Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01220 and MMJJ_RS01225 are separated by 21 nucleotides MMJJ_RS01225 and MMJJ_RS01230 are separated by 9 nucleotides MMJJ_RS01230 and MMJJ_RS01235 are separated by 126 nucleotides MMJJ_RS01235 and MMJJ_RS01240 are separated by 7 nucleotides
MMJJ_RS01220: MMJJ_RS01220 - exodeoxyribonuclease VII large subunit, at 234,241 to 235,464
_RS01220
MMJJ_RS01225: MMJJ_RS01225 - exodeoxyribonuclease VII small subunit, at 235,486 to 235,689
_RS01225
MMJJ_RS01230: MMJJ_RS01230 - EamA family transporter, at 235,699 to 236,118
_RS01230
MMJJ_RS01235: MMJJ_RS01235 - excinuclease ABC subunit UvrA, at 236,245 to 239,100
_RS01235
MMJJ_RS01240: MMJJ_RS01240 - excinuclease ABC subunit UvrC, at 239,108 to 240,691
_RS01240
Position (kb) 236
237
238
239
240 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 235.310 kb on + strand, within MMJJ_RS01220 at 235.310 kb on + strand, within MMJJ_RS01220 at 235.311 kb on - strand, within MMJJ_RS01220 at 235.332 kb on - strand, within MMJJ_RS01220 at 235.340 kb on + strand, within MMJJ_RS01220 at 235.350 kb on + strand at 235.373 kb on - strand at 235.373 kb on - strand at 235.447 kb on + strand at 235.678 kb on + strand at 235.702 kb on - strand at 235.737 kb on - strand at 235.737 kb on - strand at 235.738 kb on + strand at 235.738 kb on + strand at 235.787 kb on + strand, within MMJJ_RS01230 at 235.803 kb on - strand, within MMJJ_RS01230 at 235.803 kb on - strand, within MMJJ_RS01230 at 235.804 kb on + strand, within MMJJ_RS01230 at 235.842 kb on - strand, within MMJJ_RS01230 at 235.842 kb on - strand, within MMJJ_RS01230 at 235.847 kb on - strand, within MMJJ_RS01230 at 235.871 kb on - strand, within MMJJ_RS01230 at 235.872 kb on + strand, within MMJJ_RS01230 at 235.872 kb on + strand, within MMJJ_RS01230 at 235.903 kb on - strand, within MMJJ_RS01230 at 235.903 kb on - strand, within MMJJ_RS01230 at 235.903 kb on - strand, within MMJJ_RS01230 at 235.903 kb on - strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.947 kb on + strand, within MMJJ_RS01230 at 235.997 kb on + strand, within MMJJ_RS01230 at 236.027 kb on - strand, within MMJJ_RS01230 at 236.113 kb on + strand at 236.143 kb on - strand at 236.144 kb on + strand at 236.144 kb on + strand at 236.145 kb on - strand at 236.145 kb on - strand at 236.145 kb on - strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.147 kb on - strand at 236.188 kb on + strand at 236.199 kb on - strand at 236.199 kb on - strand at 236.199 kb on - strand at 236.199 kb on - strand at 236.202 kb on + strand at 236.240 kb on - strand at 236.241 kb on + strand at 236.245 kb on + strand at 236.253 kb on - strand at 236.254 kb on + strand at 236.261 kb on - strand at 236.280 kb on - strand at 236.281 kb on + strand at 236.286 kb on + strand at 236.325 kb on + strand at 236.330 kb on - strand at 236.364 kb on + strand at 236.373 kb on - strand at 236.374 kb on + strand at 236.379 kb on + strand at 236.380 kb on - strand at 236.381 kb on + strand at 236.401 kb on - strand at 236.402 kb on + strand at 236.402 kb on + strand at 236.423 kb on + strand at 236.452 kb on + strand at 236.466 kb on - strand at 236.470 kb on + strand at 236.617 kb on + strand, within MMJJ_RS01235 at 236.619 kb on - strand, within MMJJ_RS01235 at 236.620 kb on + strand, within MMJJ_RS01235 at 236.648 kb on - strand, within MMJJ_RS01235 at 236.649 kb on + strand, within MMJJ_RS01235 at 236.726 kb on + strand, within MMJJ_RS01235 at 236.933 kb on - strand, within MMJJ_RS01235 at 236.940 kb on + strand, within MMJJ_RS01235 at 236.955 kb on - strand, within MMJJ_RS01235 at 237.002 kb on - strand, within MMJJ_RS01235 at 237.002 kb on - strand, within MMJJ_RS01235 at 237.009 kb on - strand, within MMJJ_RS01235 at 237.010 kb on + strand, within MMJJ_RS01235 at 237.010 kb on + strand, within MMJJ_RS01235 at 237.049 kb on + strand, within MMJJ_RS01235 at 237.073 kb on - strand, within MMJJ_RS01235 at 237.074 kb on + strand, within MMJJ_RS01235 at 237.188 kb on + strand, within MMJJ_RS01235 at 237.248 kb on - strand, within MMJJ_RS01235 at 237.287 kb on + strand, within MMJJ_RS01235 at 237.354 kb on - strand, within MMJJ_RS01235 at 237.516 kb on - strand, within MMJJ_RS01235 at 237.517 kb on + strand, within MMJJ_RS01235 at 237.571 kb on + strand, within MMJJ_RS01235 at 237.675 kb on + strand, within MMJJ_RS01235 at 237.725 kb on - strand, within MMJJ_RS01235 at 237.725 kb on - strand, within MMJJ_RS01235 at 237.768 kb on + strand, within MMJJ_RS01235 at 237.769 kb on - strand, within MMJJ_RS01235 at 237.769 kb on - strand, within MMJJ_RS01235 at 237.773 kb on - strand, within MMJJ_RS01235 at 237.858 kb on - strand, within MMJJ_RS01235 at 237.858 kb on - strand, within MMJJ_RS01235 at 237.858 kb on - strand, within MMJJ_RS01235 at 237.859 kb on + strand, within MMJJ_RS01235 at 237.859 kb on + strand, within MMJJ_RS01235 at 237.907 kb on - strand, within MMJJ_RS01235 at 237.915 kb on + strand, within MMJJ_RS01235 at 238.012 kb on - strand, within MMJJ_RS01235 at 238.181 kb on - strand, within MMJJ_RS01235 at 238.182 kb on + strand, within MMJJ_RS01235 at 238.187 kb on + strand, within MMJJ_RS01235 at 238.268 kb on + strand, within MMJJ_RS01235 at 238.275 kb on - strand, within MMJJ_RS01235 at 238.444 kb on - strand, within MMJJ_RS01235 at 238.445 kb on + strand, within MMJJ_RS01235 at 238.467 kb on - strand, within MMJJ_RS01235 at 238.566 kb on - strand, within MMJJ_RS01235 at 238.566 kb on - strand, within