Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03200 and MMJJ_RS03205 are separated by 58 nucleotides MMJJ_RS03205 and MMJJ_RS03210 are separated by 200 nucleotides MMJJ_RS03210 and MMJJ_RS03215 are separated by 98 nucleotides
MMJJ_RS03200: MMJJ_RS03200 - uroporphyrinogen-III synthase, at 606,469 to 607,203
_RS03200
MMJJ_RS03205: MMJJ_RS03205 - metallophosphoesterase, at 607,262 to 607,777
_RS03205
MMJJ_RS03210: MMJJ_RS03210 - phosphoribosylamine--glycine ligase, at 607,978 to 609,312
_RS03210
MMJJ_RS03215: MMJJ_RS03215 - alanine--glyoxylate aminotransferase family protein, at 609,411 to 610,559
_RS03215
Position (kb) 607
608
609
610 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 607.078 kb on - strand, within MMJJ_RS03200 at 607.080 kb on - strand, within MMJJ_RS03200 at 607.182 kb on + strand at 607.193 kb on - strand at 607.214 kb on - strand at 607.214 kb on - strand at 607.215 kb on + strand at 607.215 kb on + strand at 607.215 kb on + strand at 607.217 kb on + strand at 607.220 kb on - strand at 607.225 kb on - strand at 607.225 kb on - strand at 607.232 kb on - strand at 607.232 kb on - strand at 607.232 kb on - strand at 607.232 kb on - strand at 607.232 kb on - strand at 607.233 kb on + strand at 607.236 kb on - strand at 607.236 kb on - strand at 607.236 kb on - strand at 607.236 kb on - strand at 607.237 kb on + strand at 607.237 kb on + strand at 607.245 kb on - strand at 607.248 kb on - strand at 607.262 kb on + strand at 607.273 kb on - strand at 607.295 kb on - strand at 607.295 kb on - strand at 607.296 kb on + strand at 607.296 kb on + strand at 607.299 kb on - strand at 607.300 kb on + strand at 607.307 kb on + strand at 607.308 kb on - strand at 607.309 kb on + strand at 607.348 kb on - strand, within MMJJ_RS03205 at 607.408 kb on + strand, within MMJJ_RS03205 at 607.474 kb on + strand, within MMJJ_RS03205 at 607.509 kb on + strand, within MMJJ_RS03205 at 607.512 kb on - strand, within MMJJ_RS03205 at 607.513 kb on + strand, within MMJJ_RS03205 at 607.534 kb on - strand, within MMJJ_RS03205 at 607.534 kb on - strand, within MMJJ_RS03205 at 607.535 kb on + strand, within MMJJ_RS03205 at 607.581 kb on - strand, within MMJJ_RS03205 at 607.582 kb on + strand, within MMJJ_RS03205 at 607.585 kb on + strand, within MMJJ_RS03205 at 607.608 kb on - strand, within MMJJ_RS03205 at 607.608 kb on - strand, within MMJJ_RS03205 at 607.659 kb on - strand, within MMJJ_RS03205 at 607.659 kb on - strand, within MMJJ_RS03205 at 607.666 kb on - strand, within MMJJ_RS03205 at 607.719 kb on - strand, within MMJJ_RS03205 at 607.719 kb on - strand, within MMJJ_RS03205 at 607.720 kb on + strand, within MMJJ_RS03205 at 607.739 kb on - strand at 607.756 kb on + strand at 607.785 kb on - strand at 607.785 kb on - strand at 607.785 kb on - strand at 607.786 kb on + strand at 607.812 kb on + strand at 607.812 kb on + strand at 607.817 kb on - strand at 607.818 kb on + strand at 607.821 kb on - strand at 607.822 kb on + strand at 607.826 kb on - strand at 607.826 kb on - strand at 607.826 kb on - strand at 607.827 kb on + strand at 607.839 kb on + strand at 607.839 kb on + strand at 607.848 kb on - strand at 607.854 kb on - strand at 607.855 kb on + strand at 607.855 kb on + strand at 607.861 kb on - strand at 607.862 kb on + strand at 607.862 kb on + strand at 607.863 kb on - strand at 607.863 kb on - strand at 607.863 kb on - strand at 607.887 kb on + strand at 607.892 kb on + strand at 607.892 kb on + strand at 608.091 kb on + strand at 608.093 kb on + strand at 608.786 kb on - strand at 609.004 kb on - strand at 609.004 kb on - strand, within MMJJ_RS03210 at 609.273 kb on - strand at 609.285 kb on + strand at 609.310 kb on - strand at 609.311 kb on + strand at 609.311 kb on + strand at 609.311 kb on + strand at 609.314 kb on - strand at 609.315 kb on + strand at 609.317 kb on + strand at 609.331 kb on - strand at 609.911 kb on + strand, within MMJJ_RS03215 at 609.944 kb on + strand at 610.159 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 607,078 - MMJJ_RS03200 0.83 -0.8 607,080 - MMJJ_RS03200 0.83 -2.2 607,182 + +2.2 607,193 - +0.8 607,214 - -2.7 607,214 - -2.1 607,215 + -3.0 607,215 + -1.8 607,215 + -2.8 607,217 + -0.9 607,220 - -3.9 607,225 - -0.3 607,225 - -1.5 607,232 - -2.3 607,232 - -3.3 607,232 - -1.3 607,232 - -2.8 607,232 - -4.7 607,233 + -2.1 607,236 - -2.4 607,236 - -0.5 607,236 - -1.9 607,236 - -0.7 607,237 + -2.6 607,237 + -1.2 607,245 - -1.7 607,248 - -1.0 607,262 + -2.7 607,273 - -1.5 607,295 - -2.6 607,295 - -1.6 607,296 + -3.7 607,296 + -3.0 607,299 - -3.1 607,300 + -5.2 607,307 + -3.2 607,308 - -4.8 607,309 + -0.7 607,348 - MMJJ_RS03205 0.17 -2.8 607,408 + MMJJ_RS03205 0.28 -1.7 607,474 + MMJJ_RS03205 0.41 -3.0 607,509 + MMJJ_RS03205 0.48 -1.7 607,512 - MMJJ_RS03205 0.48 -3.3 607,513 + MMJJ_RS03205 0.49 -2.6 607,534 - MMJJ_RS03205 0.53 -0.4 607,534 - MMJJ_RS03205 0.53 -4.3 607,535 + MMJJ_RS03205 0.53 -4.2 607,581 - MMJJ_RS03205 0.62 -3.3 607,582 + MMJJ_RS03205 0.62 -2.1 607,585 + MMJJ_RS03205 0.63 -2.5 607,608 - MMJJ_RS03205 0.67 -1.8 607,608 - MMJJ_RS03205 0.67 -3.6 607,659 - MMJJ_RS03205 0.77 -2.3 607,659 - MMJJ_RS03205 0.77 -3.4 607,666 - MMJJ_RS03205 0.78 -3.6 607,719 - MMJJ_RS03205 0.89 -3.3 607,719 - MMJJ_RS03205 0.89 -0.7 607,720 + MMJJ_RS03205 0.89 -0.2 607,739 - -3.2 607,756 + -0.3 607,785 - -2.8 607,785 - -2.0 607,785 - +2.2 607,786 + -1.4 607,812 + -0.6 607,812 + +0.3 607,817 - +2.9 607,818 + +1.8 607,821 - -0.4 607,822 + +1.2 607,826 - -0.3 607,826 - -3.4 607,826 - -2.3 607,827 + -3.8 607,839 + -0.7 607,839 + +0.7 607,848 - +0.1 607,854 - +1.0 607,855 + +1.3 607,855 + -1.2 607,861 - +1.1 607,862 + -1.8 607,862 + -0.8 607,863 - -2.2 607,863 - +1.0 607,863 - +0.8 607,887 + +0.6 607,892 + +3.6 607,892 + +2.1 608,091 + -1.2 608,093 + -0.2 608,786 - +0.8 609,004 - +0.8 609,004 - MMJJ_RS03210 0.77 +0.2 609,273 - -1.2 609,285 + -1.4 609,310 - +2.0 609,311 + -1.3 609,311 + +1.5 609,311 + -0.1 609,314 - +0.1 609,315 + +0.7 609,317 + -1.2 609,331 - +0.8 609,911 + MMJJ_RS03215 0.44 -0.8 609,944 + +0.8 610,159 + -0.8
Or see this region's nucleotide sequence