Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03215 and MMJJ_RS03220 are separated by 65 nucleotides MMJJ_RS03220 and MMJJ_RS03225 are separated by 86 nucleotides MMJJ_RS03225 and MMJJ_RS03230 are separated by 2 nucleotides
MMJJ_RS03215: MMJJ_RS03215 - alanine--glyoxylate aminotransferase family protein, at 609,411 to 610,559
_RS03215
MMJJ_RS03220: MMJJ_RS03220 - inosine/xanthosine triphosphatase, at 610,625 to 611,284
_RS03220
MMJJ_RS03225: MMJJ_RS03225 - 4Fe-4S binding protein, at 611,371 to 611,535
_RS03225
MMJJ_RS03230: MMJJ_RS03230 - NAD(P)/FAD-dependent oxidoreductase, at 611,538 to 612,710
_RS03230
Position (kb)
610
611
612 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 609.911 kb on + strand, within MMJJ_RS03215 at 609.944 kb on + strand at 610.159 kb on + strand at 610.550 kb on + strand at 610.557 kb on - strand at 610.565 kb on + strand at 610.581 kb on - strand at 610.600 kb on + strand at 610.604 kb on - strand at 610.605 kb on + strand at 610.605 kb on + strand at 610.623 kb on - strand at 610.624 kb on + strand at 610.690 kb on + strand at 610.690 kb on + strand at 610.766 kb on + strand, within MMJJ_RS03220 at 610.845 kb on - strand, within MMJJ_RS03220 at 610.845 kb on - strand, within MMJJ_RS03220 at 610.846 kb on + strand, within MMJJ_RS03220 at 610.846 kb on + strand, within MMJJ_RS03220 at 610.907 kb on + strand, within MMJJ_RS03220 at 610.916 kb on - strand, within MMJJ_RS03220 at 610.916 kb on - strand, within MMJJ_RS03220 at 610.916 kb on - strand, within MMJJ_RS03220 at 610.916 kb on - strand, within MMJJ_RS03220 at 610.917 kb on + strand, within MMJJ_RS03220 at 610.917 kb on + strand, within MMJJ_RS03220 at 610.917 kb on + strand, within MMJJ_RS03220 at 610.918 kb on - strand, within MMJJ_RS03220 at 610.948 kb on + strand, within MMJJ_RS03220 at 611.028 kb on - strand, within MMJJ_RS03220 at 611.028 kb on - strand, within MMJJ_RS03220 at 611.029 kb on + strand, within MMJJ_RS03220 at 611.029 kb on + strand, within MMJJ_RS03220 at 611.029 kb on + strand, within MMJJ_RS03220 at 611.029 kb on + strand, within MMJJ_RS03220 at 611.030 kb on - strand, within MMJJ_RS03220 at 611.030 kb on - strand, within MMJJ_RS03220 at 611.031 kb on + strand, within MMJJ_RS03220 at 611.032 kb on - strand, within MMJJ_RS03220 at 611.032 kb on - strand, within MMJJ_RS03220 at 611.032 kb on - strand, within MMJJ_RS03220 at 611.033 kb on + strand, within MMJJ_RS03220 at 611.033 kb on + strand, within MMJJ_RS03220 at 611.086 kb on - strand, within MMJJ_RS03220 at 611.188 kb on - strand, within MMJJ_RS03220 at 611.200 kb on - strand, within MMJJ_RS03220 at 611.200 kb on - strand, within MMJJ_RS03220 at 611.200 kb on - strand, within MMJJ_RS03220 at 611.200 kb on - strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.203 kb on + strand, within MMJJ_RS03220 at 611.203 kb on + strand, within MMJJ_RS03220 at 611.203 kb on + strand, within MMJJ_RS03220 at 611.207 kb on + strand, within MMJJ_RS03220 at 611.217 kb on - strand, within MMJJ_RS03220 at 611.217 kb on - strand, within MMJJ_RS03220 at 611.217 kb on - strand, within MMJJ_RS03220 at 611.217 kb on - strand, within MMJJ_RS03220 at 611.218 kb on + strand, within MMJJ_RS03220 at 611.218 kb on + strand, within MMJJ_RS03220 at 611.218 kb on + strand, within MMJJ_RS03220 at 611.218 kb on + strand, within MMJJ_RS03220 at 611.221 kb on - strand at 611.235 kb on - strand at 611.235 kb on - strand at 611.236 kb on + strand at 611.255 kb on - strand at 611.256 kb on + strand at 611.275 kb on + strand at 611.282 kb on - strand at 611.292 kb on - strand at 611.295 kb on - strand at 611.295 kb on - strand at 611.296 kb on + strand at 611.296 kb on + strand at 611.317 kb on - strand at 611.318 kb on + strand at 611.318 kb on + strand at 611.321 kb on - strand at 611.321 kb on - strand at 