 0.72 +0.1
1,333,436 + MMJJ_RS07275 0.83 -0.5
1,333,436 + MMJJ_RS07275 0.83 -1.5
1,333,505 - MMJJ_RS07275 0.89 -0.5
1,333,617 + -0.5
1,333,623 - +3.8
1,333,624 + +0.5
1,333,624 + +1.9
1,333,659 + +0.9
1,333,673 - +1.1
1,333,679 - +0.1
1,333,698 - +0.4
1,333,719 - -0.9
1,333,815 + +0.1
1,334,002 - MMJJ_RS07280 0.24 -1.5
1,334,013 - MMJJ_RS07280 0.25 -2.0
1,334,050 - MMJJ_RS07280 0.27 -3.7
1,334,116 - MMJJ_RS07280 0.32 -1.9
1,334,116 - MMJJ_RS07280 0.32 -1.2
1,334,117 + MMJJ_RS07280 0.33 -2.1
1,334,136 - MMJJ_RS07280 0.34 +0.1
1,334,253 - MMJJ_RS07280 0.43 -2.2
1,334,254 + MMJJ_RS07280 0.43 -3.0
1,334,254 + MMJJ_RS07280 0.43 -0.5
1,334,358 - MMJJ_RS07280 0.51 -1.9
1,334,358 - MMJJ_RS07280 0.51 -0.8
1,334,385 + MMJJ_RS07280 0.53 -3.9
1,334,440 + MMJJ_RS07280 0.57 -1.9
1,334,444 - MMJJ_RS07280 0.57 +2.2
1,334,444 - MMJJ_RS07280 0.57 -0.2
1,334,568 - MMJJ_RS07280 0.67 -1.6
1,334,667 - MMJJ_RS07280 0.74 -2.1
1,334,716 - MMJJ_RS07280 0.78 -4.0
1,334,716 - MMJJ_RS07280 0.78 +0.8
1,334,782 - MMJJ_RS07280 0.83 -3.3
1,334,782 - MMJJ_RS07280 0.83 -2.7
1,334,782 - MMJJ_RS07280 0.83 -0.7
1,334,782 - MMJJ_RS07280 0.83 +0.1
1,334,783 + MMJJ_RS07280 0.83 -0.9
1,334,835 - MMJJ_RS07280 0.87 -3.5
1,334,847 + MMJJ_RS07280 0.88 -2.5
1,334,956 + -1.9
1,335,045 - -1.5
1,335,047 - -2.1
1,335,047 - -3.8
1,335,047 - +0.4
1,335,048 + -1.9
1,335,048 + -2.5
1,335,048 + -0.1
1,335,048 + -0.4
1,335,051 + +3.8
1,335,051 + -2.0
1,335,051 + -2.8
1,335,092 - -1.7
1,335,095 - +0.4
1,335,096 + -0.9
1,335,098 + -1.5
1,335,103 + +1.4
1,335,103 + -4.0
1,335,107 + -1.5
1,335,135 + +2.1
1,335,160 - +1.3
1,335,160 - -0.7
1,335,161 + +1.0
1,335,163 - +2.3
1,335,163 - -2.5
1,335,164 + -0.4
1,335,164 + +3.8
1,335,167 - +0.1
1,335,171 + +0.2
1,335,171 + -2.4
1,335,172 - +2.6
1,335,173 + -0.3
1,335,175 - +0.8
1,335,178 - -1.1
1,335,180 - -1.5
1,335,181 + -0.1
1,335,601 + -0.9

Or see this region's nucleotide sequence