Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04740 and MMJJ_RS04745 are separated by 77 nucleotides MMJJ_RS04745 and MMJJ_RS04750 are separated by 305 nucleotides MMJJ_RS04750 and MMJJ_RS04755 are separated by 59 nucleotides
MMJJ_RS04740: MMJJ_RS04740 - histidinol phosphate phosphatase domain-containing protein, at 889,321 to 889,977
_RS04740
MMJJ_RS04745: MMJJ_RS04745 - cyclase family protein, at 890,055 to 890,624
_RS04745
MMJJ_RS04750: MMJJ_RS04750 - 5,10-methenyltetrahydromethanopterin hydrogenase, at 890,930 to 891,994
_RS04750
MMJJ_RS04755: MMJJ_RS04755 - 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB, at 892,054 to 893,079
_RS04755
Position (kb) 890
891
892 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 890.014 kb on - strand at 890.015 kb on + strand at 890.015 kb on + strand at 890.016 kb on - strand at 890.016 kb on - strand at 890.017 kb on + strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.021 kb on + strand at 890.023 kb on - strand at 890.026 kb on - strand at 890.026 kb on - strand at 890.031 kb on + strand at 890.034 kb on - strand at 890.034 kb on - strand at 890.042 kb on + strand at 890.071 kb on - strand at 890.072 kb on + strand at 890.104 kb on - strand at 890.104 kb on - strand at 890.155 kb on + strand, within MMJJ_RS04745 at 890.226 kb on + strand, within MMJJ_RS04745 at 890.268 kb on + strand, within MMJJ_RS04745 at 890.353 kb on + strand, within MMJJ_RS04745 at 890.363 kb on - strand, within MMJJ_RS04745 at 890.364 kb on + strand, within MMJJ_RS04745 at 890.364 kb on + strand, within MMJJ_RS04745 at 890.370 kb on - strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.414 kb on + strand, within MMJJ_RS04745 at 890.421 kb on + strand, within MMJJ_RS04745 at 890.489 kb on + strand, within MMJJ_RS04745 at 890.507 kb on + strand, within MMJJ_RS04745 at 890.516 kb on - strand, within MMJJ_RS04745 at 890.517 kb on + strand, within MMJJ_RS04745 at 890.604 kb on + strand at 890.643 kb on + strand at 890.668 kb on + strand at 890.710 kb on + strand at 890.713 kb on + strand at 890.726 kb on - strand at 890.756 kb on + strand at 890.763 kb on + strand at 890.765 kb on - strand at 890.811 kb on + strand at 890.813 kb on + strand at 890.816 kb on - strand at 890.822 kb on - strand at 890.858 kb on - strand at 890.858 kb on - strand at 890.859 kb on + strand at 890.886 kb on - strand at 890.887 kb on + strand at 890.887 kb on + strand at 890.895 kb on - strand at 890.896 kb on + strand at 890.901 kb on + strand at 890.901 kb on + strand at 890.904 kb on + strand at 890.905 kb on - strand at 890.905 kb on - strand at 890.905 kb on - strand at 890.906 kb on + strand at 890.906 kb on + strand at 890.906 kb on + strand at 890.910 kb on - strand at 890.910 kb on - strand at 890.910 kb on - strand at 890.914 kb on - strand at 890.915 kb