Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS03215

Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS03210 and MMJJ_RS03215 are separated by 98 nucleotidesMMJJ_RS03215 and MMJJ_RS03220 are separated by 65 nucleotidesMMJJ_RS03220 and MMJJ_RS03225 are separated by 86 nucleotidesMMJJ_RS03225 and MMJJ_RS03230 are separated by 2 nucleotides MMJJ_RS03210: MMJJ_RS03210 - phosphoribosylamine--glycine ligase, at 607,978 to 609,312 _RS03210 MMJJ_RS03215: MMJJ_RS03215 - alanine--glyoxylate aminotransferase family protein, at 609,411 to 610,559 _RS03215 MMJJ_RS03220: MMJJ_RS03220 - inosine/xanthosine triphosphatase, at 610,625 to 611,284 _RS03220 MMJJ_RS03225: MMJJ_RS03225 - 4Fe-4S binding protein, at 611,371 to 611,535 _RS03225 MMJJ_RS03230: MMJJ_RS03230 - NAD(P)/FAD-dependent oxidoreductase, at 611,538 to 612,710 _RS03230 Position (kb) 609 610 611Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 608.786 kb on - strandat 609.004 kb on - strandat 609.004 kb on - strand, within MMJJ_RS03210at 609.273 kb on - strandat 609.285 kb on + strandat 609.310 kb on - strandat 609.311 kb on + strandat 609.311 kb on + strandat 609.311 kb on + strandat 609.314 kb on - strandat 609.315 kb on + strandat 609.317 kb on + strandat 609.331 kb on - strandat 609.911 kb on + strand, within MMJJ_RS03215at 609.944 kb on + strandat 610.159 kb on + strandat 610.550 kb on + strandat 610.557 kb on - strandat 610.565 kb on + strandat 610.581 kb on - strandat 610.600 kb on + strandat 610.604 kb on - strandat 610.605 kb on + strandat 610.605 kb on + strandat 610.623 kb on - strandat 610.624 kb on + strandat 610.690 kb on + strandat 610.690 kb on + strandat 610.766 kb on + strand, within MMJJ_RS03220at 610.845 kb on - strand, within MMJJ_RS03220at 610.845 kb on - strand, within MMJJ_RS03220at 610.846 kb on + strand, within MMJJ_RS03220at 610.846 kb on + strand, within MMJJ_RS03220at 610.907 kb on + strand, within MMJJ_RS03220at 610.916 kb on - strand, within MMJJ_RS03220at 610.916 kb on - strand, within MMJJ_RS03220at 610.916 kb on - strand, within MMJJ_RS03220at 610.916 kb on - strand, within MMJJ_RS03220at 610.917 kb on + strand, within MMJJ_RS03220at 610.917 kb on + strand, within MMJJ_RS03220at 610.917 kb on + strand, within MMJJ_RS03220at 610.918 kb on - strand, within MMJJ_RS03220at 610.948 kb on + strand, within MMJJ_RS03220at 611.028 kb on - strand, within MMJJ_RS03220at 611.028 kb on - strand, within MMJJ_RS03220at 611.029 kb on + strand, within MMJJ_RS03220at 611.029 kb on + strand, within MMJJ_RS03220at 611.029 kb on + strand, within MMJJ_RS03220at 611.029 kb on + strand, within MMJJ_RS03220at 611.030 kb on - strand, within MMJJ_RS03220at 611.030 kb on - strand, within MMJJ_RS03220at 611.031 kb on + strand, within MMJJ_RS03220at 611.032 kb on - strand, within MMJJ_RS03220at 611.032 kb on - strand, within MMJJ_RS03220at 611.032 kb on - strand, within MMJJ_RS03220at 611.033 kb on + strand, within MMJJ_RS03220at 611.033 kb on + strand, within MMJJ_RS03220at 611.086 kb on - strand, within MMJJ_RS03220at 611.188 kb on - strand, within MMJJ_RS03220at 611.200 kb on - strand, within MMJJ_RS03220at 611.200 kb on - strand, within MMJJ_RS03220at 611.200 kb on - strand, within MMJJ_RS03220at 611.200 kb on - strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.201 kb on + strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.202 kb on - strand, within MMJJ_RS03220at 611.203 kb on + strand, within MMJJ_RS03220at 611.203 kb on + strand, within MMJJ_RS03220at 611.203 kb on + strand, within MMJJ_RS03220at 611.207 kb on + strand, within MMJJ_RS03220at 611.217 kb on - strand, within MMJJ_RS03220at 611.217 kb on - strand, within MMJJ_RS03220at 611.217 kb on - strand, within MMJJ_RS03220at 611.217 kb on - strand, within MMJJ_RS03220at 611.218 kb on + strand, within MMJJ_RS03220at 611.218 kb on + strand, within MMJJ_RS03220at 611.218 kb on + strand, within MMJJ_RS03220at 611.218 kb on + strand, within MMJJ_RS03220at 611.221 kb on - strandat 611.235 kb on - strandat 611.235 kb on - strandat 611.236 kb on + strandat 611.255 kb on - strandat 611.256 kb on + strandat 611.275 kb on + strandat 611.282 kb on - strandat 611.292 kb on - strandat 611.295 kb on - strandat 611.295 kb on - strandat 611.296 kb on + strandat 611.296 kb on + strandat 611.317 kb on - strandat 611.318 kb on + strandat 611.318 kb on + strandat 611.321 kb on - strandat 611.321 kb on - strandat 611.321 kb on - strandat 611.324 kb on + strandat 611.324 kb on + strandat 611.324 kb on + strandat 611.342 kb on + strandat 611.420 kb on - strand, within MMJJ_RS03225at 611.425 kb on + strand, within MMJJ_RS03225at 611.499 kb on - strand, within MMJJ_RS03225at 611.507 kb on - strand, within MMJJ_RS03225at 611.507 kb on - strand, within MMJJ_RS03225at 611.507 kb on - strand, within MMJJ_RS03225