MMJJ_RS01235 at 238.576 kb on + strand, within MMJJ_RS01235 at 238.576 kb on + strand, within MMJJ_RS01235 at 238.634 kb on - strand, within MMJJ_RS01235 at 238.668 kb on - strand, within MMJJ_RS01235 at 238.675 kb on + strand, within MMJJ_RS01235 at 238.693 kb on + strand, within MMJJ_RS01235 at 238.726 kb on - strand, within MMJJ_RS01235 at 238.727 kb on + strand, within MMJJ_RS01235 at 238.731 kb on - strand, within MMJJ_RS01235 at 238.731 kb on - strand, within MMJJ_RS01235 at 238.732 kb on + strand, within MMJJ_RS01235 at 238.737 kb on + strand, within MMJJ_RS01235 at 238.827 kb on + strand at 238.828 kb on - strand at 238.828 kb on - strand at 238.829 kb on + strand at 238.829 kb on + strand at 238.829 kb on + strand at 238.832 kb on - strand at 238.922 kb on - strand at 238.923 kb on + strand at 238.923 kb on + strand at 238.949 kb on - strand at 239.055 kb on + strand at 239.104 kb on - strand at 239.112 kb on - strand at 239.113 kb on + strand at 239.147 kb on - strand at 239.149 kb on - strand at 239.154 kb on + strand at 239.215 kb on - strand at 239.219 kb on + strand at 239.292 kb on - strand, within MMJJ_RS01240 at 239.349 kb on + strand, within MMJJ_RS01240 at 239.360 kb on - strand, within MMJJ_RS01240 at 239.361 kb on + strand, within MMJJ_RS01240 at 239.368 kb on + strand, within MMJJ_RS01240 at 239.374 kb on + strand, within MMJJ_RS01240 at 239.396 kb on - strand, within MMJJ_RS01240 at 239.396 kb on - strand, within MMJJ_RS01240 at 239.396 kb on - strand, within MMJJ_RS01240 at 239.396 kb on - strand, within MMJJ_RS01240 at 239.396 kb on - strand, within MMJJ_RS01240 at 239.397 kb on + strand, within MMJJ_RS01240 at 239.397 kb on + strand, within MMJJ_RS01240 at 239.398 kb on - strand, within MMJJ_RS01240 at 239.403 kb on + strand, within MMJJ_RS01240 at 239.403 kb on + strand, within MMJJ_RS01240 at 239.447 kb on + strand, within MMJJ_RS01240 at 239.479 kb on - strand, within MMJJ_RS01240 at 239.502 kb on + strand, within MMJJ_RS01240 at 239.502 kb on + strand, within MMJJ_RS01240 at 239.581 kb on - strand, within MMJJ_RS01240 at 239.606 kb on + strand, within MMJJ_RS01240 at 239.606 kb on + strand, within MMJJ_RS01240 at 239.815 kb on + strand, within MMJJ_RS01240 at 239.866 kb on - strand, within MMJJ_RS01240 at 239.869 kb on + strand, within MMJJ_RS01240 at 239.964 kb on + strand, within MMJJ_RS01240 at 239.966 kb on - strand, within MMJJ_RS01240 at 239.996 kb on + strand at 239.998 kb on + strand, within MMJJ_RS01240 at 239.998 kb on + strand, within MMJJ_RS01240 at 240.032 kb on + strand, within MMJJ_RS01240 at 240.034 kb on - strand, within MMJJ_RS01240 at 240.034 kb on - strand, within MMJJ_RS01240 at 240.042 kb on + strand, within MMJJ_RS01240 at 240.066 kb on + strand, within MMJJ_RS01240 at 240.066 kb on + strand, within MMJJ_RS01240
