Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS02175

Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS02165 and MMJJ_RS02170 are separated by 169 nucleotidesMMJJ_RS02170 and MMJJ_RS02175 are separated by 9 nucleotidesMMJJ_RS02175 and MMJJ_RS02180 are separated by 75 nucleotides MMJJ_RS02165: MMJJ_RS02165 - ABC transporter substrate-binding protein, at 411,074 to 411,868 _RS02165 MMJJ_RS02170: MMJJ_RS02170 - 2-thiouracil desulfurase family protein, at 412,038 to 412,586 _RS02170 MMJJ_RS02175: MMJJ_RS02175 - imidazoleglycerol-phosphate dehydratase HisB, at 412,596 to 413,168 _RS02175 MMJJ_RS02180: MMJJ_RS02180 - DHH family phosphoesterase, at 413,244 to 414,539 _RS02180 Position (kb) 412 413 414Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 411.656 kb on - strand, within MMJJ_RS02165at 411.657 kb on + strand, within MMJJ_RS02165at 411.657 kb on + strand, within MMJJ_RS02165at 411.735 kb on + strand, within MMJJ_RS02165at 411.854 kb on - strandat 411.860 kb on + strandat 411.916 kb on - strandat 411.991 kb on + strandat 412.039 kb on - strandat 412.110 kb on - strand, within MMJJ_RS02170at 412.149 kb on + strand, within MMJJ_RS02170at 412.211 kb on - strand, within MMJJ_RS02170at 412.211 kb on - strand, within MMJJ_RS02170at 412.212 kb on + strand, within MMJJ_RS02170at 412.212 kb on + strand, within MMJJ_RS02170at 412.285 kb on + strand, within MMJJ_RS02170at 412.287 kb on - strand, within MMJJ_RS02170at 412.287 kb on - strand, within MMJJ_RS02170at 412.287 kb on - strand, within MMJJ_RS02170at 412.288 kb on + strand, within MMJJ_RS02170at 412.288 kb on + strand, within MMJJ_RS02170at 412.288 kb on + strand, within MMJJ_RS02170at 412.294 kb on + strand, within MMJJ_RS02170at 412.311 kb on - strand, within MMJJ_RS02170at 412.314 kb on + strand, within MMJJ_RS02170at 412.315 kb on - strand, within MMJJ_RS02170at 412.316 kb on + strand, within MMJJ_RS02170at 412.316 kb on + strand, within MMJJ_RS02170at 412.333 kb on + strand, within MMJJ_RS02170at 412.348 kb on + strand, within MMJJ_RS02170at 412.396 kb on + strand, within MMJJ_RS02170at 412.396 kb on + strand, within MMJJ_RS02170at 412.413 kb on - strand, within MMJJ_RS02170at 412.473 kb on - strand, within MMJJ_RS02170at 412.478 kb on + strand, within MMJJ_RS02170at 412.495 kb on + strand, within MMJJ_RS02170at 412.543 kb on + strandat 412.717 kb on + strandat 413.196 kb on + strandat 413.196 kb on + strandat 413.198 kb on + strandat 413.200 kb on - strandat 413.200 kb on - strandat 413.200 kb on - strandat 413.201 kb on + strandat 413.222 kb on - strandat 413.223 kb on + strandat 413.246 kb on + strandat 413.541 kb on + strand, within MMJJ_RS02180at 413.556 kb on - strand, within MMJJ_RS02180at 413.711 kb on + strand, within MMJJ_RS02180at 413.737 kb on + strand, within MMJJ_RS02180at 413.820 kb on - strand, within MMJJ_RS02180at 413.917 kb on - strand, within MMJJ_RS02180at 413.920 kb on + strand, within MMJJ_RS02180at 413.961 kb on + strand, within MMJJ_RS02180at 414.004 kb on + strand, within MMJJ_RS02180at 414.008 kb on - strand, within MMJJ_RS02180

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM
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411,656 - MMJJ_RS02165 0.73 -1.4
411,657 + MMJJ_RS02165 0.73 -2.1
411,657 + MMJJ_RS02165 0.73 +2.3
411,735 + MMJJ_RS02165 0.83 -0.6
411,854 - -0.2
411,860 + +4.0
411,916 - +1.0
411,991 + -2.2
412,039 - -1.9
412,110 - MMJJ_RS02170 0.13 +1.3
412,149 + MMJJ_RS02170 0.20 +0.4
412,211 - MMJJ_RS02170 0.32 +2.8
412,211 - MMJJ_RS02170 0.32 +0.2
412,212 + MMJJ_RS02170 0.32 -0.9
412,212 + MMJJ_RS02170 0.32 -1.8
412,285 + MMJJ_RS02170 0.45 +1.1
412,287 - MMJJ_RS02170 0.45 +0.1
412,287 - MMJJ_RS02170 0.45 +0.4
412,287 - MMJJ_RS02170 0.45 -3.6
412,288 + MMJJ_RS02170 0.46 +2.4
412,288 + MMJJ_RS02170 0.46 -2.4
412,288 + MMJJ_RS02170 0.46 +1.5
412,294 + MMJJ_RS02170 0.47 -0.8
412,311 - MMJJ_RS02170 0.50 +0.3
412,314 + MMJJ_RS02170 0.50 +0.5
412,315 - MMJJ_RS02170 0.50 +0.2
412,316 + MMJJ_RS02170 0.51 -0.8
412,316 + MMJJ_RS02170 0.51 -2.9
412,333 + MMJJ_RS02170 0.54 +0.5
412,348 + MMJJ_RS02170 0.56 +0.2
412,396 + MMJJ_RS02170 0.65 -2.0
412,396 + MMJJ_RS02170 0.65 +2.3
412,413 - MMJJ_RS02170 0.68 -0.9
412,473 - MMJJ_RS02170 0.79 +1.2
412,478 + MMJJ_RS02170 0.80 -0.3
412,495 + MMJJ_RS02170 0.83 -0.5
412,543 + +0.2
412,717 + -0.8
413,196 + -3.4
413,196 + -2.0
413,198 + -2.2
413,200 - -2.5
413,200 - -0.5
413,200 - +0.4
413,201 + -1.2
413,222 - -0.5
413,223 + -1.8
413,246 + +0.5
413,541 + MMJJ_RS02180 0.23 +1.0
413,556 - MMJJ_RS02180 0.24 +1.9
413,711 + MMJJ_RS02180 0.36 +2.4
413,737 + MMJJ_RS02180 0.38 +0.2
413,820 - MMJJ_RS02180 0.44 +0.8
413,917 - MMJJ_RS02180 0.52 -0.2
413,920 + MMJJ_RS02180 0.52 +0.4
413,961 + MMJJ_RS02180 0.55 +1.4
414,004 + MMJJ_RS02180 0.59 -0.2
414,008 - MMJJ_RS02180 0.59 -1.1

Or see this region's nucleotide sequence