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM
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608,786 - +0.8
609,004 - +0.8
609,004 - MMJJ_RS03210 0.77 +0.2
609,273 - -1.2
609,285 + -1.4
609,310 - +2.0
609,311 + -1.3
609,311 + +1.5
609,311 + -0.1
609,314 - +0.1
609,315 + +0.7
609,317 + -1.2
609,331 - +0.8
609,911 + MMJJ_RS03215 0.44 -0.8
609,944 + +0.8
610,159 + -0.8
610,550 + -3.3
610,557 - -0.8
610,565 + +1.1
610,581 - +1.3
610,600 + -2.3
610,604 - -0.2
610,605 + +0.7
610,605 + -0.2
610,623 - -1.9
610,624 + -0.8
610,690 + +0.9
610,690 + -0.1
610,766 + MMJJ_RS03220 0.21 +1.1
610,845 - MMJJ_RS03220 0.33 -1.4
610,845 - MMJJ_RS03220 0.33 +0.3
610,846 + MMJJ_RS03220 0.33 +2.6
610,846 + MMJJ_RS03220 0.33 -2.5
610,907 + MMJJ_RS03220 0.43 +1.6
610,916 - MMJJ_RS03220 0.44 -1.1
610,916 - MMJJ_RS03220 0.44 -0.0
610,916 - MMJJ_RS03220 0.44 +0.5
610,916 - MMJJ_RS03220 0.44 +1.8
610,917 + MMJJ_RS03220 0.44 +2.3
610,917 + MMJJ_RS03220 0.44 -2.1
610,917 + MMJJ_RS03220 0.44 +1.4
610,918 - MMJJ_RS03220 0.44 +1.2
610,948 + MMJJ_RS03220 0.49 +1.4
611,028 - MMJJ_RS03220 0.61 -1.3
611,028 - MMJJ_RS03220 0.61 +0.4
611,029 + MMJJ_RS03220 0.61 -3.8
611,029 + MMJJ_RS03220 0.61 -2.0
611,029 + MMJJ_RS03220 0.61 +0.4
611,029 + MMJJ_RS03220 0.61 +0.8
611,030 - MMJJ_RS03220 0.61 -0.4
611,030 - MMJJ_RS03220 0.61 -0.5
611,031 + MMJJ_RS03220 0.62 -1.1
611,032 - MMJJ_RS03220 0.62 -0.0
611,032 - MMJJ_RS03220 0.62 +1.4
611,032 - MMJJ_RS03220 0.62 +1.2
611,033 + MMJJ_RS03220 0.62 +0.9
611,033 + MMJJ_RS03220 0.62 +0.2
611,086 - MMJJ_RS03220 0.70 -2.0
611,188 - MMJJ_RS03220 0.85 -1.9
611,200 - MMJJ_RS03220 0.87 -0.8
611,200 - MMJJ_RS03220 0.87 +1.2
611,200 - MMJJ_RS03220 0.87 -0.1
611,200 - MMJJ_RS03220 0.87 -0.5
611,201 + MMJJ_RS03220 0.87 +0.3
611,201 + MMJJ_RS03220 0.87 -1.2
611,201 + MMJJ_RS03220 0.87 -3.6
611,201 + MMJJ_RS03220 0.87 +2.1
611,201 + MMJJ_RS03220 0.87 -0.3
611,202 - MMJJ_RS03220 0.87 -0.5
611,202 - MMJJ_RS03220 0.87 -0.1
611,202 - MMJJ_RS03220 0.87 +1.6
611,202 - MMJJ_RS03220 0.87 +0.2
611,202 - MMJJ_RS03220 0.87 -0.5
611,203 + MMJJ_RS03220 0.88 -4.0
611,203 + MMJJ_RS03220 0.88 -3.1
611,203 + MMJJ_RS03220 0.88 -0.6
611,207 + MMJJ_RS03220 0.88 -2.6
611,217 - MMJJ_RS03220 0.90 -2.3
611,217 - MMJJ_RS03220 0.90 +3.3
611,217 - MMJJ_RS03220 0.90 -2.8
611,217 - MMJJ_RS03220 0.90 -1.9
611,218 + MMJJ_RS03220 0.90 +2.0
611,218 + MMJJ_RS03220 0.90 -1.4
611,218 + MMJJ_RS03220 0.90 +0.2
611,218 + MMJJ_RS03220 0.90 +1.9
611,221 - +0.1
611,235 - -1.1
611,235 - -1.8
611,236 + +0.1
611,255 - -1.0
611,256 + +1.2
611,275 + +2.0
611,282 - +0.4
611,292 - -3.9
611,295 - -3.0
611,295 - -2.5
611,296 + -1.2
611,296 + -3.4
611,317 - -2.7
611,318 + -1.2
611,318 + -0.2
611,321 - -2.2
611,321 - -1.5
611,321 - -0.8
611,324 + -2.6
611,324 + -1.8
611,324 + -0.8
611,342 + -2.0
611,420 - MMJJ_RS03225 0.30 -0.2
611,425 + MMJJ_RS03225 0.33 -1.5
611,499 - MMJJ_RS03225 0.78 -3.5
611,507 - MMJJ_RS03225 0.82 -0.8
611,507 - MMJJ_RS03225 0.82 -2.3
611,507 - MMJJ_RS03225 0.82 -0.8

Or see this region's nucleotide sequence