Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03715 and MMJJ_RS03720 are separated by 123 nucleotides MMJJ_RS03720 and MMJJ_RS03725 are separated by 9 nucleotides MMJJ_RS03725 and MMJJ_RS03730 are separated by 156 nucleotides MMJJ_RS03730 and MMJJ_RS03735 are separated by 72 nucleotides
MMJJ_RS03715: MMJJ_RS03715 - hypothetical protein, at 694,384 to 694,968
_RS03715
MMJJ_RS03720: MMJJ_RS03720 - DsrE family protein, at 695,092 to 695,436
_RS03720
MMJJ_RS03725: MMJJ_RS03725 - sulfurtransferase TusA family protein, at 695,446 to 695,658
_RS03725
MMJJ_RS03730: MMJJ_RS03730 - SWIM zinc finger family protein, at 695,815 to 696,504
_RS03730
MMJJ_RS03735: MMJJ_RS03735 - 2-oxoacid:acceptor oxidoreductase subunit alpha, at 696,577 to 698,289
_RS03735
Position (kb) 695
696
697 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 694.816 kb on - strand, within MMJJ_RS03715 at 694.817 kb on + strand at 694.839 kb on - strand, within MMJJ_RS03715 at 694.850 kb on - strand, within MMJJ_RS03715 at 694.850 kb on - strand, within MMJJ_RS03715 at 694.850 kb on - strand, within MMJJ_RS03715 at 694.851 kb on + strand, within MMJJ_RS03715 at 694.851 kb on + strand at 694.851 kb on + strand, within MMJJ_RS03715 at 694.860 kb on - strand, within MMJJ_RS03715 at 694.870 kb on - strand, within MMJJ_RS03715 at 694.875 kb on - strand, within MMJJ_RS03715 at 694.875 kb on - strand, within MMJJ_RS03715 at 694.876 kb on + strand, within MMJJ_RS03715 at 694.886 kb on - strand, within MMJJ_RS03715 at 694.887 kb on + strand, within MMJJ_RS03715 at 694.890 kb on - strand, within MMJJ_RS03715 at 694.891 kb on + strand, within MMJJ_RS03715 at 694.940 kb on + strand at 694.963 kb on + strand at 694.968 kb on - strand at 694.969 kb on + strand at 694.969 kb on + strand at 694.984 kb on - strand at 694.985 kb on + strand at 694.990 kb on + strand at 694.994 kb on - strand at 695.013 kb on - strand at 695.027 kb on - strand at 695.033 kb on - strand at 695.033 kb on - strand at 695.033 kb on - strand at 695.059 kb on - strand at 695.060 kb on + strand at 695.101 kb on + strand at 695.122 kb on - strand at 695.123 kb on + strand at 695.131 kb on - strand, within MMJJ_RS03720 at 695.132 kb on + strand, within MMJJ_RS03720 at 695.134 kb on - strand, within MMJJ_RS03720 at 695.135 kb on + strand, within MMJJ_RS03720 at 695.221 kb on - strand, within MMJJ_RS03720 at 695.222 kb on + strand, within MMJJ_RS03720 at 695.264 kb on - strand, within MMJJ_RS03720 at 695.265 kb on + strand, within MMJJ_RS03720 at 695.306 kb on - strand, within MMJJ_RS03720 at 695.307 kb on + strand, within MMJJ_RS03720 at 695.307 kb on + strand, within MMJJ_RS03720 at 695.329 kb on - strand, within MMJJ_RS03720 at 695.330 kb on + strand, within MMJJ_RS03720 at 695.330 kb on + strand, within MMJJ_RS03720 at 695.331 kb on - strand, within MMJJ_RS03720 at 695.362 kb on - strand, within MMJJ_RS03720 at 695.362 kb on - strand, within