611.321 kb on - strand at 611.324 kb on + strand at 611.324 kb on + strand at 611.324 kb on + strand at 611.342 kb on + strand at 611.420 kb on - strand, within MMJJ_RS03225 at 611.425 kb on + strand, within MMJJ_RS03225 at 611.499 kb on - strand, within MMJJ_RS03225 at 611.507 kb on - strand, within MMJJ_RS03225 at 611.507 kb on - strand, within MMJJ_RS03225 at 611.507 kb on - strand, within MMJJ_RS03225 at 611.560 kb on + strand at 611.641 kb on - strand at 612.012 kb on + strand, within MMJJ_RS03230 at 612.153 kb on - strand, within MMJJ_RS03230 at 612.180 kb on + strand, within MMJJ_RS03230 at 612.191 kb on + strand, within MMJJ_RS03230 at 612.235 kb on - strand, within MMJJ_RS03230 at 612.235 kb on - strand, within MMJJ_RS03230 at 612.236 kb on + strand, within MMJJ_RS03230
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 609,911 + MMJJ_RS03215 0.44 -0.8 609,944 + +0.8 610,159 + -0.8 610,550 + -3.3 610,557 - -0.8 610,565 + +1.1 610,581 - +1.3 610,600 + -2.3 610,604 - -0.2 610,605 + +0.7 610,605 + -0.2 610,623 - -1.9 610,624 + -0.8 610,690 + +0.9 610,690 + -0.1 610,766 + MMJJ_RS03220 0.21 +1.1 610,845 - MMJJ_RS03220 0.33 -1.4 610,845 - MMJJ_RS03220 0.33 +0.3 610,846 + MMJJ_RS03220 0.33 +2.6 610,846 + MMJJ_RS03220 0.33 -2.5 610,907 + MMJJ_RS03220 0.43 +1.6 610,916 - MMJJ_RS03220 0.44 -1.1 610,916 - MMJJ_RS03220 0.44 -0.0 610,916 - MMJJ_RS03220 0.44 +0.5 610,916 - MMJJ_RS03220 0.44 +1.8 610,917 + MMJJ_RS03220 0.44 +2.3 610,917 + MMJJ_RS03220 0.44 -2.1 610,917 + MMJJ_RS03220 0.44 +1.4 610,918 - MMJJ_RS03220 0.44 +1.2 610,948 + MMJJ_RS03220 0.49 +1.4 611,028 - MMJJ_RS03220 0.61 -1.3 611,028 - MMJJ_RS03220 0.61 +0.4 611,029 + MMJJ_RS03220 0.61 -3.8 611,029 + MMJJ_RS03220 0.61 -2.0 611,029 + MMJJ_RS03220 0.61 +0.4 611,029 + MMJJ_RS03220 0.61 +0.8 611,030 - MMJJ_RS03220 0.61 -0.4 611,030 - MMJJ_RS03220 0.61 -0.5 611,031 + MMJJ_RS03220 0.62 -1.1 611,032 - MMJJ_RS03220 0.62 -0.0 611,032 - MMJJ_RS03220 0.62 +1.4 611,032 - MMJJ_RS03220 0.62 +1.2 611,033 + MMJJ_RS03220 0.62 +0.9 611,033 + MMJJ_RS03220 0.62 +0.2 611,086 - MMJJ_RS03220 0.70 -2.0 611,188 - MMJJ_RS03220 0.85 -1.9 611,200 - MMJJ_RS03220 0.87 -0.8 611,200 - MMJJ_RS03220 0.87 +1.2 611,200 - MMJJ_RS03220 0.87 -0.1 611,200 - MMJJ_RS03220 0.87 -0.5 611,201 + MMJJ_RS03220 0.87 +0.3 611,201 + MMJJ_RS03220 0.87 -1.2 611,201 + MMJJ_RS03220 0.87 -3.6 611,201 + MMJJ_RS03220 0.87 +2.1 611,201 + MMJJ_RS03220 0.87 -0.3 611,202 - MMJJ_RS03220 0.87 -0.5 611,202 - MMJJ_RS03220 0.87 -0.1 611,202 - MMJJ_RS03220 0.87 +1.6 611,202 - MMJJ_RS03220 0.87 +0.2 611,202 - MMJJ_RS03220 0.87 -0.5 611,203 + MMJJ_RS03220 0.88 -4.0 611,203 + MMJJ_RS03220 0.88 -3.1 611,203 + MMJJ_RS03220 0.88 -0.6 611,207 + MMJJ_RS03220 0.88 -2.6 611,217 - MMJJ_RS03220 0.90 -2.3 611,217 - MMJJ_RS03220 0.90 +3.3 611,217 - MMJJ_RS03220 0.90 -2.8 611,217 - MMJJ_RS03220 0.90 -1.9 611,218 + MMJJ_RS03220 0.90 +2.0 611,218 + MMJJ_RS03220 0.90 -1.4 611,218 + MMJJ_RS03220 0.90 +0.2 611,218 + MMJJ_RS03220 0.90 +1.9 611,221 - +0.1 611,235 - -1.1 611,235 - -1.8 611,236 + +0.1 611,255 - -1.0 611,256 + +1.2 611,275 + +2.0 611,282 - +0.4 611,292 - -3.9 611,295 - -3.0 611,295 - -2.5 611,296 + -1.2 611,296 + -3.4 611,317 - -2.7 611,318 + -1.2 611,318 + -0.2 611,321 - -2.2 611,321 - -1.5 611,321 - -0.8 611,324 + -2.6 611,324 + -1.8 611,324 + -0.8 611,342 + -2.0 611,420 - MMJJ_RS03225 0.30 -0.2 611,425 + MMJJ_RS03225 0.33 -1.5 611,499 - MMJJ_RS03225 0.78 -3.5 611,507 - MMJJ_RS03225 0.82 -0.8 611,507 - MMJJ_RS03225 0.82 -2.3 611,507 - MMJJ_RS03225 0.82 -0.8 611,560 + -0.8 611,641 - -0.2 612,012 + MMJJ_RS03230 0.40 -0.8 612,153 - MMJJ_RS03230 0.52 -1.2 612,180 + MMJJ_RS03230 0.55 -1.8 612,191 + MMJJ_RS03230 0.56 +0.8 612,235 - MMJJ_RS03230 0.59 -1.8 612,235 - MMJJ_RS03230 0.59 -2.8 612,236 + MMJJ_RS03230 0.60 -1.8
Or see this region's nucleotide sequence