on + strand at 890.924 kb on - strand at 890.924 kb on - strand at 890.925 kb on + strand at 890.925 kb on + strand at 890.930 kb on + strand at 890.943 kb on - strand at 890.947 kb on + strand at 890.947 kb on + strand at 890.947 kb on + strand at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.088 kb on + strand, within MMJJ_RS04750 at 891.100 kb on - strand, within MMJJ_RS04750 at 891.151 kb on + strand, within MMJJ_RS04750 at 891.153 kb on - strand, within MMJJ_RS04750 at 891.154 kb on + strand, within MMJJ_RS04750 at 891.182 kb on + strand, within MMJJ_RS04750 at 891.196 kb on + strand, within MMJJ_RS04750 at 891.241 kb on + strand, within MMJJ_RS04750 at 891.253 kb on - strand, within MMJJ_RS04750 at 891.295 kb on + strand, within MMJJ_RS04750 at 891.295 kb on + strand, within MMJJ_RS04750 at 891.364 kb on + strand, within MMJJ_RS04750 at 891.367 kb on - strand, within MMJJ_RS04750 at 891.367 kb on - strand, within MMJJ_RS04750 at 891.368 kb on + strand, within MMJJ_RS04750 at 891.368 kb on + strand, within MMJJ_RS04750 at 891.402 kb on - strand, within MMJJ_RS04750 at 891.445 kb on + strand, within MMJJ_RS04750 at 891.458 kb on + strand, within MMJJ_RS04750 at 891.514 kb on + strand, within MMJJ_RS04750 at 891.527 kb on - strand, within MMJJ_RS04750 at 891.583 kb on + strand, within MMJJ_RS04750 at 891.611 kb on - strand, within MMJJ_RS04750 at 891.611 kb on - strand, within MMJJ_RS04750 at 891.616 kb on - strand, within MMJJ_RS04750 at 891.663 kb on - strand, within MMJJ_RS04750 at 891.697 kb on - strand, within MMJJ_RS04750 at 891.754 kb on + strand, within MMJJ_RS04750 at 891.862 kb on - strand, within MMJJ_RS04750 at 891.913 kb on - strand at 891.914 kb on + strand at 891.914 kb on + strand at 891.992 kb on - strand at 891.997 kb on + strand at 891.997 kb on + strand at 892.000 kb on + strand at 892.002 kb on - strand at 892.003 kb on + strand at 892.009 kb on - strand at 892.010 kb on + strand at 892.038 kb on + strand at 892.059 kb on + strand at 892.070 kb on - strand at 892.107 kb on + strand at 892.112 kb on - strand at 892.112 kb on - strand at 892.125 kb on + strand at 892.152 kb on + strand at 892.155 kb on - strand at 892.156 kb on + strand at 892.181 kb on - strand, within MMJJ_RS04755 at 892.182 kb on + strand, within MMJJ_RS04755 at 892.210 kb on + strand at 892.210 kb on + strand, within MMJJ_RS04755 at 892.256 kb on - strand, within MMJJ_RS04755 at 892.256 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.264 kb on + strand, within MMJJ_RS04755 at 892.291 kb on - strand, within MMJJ_RS04755 at 892.292 kb on + strand, within MMJJ_RS04755 at 892.328 kb on + strand, within MMJJ_RS04755 at 892.331 kb on - strand, within MMJJ_RS04755 at 892.331 kb on - strand, within MMJJ_RS04755 at 892.332 kb on + strand, within MMJJ_RS04755 at 892.332 kb on + strand, within MMJJ_RS04755 at 892.445 kb on - strand, within MMJJ_RS04755 at 892.457 kb on - strand, within MMJJ_RS04755 at 892.484 kb on - strand, within MMJJ_RS04755 at 892.514 kb on - strand, within MMJJ_RS04755 at 892.546 kb on - strand, within MMJJ_RS04755 at 892.548 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand at 892.557 kb on - strand, within MMJJ_RS04755 at 892.558 kb on + strand, within MMJJ_RS04755 at 892.558 kb on + strand, within MMJJ_RS04755 at 892.563 kb on + strand, within MMJJ_RS04755 at 892.637 kb on - strand, within MMJJ_RS04755 at 892.671 kb on + strand, within MMJJ_RS04755 at 892.676 kb on - strand, within MMJJ_RS04755 at 892.694 kb on - strand, within MMJJ_RS04755 at 892.699 kb on + strand, within MMJJ_RS04755 at 892.719 kb on - strand, within MMJJ_RS04755 at 892.720 kb on + strand, within MMJJ_RS04755 at 892.773 kb on + strand, within MMJJ_RS04755 at 892.790 kb on + strand, within MMJJ_RS04755 at 892.860 kb on + strand, within MMJJ_RS04755 at 892.888 kb on + strand, within MMJJ_RS04755 at 892.964 kb on - strand, within MMJJ_RS04755 at 892.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 890,014 - +1.0 890,015 + -2.5 890,015 + +1.4 890,016 - +1.8 890,016 - -0.8 890,017 + -1.0 890,020 - -0.5 890,020 - -2.2 890,020 - -1.8 890,020 - +2.6 890,020 - +1.5 890,020 - -1.8 890,021 + -2.6 890,023 - +1.0 890,026 - -0.6 890,026 - -0.6 890,031 + -2.7 890,034 - +2.3 890,034 - -1.1 890,042 + +2.0 890,071 - -2.5 890,072 + +1.3 890,104 - -3.1 890,104 - -2.5 890,155 + MMJJ_RS04745 0.18 -2.0 890,226 + MMJJ_RS04745 0.30 -0.9 890,268 + MMJJ_RS04745 0.37 -0.1 890,353 + MMJJ_RS04745 0.52 -0.0 890,363 - MMJJ_RS04745 0.54 +0.6 890,364 + MMJJ_RS04745 0.54 +0.8 890,364 + MMJJ_RS04745 0.54 +1.2 890,370 - MMJJ_RS04745 0.55 -2.0 890,371 + MMJJ_RS04745 0.55 -0.3 890,371 + MMJJ_RS04745 0.55 +0.7 890,371 + MMJJ_RS04745 0.55 -2.3 890,414 + MMJJ_RS04745 0.63 +1.5 890,421 + MMJJ_RS04745 0.64 -1.0 890,489 + MMJJ_RS04745 0.76 +2.7 890,507 + MMJJ_RS04745 0.79 +0.2 890,516 - MMJJ_RS04745 0.81 +1.0 890,517 + MMJJ_RS04745 0.81 +0.9 890,604 + -0.2 890,643 + -2.6 890,668 + -1.9 890,710 + +0.4 890,713 + +1.1 890,726 - +0.7 890,756 + +0.4 890,763 + -0.6 890,765 - -0.3 890,811 + -1.8 890,813 + -1.5 890,816 - -2.8 890,822 - +0.5 890,858 - -3.2 890,858 - -4.0 890,859 + -1.1 890,886 - -0.9 890,887 + -2.1 890,887 + -2.5 890,895 - -0.5 890,896 + -3.0 890,901 + -1.5 890,901 + -3.5 890,904 + -3.3 890,905 - -1.6 890,905 - -3.1 890,905 - -3.2 890,906 + -1.9 890,906 + +0.7 890,906 + -0.3 890,910 - -1.4 890,910 - -3.6 890,910 - -3.3 890,914 - -2.5 890,915 + -1.5 890,924 - -2.5 890,924 - -4.0 890,925 + -2.7 890,925 + -1.8 890,930 + -3.7 890,943 - +0.2 890,947 + -1.2 890,947 + -1.0 890,947 + -0.6 891,087 - MMJJ_RS04750 0.15 -1.5 891,087 - MMJJ_RS04750 0.15 -3.0 891,087 - MMJJ_RS04750 0.15 -1.8 891,087 - MMJJ_RS04750 0.15 -2.7 891,088 + MMJJ_RS04750 0.15 -3.5 891,100 - MMJJ_RS04750 0.16 -2.0 891,151 + MMJJ_RS04750 0.21 -3.0 891,153 - MMJJ_RS04750 0.21 -3.2 891,154 + MMJJ_RS04750 0.21 -3.3 891,182 + MMJJ_RS04750 0.24 -3.1 891,196 + MMJJ_RS04750 0.25 -2.6 891,241 + MMJJ_RS04750 0.29 -2.3 891,253 - MMJJ_RS04750 0.30 -2.3 891,295 + MMJJ_RS04750 0.34 -0.5 891,295 + MMJJ_RS04750 0.34 -3.7 891,364 + MMJJ_RS04750 0.41 -2.9 891,367 - MMJJ_RS04750 0.41 -0.7 891,367 - MMJJ_RS04750 0.41 -1.3 891,368 + MMJJ_RS04750 0.41 -2.0 891,368 + MMJJ_RS04750 0.41 -3.2 891,402 - MMJJ_RS04750 0.44 -3.1 891,445 + MMJJ_RS04750 0.48 -0.6 891,458 + MMJJ_RS04750 0.50 -2.0 891,514 + MMJJ_RS04750 0.55 -3.9 891,527 - MMJJ_RS04750 0.56 -3.0 891,583 + MMJJ_RS04750 0.61 -1.0 891,611 - MMJJ_RS04750 0.64 -1.1 891,611 - MMJJ_RS04750 0.64 -3.6 891,616 - MMJJ_RS04750 0.64 -0.6 891,663 - MMJJ_RS04750 0.69 -0.0 891,697 - MMJJ_RS04750 0.72 -1.2 891,754 + MMJJ_RS04750 0.77 -1.0 891,862 - MMJJ_RS04750 0.88 -1.3 891,913 - -2.1 891,914 + -3.3 891,914 + -3.1 891,992 - -1.6 891,997 + -1.5 891,997 + -1.8 892,000 + -3.0 892,002 - -1.0 892,003 + -2.9 892,009 - -2.2 892,010 + -4.3 892,038 + -1.0 892,059 + -0.9 892,070 - -3.7 892,107 + -2.8 892,112 - -3.3 892,112 - -2.6 892,125 + -2.0 892,152 + -4.6 892,155 - -2.3 892,156 + -2.9 892,181 - MMJJ_RS04755 0.12 -2.0 892,182 + MMJJ_RS04755 0.12 -0.7 892,210 + -1.0 892,210 + MMJJ_RS04755 0.15 -2.1 892,256 - MMJJ_RS04755 0.20 -1.9 892,256 - MMJJ_RS04755 0.20 -1.6 892,260 - MMJJ_RS04755 0.20 -1.8 892,260 - MMJJ_RS04755 0.20 -1.6 892,260 - MMJJ_RS04755 0.20 -2.2 892,260 - MMJJ_RS04755 0.20 +0.5 892,261 + MMJJ_RS04755 0.20 -2.8 892,261 + MMJJ_RS04755 0.20 -1.0 892,261 + MMJJ_RS04755 0.20 -3.7 892,264 + MMJJ_RS04755 0.20 -4.1 892,291 - MMJJ_RS04755 0.23 -1.3 892,292 + MMJJ_RS04755 0.23 -1.2 892,328 + MMJJ_RS04755 0.27 -3.4 892,331 - MMJJ_RS04755 0.27 -2.2 892,331 - MMJJ_RS04755 0.27 -1.8 892,332 + MMJJ_RS04755 0.27 +0.2 892,332 + MMJJ_RS04755 0.27 -3.3 892,445 - MMJJ_RS04755 0.38 -0.5 892,457 - MMJJ_RS04755 0.39 +0.6 892,484 - MMJJ_RS04755 0.42 -3.5 892,514 - MMJJ_RS04755 0.45 -3.5 892,546 - MMJJ_RS04755 0.48 -1.6 892,548 - MMJJ_RS04755 0.48 -0.6 892,553 - MMJJ_RS04755 0.49 -1.6 892,553 - MMJJ_RS04755 0.49 -2.6 892,553 - +1.0 892,557 - MMJJ_RS04755 0.49 -3.5 892,558 + MMJJ_RS04755 0.49 -3.2 892,558 + MMJJ_RS04755 0.49 -1.7 892,563 + MMJJ_RS04755 0.50 -2.2 892,637 - MMJJ_RS04755 0.57 -0.6 892,671 + MMJJ_RS04755 0.60 -2.7 892,676 - MMJJ_RS04755 0.61 -3.1 892,694 - MMJJ_RS04755 0.62 -2.2 892,699 + MMJJ_RS04755 0.63 -3.7 892,719 - MMJJ_RS04755 0.65 -3.0 892,720 + MMJJ_RS04755 0.65 -3.5 892,773 + MMJJ_RS04755 0.70 -3.3 892,790 + MMJJ_RS04755 0.72 -4.1 892,860 + MMJJ_RS04755 0.79 -0.0 892,888 + MMJJ_RS04755 0.81 -2.6 892,964 - MMJJ_RS04755 0.89 -2.6 892,992 - -1.9
Or see this region's nucleotide sequence