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 235,310 + MMJJ_RS01220 0.87 +1.1 235,310 + MMJJ_RS01220 0.87 -0.3 235,311 - MMJJ_RS01220 0.87 +0.6 235,332 - MMJJ_RS01220 0.89 +1.5 235,340 + MMJJ_RS01220 0.90 -2.1 235,350 + +0.6 235,373 - -0.5 235,373 - +0.0 235,447 + -1.2 235,678 + -2.1 235,702 - +0.4 235,737 - +1.8 235,737 - +0.3 235,738 + -0.1 235,738 + +0.4 235,787 + MMJJ_RS01230 0.21 +1.0 235,803 - MMJJ_RS01230 0.25 -1.1 235,803 - MMJJ_RS01230 0.25 -1.7 235,804 + MMJJ_RS01230 0.25 +0.9 235,842 - MMJJ_RS01230 0.34 +0.4 235,842 - MMJJ_RS01230 0.34 -0.7 235,847 - MMJJ_RS01230 0.35 -1.0 235,871 - MMJJ_RS01230 0.41 +1.4 235,872 + MMJJ_RS01230 0.41 -1.8 235,872 + MMJJ_RS01230 0.41 -1.4 235,903 - MMJJ_RS01230 0.49 -1.9 235,903 - MMJJ_RS01230 0.49 -1.8 235,903 - MMJJ_RS01230 0.49 +0.3 235,903 - MMJJ_RS01230 0.49 -0.8 235,904 + MMJJ_RS01230 0.49 -0.4 235,904 + MMJJ_RS01230 0.49 -0.9 235,904 + MMJJ_RS01230 0.49 -1.6 235,904 + MMJJ_RS01230 0.49 -1.1 235,904 + MMJJ_RS01230 0.49 -0.4 235,904 + MMJJ_RS01230 0.49 -0.2 235,947 + MMJJ_RS01230 0.59 +1.1 235,997 + MMJJ_RS01230 0.71 +0.7 236,027 - MMJJ_RS01230 0.78 -2.4 236,113 + +0.2 236,143 - +1.6 236,144 + +1.4 236,144 + +1.4 236,145 - +1.0 236,145 - -3.7 236,145 - +0.8 236,146 + -3.7 236,146 + -0.2 236,146 + -1.3 236,146 + +0.3 236,146 + -2.0 236,146 + -0.6 236,146 + -0.6 236,146 + -1.0 236,146 + +1.1 236,146 + +1.6 236,147 - -0.6 236,188 + -0.4 236,199 - -2.8 236,199 - -1.3 236,199 - +1.0 236,199 - -1.0 236,202 + -1.0 236,240 - -2.4 236,241 + -1.1 236,245 + +2.0 236,253 - +2.1 236,254 + +0.4 236,261 - -0.5 236,280 - +1.4 236,281 + +0.3 236,286 + +0.6 236,325 + -0.4 236,330 - +0.4 236,364 + +0.4 236,373 - -4.0 236,374 + +5.0 236,379 + +0.6 236,380 - -1.6 236,381 + -0.8 236,401 - +1.6 236,402 + +0.8 236,402 + -1.6 236,423 + +3.4 236,452 + +0.5 236,466 - -0.9 236,470 + -1.2 236,617 + MMJJ_RS01235 0.13 -1.1 236,619 - MMJJ_RS01235 0.13 -0.9 236,620 + MMJJ_RS01235 0.13 +1.0 236,648 - MMJJ_RS01235 0.14 -0.0 236,649 + MMJJ_RS01235 0.14 -2.1 236,726 + MMJJ_RS01235 0.17 +0.2 236,933 - MMJJ_RS01235 0.24 -2.2 236,940 + MMJJ_RS01235 0.24 -0.5 236,955 - MMJJ_RS01235 0.25 -1.1 237,002 - MMJJ_RS01235 0.27 +1.2 237,002 - MMJJ_RS01235 0.27 -1.3 237,009 - MMJJ_RS01235 0.27 +0.6 237,010 + MMJJ_RS01235 0.27 +0.5 237,010 + MMJJ_RS01235 0.27 -0.2 237,049 + MMJJ_RS01235 0.28 -0.8 237,073 - MMJJ_RS01235 0.29 +0.0 237,074 + MMJJ_RS01235 0.29 -2.0 237,188 + MMJJ_RS01235 0.33 +0.9 237,248 - MMJJ_RS01235 0.35 +3.1 237,287 + MMJJ_RS01235 0.36 +0.2 237,354 - MMJJ_RS01235 0.39 -1.0 237,516 - MMJJ_RS01235 0.45 +1.2 237,517 + MMJJ_RS01235 0.45 +0.6 237,571 + MMJJ_RS01235 0.46 +0.5 237,675 + MMJJ_RS01235 