Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00405 and MMJJ_RS00410 are separated by 43 nucleotides MMJJ_RS00410 and MMJJ_RS00415 are separated by 43 nucleotides MMJJ_RS00415 and MMJJ_RS00420 are separated by 14 nucleotides MMJJ_RS00420 and MMJJ_RS00425 are separated by 185 nucleotides
MMJJ_RS00405: MMJJ_RS00405 - flavodoxin family protein, at 75,364 to 75,945
_RS00405
MMJJ_RS00410: MMJJ_RS00410 - hypothetical protein, at 75,989 to 76,417
_RS00410
MMJJ_RS00415: MMJJ_RS00415 - DedA family protein, at 76,461 to 76,943
_RS00415
MMJJ_RS00420: MMJJ_RS00420 - CBS domain-containing protein, at 76,958 to 77,338
_RS00420
MMJJ_RS00425: MMJJ_RS00425 - hypothetical protein, at 77,524 to 77,916
_RS00425
Position (kb) 76
77 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 75.472 kb on + strand, within MMJJ_RS00405 at 75.494 kb on - strand, within MMJJ_RS00405 at 75.540 kb on - strand, within MMJJ_RS00405 at 75.540 kb on - strand, within MMJJ_RS00405 at 75.541 kb on + strand, within MMJJ_RS00405 at 75.541 kb on + strand, within MMJJ_RS00405 at 75.541 kb on + strand, within MMJJ_RS00405 at 75.548 kb on - strand, within MMJJ_RS00405 at 75.596 kb on - strand, within MMJJ_RS00405 at 75.596 kb on - strand, within MMJJ_RS00405 at 75.616 kb on - strand, within MMJJ_RS00405 at 75.616 kb on - strand, within MMJJ_RS00405 at 75.685 kb on + strand, within MMJJ_RS00405 at 75.754 kb on + strand, within MMJJ_RS00405 at 75.781 kb on + strand, within MMJJ_RS00405 at 75.827 kb on + strand, within MMJJ_RS00405 at 75.941 kb on - strand at 75.942 kb on + strand at 75.943 kb on - strand at 75.946 kb on - strand at 75.947 kb on + strand at 75.966 kb on - strand at 75.976 kb on - strand at 76.026 kb on - strand at 76.030 kb on + strand at 76.077 kb on - strand, within MMJJ_RS00410 at 76.082 kb on + strand, within MMJJ_RS00410 at 76.085 kb on + strand, within MMJJ_RS00410 at 76.086 kb on - strand, within MMJJ_RS00410 at 76.086 kb on - strand, within MMJJ_RS00410 at 76.086 kb on - strand, within MMJJ_RS00410 at 76.086 kb on - strand, within MMJJ_RS00410 at 76.102 kb on - strand, within MMJJ_RS00410 at 76.152 kb on - strand, within MMJJ_RS00410 at 76.198 kb on - strand, within MMJJ_RS00410 at 76.198 kb on - strand, within MMJJ_RS00410 at 76.251 kb on - strand, within MMJJ_RS00410 at 76.252 kb on + strand, within MMJJ_RS00410 at 76.268 kb on - strand, within MMJJ_RS00410 at 76.268 kb on - strand, within MMJJ_RS00410 at 76.269 kb on + strand, within MMJJ_RS00410 at 76.269 kb on + strand, within MMJJ_RS00410 at 76.269 kb on + strand, within MMJJ_RS00410 at 76.269 kb on + strand, within MMJJ_RS00410 at 76.292 kb on + strand, within MMJJ_RS00410 at 76.292 kb on + strand, within MMJJ_RS00410 at 76.292 kb on + strand, within MMJJ_RS00410 at 76.299 kb on + strand, within MMJJ_RS00410 at 76.299 kb on + strand, within MMJJ_RS00410 at 76.416 kb on + strand