Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05540 and MMJJ_RS05545 are separated by 74 nucleotides MMJJ_RS05545 and MMJJ_RS05550 are separated by 33 nucleotides MMJJ_RS05550 and MMJJ_RS05555 overlap by 44 nucleotides MMJJ_RS05555 and MMJJ_RS05560 are separated by 22 nucleotides
MMJJ_RS05540: MMJJ_RS05540 - LysR family transcriptional regulator, at 1,036,718 to 1,037,608
_RS05540
MMJJ_RS05545: MMJJ_RS05545 - DNA polymerase sliding clamp, at 1,037,683 to 1,038,435
_RS05545
MMJJ_RS05550: MMJJ_RS05550 - hypothetical protein, at 1,038,469 to 1,039,056
_RS05550
MMJJ_RS05555: MMJJ_RS05555 - 50S ribosomal protein L44e, at 1,039,013 to 1,039,294
_RS05555
MMJJ_RS05560: MMJJ_RS05560 - 30S ribosomal protein S27e, at 1,039,317 to 1,039,502
_RS05560
Position (kb)
1037
1038
1039 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1036.686 kb on - strand at 1036.686 kb on - strand at 1036.686 kb on - strand at 1036.688 kb on - strand at 1036.700 kb on - strand at 1036.706 kb on + strand at 1036.706 kb on + strand at 1036.706 kb on + strand at 1036.706 kb on + strand at 1036.706 kb on + strand at 1036.706 kb on + strand at 1036.736 kb on - strand at 1036.753 kb on + strand at 1036.768 kb on - strand at 1036.769 kb on + strand at 1036.781 kb on + strand at 1036.818 kb on - strand, within MMJJ_RS05540 at 1036.818 kb on - strand, within MMJJ_RS05540 at 1036.828 kb on - strand, within MMJJ_RS05540 at 1036.828 kb on - strand, within MMJJ_RS05540 at 1036.828 kb on - strand, within MMJJ_RS05540 at 1036.828 kb on - strand, within MMJJ_RS05540 at 1036.828 kb on - strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.829 kb on + strand, within MMJJ_RS05540 at 1036.830 kb on - strand, within MMJJ_RS05540 at 1036.830 kb on - strand, within MMJJ_RS05540 at 1036.830 kb on - strand, within MMJJ_RS05540 at 1036.831 kb on + strand, within MMJJ_RS05540 at 1036.893 kb on - strand, within MMJJ_RS05540 at 1036.894 kb on + strand, within MMJJ_RS05540 at 1036.894 kb on + strand, within MMJJ_RS05540 at 1036.945 kb on - strand, within MMJJ_RS05540 at 1036.946 kb on + strand, within MMJJ_RS05540 at 1036.947 kb on - strand, within MMJJ_RS05540 at 1036.947 kb on - strand, within MMJJ_RS05540 at 1036.947 kb on - strand, within MMJJ_RS05540 at 1036.951 kb on - strand, within MMJJ_RS05540 at 1036.951 kb on - strand, within MMJJ_RS05540 at 1036.951 kb on - strand, within MMJJ_RS05540 at 1036.952 kb on + strand, within MMJJ_RS05540 at 1036.998 kb on - strand, within MMJJ_RS05540 at 1037.017 kb on - strand, within MMJJ_RS05540 at 1037.017 kb on - strand, within MMJJ_RS05540 at 1037.018 kb on + strand, within MMJJ_RS05540 at 1037.018 kb on + strand, within MMJJ_RS05540 at 1037.018 kb on + strand, within MMJJ_RS05540 at 1037.037 kb on - strand, within MMJJ_RS05540 at 1037.037 kb on - strand, within MMJJ_RS05540 at 1037.038 kb on + strand, within MMJJ_RS05540 at 1037.038 kb on + strand, within MMJJ_RS05540 at 1037.038 kb on + strand, within MMJJ_RS05540 at 1037.038 kb on + strand, within MMJJ_RS05540 at 1037.038 kb on + strand, within MMJJ_RS05540 at 1037.041 kb on + strand, within