MMJJ_RS03720 at 695.374 kb on - strand, within MMJJ_RS03720 at 695.416 kb on - strand at 695.419 kb on - strand at 695.419 kb on - strand at 695.420 kb on + strand at 695.420 kb on + strand at 695.421 kb on - strand at 695.457 kb on - strand at 695.458 kb on + strand at 695.458 kb on + strand at 695.462 kb on + strand at 695.462 kb on + strand at 695.498 kb on + strand, within MMJJ_RS03725 at 695.511 kb on - strand, within MMJJ_RS03725 at 695.511 kb on - strand, within MMJJ_RS03725 at 695.632 kb on - strand, within MMJJ_RS03725 at 695.681 kb on - strand at 695.687 kb on - strand at 695.732 kb on + strand at 695.747 kb on + strand at 695.755 kb on + strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.768 kb on + strand at 695.771 kb on - strand at 695.784 kb on - strand at 695.837 kb on - strand at 695.838 kb on + strand at 695.838 kb on + strand at 695.844 kb on + strand at 695.863 kb on - strand at 695.864 kb on + strand at 695.864 kb on + strand at 695.883 kb on + strand at 695.917 kb on + strand, within MMJJ_RS03730 at 695.956 kb on - strand, within MMJJ_RS03730 at 695.968 kb on - strand, within MMJJ_RS03730 at 695.999 kb on - strand, within MMJJ_RS03730 at 696.000 kb on + strand, within MMJJ_RS03730 at 696.000 kb on + strand, within MMJJ_RS03730 at 696.000 kb on + strand, within MMJJ_RS03730 at 696.085 kb on + strand, within MMJJ_RS03730 at 696.229 kb on - strand, within MMJJ_RS03730 at 696.321 kb on + strand, within MMJJ_RS03730 at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.528 kb on - strand at 696.528 kb on - strand at 696.528 kb on - strand at 696.530 kb on - strand at 696.532 kb on - strand at 696.532 kb on - strand at 696.532 kb on - strand at 696.533 kb on + strand at 696.533 kb on + strand at 696.533 kb on + strand at 696.533 kb on + strand at 696.534 kb on - strand at 696.534 kb on - strand at 696.535 kb on + strand at 696.550 kb on - strand at 696.550 kb on - strand at 696.550 kb on - strand at 696.550 kb on - strand at 696.551 kb on + strand at 696.551 kb on + strand at 696.551 kb on + strand at 696.552 kb on - strand at 696.553 kb on + strand at 696.553 kb on + strand at 696.558 kb on - strand at 696.558 kb on - strand at 696.558 kb on - strand at 696.576 kb on - strand at 696.577 kb on + strand at 696.632 kb on - strand at 696.663 kb on + strand at 696.679 kb on - strand at 696.679 kb on - strand at 696.680 kb on + strand at 696.680 kb on + strand at 696.686 kb on - strand at 696.686 kb on - strand at 696.686 kb on - strand at 696.687 kb on + strand at 696.694 kb on + strand at 696.703 kb on - strand at 696.704 kb on + strand at 696.704 kb on + strand at 696.704 kb on + strand at 696.746 kb on - strand at 696.746 kb on - strand at 696.849 kb on - strand, within MMJJ_RS03735 at 696.876 kb on - strand, within MMJJ_RS03735 at 696.932 kb on - strand, within MMJJ_RS03735 at 696.933 kb on + strand, within MMJJ_RS03735 at 696.933 kb on + strand, within MMJJ_RS03735 at 696.987 kb on + strand, within MMJJ_RS03735 at 697.080 kb on + strand, within MMJJ_RS03735 at 697.100 kb on + strand at 697.118 kb on - strand, within MMJJ_RS03735 at 697.137 kb on + strand, within MMJJ_RS03735 at 697.137 kb on + strand, within MMJJ_RS03735 at 697.184 kb on - strand, within MMJJ_RS03735 at 697.191 kb on - strand, within MMJJ_RS03735 at 697.230 kb on - strand, within MMJJ_RS03735 at 697.230 kb on - strand, within MMJJ_RS03735 at 697.231 kb on + strand, within MMJJ_RS03735 at 697.311 kb on - strand, within MMJJ_RS03735 at 697.311 kb on - strand, within MMJJ_RS03735 at 697.316 kb on - strand, within MMJJ_RS03735 at 697.317 kb on + strand, within MMJJ_RS03735 at 697.350 kb on - strand, within MMJJ_RS03735 at 697.350 kb on - strand, within MMJJ_RS03735 at 697.392 kb on - strand, within MMJJ_RS03735 at 697.392 kb on - strand, within MMJJ_RS03735 at 697.396 kb on + strand, within MMJJ_RS03735 at 697.470 kb on + strand, within MMJJ_RS03735 at 697.471 kb on - strand, within MMJJ_RS03735 at 697.471 kb on - strand, within MMJJ_RS03735
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 694,816 - MMJJ_RS03715 0.74 +0.7 694,817 + -0.7 694,839 - MMJJ_RS03715 0.78 +2.7 694,850 - MMJJ_RS03715 0.80 -2.3 694,850 - MMJJ_RS03715 0.80 +0.4 694,850 - MMJJ_RS03715 0.80 -1.2 694,851 + MMJJ_RS03715 0.80 -1.6 694,851 + +0.9 694,851 + MMJJ_RS03715 0.80 +1.7 694,860 - MMJJ_RS03715 0.81 +2.4 694,870 - MMJJ_RS03715 0.83 -0.8 694,875 - MMJJ_RS03715 0.84 +1.7 694,875 - MMJJ_RS03715 0.84 +2.1 694,876 + MMJJ_RS03715 0.84 -2.6 694,886 - MMJJ_RS03715 0.86 -0.5 694,887 + MMJJ_RS03715 0.86 +1.3 694,890 - MMJJ_RS03715 0.86 -2.7 694,891 + MMJJ_RS03715 0.87 +3.7 694,940 + +0.1 694,963 + +1.9 694,968 - -0.9 694,969 + +2.0 694,969 + -0.5 694,984 - +0.4 694,985 + +0.5 694,990 + +0.8 694,994 - -2.5 695,013 - +3.6 695,027 - +0.6 695,033 - -0.1 695,033 - +1.0 695,033 - +3.7 695,059 - -0.0 695,060 + -3.3 695,101 + -2.3 695,122 - -2.8 695,123 + +1.9 695,131 - MMJJ_RS03720 0.11 +0.5 695,132 + MMJJ_RS03720 0.12 +0.9 695,134 - MMJJ_RS03720 0.12 +1.8 695,135 + MMJJ_RS03720 0.12 +3.7 695,221 - MMJJ_RS03720 0.37 +2.4 695,222 + MMJJ_RS03720 0.38 -1.8 695,264 - MMJJ_RS03720 0.50 +0.5 695,265 + MMJJ_RS03720 0.50 -1.9 695,306 - MMJJ_RS03720 0.62 +0.5 695,307 + MMJJ_RS03720 0.62 +2.2 695,307 + MMJJ_RS03720 0.62 +3.0 695,329 - MMJJ_RS03720 0.69 +1.5 695,330 + MMJJ_RS03720 0.69 -0.1 695,330 + MMJJ_RS03720 0.69 +2.9 695,331 - MMJJ_RS03720 0.69 -0.9 695,362 - MMJJ_RS03720 0.78 +3.6 695,362 - MMJJ_RS03720 0.78 +0.6 695,374 - MMJJ_RS03720 0.82 -0.2 695,416 - +2.1 695,419 - -1.0 695,419 - +0.6 695,420 + +1.4 695,420 + +0.9 695,421 - +2.1 695,457 - +0.5 695,458 + +0.7 695,458 + +0.9 695,462 + +3.6 695,462 + +2.1 695,498 + MMJJ_RS03725 0.24 +2.4 695,511 - MMJJ_RS03725 0.31 +2.9 695,511 - MMJJ_RS03725 0.31 +3.2 695,632 - MMJJ_RS03725 0.87 +0.4 695,681 - -2.5 695,687 - +1.3 695,732 + -0.6 695,747 + +2.0 695,755 + +0.8 695,763 - +0.1 695,763 - +0.4 695,763 - -1.9 695,763 - -0.5 695,763 - +0.6 695,764 + +1.6 695,764 + +0.2 695,764 + -0.9 695,764 + +0.2 695,768 + -1.0 695,771 - +0.7 695,784 - +1.0 695,837 - -3.9 695,838 + +1.7 695,838 + -2.5 695,844 + -0.7 695,863 - -1.9 695,864 + -0.4 695,864 + +0.5 695,883 + +1.2 695,917 + MMJJ_RS03730 0.15 +1.9 695,956 - MMJJ_RS03730 0.20 -1.6 695,968 - MMJJ_RS03730 0.22 +2.5 695,999 - MMJJ_RS03730 0.27 -1.8 696,000 + MMJJ_RS03730 0.27 -1.1 696,000 + MMJJ_RS03730 0.27 +1.1 696,000 + MMJJ_RS03730 0.27 -2.0 696,085 + MMJJ_RS03730 0.39 +0.7 696,229 - MMJJ_RS03730 0.60 -0.5 696,321 + MMJJ_RS03730 0.73 +0.1 696,488 - -1.7 696,488 - +1.9 696,488 - +0.0 696,488 - +2.6 696,488 - -1.3 696,488 - -0.1 696,488 - +0.6 696,488 - +0.4 696,488 - -0.1 696,488 - +0.6 696,489 + -0.8 696,489 + +1.7 696,489 + +1.6 696,489 + +1.6 696,489 + +0.0 696,489 + +1.0 696,489 + -0.8 696,490 - +2.7 696,490 - -0.2 696,490 - -0.8 696,490 - -2.1 696,490 - +2.2 696,490 - +2.5 696,490 - -1.0 696,490 - -0.9 696,490 - -3.0 696,490 - +1.3 696,490 - -0.1 696,490 - -1.8 696,490 - -1.3 696,490 - +0.8 696,491 + -4.2 696,491 + -1.0 696,491 + -0.7 696,491 + -1.7 696,491 + +2.4 696,491 + +3.6 696,491 + -2.3 696,491 + +0.7 696,491 + +1.1 696,491 + +0.4 696,491 + +1.4 696,528 - -1.4 696,528 - +0.9 696,528 - +1.6 696,530 - -1.1 696,532 - +0.1 696,532 - +1.2 696,532 - -1.4 696,533 + +1.6 696,533 + +0.2 696,533 + +1.8 696,533 + -2.3 696,534 - -0.5 696,534 - +0.1 696,535 + -2.9 696,550 - -1.1 696,550 - -2.0 696,550 - +1.4 696,550 - +2.8 696,551 + -0.5 696,551 + -0.2 696,551 + +2.6 696,552 - +1.4 696,553 + +0.9 696,553 + +0.4 696,558 - +1.5 696,558 - -0.7 696,558 - +0.3 696,576 - -1.1 696,577 + +0.2 696,632 - -0.7 696,663 + -2.4 696,679 - -1.7 696,679 - -0.4 696,680 + +0.4 696,680 + -0.6 696,686 - +1.8 696,686 - -1.4 696,686 - +0.1 696,687 + +3.9 696,694 + +1.9 696,703 - -0.4 696,704 + +1.4 696,704 + +0.4 696,704 + +0.9 696,746 - -2.0 696,746 - -1.7 696,849 - MMJJ_RS03735 0.16 +1.0 696,876 - MMJJ_RS03735 0.17 -0.1 696,932 - MMJJ_RS03735 0.21 +1.0 696,933 + MMJJ_RS03735 0.21 +0.6 696,933 + MMJJ_RS03735 0.21 +1.5 696,987 + MMJJ_RS03735 0.24 +2.0 697,080 + MMJJ_RS03735 0.29 +0.3 697,100 + -1.1 697,118 - MMJJ_RS03735 0.32 +0.4 697,137 + MMJJ_RS03735 0.33 +4.1 697,137 + MMJJ_RS03735 0.33 -3.1 697,184 - MMJJ_RS03735 0.35 -3.0 697,191 - MMJJ_RS03735 0.36 +1.4 697,230 - MMJJ_RS03735 0.38 +2.4 697,230 - MMJJ_RS03735 0.38 -1.0 697,231 + MMJJ_RS03735 0.38 -0.9 697,311 - MMJJ_RS03735 0.43 -0.4 697,311 - MMJJ_RS03735 0.43 +0.3 697,316 - MMJJ_RS03735 0.43 +3.4 697,317 + MMJJ_RS03735 0.43 +2.5 697,350 - MMJJ_RS03735 0.45 -1.7 697,350 - MMJJ_RS03735 0.45 +0.7 697,392 - MMJJ_RS03735 0.48 +0.9 697,392 - MMJJ_RS03735 0.48 +0.9 697,396 + MMJJ_RS03735 0.48 -1.3 697,470 + MMJJ_RS03735 0.52 -1.4 697,471 - MMJJ_RS03735 0.52 +1.6 697,471 - MMJJ_RS03735 0.52 +0.6
Or see this region's nucleotide sequence