at 76.416 kb on + strand at 76.416 kb on + strand at 76.424 kb on + strand at 76.458 kb on + strand at 76.458 kb on + strand at 76.463 kb on + strand at 76.463 kb on + strand at 76.482 kb on - strand at 76.483 kb on + strand at 76.513 kb on + strand, within MMJJ_RS00415 at 76.519 kb on + strand, within MMJJ_RS00415 at 76.582 kb on + strand, within MMJJ_RS00415 at 76.647 kb on - strand, within MMJJ_RS00415 at 76.647 kb on - strand, within MMJJ_RS00415 at 76.680 kb on + strand, within MMJJ_RS00415 at 76.751 kb on - strand, within MMJJ_RS00415 at 76.751 kb on - strand, within MMJJ_RS00415 at 76.763 kb on - strand, within MMJJ_RS00415 at 76.783 kb on + strand, within MMJJ_RS00415 at 76.833 kb on + strand, within MMJJ_RS00415 at 76.833 kb on + strand, within MMJJ_RS00415 at 76.836 kb on - strand, within MMJJ_RS00415 at 76.837 kb on + strand, within MMJJ_RS00415 at 76.837 kb on + strand, within MMJJ_RS00415 at 76.838 kb on - strand, within MMJJ_RS00415 at 76.839 kb on + strand, within MMJJ_RS00415 at 76.839 kb on + strand, within MMJJ_RS00415 at 76.839 kb on + strand, within MMJJ_RS00415 at 76.845 kb on - strand, within MMJJ_RS00415 at 76.903 kb on - strand at 76.908 kb on + strand at 76.908 kb on + strand at 76.911 kb on - strand at 76.982 kb on - strand at 76.998 kb on + strand, within MMJJ_RS00420 at 76.999 kb on - strand, within MMJJ_RS00420 at 77.030 kb on - strand, within MMJJ_RS00420 at 77.045 kb on - strand, within MMJJ_RS00420 at 77.108 kb on - strand, within MMJJ_RS00420 at 77.138 kb on - strand, within MMJJ_RS00420 at 77.138 kb on - strand, within MMJJ_RS00420 at 77.138 kb on - strand, within MMJJ_RS00420 at 77.139 kb on + strand, within MMJJ_RS00420 at 77.139 kb on + strand, within MMJJ_RS00420 at 77.199 kb on + strand, within MMJJ_RS00420 at 77.199 kb on + strand, within MMJJ_RS00420 at 77.199 kb on + strand, within MMJJ_RS00420 at 77.200 kb on - strand, within MMJJ_RS00420 at 77.222 kb on - strand, within MMJJ_RS00420 at 77.222 kb on - strand, within MMJJ_RS00420 at 77.223 kb on + strand, within MMJJ_RS00420 at 77.228 kb on - strand, within MMJJ_RS00420 at 77.229 kb on + strand, within MMJJ_RS00420 at 77.252 kb on + strand, within MMJJ_RS00420 at 77.262 kb on + strand, within MMJJ_RS00420 at 77.266 kb on - strand, within MMJJ_RS00420 at 77.367 kb on - strand at 77.370 kb on - strand at 77.370 kb on - strand at 77.371 kb on + strand at 77.372 kb on - strand at 77.372 kb on - strand at 77.373 kb on + strand at 77.373 kb on + strand at 77.374 kb on - strand at 77.374 kb on - strand at 77.375 kb on + strand at 77.379 kb on + strand at 77.427 kb on + strand at 77.441 kb on - strand at 77.441 kb on - strand at 77.445 kb on - strand at 77.446 kb on + strand at 77.446 kb on + strand at 77.446 kb on + strand at 77.446 kb on + strand at 77.446 kb on + strand at 77.447 kb on - strand at 77.451 kb on + strand at 77.456 