MMJJ_RS05540 at 1037.041 kb on + strand, within MMJJ_RS05540 at 1037.047 kb on - strand, within MMJJ_RS05540 at 1037.048 kb on + strand, within MMJJ_RS05540 at 1037.049 kb on - strand, within MMJJ_RS05540 at 1037.049 kb on - strand, within MMJJ_RS05540 at 1037.049 kb on - strand, within MMJJ_RS05540 at 1037.049 kb on - strand, within MMJJ_RS05540 at 1037.050 kb on + strand, within MMJJ_RS05540 at 1037.050 kb on + strand, within MMJJ_RS05540 at 1037.053 kb on - strand, within MMJJ_RS05540 at 1037.053 kb on - strand, within MMJJ_RS05540 at 1037.053 kb on - strand, within MMJJ_RS05540 at 1037.060 kb on - strand, within MMJJ_RS05540 at 1037.060 kb on - strand, within MMJJ_RS05540 at 1037.061 kb on + strand, within MMJJ_RS05540 at 1037.062 kb on - strand, within MMJJ_RS05540 at 1037.062 kb on - strand, within MMJJ_RS05540 at 1037.062 kb on - strand, within MMJJ_RS05540 at 1037.062 kb on - strand, within MMJJ_RS05540 at 1037.063 kb on + strand, within MMJJ_RS05540 at 1037.063 kb on + strand, within MMJJ_RS05540 at 1037.063 kb on + strand, within MMJJ_RS05540 at 1037.072 kb on - strand, within MMJJ_RS05540 at 1037.101 kb on - strand, within MMJJ_RS05540 at 1037.151 kb on - strand, within MMJJ_RS05540 at 1037.151 kb on - strand, within MMJJ_RS05540 at 1037.151 kb on - strand, within MMJJ_RS05540 at 1037.151 kb on - strand, within MMJJ_RS05540 at 1037.152 kb on + strand, within MMJJ_RS05540 at 1037.152 kb on + strand, within MMJJ_RS05540 at 1037.152 kb on + strand, within MMJJ_RS05540 at 1037.167 kb on - strand, within MMJJ_RS05540 at 1037.167 kb on - strand, within MMJJ_RS05540 at 1037.184 kb on + strand, within MMJJ_RS05540 at 1037.197 kb on + strand, within MMJJ_RS05540 at 1037.210 kb on + strand, within MMJJ_RS05540 at 1037.218 kb on + strand, within MMJJ_RS05540 at 1037.223 kb on - strand, within MMJJ_RS05540 at 1037.223 kb on - strand, within MMJJ_RS05540 at 1037.223 kb on - strand, within MMJJ_RS05540 at 1037.223 kb on - strand, within MMJJ_RS05540 at 1037.224 kb on + strand, within MMJJ_RS05540 at 1037.224 kb on + strand, within MMJJ_RS05540 at 1037.275 kb on - strand, within MMJJ_RS05540 at 1037.275 kb on - strand, within MMJJ_RS05540 at 1037.275 kb on - strand at 1037.276 kb on + strand, within MMJJ_RS05540 at 1037.277 kb on - strand, within MMJJ_RS05540 at 1037.277 kb on - strand, within MMJJ_RS05540 at 1037.278 kb on + strand, within MMJJ_RS05540 at 1037.278 kb on + strand, within MMJJ_RS05540 at 1037.280 kb on - strand, within MMJJ_RS05540 at 1037.281 kb on + strand, within MMJJ_RS05540 at 1037.291 kb on - strand, within MMJJ_RS05540 at 1037.291 kb on - strand, within MMJJ_RS05540 at 1037.291 kb on - strand, within MMJJ_RS05540 at 1037.292 kb on + strand, within MMJJ_RS05540 at 1037.328 kb on - strand, within MMJJ_RS05540 at 1037.328 kb on - strand, within MMJJ_RS05540 at 1037.329 kb on + strand, within MMJJ_RS05540 at 1037.329 kb on + strand, within MMJJ_RS05540 at 1037.437 kb on - strand, within MMJJ_RS05540 at 1037.437 kb on - strand, within MMJJ_RS05540 at 1037.438 kb on + strand, within MMJJ_RS05540 at 1037.438 kb on + strand, within MMJJ_RS05540 