0.50 -0.9 237,725 - MMJJ_RS01235 0.52 -2.4 237,725 - MMJJ_RS01235 0.52 -2.9 237,768 + MMJJ_RS01235 0.53 -1.0 237,769 - MMJJ_RS01235 0.53 -0.4 237,769 - MMJJ_RS01235 0.53 -0.8 237,773 - MMJJ_RS01235 0.54 -1.0 237,858 - MMJJ_RS01235 0.56 -0.6 237,858 - MMJJ_RS01235 0.56 -0.8 237,858 - MMJJ_RS01235 0.56 -0.1 237,859 + MMJJ_RS01235 0.57 -0.6 237,859 + MMJJ_RS01235 0.57 -2.0 237,907 - MMJJ_RS01235 0.58 +0.8 237,915 + MMJJ_RS01235 0.58 -1.4 238,012 - MMJJ_RS01235 0.62 -0.1 238,181 - MMJJ_RS01235 0.68 +3.5 238,182 + MMJJ_RS01235 0.68 -3.8 238,187 + MMJJ_RS01235 0.68 -0.7 238,268 + MMJJ_RS01235 0.71 -3.1 238,275 - MMJJ_RS01235 0.71 +1.2 238,444 - MMJJ_RS01235 0.77 -0.4 238,445 + MMJJ_RS01235 0.77 -0.3 238,467 - MMJJ_RS01235 0.78 -2.2 238,566 - MMJJ_RS01235 0.81 +0.4 238,566 - MMJJ_RS01235 0.81 -0.2 238,576 + MMJJ_RS01235 0.82 +3.1 238,576 + MMJJ_RS01235 0.82 -1.1 238,634 - MMJJ_RS01235 0.84 -0.6 238,668 - MMJJ_RS01235 0.85 -1.4 238,675 + MMJJ_RS01235 0.85 +0.7 238,693 + MMJJ_RS01235 0.86 +2.4 238,726 - MMJJ_RS01235 0.87 -3.4 238,727 + MMJJ_RS01235 0.87 -1.4 238,731 - MMJJ_RS01235 0.87 +0.7 238,731 - MMJJ_RS01235 0.87 -1.1 238,732 + MMJJ_RS01235 0.87 +0.2 238,737 + MMJJ_RS01235 0.87 +0.5 238,827 + -1.4 238,828 - -2.3 238,828 - +1.1 238,829 + +0.6 238,829 + -0.8 238,829 + +0.7 238,832 - -1.7 238,922 - +1.8 238,923 + +1.1 238,923 + +1.8 238,949 - -1.9 239,055 + +1.0 239,104 - -0.6 239,112 - +1.4 239,113 + -3.0 239,147 - +0.3 239,149 - +0.6 239,154 + -0.8 239,215 - -4.6 239,219 + -0.3 239,292 - MMJJ_RS01240 0.12 -2.4 239,349 + MMJJ_RS01240 0.15 +0.1 239,360 - MMJJ_RS01240 0.16 +0.9 239,361 + MMJJ_RS01240 0.16 -2.0 239,368 + MMJJ_RS01240 0.16 -1.9 239,374 + MMJJ_RS01240 0.17 -4.0 239,396 - MMJJ_RS01240 0.18 -0.6 239,396 - MMJJ_RS01240 0.18 -2.8 239,396 - MMJJ_RS01240 0.18 +1.2 239,396 - MMJJ_RS01240 0.18 +1.2 239,396 - MMJJ_RS01240 0.18 -0.7 239,397 + MMJJ_RS01240 0.18 -2.3 239,397 + MMJJ_RS01240 0.18 +0.2 239,398 - MMJJ_RS01240 0.18 +1.1 239,403 + MMJJ_RS01240 0.19 -2.5 239,403 + MMJJ_RS01240 0.19 +0.6 239,447 + MMJJ_RS01240 0.21 -3.2 239,479 - MMJJ_RS01240 0.23 -0.4 239,502 + MMJJ_RS01240 0.25 -3.5 239,502 + MMJJ_RS01240 0.25 -1.2 239,581 - MMJJ_RS01240 0.30 +0.7 239,606 + MMJJ_RS01240 0.31 +0.2 239,606 + MMJJ_RS01240 0.31 +1.9 239,815 + MMJJ_RS01240 0.45 -1.4 239,866 - MMJJ_RS01240 0.48 -0.5 239,869 + MMJJ_RS01240 0.48 +0.1 239,964 + MMJJ_RS01240 0.54 +0.6 239,966 - MMJJ_RS01240 0.54 -2.4 239,996 + +0.6 239,998 + MMJJ_RS01240 0.56 -0.7 239,998 + MMJJ_RS01240 0.56 -0.9 240,032 + MMJJ_RS01240 0.58 +2.3 240,034 - MMJJ_RS01240 0.58 +0.6 240,034 - MMJJ_RS01240 0.58 +2.6 240,042 + MMJJ_RS01240 0.59 +0.3 240,066 + MMJJ_RS01240 0.60 +0.1 240,066 + MMJJ_RS01240 0.60 +2.8
Or see this region's nucleotide sequence