kb on + strand at 77.465 kb on - strand at 77.469 kb on + strand at 77.469 kb on + strand at 77.484 kb on - strand at 77.484 kb on - strand at 77.485 kb on + strand at 77.485 kb on + strand at 77.485 kb on + strand at 77.486 kb on - strand at 77.486 kb on - strand at 77.486 kb on - strand at 77.486 kb on - strand at 77.486 kb on - strand at 77.486 kb on - strand at 77.486 kb on - strand at 77.487 kb on + strand at 77.487 kb on + strand at 77.487 kb on + strand at 77.487 kb on + strand at 77.487 kb on + strand at 77.488 kb on - strand at 77.488 kb on - strand at 77.489 kb on + strand at 77.489 kb on + strand at 77.489 kb on + strand at 77.489 kb on + strand at 77.500 kb on - strand at 77.500 kb on - strand at 77.506 kb on + strand at 77.541 kb on - strand at 77.541 kb on - strand at 77.541 kb on - strand at 77.541 kb on - strand at 77.542 kb on + strand at 77.542 kb on + strand at 77.542 kb on + strand at 77.544 kb on - strand at 77.562 kb on - strand at 77.563 kb on + strand at 77.563 kb on + strand at 77.577 kb on - strand, within MMJJ_RS00425 at 77.620 kb on - strand, within MMJJ_RS00425 at 77.621 kb on + strand, within MMJJ_RS00425 at 77.621 kb on + strand, within MMJJ_RS00425 at 77.675 kb on - strand, within MMJJ_RS00425 at 77.874 kb on + strand, within MMJJ_RS00425 at 77.927 kb on - strand at 77.932 kb on - strand at 77.933 kb on + strand at 77.933 kb on + strand at 77.933 kb on + strand at 77.934 kb on - strand at 77.935 kb on + strand at 77.935 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 75,472 + MMJJ_RS00405 0.19 +0.8 75,494 - MMJJ_RS00405 0.22 -3.3 75,540 - MMJJ_RS00405 0.30 -0.4 75,540 - MMJJ_RS00405 0.30 +2.7 75,541 + MMJJ_RS00405 0.30 +2.1 75,541 + MMJJ_RS00405 0.30 -1.4 75,541 + MMJJ_RS00405 0.30 +1.6 75,548 - MMJJ_RS00405 0.32 +2.9 75,596 - MMJJ_RS00405 0.40 -2.6 75,596 - MMJJ_RS00405 0.40 +2.8 75,616 - MMJJ_RS00405 0.43 +0.0 75,616 - MMJJ_RS00405 0.43 +1.0 75,685 + MMJJ_RS00405 0.55 +0.1 75,754 + MMJJ_RS00405 0.67 -1.3 75,781 + MMJJ_RS00405 0.72 +1.8 75,827 + MMJJ_RS00405 0.80 -0.7 75,941 - +0.1 75,942 + +1.3 75,943 - -2.2 75,946 - -1.3 75,947 + -0.4 75,966 - -0.4 75,976 - +1.4 76,026 - +0.5 76,030 + -3.2 76,077 - MMJJ_RS00410 0.21 -0.2 76,082 + MMJJ_RS00410 0.22 +1.4 76,085 + MMJJ_RS00410 0.22 -4.1 76,086 - MMJJ_RS00410 0.23 -3.1 76,086 - MMJJ_RS00410 0.23 +1.5 76,086 - MMJJ_RS00410 0.23 -3.1 76,086 - MMJJ_RS00410 0.23 -0.5 76,102 - MMJJ_RS00410 0.26 -0.0 76,152 - MMJJ_RS00410 0.38 +0.8 76,198 - MMJJ_RS00410 0.49 -0.8 76,198 - MMJJ_RS00410 0.49 +1.1 76,251 - MMJJ_RS00410 0.61 -0.1 76,252 + MMJJ_RS00410 0.61 -1.1 76,268 - MMJJ_RS00410 0.65 -0.0 76,268 - MMJJ_RS00410 0.65 +1.4 76,269 + MMJJ_RS00410 0.65 -2.4 76,269 + MMJJ_RS00410 0.65 -0.5 76,269 + MMJJ_RS00410 0.65 -1.2 76,269 + MMJJ_RS00410 0.65 -3.7 76,292 + MMJJ_RS00410 0.71 +2.1 76,292 + MMJJ_RS00410 0.71 +0.7 76,292 + MMJJ_RS00410 