at 1037.446 kb on - strand, within MMJJ_RS05540 at 1037.515 kb on + strand, within MMJJ_RS05540 at 1037.534 kb on - strand at 1037.534 kb on - strand at 1037.534 kb on - strand at 1037.535 kb on + strand at 1037.541 kb on - strand at 1037.575 kb on + strand at 1037.621 kb on - strand at 1037.621 kb on - strand at 1037.631 kb on - strand at 1037.631 kb on - strand at 1037.631 kb on - strand at 1037.631 kb on - strand at 1037.632 kb on + strand at 1037.632 kb on + strand at 1037.632 kb on + strand at 1037.632 kb on + strand at 1037.632 kb on + strand at 1037.633 kb on - strand at 1037.634 kb on + strand at 1037.634 kb on + strand at 1037.636 kb on + strand at 1037.701 kb on + strand at 1037.747 kb on - strand at 1038.473 kb on + strand at 1038.482 kb on - strand at 1039.292 kb on - strand at 1039.292 kb on - strand at 1039.293 kb on + strand at 1039.325 kb on + strand at 1039.337 kb on - strand, within MMJJ_RS05560 at 1039.338 kb on + strand, within MMJJ_RS05560 at 1039.388 kb on - strand, within MMJJ_RS05560 at 1039.388 kb on - strand, within MMJJ_RS05560 at 1039.389 kb on + strand, within MMJJ_RS05560 at 1039.416 kb on + strand, within MMJJ_RS05560
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 1,036,686 - +1.7 1,036,686 - -0.6 1,036,686 - +0.4 1,036,688 - +2.2 1,036,700 - +0.5 1,036,706 + +0.7 1,036,706 + +1.5 1,036,706 + -1.3 1,036,706 + +1.0 1,036,706 + +0.1 1,036,706 + -0.1 1,036,736 - +0.9 1,036,753 + +1.6 1,036,768 - -0.2 1,036,769 + -0.5 1,036,781 + -0.5 1,036,818 - MMJJ_RS05540 0.11 +0.7 1,036,818 - MMJJ_RS05540 0.11 +1.1 1,036,828 - MMJJ_RS05540 0.12 +1.7 1,036,828 - MMJJ_RS05540 0.12 +0.6 1,036,828 - MMJJ_RS05540 0.12 +2.7 1,036,828 - MMJJ_RS05540 0.12 -0.4 1,036,828 - MMJJ_RS05540 0.12 -0.5 1,036,829 + MMJJ_RS05540 0.12 -0.5 1,036,829 + MMJJ_RS05540 0.12 +2.1 1,036,829 + MMJJ_RS05540 0.12 -0.5 1,036,829 + MMJJ_RS05540 0.12 +0.1 1,036,829 + MMJJ_RS05540 0.12 -2.1 1,036,829 + MMJJ_RS05540 0.12 -0.9 1,036,829 + MMJJ_RS05540 0.12 +0.7 1,036,829 + MMJJ_RS05540 0.12 +1.4 1,036,829 + MMJJ_RS05540 0.12 +0.1 1,036,829 + MMJJ_RS05540 0.12 +4.3 1,036,830 - MMJJ_RS05540 0.13 +1.6 1,036,830 - MMJJ_RS05540 0.13 -2.5 1,036,830 - MMJJ_RS05540 0.13 +2.6 1,036,831 + MMJJ_RS05540 0.13 -2.0 1,036,893 - MMJJ_RS05540 0.20 -1.9 1,036,894 + MMJJ_RS05540 0.20 +1.3 1,036,894 + MMJJ_RS05540 0.20 +0.1 1,036,945 - MMJJ_RS05540 0.25 +0.4 1,036,946 + MMJJ_RS05540 0.26 -0.9 1,036,947 - MMJJ_RS05540 0.26 +0.4 1,036,947 - MMJJ_RS05540 0.26 +4.0 1,036,947 - MMJJ_RS05540 0.26 -0.2 1,036,951 - MMJJ_RS05540 0.26 +1.2 1,036,951 - MMJJ_RS05540 0.26 -0.5 1,036,951 - MMJJ_RS05540 0.26 +1.4 1,036,952 + MMJJ_RS05540 0.26 +1.0 1,036,998 - MMJJ_RS05540 0.31 +2.7 1,037,017 - MMJJ_RS05540 0.34 +1.9 1,037,017 - MMJJ_RS05540 0.34 +1.4 1,037,018 + MMJJ_RS05540 0.34 +0.3 1,037,018 + MMJJ_RS05540 0.34 +1.1 1,037,018 + MMJJ_RS05540 0.34 +0.7 1,037,037 - MMJJ_RS05540 0.36 -0.3 1,037,037 - MMJJ_RS05540 0.36 +2.3 1,037,038 + MMJJ_RS05540 0.36 +1.1 1,037,038 + MMJJ_RS05540 0.36 +0.7 1,037,038 + MMJJ_RS05540 0.36 +0.1 1,037,038 + MMJJ_RS05540 0.36 -2.4 1,037,038 + MMJJ_RS05540 0.36 -1.3 1,037,041 + MMJJ_RS05540 0.36 +0.1 1,037,041 + MMJJ_RS05540 0.36 +1.2 1,037,047 - MMJJ_RS05540 0.37 +0.1 1,037,048 + MMJJ_RS05540 0.37 -0.1 1,037,049 - MMJJ_RS05540 0.37 +0.1 1,037,049 - MMJJ_RS05540 0.37 +1.1 1,037,049 - MMJJ_RS05540 0.37 +1.2 1,037,049 - MMJJ_RS05540 0.37 +1.1 1,037,050 + MMJJ_RS05540 0.37 +0.6 1,037,050 + MMJJ_RS05540 0.37 +0.2 1,037,053 - MMJJ_RS05540 0.38 +1.4 1,037,053 - MMJJ_RS05540 0.38 +2.6 1,037,053 - MMJJ_RS05540 0.38 -0.9 1,037,060 - MMJJ_RS05540 0.38 +1.4 1,037,060 - MMJJ_RS05540 0.38 +1.1 1,037,061 + MMJJ_RS05540 0.38 +0.5 1,037,062 - MMJJ_RS05540 0.39 +2.4 1,037,062 - MMJJ_RS05540 0.39 -0.8 1,037,062 - MMJJ_RS05540 0.39 +0.9 1,037,062 - MMJJ_RS05540 0.39 +1.1 1,037,063 + MMJJ_RS05540 0.39 +1.1 1,037,063 + MMJJ_RS05540 0.39 +0.1 1,037,063 + MMJJ_RS05540 0.39 -1.3 1,037,072 - MMJJ_RS05540 0.40 +0.1 1,037,101 - MMJJ_RS05540 0.43 +1.7 1,037,151 - MMJJ_RS05540 0.49 -1.3 1,037,151 - MMJJ_RS05540 0.49 +0.6 1,037,151 - MMJJ_RS05540 0.49 +0.6 1,037,151 - MMJJ_RS05540 0.49 +1.7 1,037,152 + MMJJ_RS05540 0.49 +0.3 1,037,152 + MMJJ_RS05540 0.49 -1.3 1,037,152 + MMJJ_RS05540 0.49 +1.2 1,037,167 - MMJJ_RS05540 0.50 -0.4 1,037,167 - MMJJ_RS05540 0.50 +1.7 1,037,184 + MMJJ_RS05540 0.52 +0.3 1,037,197 + MMJJ_RS05540 0.54 +0.7 1,037,210 + MMJJ_RS05540 0.55 +1.1 1,037,218 + MMJJ_RS05540 0.56 -1.3 1,037,223 - MMJJ_RS05540 0.57 +0.3 1,037,223 - MMJJ_RS05540 0.57 -0.5 1,037,223 - MMJJ_RS05540 0.57 -0.7 1,037,223 - MMJJ_RS05540 0.57 -2.1 1,037,224 + MMJJ_RS05540 0.57 +0.7 1,037,224 + MMJJ_RS05540 0.57 +1.5 1,037,275 - MMJJ_RS05540 0.63 -0.9 1,037,275 - MMJJ_RS05540 0.63 +3.4 1,037,275 - -0.9 1,037,276 + MMJJ_RS05540 0.63 -2.1 1,037,277 - MMJJ_RS05540 0.63 +1.4 1,037,277 - MMJJ_RS05540 0.63 +0.4 1,037,278 + MMJJ_RS05540 0.63 +1.3 1,037,278 + MMJJ_RS05540 0.63 +1.8 1,037,280 - MMJJ_RS05540 0.63 -0.3 1,037,281 + MMJJ_RS05540 0.63 +0.4 1,037,291 - MMJJ_RS05540 0.64 -2.1 1,037,291 - MMJJ_RS05540 0.64 +0.3 1,037,291 - MMJJ_RS05540 0.64 +0.7 1,037,292 + MMJJ_RS05540 0.64 -0.7 1,037,328 - MMJJ_RS05540 0.68 -0.0 1,037,328 - MMJJ_RS05540 0.68 -0.9 1,037,329 + MMJJ_RS05540 0.69 +0.7 1,037,329 + MMJJ_RS05540 0.69 +1.8 1,037,437 - MMJJ_RS05540 0.81 -0.7 1,037,437 - MMJJ_RS05540 0.81 +2.9 1,037,438 + MMJJ_RS05540 0.81 -0.5 1,037,438 + MMJJ_RS05540 0.81 -2.3 1,037,446 - MMJJ_RS05540 0.82 +1.3 1,037,515 + MMJJ_RS05540 0.89 -0.3 1,037,534 - +0.6 1,037,534 - +1.2 1,037,534 - +2.2 1,037,535 + -0.5 1,037,541 - -2.1 1,037,575 + +1.3 1,037,621 - +0.3 1,037,621 - +1.4 1,037,631 - -0.3 1,037,631 - -0.9 1,037,631 - -1.5 1,037,631 - -1.9 1,037,632 + -2.5 1,037,632 + +0.6 1,037,632 + -3.5 1,037,632 + -2.8 1,037,632 + -3.2 1,037,633 - +0.1 1,037,634 + +0.1 1,037,634 + +1.1 1,037,636 + -0.3 1,037,701 + -0.5 1,037,747 - -0.5 1,038,473 + +1.7 1,038,482 - -1.9 1,039,292 - -0.9 1,039,292 - -0.5 1,039,293 + -0.9 1,039,325 + +0.4 1,039,337 - MMJJ_RS05560 0.11 -1.4 1,039,338 + MMJJ_RS05560 0.11 -2.4 1,039,388 - MMJJ_RS05560 0.38 -3.8 1,039,388 - MMJJ_RS05560 0.38 -2.4 1,039,389 + MMJJ_RS05560 0.39 -1.8 1,039,416 + MMJJ_RS05560 0.53 -1.3
Or see this region's nucleotide sequence