0.71 +0.8 76,299 + MMJJ_RS00410 0.72 -1.6 76,299 + MMJJ_RS00410 0.72 +3.0 76,416 + -0.7 76,416 + -0.1 76,416 + +1.1 76,424 + -0.4 76,458 + +1.6 76,458 + -4.0 76,463 + -1.8 76,463 + -1.1 76,482 - +1.5 76,483 + -2.2 76,513 + MMJJ_RS00415 0.11 -2.2 76,519 + MMJJ_RS00415 0.12 -1.0 76,582 + MMJJ_RS00415 0.25 -2.6 76,647 - MMJJ_RS00415 0.39 -1.7 76,647 - MMJJ_RS00415 0.39 -1.6 76,680 + MMJJ_RS00415 0.45 -0.9 76,751 - MMJJ_RS00415 0.60 -0.4 76,751 - MMJJ_RS00415 0.60 +1.0 76,763 - MMJJ_RS00415 0.63 -1.7 76,783 + MMJJ_RS00415 0.67 -2.7 76,833 + MMJJ_RS00415 0.77 -2.4 76,833 + MMJJ_RS00415 0.77 -2.7 76,836 - MMJJ_RS00415 0.78 -0.8 76,837 + MMJJ_RS00415 0.78 -2.7 76,837 + MMJJ_RS00415 0.78 -0.4 76,838 - MMJJ_RS00415 0.78 -2.7 76,839 + MMJJ_RS00415 0.78 +0.7 76,839 + MMJJ_RS00415 0.78 -3.8 76,839 + MMJJ_RS00415 0.78 -2.4 76,845 - MMJJ_RS00415 0.80 -4.3 76,903 - +0.4 76,908 + -2.0 76,908 + -0.4 76,911 - +0.2 76,982 - -1.3 76,998 + MMJJ_RS00420 0.10 -3.8 76,999 - MMJJ_RS00420 0.11 +0.8 77,030 - MMJJ_RS00420 0.19 -2.0 77,045 - MMJJ_RS00420 0.23 +1.8 77,108 - MMJJ_RS00420 0.39 -1.0 77,138 - MMJJ_RS00420 0.47 -3.7 77,138 - MMJJ_RS00420 0.47 -2.7 77,138 - MMJJ_RS00420 0.47 -3.9 77,139 + MMJJ_RS00420 0.48 +3.4 77,139 + MMJJ_RS00420 0.48 +0.6 77,199 + MMJJ_RS00420 0.63 -0.7 77,199 + MMJJ_RS00420 0.63 -2.0 77,199 + MMJJ_RS00420 0.63 -0.8 77,200 - MMJJ_RS00420 0.64 -4.1 77,222 - MMJJ_RS00420 0.69 -0.4 77,222 - MMJJ_RS00420 0.69 -2.6 77,223 + MMJJ_RS00420 0.70 +0.5 77,228 - MMJJ_RS00420 0.71 -1.2 77,229 + MMJJ_RS00420 0.71 -2.6 77,252 + MMJJ_RS00420 0.77 -2.2 77,262 + MMJJ_RS00420 0.80 +4.1 77,266 - MMJJ_RS00420 0.81 -0.4 77,367 - +1.1 77,370 - -1.0 77,370 - +2.1 77,371 + +0.6 77,372 - -0.1 77,372 - +0.9 77,373 + -0.9 77,373 + +1.2 77,374 - -1.3 77,374 - +1.0 77,375 + -3.1 77,379 + +2.3 77,427 + -1.0 77,441 - -3.7 77,441 - -4.4 77,445 - -2.2 77,446 + -1.9 77,446 + -0.7 77,446 + -0.3 77,446 + -1.0 77,446 + -3.2 77,447 - -2.2 77,451 + +0.8 77,456 + -3.1 77,465 - -1.2 77,469 + +4.1 77,469 + -1.8 77,484 - -2.2 77,484 - +1.4 77,485 + -2.7 77,485 + -0.1 77,485 + +0.1 77,486 - +0.2 77,486 - -2.2 77,486 - -3.7 77,486 - -3.6 77,486 - -0.7 77,486 - +0.6 77,486 - -2.7 77,487 + -1.0 77,487 + -0.0 77,487 + -1.4 77,487 + -0.3 77,487 + -0.7 77,488 - -1.7 77,488 - +0.6 77,489 + -0.7 77,489 + -1.4 77,489 + -3.6 77,489 + -0.7 77,500 - -0.3 77,500 - -1.0 77,506 + -0.2 77,541 - -1.4 77,541 - -0.7 77,541 - -0.8 77,541 - -3.3 77,542 + -3.0 77,542 + -0.2 77,542 + -2.1 77,544 - -1.0 77,562 - -3.4 77,563 + -2.9 77,563 + -0.1 77,577 - MMJJ_RS00425 0.13 -0.0 77,620 - MMJJ_RS00425 0.24 -4.1 77,621 + MMJJ_RS00425 0.25 -1.6 77,621 + MMJJ_RS00425 0.25 -0.4 77,675 - MMJJ_RS00425 0.38 -1.6 77,874 + MMJJ_RS00425 0.89 -2.1 77,927 - -0.4 77,932 - -0.0 77,933 + -4.7 77,933 + +0.2 77,933 + -0.7 77,934 - -1.0 77,935 + -2.2 77,935 + -1.7
Or see this region's nucleotide sequence